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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-21146 | |||||||||
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Title | Cryo-EM structure of Cascade-TniQ-dsDNA ternary complex | |||||||||
![]() | Cascade-TniQ-dsDNA ternary complex | |||||||||
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![]() | CRISPR-Cas system / Cascade- TniQ / IMMUNE SYSTEM | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.2 Å | |||||||||
![]() | Jia N / Patel DJ | |||||||||
![]() | ![]() Title: Structure-function insights into the initial step of DNA integration by a CRISPR-Cas-Transposon complex. Authors: Ning Jia / Wei Xie / M Jason de la Cruz / Edward T Eng / Dinshaw J Patel / ![]() | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 7.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 17.8 KB 17.8 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 10.3 KB | Display | ![]() |
Images | ![]() | 216.9 KB | ||
Filedesc metadata | ![]() | 6.6 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 408.1 KB | Display | ![]() |
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Full document | ![]() | 407.6 KB | Display | |
Data in XML | ![]() | 11.6 KB | Display | |
Data in CIF | ![]() | 15.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6vbwMC ![]() 6v9pC ![]() 6v9qC M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Cascade-TniQ-dsDNA ternary complex | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.064 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
+Entire : Cascade-TniQ-dsDNA ternary complex
+Supramolecule #1: Cascade-TniQ-dsDNA ternary complex
+Supramolecule #2: DNA/RNA
+Supramolecule #3: Cascade ternary complex
+Macromolecule #1: DNA (5'-D(*GP*CP*AP*GP*TP*CP*AP*TP*CP*AP*CP*CP*AP*AP*TP*TP*TP*AP*...
+Macromolecule #2: DNA (100-MER)
+Macromolecule #3: RNA (61-MER)
+Macromolecule #4: Cas8
+Macromolecule #5: Cas7
+Macromolecule #6: Cas6
+Macromolecule #7: TniQ
+Macromolecule #8: ZINC ION
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 0.5 mg/mL |
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Buffer | pH: 7.5 / Component - Formula: Tris / Details: 20 mM HEPES, pH 7.5, 150 mM NaCl, 2 mM DTT |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 2.16 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |