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- EMDB-19941: Structure of a B-state intermediate committed to discard (Bd-I state) -

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Basic information

Entry
Database: EMDB / ID: EMD-19941
TitleStructure of a B-state intermediate committed to discard (Bd-I state)
Map data
Sample
  • Complex: Bd-I complex
    • RNA: x 4 types
    • Protein or peptide: x 32 types
  • Ligand: x 5 types
KeywordsHelicase / G-patch protein / discard pathway / SPLICING
Function / homology
Function and homology information


nucleolar peripheral inclusion body / mRNA Splicing - Major Pathway / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / spliceosomal complex disassembly / spliceosomal conformational changes to generate catalytic conformation / U2-type post-mRNA release spliceosomal complex / siRNA-mediated pericentric heterochromatin formation / post-mRNA release spliceosomal complex ...nucleolar peripheral inclusion body / mRNA Splicing - Major Pathway / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / spliceosomal complex disassembly / spliceosomal conformational changes to generate catalytic conformation / U2-type post-mRNA release spliceosomal complex / siRNA-mediated pericentric heterochromatin formation / post-mRNA release spliceosomal complex / generation of catalytic spliceosome for first transesterification step / pICln-Sm protein complex / U2-type catalytic step 1 spliceosome / pre-mRNA binding / snRNP binding / spindle pole body / regulatory ncRNA-mediated gene silencing / SMN-Sm protein complex / spliceosomal tri-snRNP complex / U2-type spliceosomal complex / commitment complex / mRNA cis splicing, via spliceosome / U2-type catalytic step 2 spliceosome / U4 snRNP / U2 snRNP / U1 snRNP / U2-type prespliceosome / precatalytic spliceosome / spliceosomal complex assembly / mRNA 5'-splice site recognition / spliceosomal tri-snRNP complex assembly / Prp19 complex / protein K63-linked ubiquitination / U5 snRNA binding / U5 snRNP / U2 snRNA binding / U6 snRNA binding / pre-mRNA intronic binding / spliceosomal snRNP assembly / pericentric heterochromatin / U1 snRNA binding / U4/U6 x U5 tri-snRNP complex / catalytic step 2 spliceosome / peptidyl-prolyl cis-trans isomerase activity / RNA polymerase II CTD heptapeptide repeat P3 isomerase activity / RNA polymerase II CTD heptapeptide repeat P6 isomerase activity / peptidylprolyl isomerase / spliceosomal complex / RING-type E3 ubiquitin transferase / mRNA splicing, via spliceosome / metallopeptidase activity / ubiquitin-protein transferase activity / ubiquitin protein ligase activity / nuclear envelope / protein folding / protein-macromolecule adaptor activity / molecular adaptor activity / cysteine-type deubiquitinase activity / RNA helicase activity / RNA helicase / DNA repair / GTPase activity / mRNA binding / GTP binding / ATP hydrolysis activity / DNA binding / RNA binding / zinc ion binding / ATP binding / nucleus / cytosol / cytoplasm
Similarity search - Function
G patch domain-containing protein, N-terminal / Protein of unknown function (DUF1604) / GCF, C-terminal / GC-rich sequence DNA-binding factor-like protein / TFP11/STIP/Ntr1 / cwf21 / Slt11, RNA recognition motif / Pre-mRNA-splicing factor Cwc2, RNA recognition motif / Pre-mRNA-splicing factor SPF27 / Torus domain ...G patch domain-containing protein, N-terminal / Protein of unknown function (DUF1604) / GCF, C-terminal / GC-rich sequence DNA-binding factor-like protein / TFP11/STIP/Ntr1 / cwf21 / Slt11, RNA recognition motif / Pre-mRNA-splicing factor Cwc2, RNA recognition motif / Pre-mRNA-splicing factor SPF27 / Torus domain / Breast carcinoma amplified sequence 2 (BCAS2) / Torus domain / CWF11 family / Intron-binding protein aquarius, N-terminal / Intron-binding protein aquarius N-terminal / mRNA splicing factor SYF2 / SYF2 splicing factor / G-patch domain / : / : / mRNA splicing factor Cwf21 domain / cwf21 domain / G-patch domain profile. / G-patch domain / glycine rich nucleic binding domain / Pre-mRNA-processing factor 17 / : / : / Pre-mRNA-splicing factor SYF1 middle HAT repeat / Pre-mRNA-splicing factor 19 / Pre-mRNA-processing factor 19 / Prp19/Pso4-like / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, cyclophilin A-like / : / : / Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat / : / STL11, N-terminal / : / Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat / SKI-interacting protein SKIP, SNW domain / SKI-interacting protein, SKIP / SKIP/SNW domain / Pre-mRNA-splicing factor Cwf15/Cwc15 / Cwf15/Cwc15 cell cycle control protein / WD repeat Prp46/PLRG1-like / Pre-mRNA-splicing factor Cwc2/Slt11 / : / G10 protein / Pre-mRNA-splicing factor BUD31 / Pre-mRNA splicing factor component Cdc5p/Cef1, C-terminal / : / : / pre-mRNA splicing factor component / DNA2/NAM7-like helicase / : / Initiation factor eIF-4 gamma, MA3 / MA3 domain / MI domain profile. / Domain in DAP-5, eIF4G, MA-3 and other proteins. / MIF4G domain / Anaphase-promoting complex subunit 4, WD40 domain / Myb-like DNA-binding domain / Middle domain of eukaryotic initiation factor 4G (eIF4G) / MIF4G-like, type 3 / Small nuclear ribonucleoprotein D1 / U-box domain profile. / Anaphase-promoting complex subunit 4 WD40 domain / Modified RING finger domain / U-box domain / Zinc finger, CCCH-type superfamily / zinc finger / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal / 116kDa U5 small nuclear ribonucleoprotein component, C-terminal / Snu114, GTP-binding domain / 116 kDa U5 small nuclear ribonucleoprotein component N-terminus / Pre-mRNA-splicing factor Syf1-like / : / Helicase associated domain (HA2), ratchet-like / DEAD-box helicase, OB fold / Oligonucleotide/oligosaccharide-binding (OB)-fold / Helicase associated domain (HA2), winged-helix / Helicase-associated domain / Helicase associated domain (HA2) Add an annotation / Small nuclear ribonucleoprotein Sm D3 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein F / Sm-like protein Lsm7/SmG / Like-Sm (LSM) domain containing protein, LSm4/SmD1/SmD3 / Sm-like protein Lsm6/SmF / Myb-type HTH DNA-binding domain profile. / Zinc finger, CCCH-type / Zinc finger C3H1-type profile. / LSM domain / LSM domain, eukaryotic/archaea-type / snRNP Sm proteins / HAT (Half-A-TPR) repeat / HAT (Half-A-TPR) repeats
Similarity search - Domain/homology
Pre-mRNA-splicing factor prp5 / Pre-mRNA-processing factor 19 / Small nuclear ribonucleoprotein Sm D2 / Pre-mRNA-splicing factor cwf21 / Pre-mRNA-splicing factor spp42 / Small nuclear ribonucleoprotein Sm D1 / Pre-mRNA-processing factor 17 / Pre-mRNA-splicing factor syf2 / Small nuclear ribonucleoprotein F / Pre-mRNA-splicing factor cwf5 ...Pre-mRNA-splicing factor prp5 / Pre-mRNA-processing factor 19 / Small nuclear ribonucleoprotein Sm D2 / Pre-mRNA-splicing factor cwf21 / Pre-mRNA-splicing factor spp42 / Small nuclear ribonucleoprotein Sm D1 / Pre-mRNA-processing factor 17 / Pre-mRNA-splicing factor syf2 / Small nuclear ribonucleoprotein F / Pre-mRNA-splicing factor cwf5 / Pre-mRNA-splicing factor cwf14 / Small nuclear ribonucleoprotein G / Pre-mRNA-splicing factor cwf10 / Pre-mRNA-splicing factor cwf11 / Pre-mRNA-splicing factor cwf17 / Pre-mRNA-splicing factor cdc5 / Pre-mRNA-splicing factor cwf15 / Peptidyl-prolyl cis-trans isomerase ppi1 / Pre-mRNA-splicing factor cwf2 / Pre-mRNA-splicing factor cwf4 / Pre-mRNA-processing protein 45 / Small nuclear ribonucleoprotein-associated protein B / Putative pre-mRNA-splicing factor ATP-dependent RNA helicase C20H4.09 / Uncharacterized protein C20H4.06c / Pre-mRNA-splicing factor cwf22 / Pre-mRNA-splicing factor cwf3 / Pre-mRNA-splicing factor cwf7 / Small nuclear ribonucleoprotein E / G-patch domain-containing protein C1486.03 / Small nuclear ribonucleoprotein Sm D3
Similarity search - Component
Biological speciesSchizosaccharomyces pombe (fission yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsSoni K / Wild K / Sinning I
Funding support Germany, 2 items
OrganizationGrant numberCountry
German Research Foundation (DFG)SI 586/6-1 Germany
German Research Foundation (DFG)Project-ID 439669440, TP B03 Germany
CitationJournal: Nat Struct Mol Biol / Year: 2025
Title: Structures of aberrant spliceosome intermediates on their way to disassembly.
Authors: Komal Soni / Attila Horvath / Olexandr Dybkov / Merlin Schwan / Sasanan Trakansuebkul / Dirk Flemming / Klemens Wild / Henning Urlaub / Tamás Fischer / Irmgard Sinning /
Abstract: Intron removal during pre-mRNA splicing is of extraordinary complexity and its disruption causes a vast number of genetic diseases in humans. While key steps of the canonical spliceosome cycle have ...Intron removal during pre-mRNA splicing is of extraordinary complexity and its disruption causes a vast number of genetic diseases in humans. While key steps of the canonical spliceosome cycle have been revealed by combined structure-function analyses, structural information on an aberrant spliceosome committed to premature disassembly is not available. Here, we report two cryo-electron microscopy structures of post-B spliceosome intermediates from Schizosaccharomyces pombe primed for disassembly. We identify the DEAH-box helicase-G-patch protein pair (Gih35-Gpl1, homologous to human DHX35-GPATCH1) and show how it maintains catalytic dormancy. In both structures, Gpl1 recognizes a remodeled active site introduced by an overstabilization of the U5 loop I interaction with the 5' exon leading to a single-nucleotide insertion at the 5' splice site. Remodeling is communicated to the spliceosome surface and the Ntr1 complex that mediates disassembly is recruited. Our data pave the way for a targeted analysis of splicing quality control.
History
DepositionMar 26, 2024-
Header (metadata) releaseDec 25, 2024-
Map releaseDec 25, 2024-
UpdateMay 28, 2025-
Current statusMay 28, 2025Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_19941.png

