National Institutes of Health/National Institute on Deafness and Other Communication Disorders
米国
引用
ジャーナル: Front Cell Neurosci / 年: 2019 タイトル: 3D Ultrastructure of the Cochlear Outer Hair Cell Lateral Wall Revealed By Electron Tomography. 著者: William Jeffrey Triffo / Hildur Palsdottir / Junha Song / David Gene Morgan / Kent L McDonald / Manfred Auer / Robert M Raphael / 要旨: Outer Hair Cells (OHCs) in the mammalian cochlea display a unique type of voltage-induced mechanical movement termed electromotility, which amplifies auditory signals and contributes to the ...Outer Hair Cells (OHCs) in the mammalian cochlea display a unique type of voltage-induced mechanical movement termed electromotility, which amplifies auditory signals and contributes to the sensitivity and frequency selectivity of mammalian hearing. Electromotility occurs in the OHC lateral wall, but it is not fully understood how the supramolecular architecture of the lateral wall enables this unique form of cellular motility. Employing electron tomography of high-pressure frozen and freeze-substituted OHCs, we visualized the 3D structure and organization of the membrane and cytoskeletal components of the OHC lateral wall. The subsurface cisterna (SSC) is a highly prominent feature, and we report that the SSC membranes and lumen possess hexagonally ordered arrays of particles. We also find the SSC is tightly connected to adjacent actin filaments by short filamentous protein connections. Pillar proteins that join the plasma membrane to the cytoskeleton appear as variable structures considerably thinner than actin filaments and significantly more flexible than actin-SSC links. The structurally rich organization and rigidity of the SSC coupled with apparently weaker mechanical connections between the plasma membrane (PM) and cytoskeleton reveal that the membrane-cytoskeletal architecture of the OHC lateral wall is more complex than previously appreciated. These observations are important for our understanding of OHC mechanics and need to be considered in computational models of OHC electromotility that incorporate subcellular features.
A: 8335.36 Å / B: 8335.36 Å / C: 1831.5001 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Integer*27
Å/pix. X/Y/Z
8.14
8.14
8.14
M x/y/z
1024
1024
225
origin x/y/z
0.000
0.000
0.000
length x/y/z
8335.360
8335.360
1831.500
α/β/γ
90.000
90.000
90.000
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
-225
NC/NR/NS
1024
1024
225
D min/max/mean
0.000
3906.000
1318.308
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添付データ
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試料の構成要素
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全体 : Cochlea
全体
名称: Cochlea
要素
組織: Cochlea
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超分子 #1: Cochlea
超分子
名称: Cochlea / タイプ: tissue / ID: 1 / 親要素: 0
由来(天然)
生物種: Cavia porcellus (哺乳類)
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実験情報
-
構造解析
手法
ネガティブ染色法, クライオ電子顕微鏡法
解析
電子線トモグラフィー法
試料の集合状態
tissue
-
試料調製
緩衝液
pH: 7
染色
タイプ: POSITIVE / 材質: Uranyl Acetate
糖包埋
材質: Epon
グリッド
詳細: unspecified
凍結
凍結剤: NITROGEN
加圧凍結法
装置: OTHER 詳細: The value given for _emd_high_pressure_freezing.instrument is BALTEC. This is not in a list of allowed values set(['LEICA EM PACT2', 'LEICA EM PACT', 'EMS-002 RAPID IMMERSION FREEZER', ...詳細: The value given for _emd_high_pressure_freezing.instrument is BALTEC. This is not in a list of allowed values set(['LEICA EM PACT2', 'LEICA EM PACT', 'EMS-002 RAPID IMMERSION FREEZER', 'OTHER', 'LEICA EM HPM100', 'BAL-TEC HPM 010']) so OTHER is written into the XML file.
切片作成
ウルトラミクロトーム - 装置: Leica EM UC6 / ウルトラミクロトーム - 温度: 298 K / ウルトラミクロトーム - 最終 厚さ: 100 nm
位置合わせマーカー
Manufacturer: Electron Microscopy Sciences / 直径: 5 nm
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電子顕微鏡法
顕微鏡
FEI/PHILIPS CM200FEG
撮影
フィルム・検出器のモデル: TVIPS TEMCAM-F216 (2k x 2k) 平均電子線量: 10.0 e/Å2