National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
5R01GM127571-04
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
5R35GM118099-05
United States
Spanish National Research Council
SAF2017-84565-R
Spain
Citation
Journal: Life Sci Alliance / Year: 2022 Title: Electron cryo-tomography structure of axonemal doublet microtubule from . Authors: Sam Li / Jose-Jesus Fernandez / Amy S Fabritius / David A Agard / Mark Winey / Abstract: Doublet microtubules (DMTs) provide a scaffold for axoneme assembly in motile cilia. Aside from α/β tubulins, the DMT comprises a large number of non-tubulin proteins in the luminal wall of DMTs, ...Doublet microtubules (DMTs) provide a scaffold for axoneme assembly in motile cilia. Aside from α/β tubulins, the DMT comprises a large number of non-tubulin proteins in the luminal wall of DMTs, collectively named the microtubule inner proteins (MIPs). We used cryoET to study axoneme DMT isolated from We present the structures of DMT at nanometer and sub-nanometer resolution. The structures confirm that MIP RIB72A/B binds to the luminal wall of DMT by multiple DM10 domains. We found FAP115, an MIP-containing multiple EF-hand domains, located at the interface of four-tubulin dimers in the lumen of A-tubule. It contacts both lateral and longitudinal tubulin interfaces and playing a critical role in DMT stability. We observed substantial structure heterogeneity in DMT in an knockout strain, showing extensive structural defects beyond the FAP115-binding site. The defects propagate along the axoneme. Finally, by comparing DMT structures from and , we have identified a number of conserved MIPs as well as MIPs that are unique to each organism. This conservation and diversity of the DMT structures might be linked to their specific functions. Our work provides structural insights essential for understanding the roles of MIPs during motile cilium assembly and function, as well as their relationships to human ciliopathies.
History
Deposition
Jul 2, 2021
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Header (metadata) release
Dec 29, 2021
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Map release
Dec 29, 2021
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Update
Jan 19, 2022
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Current status
Jan 19, 2022
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
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