National Institutes of Health/Office of the Director
DP2 GM123494
米国
引用
ジャーナル: Proc Natl Acad Sci U S A / 年: 2021 タイトル: Entropy-regularized deconvolution of cellular cryotransmission electron tomograms. 著者: Matthew Croxford / Michael Elbaum / Muthuvel Arigovindan / Zvi Kam / David Agard / Elizabeth Villa / John Sedat / 要旨: Cryo-electron tomography (cryo-ET) allows for the high-resolution visualization of biological macromolecules. However, the technique is limited by a low signal-to-noise ratio (SNR) and variance in ...Cryo-electron tomography (cryo-ET) allows for the high-resolution visualization of biological macromolecules. However, the technique is limited by a low signal-to-noise ratio (SNR) and variance in contrast at different frequencies, as well as reduced Z resolution. Here, we applied entropy-regularized deconvolution (ER-DC) to cryo-ET data generated from transmission electron microscopy (TEM) and reconstructed using weighted back projection (WBP). We applied deconvolution to several in situ cryo-ET datasets and assessed the results by Fourier analysis and subtomogram analysis (STA).
EMPIAR-10761 (タイトル: Entropy Regularized Deconvolution of Cellular Cryo-Transmission Electron Tomograms Data size: 6.4 Data #1: Aligned tilt series, back projection, and corresponding deconvolution of a HEK293 cell [tilt series])
集束イオンビーム - 装置: OTHER / 集束イオンビーム - イオン: OTHER / 集束イオンビーム - 電圧: 30 kV / 集束イオンビーム - 電流: 0.03 nA / 集束イオンビーム - 時間: 1000 sec. / 集束イオンビーム - 温度: 83 K / 集束イオンビーム - Initial thickness: 5000 nm / 集束イオンビーム - 最終 厚さ: 200 nm 集束イオンビーム - 詳細: The value given for _emd_sectioning_focused_ion_beam.instrument is FEI Scios FIB. This is not in a list of allowed values {'DB235', 'OTHER'} so OTHER is written into the XML file.
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電子顕微鏡法
顕微鏡
FEI POLARA 300
撮影
フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 100.0 e/Å2