Downloads & links

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Map

FileDownload / File: emd_19941.map.gz / Format: CCP4 / Size: 669.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

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Size
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AxesZ (Sec.)Y (Row.)X (Col.)
0.82 Å/pix.
x 560 pix.
= 460.32 Å		0.82 Å/pix.
x 560 pix.
= 460.32 Å		0.82 Å/pix.
x 560 pix.
= 460.32 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.822 Å
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Histogram (log scale)
Contour LevelBy AUTHOR: 3.55
Minimum - Maximum-20.085021999999999 - 37.729336000000004
Average (Standard dev.)0.00006598179 (±0.9024394)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions560560560
Spacing560560560
CellA=B=C: 460.32 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_19941_msk_1.map
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Half map: #2

Fileemd_19941_half_map_1.map
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Half map: #1

Fileemd_19941_half_map_2.map
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Sample components

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Entire : Bd-I complex

EntireName: Bd-I complex
Components
  • Complex: Bd-I complex
    • RNA: pre-mRNA
    • RNA: U2snRNA
    • RNA: U5snRNA
    • RNA: U6snRNA
    • Protein or peptide: Pre-mRNA-splicing factor spp42
    • Protein or peptide: Pre-mRNA-splicing factor cwf10
    • Protein or peptide: Pre-mRNA-splicing factor cwf17
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
    • Protein or peptide: Small nuclear ribonucleoprotein-associated protein B
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D1
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D2
    • Protein or peptide: Small nuclear ribonucleoprotein E
    • Protein or peptide: Small nuclear ribonucleoprotein F
    • Protein or peptide: Small nuclear ribonucleoprotein G
    • Protein or peptide: Pre-mRNA-splicing factor prp5
    • Protein or peptide: Pre-mRNA-processing protein 45
    • Protein or peptide: Pre-mRNA-splicing factor cwf5
    • Protein or peptide: Pre-mRNA-splicing factor cwf11
    • Protein or peptide: Pre-mRNA-splicing factor cwf14
    • Protein or peptide: Pre-mRNA-splicing factor cwf2
    • Protein or peptide: Pre-mRNA-splicing factor cwf15
    • Protein or peptide: Pre-mRNA-splicing factor cwf4
    • Protein or peptide: Pre-mRNA-processing factor 19
    • Protein or peptide: Pre-mRNA-splicing factor cdc5
    • Protein or peptide: Pre-mRNA-splicing factor cwf3
    • Protein or peptide: Pre-mRNA-splicing factor syf2
    • Protein or peptide: Pre-mRNA-splicing factor cwf7
    • Protein or peptide: Pre-mRNA-processing factor 17
    • Protein or peptide: Pre-mRNA-splicing factor cwf21
    • Protein or peptide: Pre-mRNA-splicing factor cwf22
    • Protein or peptide: Peptidyl-prolyl cis-trans isomerase ppi1
    • Protein or peptide: UNK1
    • Protein or peptide: G-patch domain-containing protein C1486.03
    • Protein or peptide: UNK2
    • Protein or peptide: Uncharacterized protein C20H4.06c
    • Protein or peptide: Putative pre-mRNA-splicing factor ATP-dependent RNA helicase C20H4.09
  • Ligand: MAGNESIUM ION
  • Ligand: POTASSIUM ION
  • Ligand: INOSITOL HEXAKISPHOSPHATE
  • Ligand: GUANOSINE-5'-TRIPHOSPHATE
  • Ligand: ZINC ION

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Supramolecule #1: Bd-I complex

SupramoleculeName: Bd-I complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#36
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)

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Macromolecule #1: pre-mRNA

MacromoleculeName: pre-mRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 9.135291 KDa
SequenceString:
UUUUUAUUAA AAAAUGGUAU GUUUUUUUU

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Macromolecule #2: U2snRNA

MacromoleculeName: U2snRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 59.207473 KDa
SequenceString:
AUUCUCUCUU UGCCUUUUGG CUUAGAUCAA GUGUAGUAUC UGUUCUUUUC AGUUUAAUCG CUGAAAUCAC CUCACUGAGG UGUUUCCGA UUAAUCUUGU UUUUGGUUUG AGUUGGAAAG CCUCUGGCUU GCUAUGCUUU CCGACACUGG UGUUCUUGCU A UUGCACUA CUGGCAAGCG ACGCCGAA

GENBANK: GENBANK: X55772.1

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Macromolecule #3: U5snRNA

MacromoleculeName: U5snRNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 38.191363 KDa
SequenceString:
AUAAUCCGUC AAAGCACUUU GCAAAAGCUA ACGUAUCUGU UUCUUGCCUU UUACCAGAAA CAGCCGUUUG UAAGGUGUGC UAAUUUGAC UGUAUAGUUU UUGUAAUCUU UUUCUUGAAA C

GENBANK: GENBANK: X16573.1

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Macromolecule #4: U6snRNA

MacromoleculeName: U6snRNA / type: rna / ID: 4 / Number of copies: 1
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 31.863982 KDa
SequenceString:
GAUCUUCGGA UCACUUUGGU CAAAUUGAAA CGAUACAGAG AAGAUUAGCA UGGCCCCUGC ACAAGGAUGA CACUGCGACA UUGAGAGAA AACCCAUUUU

