National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
F32GM126760
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01AI147625
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01GM111795
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
U24GM116792
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
S10OD018111
United States
National Science Foundation (NSF, United States)
DMR-1548924
United States
National Science Foundation (NSF, United States)
DBI-1338135
United States
Citation
Journal: Elife / Year: 2020 Title: Structural basis for capsid recruitment and coat formation during HSV-1 nuclear egress. Authors: Elizabeth B Draganova / Jiayan Zhang / Z Hong Zhou / Ekaterina E Heldwein / Abstract: During herpesvirus infection, egress of nascent viral capsids from the nucleus is mediated by the viral nuclear egress complex (NEC). NEC deforms the inner nuclear membrane (INM) around the capsid by ...During herpesvirus infection, egress of nascent viral capsids from the nucleus is mediated by the viral nuclear egress complex (NEC). NEC deforms the inner nuclear membrane (INM) around the capsid by forming a hexagonal array. However, how the NEC coat interacts with the capsid and how curved coats are generated to enable budding is yet unclear. Here, by structure-guided truncations, confocal microscopy, and cryoelectron tomography, we show that binding of the capsid protein UL25 promotes the formation of NEC pentagons rather than hexagons. We hypothesize that during nuclear budding, binding of UL25 situated at the pentagonal capsid vertices to the NEC at the INM promotes formation of NEC pentagons that would anchor the NEC coat to the capsid. Incorporation of NEC pentagons at the points of contact with the vertices would also promote assembly of the curved hexagonal NEC coat around the capsid, leading to productive egress of UL25-decorated capsids.
History
Deposition
Jun 23, 2020
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Header (metadata) release
Jul 8, 2020
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Map release
Jul 8, 2020
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Update
Jul 8, 2020
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Current status
Jul 8, 2020
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
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