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Yorodumi- EMDB-23488: Hendra virus receptor binding protein in complex with HENV-103 an... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-23488 | |||||||||
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Title | Hendra virus receptor binding protein in complex with HENV-103 and HENV-117 Fabs | |||||||||
Map data | ||||||||||
Sample |
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Biological species | Hendra henipavirus | |||||||||
Method | single particle reconstruction / negative staining / Resolution: 15.0 Å | |||||||||
Authors | Binshtein E / Crowe JE | |||||||||
Funding support | United States, 1 items
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Citation | Journal: Cell Rep / Year: 2021 Title: Cooperativity mediated by rationally selected combinations of human monoclonal antibodies targeting the henipavirus receptor binding protein. Authors: Michael P Doyle / Nurgun Kose / Viktoriya Borisevich / Elad Binshtein / Moushimi Amaya / Marcus Nagel / Edward J Annand / Erica Armstrong / Robin Bombardi / Jinhui Dong / Kevin L Schey / ...Authors: Michael P Doyle / Nurgun Kose / Viktoriya Borisevich / Elad Binshtein / Moushimi Amaya / Marcus Nagel / Edward J Annand / Erica Armstrong / Robin Bombardi / Jinhui Dong / Kevin L Schey / Christopher C Broder / Larry Zeitlin / Erin A Kuang / Zachary A Bornholdt / Brandyn R West / Thomas W Geisbert / Robert W Cross / James E Crowe / Abstract: Hendra virus and Nipah virus (NiV), members of the Henipavirus (HNV) genus, are zoonotic paramyxoviruses known to cause severe disease across six mammalian orders, including humans. We isolated a ...Hendra virus and Nipah virus (NiV), members of the Henipavirus (HNV) genus, are zoonotic paramyxoviruses known to cause severe disease across six mammalian orders, including humans. We isolated a panel of human monoclonal antibodies (mAbs) from the B cells of an individual with prior exposure to equine Hendra virus (HeV) vaccine, targeting distinct antigenic sites. The most potent class of cross-reactive antibodies achieves neutralization by blocking viral attachment to the host cell receptors ephrin-B2 and ephrin-B3, with a second class being enhanced by receptor binding. mAbs from both classes display synergistic activity in vitro. In a stringent hamster model of NiV Bangladesh (NiV) infection, antibodies from both classes reduce morbidity and mortality and achieve synergistic protection in combination. These candidate mAbs might be suitable for use in a cocktail therapeutic approach to achieve synergistic potency and reduce the risk of virus escape. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_23488.map.gz | 7.6 MB | EMDB map data format | |
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Header (meta data) | emd-23488-v30.xml emd-23488.xml | 9.7 KB 9.7 KB | Display Display | EMDB header |
Images | emd_23488.png | 19 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-23488 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-23488 | HTTPS FTP |
-Validation report
Summary document | emd_23488_validation.pdf.gz | 294 KB | Display | EMDB validaton report |
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Full document | emd_23488_full_validation.pdf.gz | 293.5 KB | Display | |
Data in XML | emd_23488_validation.xml.gz | 5.6 KB | Display | |
Data in CIF | emd_23488_validation.cif.gz | 6.4 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-23488 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-23488 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_23488.map.gz / Format: CCP4 / Size: 8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 3.514 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : HeV RBP with 2 Fabs
Entire | Name: HeV RBP with 2 Fabs |
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Components |
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-Supramolecule #1: HeV RBP with 2 Fabs
Supramolecule | Name: HeV RBP with 2 Fabs / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Hendra henipavirus |
Recombinant expression | Organism: Homo sapiens (human) |
-Experimental details
-Structure determination
Method | negative staining |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Staining | Type: NEGATIVE / Material: Uranyl Formate |
Grid | Model: Homemade / Support film - Material: CARBON / Pretreatment - Type: GLOW DISCHARGE |
-Electron microscopy
Microscope | FEI TECNAI F20 |
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Image recording | Film or detector model: GATAN ULTRASCAN 4000 (4k x 4k) / Number grids imaged: 1 / Number real images: 377 / Average exposure time: 1.0 sec. / Average electron dose: 35.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | C2 aperture diameter: 100.0 µm / Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Cs: 2.2 mm / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 62000 |
Experimental equipment | Model: Tecnai F20 / Image courtesy: FEI Company |