GENBANK: GENBANK: XM_013166828.1

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Macromolecule #5: Pre-mRNA-splicing factor spp42

MacromoleculeName: Pre-mRNA-splicing factor spp42 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 274.917781 KDa
SequenceString: MASLPPGNPP PPPPPPGFEP PSQPPPPPPP GYVKKRKNKT PAQSGNLEKQ LNERARKWRA SQKSKFGVKR KQGYVQTEKA DLPPEHLRK IMKDRGDMSS RKFRADKRSY LGALKYLPHA VLKLLENMPM PWEEYREVKV LYHVTGAITF VNESPRVIEP H FIAQWGTM ...String:
MASLPPGNPP PPPPPPGFEP PSQPPPPPPP GYVKKRKNKT PAQSGNLEKQ LNERARKWRA SQKSKFGVKR KQGYVQTEKA DLPPEHLRK IMKDRGDMSS RKFRADKRSY LGALKYLPHA VLKLLENMPM PWEEYREVKV LYHVTGAITF VNESPRVIEP H FIAQWGTM WMMMRREKRD RKNFKRLRFP PFDDEEPPFS IDQLLDLEPL EAIRMDLDEE DDAPVMDWFY ENKALEDTPH VN GPTYRRW KLNLPQMANL HRLGYQLLSD LRDDNYFYLF NDNSFFTAKA LNVAIPGGPK FEPLYKDEAP EMEDWNEFND IYK LIIRHP IKTEYRIAFP YLYNSRARSV ALSEYHQPSN VFVPPEDPDL PAFFWDPIIN PITSRQLTLH ELDTSPEDSA IEED PNFEI PFDPFFHSED IEFEHTASAL ILLWAPHPFN KRSGATKRAQ DVPLIKHWYL EHCPPNQPVK VRVSYQKLLK SHVMN KLHM AHPKSHTNRS LLRQLKNTKF FQSTSIDWVE AGLQVCRQGY NMLQLLIHRK GLTYLHLDYN CNLKPTKTLT TKERKK SRF GNAFHLMREI LRLTKLIVDS HVQYRLGNID AYQLADGLHY IFNHVGQLTG MYRYKYRLMR QIRACKDFKH LIYYRFN TG PVGKGPGCGF WAPSWRVWLF FLRGIVPLLE RWLGNLLARQ FEGRHSTGVA KQITKQRVDS HQDLELRAAV MNDILDMI P EGIRQGKSKT ILQHLSEAWR CWKANIPWKV PGLPAPIENM ILRYVKSKAD WWTSVAHFNR ERIRRGATVD KTVAKKNLG RLTRLWLKAE QERQHNYLKD GPYVTADEAV AIYTTFVHWL ESRRFQPIPF PPLSYKHDTK LLVLALERLK EAYSVKGRLN QSQREELAL VEQAYDNPHE MLSQIKRRLL TMRTFKEVGI EFMDMYSHLI PVYSVDPMEK ICDAYLDQYL WFEADRRHLF P SWVKPSDS EPPPLLVYKW CQGINNLTDV WETSNGECNV LMETRLSKVF EKVDLTLLNR LMSLLMDTNL ASYASAKNNV VL SYKDMSH TNSYGLVRGL QFSSFIWQFY GLVLDLLILG LQRATEIAGP ADAPNDFLHF KDQATETSHP IRLYTRYIDK VYI MFRFTD EESRDLIQRF LNENPDPTNS NVVNYSKGKK NCWPRDARMR LMKHDVNLGR AVFWEIRNRL PRSLTTLEWE DTFP SVYSK DNPNLLFSMT GFEVRILPKI RQNEEFSLKD GVWNLTDNRT KQRTAQAFIR VTEDGINQFG NRIRQILMSS GSTTF TKIA NKWNTALIAL MTYYREAAIS TPELLDLLVK CESKIQTRVK ISLNSKMPSR FPPAVFYSPK ELGGLGMLSM GHVLIP QSD LRWSKQTDTG ITHFRSGMTT NGEHLIPNLY RYIQPWESEF IDSQRVWAEY AMKRQEALQQ NRRLTLEDLE DSWDRGI PR INTLFQKDRH TLAYDKGWRV RTEFKQYQLL KNNPFWWTSQ RHDGKLWQLN NYRVDVIQAL GGVEGILEHT MFKATGFP S WEGLFWEKAS GFEESMKFKK LTNAQRSGLN QIPNRRFTLW WSPTINRANV YVGFQVQLDL TGIMMHGKIP TLKISLIQI FRSHLWQKIH ESVVWDLCQV LDQELESLQI ETVQKETIHP RKSYKMNSSC ADILLLAAYK WNVSRPSLLN DNRDVLDNTT TNKYWIDVQ LRFGDYDSHD IERYTRAKFL DYSTDAQSMY PSPTGVLIGI DLCYNMHSAY GNWIPGMKPL IQQSMNKIMK A NPALYVLR ERIRKGLQLY ASEPQEQYLS SSNYAELFSN QIQLFVDDTN VYRVTIHKTF EGNLTTKPIN GAIFIFNPRT GQ LFLKVIH TSVWAGQKRL GQLAKWKTAE EVAALIRSLP VEEQPRQIIV TRKGMLDPLE VHLLDFPNIT IKGSELQLPF QAI IKLDKI NDLILRATEP QMVLFNLYDD WLQSVSSYTA FSRLILILRA LNVNTEKTKL ILRPDKSIIT KENHVWPNLD DQQW LDVEP KLRDLILADY AKKNNINVAS LTNSEVRDII LGMTITAPSL QRQQIAEIEK QGRENAQVTA VTTKTTNVHG DEMVV TTTS AYENEKFSSK TEWRNRAISS ISLPLRTKNI YVNSDNISET FPYTYILPQN LLRKFVTISD LRTQVAGYMY GKSPSD NPQ IKEIRCIALV PQLGSIRNVQ LPSKLPHDLQ PSILEDLEPL GWIHTQSSEL PYLSSVDVTT HAKILSSHPE WDTKAVT LT VSYIPGSISL AAYTVSKEGI EWGSKNMDIN SDEAIGYEPS MAEKCQLLLS DRIQGFFLVP EEGVWNYNFN GASFSPKM T YSLKLDVPLP FFALEHRPTH VISYTELETN DRLEEDMPDA FA

UniProtKB: Pre-mRNA-splicing factor spp42

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Macromolecule #6: Pre-mRNA-splicing factor cwf10

