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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-2045 | |||||||||
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Title | Subtomogram average of type VI secretion system (T6SS) sheath | |||||||||
![]() | electron cryotomography, sub-tomogram average | |||||||||
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![]() | type VI secretion system / T6SS / bacterial secretion / sheath / VipA / VipB / VipAB | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | subtomogram averaging / cryo EM | |||||||||
![]() | Basler M / Pilhofer M / Henderson GP / Jensen GJ / Mekalanos JJ | |||||||||
![]() | ![]() Title: Type VI secretion requires a dynamic contractile phage tail-like structure. Authors: M Basler / M Pilhofer / G P Henderson / G J Jensen / J J Mekalanos / ![]() Abstract: Type VI secretion systems are bacterial virulence-associated nanomachines composed of proteins that are evolutionarily related to components of bacteriophage tails. Here we show that protein ...Type VI secretion systems are bacterial virulence-associated nanomachines composed of proteins that are evolutionarily related to components of bacteriophage tails. Here we show that protein secretion by the type VI secretion system of Vibrio cholerae requires the action of a dynamic intracellular tubular structure that is structurally and functionally homologous to contractile phage tail sheath. Time-lapse fluorescence light microscopy reveals that sheaths of the type VI secretion system cycle between assembly, quick contraction, disassembly and re-assembly. Whole-cell electron cryotomography further shows that the sheaths appear as long tubular structures in either extended or contracted conformations that are connected to the inner membrane by a distinct basal structure. These data support a model in which the contraction of the type VI secretion system sheath provides the energy needed to translocate proteins out of effector cells and into adjacent target cells. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 1.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 7.7 KB 7.7 KB | Display Display | ![]() |
Images | ![]() | 319.5 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 229.2 KB | Display | ![]() |
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Full document | ![]() | 228.3 KB | Display | |
Data in XML | ![]() | 4.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
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Annotation | electron cryotomography, sub-tomogram average | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 6.3 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Type VI Secretion System sheath
Entire | Name: Type VI Secretion System sheath |
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Components |
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-Supramolecule #1000: Type VI Secretion System sheath
Supramolecule | Name: Type VI Secretion System sheath / type: sample / ID: 1000 / Number unique components: 1 |
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-Supramolecule #1: Type VI Secretion Sheath
Supramolecule | Name: Type VI Secretion Sheath / type: organelle_or_cellular_component / ID: 1 / Recombinant expression: No / Database: NCBI |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | subtomogram averaging |
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Sample preparation
Buffer | pH: 7.4 / Details: 50mM Tris, 150mM NaCl |
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Grid | Details: R 2/2 Quantifoil |
Vitrification | Cryogen name: OTHER / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK II |
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Electron microscopy
Microscope | FEI POLARA 300 |
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Specialist optics | Energy filter - Name: GIF Gatan |
Date | May 30, 2011 |
Image recording | Category: CCD / Film or detector model: GATAN ULTRASCAN 1000 (2k x 2k) |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal magnification: 34000 |
Sample stage | Specimen holder model: GATAN LIQUID NITROGEN / Tilt series - Axis1 - Min angle: -60 ° / Tilt series - Axis1 - Max angle: 60 ° |
Experimental equipment | ![]() Model: Tecnai Polara / Image courtesy: FEI Company |
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Image processing
Details | Average number of tilts used in the 3D reconstructions: 120. Average tomographic tilt angle increment: 2. |
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Final reconstruction | Algorithm: OTHER / Software - Name: ETOMO,RAPTOR |