- EMDB-20566: The axonemal doublet microtubule focusing on the I1 dynein extrac... -
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Basic information
Entry
Database: EMDB / ID: EMD-20566
Title
The axonemal doublet microtubule focusing on the I1 dynein extracted from the cryo-electron tomography and subtomographic average of isolated Chlamydomonas wild type cilia
Map data
The I1 dynein of Chlamydomonas WT cilia
Sample
Organelle or cellular component: The I1 dynein averaged from Chlamydomonas wild type cilia
Biological species
Chlamydomonas reinhardtii (plant)
Method
subtomogram averaging / cryo EM / Resolution: 38.0 Å
National Institutes of Health/National Institute of General Medical Sciences
R01GM083122
United States
National Institutes of Health/National Institute of General Medical Sciences
R01GM112050
United States
Citation
Journal: FASEB J / Year: 2021 Title: Structural organization of the intermediate and light chain complex of Chlamydomonas ciliary I1 dynein. Authors: Gang Fu / Chasity Scarbrough / Kangkang Song / Nhan Phan / Maureen Wirschell / Daniela Nicastro / Abstract: Axonemal I1 dynein (dynein f) is the largest inner dynein arm in cilia and a key regulator of ciliary beating. It consists of two dynein heavy chains, and an intermediate chain/light chain (ICLC) ...Axonemal I1 dynein (dynein f) is the largest inner dynein arm in cilia and a key regulator of ciliary beating. It consists of two dynein heavy chains, and an intermediate chain/light chain (ICLC) complex. However, the structural organization of the nine ICLC subunits remains largely unknown. Here, we used biochemical and genetic approaches, and cryo-electron tomography imaging in Chlamydomonas to dissect the molecular architecture of the I1 dynein ICLC complex. Using a strain expressing SNAP-tagged IC140, tomography revealed the location of the IC140 N-terminus at the proximal apex of the ICLC structure. Mass spectrometry of a tctex2b mutant showed that TCTEX2B dynein light chain is required for the stable assembly of TCTEX1 and inner dynein arm interacting proteins IC97 and FAP120. The structural defects observed in tctex2b located these 4 subunits in the center and bottom regions of the ICLC structure, which overlaps with the location of the IC138 regulatory subcomplex, which contains IC138, IC97, FAP120, and LC7b. These results reveal the three-dimensional organization of the native ICLC complex and indicate potential protein-protein interactions that are involved in the pathway by which I1 regulates ciliary motility.
History
Deposition
Aug 7, 2019
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Header (metadata) release
Sep 18, 2019
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Map release
Aug 12, 2020
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Update
Jun 2, 2021
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Current status
Jun 2, 2021
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
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