MacromoleculeName: Pre-mRNA-splicing factor cwf10 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 111.445281 KDa
SequenceString: MMEEDLYDEF GNYIGPENEE DEEELFPQAP SPTIAQVPSF EEVIPDEELE DVERAEEMAL SHLEPQNAVV LHEDKQYYPS AEEVYGSNV DIMVQEQDTQ PLSQPIIEPI RHKRIAIETT NVPDTVYKKE FLFGLLTGTD DVRSFIVAGH LHHGKSALLD L LVYYTHPD ...String:
MMEEDLYDEF GNYIGPENEE DEEELFPQAP SPTIAQVPSF EEVIPDEELE DVERAEEMAL SHLEPQNAVV LHEDKQYYPS AEEVYGSNV DIMVQEQDTQ PLSQPIIEPI RHKRIAIETT NVPDTVYKKE FLFGLLTGTD DVRSFIVAGH LHHGKSALLD L LVYYTHPD TKPPKRRSLR YTDTHYLERE RVMSIKSTPL TLAVSDMKGK TFAFQCIDTP GHVDFVDEVA APMAISDGVV LV VDVIEGV MINTTRIIKH AILHDMPIVL VLNKVDRLIL ELRLPPNDAY HKLRHVIDEV NDNICQISKD LKYRVSPELG NVC FASCDL GYCFTLSSFA KLYIDRHGGI DVDLFSKRLW GDIYFDSKTR KFAKQSLDGS GVRSFVHFIL EPLYKLHTLT ISDE AEKLK KHLSSFQIYL KPKDYLLDPK PLLQLICASF FGFPVGFVNA VTRHIPSPRE NAARKASQSY IGPINSSIGK AILEM SREE SAPLVMHVTK LYNTVDANNF YAFARVYSGQ VKKGQKVKVL GENYSLEDEE DMVVAHIAEI CVPCARYRLH VDGAVA GML VLLGGVDNSI SKTATIVSDN LKDDPYIFRP IAHMSESVFK VAVEPHNPSE LPKLLDGLRK TNKSYPLSIT KVEESGE HT IFGTGEMYMD CLLYDLRTLY SEIEIRVSDP VARFCETAVD TSSIKCFSDT PNKKNRITMV VEPLEKGISN DIENGKVN I NWPQKRISEF FQKNYDWDLL ASRSIWAFGP DDRGTNILRD DTLSTDVDKN VLNSVKEYIK QGFQWGTREG PLCDETIRN VNFRLMDVVL APEQIYRGGG QIIPTARRVC YSSFLTASPR LMEPVYMVEV HAPADSLPII YDLLTRRRGH VLQDIPRPGS PLYLVRALI PVIDSCGFET DLRVHTQGQA MCQMVFDHWQ VVPGDPLDKS IKPKPLEPAR GSDLARDFLI KTRRRKGLVE D VSTTRYFD QEMIDSLKEA GVVLSL

UniProtKB: Pre-mRNA-splicing factor cwf10

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Macromolecule #7: Pre-mRNA-splicing factor cwf17

MacromoleculeName: Pre-mRNA-splicing factor cwf17 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 37.477242 KDa
SequenceString: MDKRKESESA TNGHLVKRIR IQDSSLITEG SVLQRTSDLN VPNLQMFGHT AEVLVARFDP SGSYFASGGM DRQILLWNVF GDVKNYGVL NGCKGAITDL QWSRDSRVVY CSSSDTHLMS WDAVSGQKIR KHKGHAGVVN ALDVLKVGSE LLTSVSDDCT M KVWDSRSK ...String:
MDKRKESESA TNGHLVKRIR IQDSSLITEG SVLQRTSDLN VPNLQMFGHT AEVLVARFDP SGSYFASGGM DRQILLWNVF GDVKNYGVL NGCKGAITDL QWSRDSRVVY CSSSDTHLMS WDAVSGQKIR KHKGHAGVVN ALDVLKVGSE LLTSVSDDCT M KVWDSRSK DCIKTIEEKY PLTAVAIAQQ GTQVFIGGID GAIKIWDLRN NHCSHVLKGH KDIITSLAIS KDGSSLLSNS MD NTVRIFD VKPFASAQRQ LQIFEGAIHG QEHNLLGVAW SRNSRFVGAG SSDKNVYVWS ATGDLRYVLP GHEGSVNHVD FHP HQDIIL SCSSDRTIFL GELN

UniProtKB: Pre-mRNA-splicing factor cwf17

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Macromolecule #8: Small nuclear ribonucleoprotein Sm D3

MacromoleculeName: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 11.050884 KDa
SequenceString:
MSLCIKLLHE TQGHIVTMEL ENGSTYRGKL IEAEDNMNCQ MRDISVTARD GRVSHLDQVY IRGSHIRFLI VPDMLRNAPM FKVGPGRSV PLPTRGRR

UniProtKB: Small nuclear ribonucleoprotein Sm D3

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Macromolecule #9: Small nuclear ribonucleoprotein-associated protein B

MacromoleculeName: Small nuclear ribonucleoprotein-associated protein B / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 15.493004 KDa
SequenceString:
MGTTKMVSLL NHSLNVTTKD GRTFVGQLLA FDGFMNLVLS DCQEYRHIKK QNVPSNSVYE EKRMLGLVIL RGEFIVSLSV QGPPPMDPS MRGSLLSGPG VARPAGRGIP LGQAPVGLAG PVRGVGYTAP PPPAGFGRGA PPPGFRPV

UniProtKB: Small nuclear ribonucleoprotein-associated protein B

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Macromolecule #10: Small nuclear ribonucleoprotein Sm D1

MacromoleculeName: Small nuclear ribonucleoprotein Sm D1 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 13.115422 KDa
SequenceString:
MKLVRFLMKL TNETVSIELK NGTIVHGTIT SVDMQMNTHL KAVKMTVKGR EPVPVETLSI RGNNIRYYIL PDSLPLDTLL IDDSTKPKQ KKKEVVRGRG RGRGRGTRGR GRGASRGF

UniProtKB: Small nuclear ribonucleoprotein Sm D1

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Macromolecule #11: Small nuclear ribonucleoprotein Sm D2

MacromoleculeName: Small nuclear ribonucleoprotein Sm D2 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 13.119311 KDa
SequenceString:
MADLVDKPRS ELSEIELARL EEYEFSAGPL SVLQQAVKNH DQVLINCRNN KKLLARVKAF DRHSNMVLEN VKEMWTEKKR TASGKKGKA INKDRFISKM FLRGDGVVLV VRIPSA

UniProtKB: Small nuclear ribonucleoprotein Sm D2

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Macromolecule #12: Small nuclear ribonucleoprotein E

MacromoleculeName: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 9.702353 KDa
SequenceString:
MSGRVQKVMI PPINFIFKLL QQHTPVSIWL FEQTDIRLQG QIRGFDEFMN IVLDDAVQVD AKNNKRELGR ILLKGDNITL IQAI

UniProtKB: Small nuclear ribonucleoprotein E

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Macromolecule #13: Small nuclear ribonucleoprotein F

MacromoleculeName: Small nuclear ribonucleoprotein F / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 8.667062 KDa
SequenceString:
MSFVPVNPKP FLQGLIGKPV LVRLKWGQEY KGTLQSVDSY MNLQLLNAEE LVDGVKTGDL GEILIRCNNV LWVGESTV

UniProtKB: Small nuclear ribonucleoprotein F

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Macromolecule #14: Small nuclear ribonucleoprotein G

MacromoleculeName: Small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 8.616038 KDa
SequenceString:
MSKAGAPDLK KYLDRQVFVQ LNGSRKVYGV LRGYDIFLNI VLEDSIEEKV DGEKVKIGSV AIRGNSVIMI ETLDKMT

UniProtKB: Small nuclear ribonucleoprotein G

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Macromolecule #15: Pre-mRNA-splicing factor prp5

MacromoleculeName: Pre-mRNA-splicing factor prp5 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 52.492133 KDa
SequenceString: MTEAKNISDD TDVLSLTTNS LRTSKTLFGA EFGSVTSFDD TVAQNLKRTY KEHLEYGSVL GGTVGKRKNR HYEEDTIGSN ALTVRADSE NPSSQVITKF SDPNKKIAGQ VSMQSLEKIK GVPEAAHRIA GESQASLVKR TLAEQIRPEW HAPWTLMRVI S GHLGWVRC ...String:
MTEAKNISDD TDVLSLTTNS LRTSKTLFGA EFGSVTSFDD TVAQNLKRTY KEHLEYGSVL GGTVGKRKNR HYEEDTIGSN ALTVRADSE NPSSQVITKF SDPNKKIAGQ VSMQSLEKIK GVPEAAHRIA GESQASLVKR TLAEQIRPEW HAPWTLMRVI S GHLGWVRC VDVEPGNQWF CTGAGDRTIK IWDLASGVLK LTLTGHIATV RGLAVSPRHP YLFSCGEDKM VKCWDLETNK VI RHYHGHL SGVYALKLHP TLDVLVTAGR DAVARVWDMR TRQNVHVLSG HKSTVASLAV QEFDPQVVTG SMDSTIRLWD LAA GKTLTT LTHHKKTVRA LSLHPDEFTF ASGSSDNIKH WKFPEGAFMG NFEGHNAIVN TLSINSDNVM FSGADNGSMC FWDW KSGHK YQELQSVVQP GSLDSEAGIF ASSFDKTGLR LITCEADKSV KIYKQVDNAT PETHPNLPWT PSNLRRRY

