National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01GM071940
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM007185
United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI052348
United States
National Institutes of Health/Office of the Director
S10OD018111
United States
National Institutes of Health/National Center for Research Resources (NIH/NCRR)
S10RR23057
United States
National Science Foundation (NSF, United States)
DMR-1548924
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
U24GM116792
United States
Swiss National Science Foundation
P300PA_174358
Switzerland
National Science Foundation (NSF, United States)
DBI-1338135
United States
Citation
Journal: Elife / Year: 2019 Title: Cryo electron tomography with volta phase plate reveals novel structural foundations of the 96-nm axonemal repeat in the pathogen . Authors: Simon Imhof / Jiayan Zhang / Hui Wang / Khanh Huy Bui / Hoangkim Nguyen / Ivo Atanasov / Wong H Hui / Shun Kai Yang / Z Hong Zhou / Kent L Hill / Abstract: The 96-nm axonemal repeat includes dynein motors and accessory structures as the foundation for motility of eukaryotic flagella and cilia. However, high-resolution 3D axoneme structures are ...The 96-nm axonemal repeat includes dynein motors and accessory structures as the foundation for motility of eukaryotic flagella and cilia. However, high-resolution 3D axoneme structures are unavailable for organisms among the Excavates, which include pathogens of medical and economic importance. Here we report cryo electron tomography structures of the 96-nm repeat from , a protozoan parasite in the Excavate lineage that causes African trypanosomiasis. We examined bloodstream and procyclic life cycle stages, and a knockdown lacking DRC11/CMF22 of the nexin dynein regulatory complex (NDRC). Sub-tomogram averaging yields a resolution of 21.8 Å for the 96-nm repeat. We discovered several lineage-specific structures, including novel inter-doublet linkages and microtubule inner proteins (MIPs). We establish that DRC11/CMF22 is required for the NDRC proximal lobe that binds the adjacent doublet microtubule. We propose that lineage-specific elaboration of axoneme structure in reflects adaptations to support unique motility needs in diverse host environments.
History
Deposition
Mar 20, 2019
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Header (metadata) release
May 15, 2019
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Map release
Dec 11, 2019
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Update
Dec 25, 2019
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Current status
Dec 25, 2019
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
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