ジャーナル: Curr Biol / 年: 2013 タイトル: Native architecture of the centriole proximal region reveals features underlying its 9-fold radial symmetry. 著者: Paul Guichard / Virginie Hachet / Norbert Majubu / Aitana Neves / Davide Demurtas / Natacha Olieric / Isabelle Fluckiger / Akinori Yamada / Kumiko Kihara / Yuichiro Nishida / Shigeharu Moriya ...著者: Paul Guichard / Virginie Hachet / Norbert Majubu / Aitana Neves / Davide Demurtas / Natacha Olieric / Isabelle Fluckiger / Akinori Yamada / Kumiko Kihara / Yuichiro Nishida / Shigeharu Moriya / Michel O Steinmetz / Yuichi Hongoh / Pierre Gönczy / 要旨: BACKGROUND: Centrioles are cylindrical microtubule-based structures whose assembly is critical for the formation of cilia, flagella, and centrosomes. The centriole proximal region harbors a cartwheel ...BACKGROUND: Centrioles are cylindrical microtubule-based structures whose assembly is critical for the formation of cilia, flagella, and centrosomes. The centriole proximal region harbors a cartwheel that dictates the 9-fold symmetry of centrioles. Although the cartwheel architecture has been recently analyzed, how it connects to the peripheral microtubules is not understood. More generally, a high-resolution view of the proximal region of the centriole is lacking, thus limiting understanding of the underlying assembly mechanisms. RESULTS: We report the complete architecture of the Trichonympha centriole proximal region using cryotomography. The resulting 3D map reveals several features, including additional densities in the ...RESULTS: We report the complete architecture of the Trichonympha centriole proximal region using cryotomography. The resulting 3D map reveals several features, including additional densities in the cartwheel that exhibit a 9-fold symmetrical arrangement, as well as the structure of the Pinhead and the A-C linker that connect to microtubules. Moreover, we uncover striking chiral features that might impart directionality to the entire centriole. Furthermore, we identify Trichonympha SAS-6 and demonstrate that it localizes to the cartwheel in vivo. CONCLUSIONS: Our work provides unprecedented insight into the architecture of the centriole proximal region, which is key for a thorough understanding of the mechanisms governing centriole assembly.
ダウンロード / ファイル: emd_2330.map.gz / 形式: CCP4 / 大きさ: 3.4 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
electron cryo-tomography reconstruction and subvolume averaging of the proximal basal body triplet from Trichonympha.
ボクセルのサイズ
X=Y=Z: 7.5 Å
密度
表面レベル
By EMDB: 0.0 / ムービー #1: 0.4
最小 - 最大
-3.1838901 - 2.45359182
平均 (標準偏差)
-0.90669858 (±0.93229675)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
124
111
66
Spacing
124
111
66
セル
A: 832.5 Å / B: 930.0 Å / C: 495.0 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
7.5
7.5
7.5
M x/y/z
111
124
66
origin x/y/z
0.000
0.000
0.000
length x/y/z
832.500
930.000
495.000
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-27
-15
-36
NX/NY/NZ
55
31
73
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
111
124
66
D min/max/mean
-3.184
2.454
-0.907
-
添付データ
-
試料の構成要素
-
全体 : electron cryo-tomography reconstruction and subvolume averaging o...
全体
名称: electron cryo-tomography reconstruction and subvolume averaging of the proximal basal body triplet from trichonympha
要素
試料: electron cryo-tomography reconstruction and subvolume averaging of the proximal basal body triplet from trichonympha
細胞器官・細胞要素: Basal body
-
超分子 #1000: electron cryo-tomography reconstruction and subvolume averaging o...
超分子
名称: electron cryo-tomography reconstruction and subvolume averaging of the proximal basal body triplet from trichonympha タイプ: sample / ID: 1000 / Number unique components: 1
-
超分子 #1: Basal body
超分子
名称: Basal body / タイプ: organelle_or_cellular_component / ID: 1 / Name.synonym: centriole / 組換発現: No