UniProtKB: Pre-mRNA-splicing factor prp5

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Macromolecule #16: Pre-mRNA-processing protein 45

MacromoleculeName: Pre-mRNA-processing protein 45 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 62.798176 KDa
SequenceString: MALLSEELSS ILPNPDFDDE EEDYVERETS HADERQIGVK FHIPPYGQRK GWFPSSPEDF GDGGAFPEIH VAQYPLDMGR KRSAKSAGN TLALQVTSSG AVDYNAIARQ GHEHGELVQA SFRDLIPLRA RLGVGEISLE KPSDEQKQEV ANKTKLALQK I LSKQIAQS ...String:
MALLSEELSS ILPNPDFDDE EEDYVERETS HADERQIGVK FHIPPYGQRK GWFPSSPEDF GDGGAFPEIH VAQYPLDMGR KRSAKSAGN TLALQVTSSG AVDYNAIARQ GHEHGELVQA SFRDLIPLRA RLGVGEISLE KPSDEQKQEV ANKTKLALQK I LSKQIAQS QPKSAVVQQR DDPVYIRYTP SNQMGQALSK QRIIKMVTAE QDPMEPPKFR HKKVPRGPPS PPPPVLHSPP RK VSAQEQQ DWQIPPSISN WKNPKGYTIP LDKRLAADGR GLNDVEINDG FAKFSEALYT VERQAREEVR YRAIMRQKMA EKE KQEKEQ RLFMLAQKAR EDRMGRNAAS SGPSHAKPRS TSVSSEERSR SRAGSFSHHS ESENEDEDSE AFRRRQELRR ERRR QAEKD LRLSRMGAEK RAKLAEKDRP RDVAERVALG LSKPSMSSDT MIDSRLFNQA SGLGSGFQDE DSYNVYDKPW RAAPS STLY RPGATLSRQV DASAELERIT SESRYDVLGN AHKKFKGSDE VVESRAGPVT FEKDIADPFG VDTFLNNVSS KKT

UniProtKB: Pre-mRNA-processing protein 45

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Macromolecule #17: Pre-mRNA-splicing factor cwf5

MacromoleculeName: Pre-mRNA-splicing factor cwf5 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 39.640363 KDa
SequenceString: MSHGPKGDIN KQEWEDVEFP SICERCLGDN SYVRMTKEPL GQECKICSKP CTIFRWIKER GQKPGKTQIC VGCARYKNCC QSCMLDLQF GLPIALRDAA LKLVESGPTN DINREFFAQN QQRLLSNGET AYDSQEASAA ARNLVKKVEK RELHSRPPKR K LDDVESKQ ...String:
MSHGPKGDIN KQEWEDVEFP SICERCLGDN SYVRMTKEPL GQECKICSKP CTIFRWIKER GQKPGKTQIC VGCARYKNCC QSCMLDLQF GLPIALRDAA LKLVESGPTN DINREFFAQN QQRLLSNGET AYDSQEASAA ARNLVKKVEK RELHSRPPKR K LDDVESKQ ILKEARASDA SLNAERPLFP VKKIINGNVS LSINMEPPKD KKIASLFLIG VEDELADYKI RKHFEQYGPL KS VVCSHRA KCAFVNFKTR SSAEIAAAAS PDGNVVIEGF RLKVQWGKPR SLGGPEGEVR NAKLADLVMR GSSHGNKTSQ KST IKNIEN EDHENTKSPA VAIPIDPNQP RYRSQIPR

UniProtKB: Pre-mRNA-splicing factor cwf5

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Macromolecule #18: Pre-mRNA-splicing factor cwf11

MacromoleculeName: Pre-mRNA-splicing factor cwf11 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 148.532906 KDa
SequenceString: MVLYKNKKKQ IINYANSNWG QNEGVEFQET MLQCILEAVI VSKDAKQVLS LLHELKLFEN FLWQRVNTEM SLNHINLTCM LLLYKSKYE YITWDLIDEN RFQLFFEKVI EVSLSLNLSE VVYMIQFITL CFQFSNIEKL RKLVYQLTNI SILNSLDNLD K VKYLLHDS ...String:
MVLYKNKKKQ IINYANSNWG QNEGVEFQET MLQCILEAVI VSKDAKQVLS LLHELKLFEN FLWQRVNTEM SLNHINLTCM LLLYKSKYE YITWDLIDEN RFQLFFEKVI EVSLSLNLSE VVYMIQFITL CFQFSNIEKL RKLVYQLTNI SILNSLDNLD K VKYLLHDS SSLTKAFDSY KEKRPSIVEK FPLHNLLSRW IHSLLIKSIS YAQTEKQEAK VTPLLAIINM SLVLLSAFPT RR FAHPVIE DSCFYTALRM SLYYDSNELF KKMTDDLNYV LKFPFDNTRG NEYEKEQKIR NDELVYYHLQ LTLFSDFQKE LGD LVFCTQ TSLQQRQKLE EITSFLSFNS LKSLCSKCYL RTSFPEKYAI KVDFEFLKNV FINTYDRTRL VNDYDEIINF TLKD VLGER SVMDQENSLT NYFLLQNTAI QYLSISFFMR QQSKAYKKLL LRSLYAELLN FSEQYRRLSI KNATKNLTKD NFFSL NNFK VTSVAPPQIG QVLPQFVKCQ MGLSRPGPFH SALRDLKNSI KSPFLCLIYI SKDMEYKLLH GNALDPLEGV TDFTIA TIC NDDVGMFQSD MQSDSDNKSI NVYLSPFYYH SLAGLGEYRP KQLKFNFALV LSPEANKYWL DLNILVSLLN RAKEFPK WF EDLFLGFGTP DICAFPNAGL NSIYARNLFN TVEQLQSVLP NCHVPSNLST ESLLIKFYTN QNKISADVTA SDRHFLLP S NRLYTYNDKQ LESILRGSQP GLTMVNGPTR CGKHVLVCKL LEVLQDTSPN DRTVVLSDSN FSMNTLFTLL EKARCFHQG HLLYLSDEGK DETLERYGTL SSWISKLPGL LREIGRLAAS IQAPGSHDAS PDTALYFRDA YIKRLWEKYL NTVDDKDSVD AYNRFPFHS YFGDKSKRPI ETYNKDNFFD YATKLYGELE YMFQQLEEIR PFGLLRYYED QELYALCQQS RIIGCTWTSL S TRLGTLKE KGFCFNNLIV MNSQNISESS ITSILLSNCE PTGFDRLVLL GNQYLTSGNQ DINNTSNGSL FKRLRYLKSR II DLNTQYN VRESISSLCS SIYPLDIKTV DSSPNKRLDY GNSGFAHEVQ FINVGAFKGS QETEPVSGYK QNLGEAEYAV ALF QYMRML GYPTNEIVIC TLYESQVSLL NEIISVRCSH NSFFGQPAFV GTVEKLPSDK RVNFVIFTTV ESKEASDHWN PKTF YKAFS ACSYGLYVLC NRDLFRSTRG LEKLWNEIEK TPDKLLLTTG EIYPSSHKIG SSVETFEIEN LLHLSNYVVE MTKKR LNTN

UniProtKB: Pre-mRNA-splicing factor cwf11

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Macromolecule #19: Pre-mRNA-splicing factor cwf14

