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- EMDB-22398: Free M complex -

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Open data


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Basic information

Entry
Database: EMDB / ID: EMD-22398
TitleFree M complex
Map dataFree M complex
Sample
  • Complex: Free M complex
Biological speciesChaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 6.6 Å
AuthorsZhang HQ / Chen DC
CitationJournal: To Be Published
Title: Free M complex
Authors: Zhang HQ / Chen DC
History
DepositionAug 2, 2020-
Header (metadata) releaseFeb 17, 2021-
Map releaseFeb 17, 2021-
UpdateFeb 17, 2021-
Current statusFeb 17, 2021Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.188
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by radius
  • Surface level: 0.188
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_22398.png

Downloads & links

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Map

FileDownload / File: emd_22398.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationFree M complex
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.1 Å/pix.
x 480 pix.
= 526.08 Å		1.1 Å/pix.
x 480 pix.
= 526.08 Å		1.1 Å/pix.
x 480 pix.
= 526.08 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.096 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.188 / Movie #1: 0.188
Minimum - Maximum-0.52953583 - 1.4389797
Average (Standard dev.)0.006134475 (±0.045703247)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 526.07996 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0961.0961.096
M x/y/z480480480
origin x/y/z0.0000.0000.000
length x/y/z526.080526.080526.080
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS480480480
D min/max/mean-0.5301.4390.006

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Supplemental data

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Sample components

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Entire : Free M complex

EntireName: Free M complex
Components
  • Complex: Free M complex

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Supramolecule #1: Free M complex

SupramoleculeName: Free M complex / type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: OTHER
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionResolution.type: BY AUTHOR / Resolution: 6.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 69332
Initial angle assignmentType: OTHER
Final angle assignmentType: OTHER
FSC plot (resolution estimation)

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