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- EMDB-22976: Subtomogram average of viral spikes of intracellular viruses from... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-22976 | |||||||||
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Title | Subtomogram average of viral spikes of intracellular viruses from cell lamellae | |||||||||
![]() | Subtomogram averaging of in cell SARS-Cov-2 spikes | |||||||||
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Biological species | ![]() ![]() | |||||||||
Method | subtomogram averaging / cryo EM / Resolution: 11.0 Å | |||||||||
![]() | Sun DP / Zhang PJ | |||||||||
![]() | ![]() Title: SARS-CoV-2 Assembly and Egress Pathway Revealed by Correlative Multi-modal 1 Multi-scale Cryo-imaging Authors: Zhang PJ / Sun DP | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 500.3 KB | ![]() | |
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Header (meta data) | ![]() ![]() | 6.6 KB 6.6 KB | Display Display | ![]() |
Images | ![]() | 48.1 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 286.2 KB | Display | ![]() |
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Full document | ![]() | 285.8 KB | Display | |
Data in XML | ![]() | 5.3 KB | Display | |
Data in CIF | ![]() | 5.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Subtomogram averaging of in cell SARS-Cov-2 spikes | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 4.26 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Cov-2 viral spikes
Entire | Name: Cov-2 viral spikes |
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Components |
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-Supramolecule #1: Cov-2 viral spikes
Supramolecule | Name: Cov-2 viral spikes / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | subtomogram averaging |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 3.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Applied symmetry - Point group: C3 (3 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 11.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number subtomograms used: 450 |
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Extraction | Number tomograms: 2 / Number images used: 450 |
Final angle assignment | Type: PROJECTION MATCHING |