MacromoleculeName: Pre-mRNA-splicing factor cwf14 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 17.12285 KDa
SequenceString:
MPRLRTSRTK RPPDGFDEIE PTLIEFQDRM RQIENTMGKG TKTEMLAPIF QLHHQRSRYI YDLYYKREAI STELYNWLLK QNYADGNLI AKWKKPGYEK LCCLRCIQTA ESKFGSTCIC RVPKSKLDKD QRVRCTHCGC NGCASCD

UniProtKB: Pre-mRNA-splicing factor cwf14

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Macromolecule #20: Pre-mRNA-splicing factor cwf2

MacromoleculeName: Pre-mRNA-splicing factor cwf2 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 44.343074 KDa
SequenceString: MSENGLEQEV TVEEKNNDVT EKILVEGEKS KEYEETPRKV KIVKRKKQPA RKQIETRPEY EMEPEQPGQV YNLWYNKWSG GMRQDPLKS QVKSETRCVI SRDSGYTKAD KNPGSFFCLY FARGMCSEGS KCEYLHRLPK DTDFFNANVD CFGREKHADY R DDMGGVGS ...String:
MSENGLEQEV TVEEKNNDVT EKILVEGEKS KEYEETPRKV KIVKRKKQPA RKQIETRPEY EMEPEQPGQV YNLWYNKWSG GMRQDPLKS QVKSETRCVI SRDSGYTKAD KNPGSFFCLY FARGMCSEGS KCEYLHRLPK DTDFFNANVD CFGREKHADY R DDMGGVGS FLRQNYTLYV GGITPTDDIE EIVSRHFAEW GDIERIRVLN SRGIAFITYL NEANAQFAKE AMAHQSLDHD EC LNVRWAT TDPNPASQAR NQRRLEERAA NAVKKLLPKQ FLLDLEETKN GKSGNRKRKL ELEFGLKGYV PSDDLLYADG ANS VHNQLA ANEFPNKSQS EEGSNDDHKS VTTTESQNKF VNSQILSDLQ VAKQAVHTNQ SALVSYYDSD ED

UniProtKB: Pre-mRNA-splicing factor cwf2

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Macromolecule #21: Pre-mRNA-splicing factor cwf15

MacromoleculeName: Pre-mRNA-splicing factor cwf15 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 30.478979 KDa
SequenceString: MTTAHRPQFD PARGHSEMAP TRITSSRALP AHLKLKYRQE SQGTEEEVRK QDLREALLRA EAAHFATQEH GASSEEVSQN SKLIEGFTS PSTDDKPNND VEVDYQELLR QTLEADEDAS DSDDSVDSSN KNSEVSIKRR KTESNSQESV DSSNSESSDE E SDSEDETQ ...String:
MTTAHRPQFD PARGHSEMAP TRITSSRALP AHLKLKYRQE SQGTEEEVRK QDLREALLRA EAAHFATQEH GASSEEVSQN SKLIEGFTS PSTDDKPNND VEVDYQELLR QTLEADEDAS DSDDSVDSSN KNSEVSIKRR KTESNSQESV DSSNSESSDE E SDSEDETQ QLLRELENIK QERKREQMLQ EEKNRALEQE KREREIAFGN ELLNKASSGS FQVKRRWDED VVFRNTHKGV DD TPRPGFV NDMLRSEFHK KFLARFVD

UniProtKB: Pre-mRNA-splicing factor cwf15

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Macromolecule #22: Pre-mRNA-splicing factor cwf4

MacromoleculeName: Pre-mRNA-splicing factor cwf4 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 80.964758 KDa
SequenceString: MTSEAPRVKN KNPAPIQISA EQLLREAVER QDVAFVPPKI NITDLEELQE FQGRKRKEFE DAIRRNRLAM GHWMRYGQWE LDQKEFARA RSVFERALDV DSTYIPLWLK YIECEMKNRN INHARNLFDR AVTQLPRVDK LWYKYVYMEE MLGNITGCRQ V FERWLKWE ...String:
MTSEAPRVKN KNPAPIQISA EQLLREAVER QDVAFVPPKI NITDLEELQE FQGRKRKEFE DAIRRNRLAM GHWMRYGQWE LDQKEFARA RSVFERALDV DSTYIPLWLK YIECEMKNRN INHARNLFDR AVTQLPRVDK LWYKYVYMEE MLGNITGCRQ V FERWLKWE PDENCWMSYI RMERRYHENE RARGIYERFV VVHPEVTNWL RWARFEEECG NAANVRQVYL AAIDALGQEF LN ERFFIAF AKFEIRQKEY ERARTIFKYA IDFMPRSKSM ELYKEYTHFE KQFGDHLGVE STVLDKRRLQ YEKLLKDSPY DYD TWLDLL KLEESAGDIN TIRETYEKAI AKVPEVVEKN AWRRYVYIWL NYCLFEEIDV KDVDRARKVY QEALKLIPHK KFTF AKLWL MYAMFELRQR KIDVARKTLG RALGMCPKPK LFRGYIEFED AIKQFDRCRI LYEKWILYDP EACAPWLGYA ALETK LGDS DRARALYNLA VNQPILETPE LVWKAYIDFE FEEMEYGKAR SIYQQLLRTA PHVKVWISFA NFEIAHLEDD DEEPPN EEV ASPTAVVRAR NVFENALAHL RQQGLKEERV VLLEAWKQFE AMHGTEDTRK HVSSLMPQVV KKRRRLEDGS FEEYLDY LF PDTATDQGDK MRKMLELSRK WKEEMAKKKL EA

UniProtKB: Pre-mRNA-splicing factor cwf4

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Macromolecule #23: Pre-mRNA-processing factor 19

MacromoleculeName: Pre-mRNA-processing factor 19 / type: protein_or_peptide / ID: 23 / Number of copies: 4 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 54.243988 KDa
SequenceString: MFCSISGETP KEPVISRVSG NVYEKRLIEQ VIRETSKDPV TQQECTLEDL VPVKVPDFVR PRPPSATSLP ALLSLFQEEW DSVALEQFE LRRNLTETKQ ELSTALYSLD AALRVISRLT KERDEAREAL AKFSDNIGTV SSKTIEVQEV EMGESDDQLK T SLRSTVEK ...String:
MFCSISGETP KEPVISRVSG NVYEKRLIEQ VIRETSKDPV TQQECTLEDL VPVKVPDFVR PRPPSATSLP ALLSLFQEEW DSVALEQFE LRRNLTETKQ ELSTALYSLD AALRVISRLT KERDEAREAL AKFSDNIGTV SSKTIEVQEV EMGESDDQLK T SLRSTVEK TFQELSSKRK RTKLQPKWAT DDAVSQLLQA TPSTILENLE TESTTSFFPS PENSSFVLCL HKDELLCLDI QS NSTLKIF EGSALACCWL TSSKIAVATA DAISIFEFPV SSSGLQSVGE PTSSIPIDEK VNFLQAHPSG EYLLAASNEK CYI FSLKSQ VYNITVAQHI TSLAVHPDGN LFVAGLENGE LRFFETSSGN ELTKFGPHSS PVKTLQFGEN GYWLVVTTND DSDI FIWDL RKSELVQKIP LQTKVAAVSL DITSQLLVSS DGETLYVHIY VKSSKSWRCM SQTHVSSISN LVWLNELHQL LFSTS NGAI LRLG

UniProtKB: Pre-mRNA-processing factor 19

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Macromolecule #24: Pre-mRNA-splicing factor cdc5

MacromoleculeName: Pre-mRNA-splicing factor cdc5 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 86.981344 KDa
SequenceString: MVVLKGGAWK NTEDEILKAA VSKYGKNQWA RISSLLVRKT PKQCKARWYE WIDPSIKKTE WSREEDEKLL HLAKLLPTQW RTIAPIVGR TATQCLERYQ KLLDDLEAKE NEQLGLISGE GAEAAAPVND PNSRLRFGEA EPNLETLPAL PDAIDMDEDE K EMLSEARA ...String:
MVVLKGGAWK NTEDEILKAA VSKYGKNQWA RISSLLVRKT PKQCKARWYE WIDPSIKKTE WSREEDEKLL HLAKLLPTQW RTIAPIVGR TATQCLERYQ KLLDDLEAKE NEQLGLISGE GAEAAAPVND PNSRLRFGEA EPNLETLPAL PDAIDMDEDE K EMLSEARA RLANTQGKKA KRKDREKQLE LTRRLSHLQK RRELKAAGIN IKLFRRKKNE MDYNASIPFE KKPAIGFYDT SE EDRQNFR EKREADQKII ENGIRNNEME SEGRKFGHFE KPKPIDRVKK PNKDAQEEKM RRLAEAEQMS KRRKLNLPSP TVS QDELDK VVKLGFAGDR ARAMTDTTPD ANYSTNLLGK YTQIERATPL RTPISGELEG REDSVTIEVR NQLMRNREQS SLLG QESIP LQPGGTGYTG VTPSHAANGS ALAAPQATPF RTPRDTFSIN AAAERAGRLA SERENKIRLK ALRELLAKLP KPKND YELM EPRFADETDV EATVGVLEED ATDRERRIQE RIAEKERLAK ARRSQVIQRD LIRPSVTQPE KWKRSLENED PTANVL LKE MIALISSDAI NYPFGNSKVK GTANKVPDLS NEEIERCRLL LKKEIGQLES DDYIQFEKEF LETYSALHNT SSLLPGL VI YEEDDEDVEA AEKFYTNDIQ RDLAKKALEC NKLENRVYDL VRSSYEQRNF LIKKISHAWK ALQTERKNLT CYEFLYNQ E RLALPNRLEA AEIELSKMQQ IEAYAQQDYA RVTGQN

UniProtKB: Pre-mRNA-splicing factor cdc5

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Macromolecule #25: Pre-mRNA-splicing factor cwf3

MacromoleculeName: Pre-mRNA-splicing factor cwf3 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 92.759914 KDa
SequenceString: MGDVIPLKVN FDLINVDDEP FELELLRDPY SLKSWLRYIK THEGSTLEKR VLLFERACSE LPGSYKIWKS YLELRVAHVE HLNPYFHAE AFASVNDCFE RSLILLHKMP VIWKLYLQFL MKQPNVTKIR CTFNSALRAL PVTQHDDIWD MFTKYAEDIG G LFCIHVYR ...String:
MGDVIPLKVN FDLINVDDEP FELELLRDPY SLKSWLRYIK THEGSTLEKR VLLFERACSE LPGSYKIWKS YLELRVAHVE HLNPYFHAE AFASVNDCFE RSLILLHKMP VIWKLYLQFL MKQPNVTKIR CTFNSALRAL PVTQHDDIWD MFTKYAEDIG G LFCIHVYR RYIQVEPRAI ENYIEILCKL GLWNEAARQY EDILNRPVFL SAKRKSNYQI WLEFSELVVQ HPDHTQNIDV EK VFRAGIK RFSDQAGKLW TYLAQYYIRI GDYEKARSTF YEGMNNIMTV RNFTIIFDAF VEFEEQWLSA RVEASSGNAN DEL SIDFHM AWLEKILDKR PLYINDVLLR QNINNVDEWL RRVKFLEDDS EKVVQVYTDA IKNVNPKLAH GSLGKLFSEF ARFY ENFDD LEQSRIIFEK ATHVPYKTVN ELAQVWIDWA EMELRHQNFD AARKLIGDAV HAPRKSHISF FDESLSPQVR LHKSS KIWM YYLDLEESVG TIETTRKLYD RVFELKIATP QVVVNYANLL EENAYFEDSF KIYERGVALF SYPVAFELWN LYLTKF VKR YQGTHMERTR DLFEQALEGC PPEFSKSIYL LYADFEEKFG KAKRSISILE KAADKVKTAD RLAIYNVLLV KVALNYG VL ATRTVYEKAI ESLSDSEVKD MCLRFAEMET KLGEIDRARL IYIHGSQYCD PRVETDYWKA WQEFEIRYGN PEETVKEM L RIKRSVQTKF STDSLHIAKR AAKIESAAAP MDPMEQLEME KSEGPKALAG FVLSKSNPQE TSKITGEEN

UniProtKB: Pre-mRNA-splicing factor cwf3

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Macromolecule #26: Pre-mRNA-splicing factor syf2

MacromoleculeName: Pre-mRNA-splicing factor syf2 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 28.037457 KDa
SequenceString: MDSPSHSREA RVEKLKKIRE QMRKSSHENR KEVVQEHSRM RIDPALERRL ERKRMEAEEE LAKIETEEEG GDFERRRAWD WTIEESEKW DQRLRKKKQT IQNVAFSDYH SQASKDYNRG IRDLKPDYEK YDKDKKEEKS KSRSVALYDE AERLDWVSNK P DKEHVERL ...String:
MDSPSHSREA RVEKLKKIRE QMRKSSHENR KEVVQEHSRM RIDPALERRL ERKRMEAEEE LAKIETEEEG GDFERRRAWD WTIEESEKW DQRLRKKKQT IQNVAFSDYH SQASKDYNRG IRDLKPDYEK YDKDKKEEKS KSRSVALYDE AERLDWVSNK P DKEHVERL VESIKKQDQR RLKNTKRRGT DEEDHITFIN ERNRKFNLKL QRFYSKYTKD IKEDLERGTA L

UniProtKB: Pre-mRNA-splicing factor syf2

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Macromolecule #27: Pre-mRNA-splicing factor cwf7

MacromoleculeName: Pre-mRNA-splicing factor cwf7 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 21.348246 KDa
SequenceString:
MSYNISLDSL PYFESTYNEE DRSAAAQIVE EELKRSGGLL IKQEEQKKKF QSHRLSDRLE NAIVAAGKGE KLAAIDVQRY IQLKPPGTR ESLLQSAVVW EYQKSRNDNL YLLEKHGEKA FDSSLEALEV QLELLEKELV NTKKLSQGCN RKRKHYQMEV G ARLAEAEV KFGQLLQSSI QCRVATLLS

UniProtKB: Pre-mRNA-splicing factor cwf7

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Macromolecule #28: Pre-mRNA-processing factor 17

MacromoleculeName: Pre-mRNA-processing factor 17 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 63.211309 KDa
SequenceString: MLVANYSSDS EEQENSQSPN IQPLLHTENL APAVVDNVKD DLIVSKGGNS RELARNVPVN EMVQPALGPA NPFVTKEQDS IKNSITGYA EREYVPNFVF NQEYYANTHA IYGKRNFDDN EATTSTDLKR KSQKIKERRE DPGDPSILEG DGAYKGPWAG Y GSEQSSSP ...String:
MLVANYSSDS EEQENSQSPN IQPLLHTENL APAVVDNVKD DLIVSKGGNS RELARNVPVN EMVQPALGPA NPFVTKEQDS IKNSITGYA EREYVPNFVF NQEYYANTHA IYGKRNFDDN EATTSTDLKR KSQKIKERRE DPGDPSILEG DGAYKGPWAG Y GSEQSSSP LEYSEYEEVE SLDVKSEKDT ESNNLGQNEL LKEQLATPEV ETHRSKEETI LHKDRLFDYQ NRSYMHVPND VG INLSEEP GEQTCYIPKK HIFTWKGHTK GISCLRFFPI SGHLLLSGSM DNQIKIWEVY HDRSLLRTFQ GHARPIRDLS FSQ DGRSFL STSFDKTIKL WDTELGKCLN CFNSDRLTNC VKFQVDPDKP NEFLAGTADK RILQFDIRSP DIVQAYDHHL GGIN SITFL ENGKRFVTTS DDSSMRFWEY GTPVPIKFVA DIAMHSMPRV ALRPNGKSIA CQSLDNCIYV YSAYEKYRQN KKKVF KGYS CSGYSLEVGF SPDGRFVFSG DSSGNACFWD WKTCKLMAKL PAHSGPVQSM AFHPQETSKV ATSSIVDGSI KYWD

UniProtKB: Pre-mRNA-processing factor 17

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Macromolecule #29: Pre-mRNA-splicing factor cwf21

MacromoleculeName: Pre-mRNA-splicing factor cwf21 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 34.617762 KDa
SequenceString: MSYNGIGLPT PRGSGTNGYV MRNLSHVKKY DKNTNLQSNR NAKALEKRVQ DPSISEHECR RQIESKLLLY REQLLEEVSS QHSTDAAAS DSNTNFGTEN PKPPKAIIKD EQSQSKTKSL DEADVEILVQ KYREQLLKEL QLQKSTEKGK NFESILQPQK R KETRGFHS ...String:
MSYNGIGLPT PRGSGTNGYV MRNLSHVKKY DKNTNLQSNR NAKALEKRVQ DPSISEHECR RQIESKLLLY REQLLEEVSS QHSTDAAAS DSNTNFGTEN PKPPKAIIKD EQSQSKTKSL DEADVEILVQ KYREQLLKEL QLQKSTEKGK NFESILQPQK R KETRGFHS KNDDDGRLEE RDDLRSSVDD DYYDYPRYSE RKSLNSKRHV DNYNENRRRH YDSYSSYDEL ERRRSSNESY SR RSELPRR DYNRHDERER YSYHRRRERS NSPSYTKNES IPVVDRDSSP EGGEIV

UniProtKB: Pre-mRNA-splicing factor cwf21

+
Macromolecule #30: Pre-mRNA-splicing factor cwf22

MacromoleculeName: Pre-mRNA-splicing factor cwf22 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 102.928359 KDa
SequenceString: MEKEDKSFGI GMLDYNRENP ESSGHSRVAV IRKQKTEQEN NNLSWEDRHY IPDLHKSNKI KTPTSLADEK KSHNELDPKA QIKKLMETR SGGTYIPPAK LKALQAQLTD VNTPEYQRMQ WEALKKSING LINKVNKSNI RDIIPELFQE NIIRGRALYC R SIMKAQAA ...String:
MEKEDKSFGI GMLDYNRENP ESSGHSRVAV IRKQKTEQEN NNLSWEDRHY IPDLHKSNKI KTPTSLADEK KSHNELDPKA QIKKLMETR SGGTYIPPAK LKALQAQLTD VNTPEYQRMQ WEALKKSING LINKVNKSNI RDIIPELFQE NIIRGRALYC R SIMKAQAA SLPFTPIYAA MTAVINTKFP QIGELLLTRL IVQFRKSFQR NDKSMCISSS SFIAHLINQK IAHEIVGLQI LA VLLERPT NDSIEIAVML LREIGAYLAE VSTRAYNGVF ERFRTILHEG QLERRTQFII EVLFQTRKDK FKNNPTIPQE LDL VEEEDQ ITHYISLDDN LDVQESLGIF HYDPDYEENE KKYDAIKHEI LGEEDDDENE EDEEDSEETS ESEEDESVND EKPQ VIDQT NASLVNLRKS IYLTIMSSVD FEECCHKLLK IDLPEGQEIE LCNMVIECNS QERTYAKFYG LIGERFCKLS RTWRS TYEQ CFKNYYETIH RYETNRLRNI ALFFANLLST DSIGWEVYDC VRLTEDDTTA SSRIFLKIMF QEIVEALGLK SLVERL HDP NLVPYLHGLF PVDEARNVRF SINYFTSIGL GALTEEMREY LLTMPKSEPK EQDSEGYSSG SETGSTYSSS YSSTYSR GR SYSRSTRSYS KSRSYSRSRS VTPINNINHK KYIRKDRELS PRGRERSSNR NSYSDLSRSS SLSRGRSRSY TPEGRLIE S EDKGYRSRSS SPASRKYRSR QRYRRSYAGS TSRGRSFSRS PSYRRRLSMS CSVSYSRSPS PAARPISRSP ARNKRSYDS LSYNRQYSPK VSRKRSNESR SPSPYTLRKQ KTYHLNKRNE DFVVKMDKKG SESPVILAPW LREVDDGELY KDRNSESDSV RKQPRAD

UniProtKB: Pre-mRNA-splicing factor cwf22

+
Macromolecule #31: Peptidyl-prolyl cis-trans isomerase ppi1

MacromoleculeName: Peptidyl-prolyl cis-trans isomerase ppi1 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 16.884201 KDa
SequenceString:
MANVELQTSL GKILIELYTE HAPKTCQNFY TLAKEGYYDG VIFHRVIPDF VIQGGDPTGT GRGGTSIYGD KFDDEIHSDL HHTGAGILS MANAGPNTNS SQFFITLAPT PWLDGKHTIF GRVVSGLSVC KRMGLIRTDS SDRPIEPLKI IKAVAL

UniProtKB: Peptidyl-prolyl cis-trans isomerase ppi1

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Macromolecule #32: UNK1

MacromoleculeName: UNK1 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 1.890321 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

+
Macromolecule #33: G-patch domain-containing protein C1486.03

MacromoleculeName: G-patch domain-containing protein C1486.03 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 91.79118 KDa
SequenceString: MQNEYVDMNS SSEDSDGDSI LEEGRLRPSF RGQQKERDML GIFGEEDEDG FHNSGIGSAR LRRKNISFVE KSEQVKANKQ VTADDLLEA HSIPQLKNKN DEVSQAKNIP KMKFNTTGFG AKMLEKMGYK QGQGLGANAE GIAEPVQSKL RPERVGLGAV R ERTEKQRK ...String:
MQNEYVDMNS SSEDSDGDSI LEEGRLRPSF RGQQKERDML GIFGEEDEDG FHNSGIGSAR LRRKNISFVE KSEQVKANKQ VTADDLLEA HSIPQLKNKN DEVSQAKNIP KMKFNTTGFG AKMLEKMGYK QGQGLGANAE GIAEPVQSKL RPERVGLGAV R ERTEKQRK EAIARGEISD SEDEKHTVKQ KPLREKKKKP LKSSEEISKD MGSYNLPRFL ASLIDASLND TKEIEFVTSN KE ELGLEGR DMSTSGINQL SRLARVECEH HASAWQQLQA RRAYVKMELK RVTTEFDEKS VEISRLEKLL GKVMEVKSRS MEF TVPEAE IDVIEKRLQP LNNLIETLPV EFSEASMHFE LDSVAVSILA FVLSEPIKNW DVWKHPYFML ESFLSWKNSL YSKD FRPKR EESSTFMDID VEFDDELEGQ SLTHYESFMM FVWKKKIGEE LKKWIIQDSL KALQLLEAWD PVVPEKVKDS LIQDD ILPR LKDAVSKWNP KLKLKKNDSL HHCIFPWLPY LEKHADSLLQ SVLVQFSLIL SPWKIKNGSI DDFSVWRSAF ANDALD RLL EKVILPKLEK LMDEELVIDP SNQDLEIFFI ILSWKGSFKA MVFGQLFADH FFPKWLETLY QWLTEAPNFD EASEWYT WW KSVFPKDLLS NAYIQQGFSK GLDMMNECLE NKSITAPLPF AKDSTKGVNL QFSKEKHEFT AESDDTTSYD EPLVSFRE L VEEFCAENSL LFVPLRRSHL STGSALFRIS TQASKARGIT VYLRNDIIWK KSPGASEDTP YDPIGFNEIL LMFNNN

UniProtKB: G-patch domain-containing protein C1486.03

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Macromolecule #34: UNK2

MacromoleculeName: UNK2 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 29.464186 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

+
Macromolecule #35: Uncharacterized protein C20H4.06c

MacromoleculeName: Uncharacterized protein C20H4.06c / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 60.85007 KDa
SequenceString: MYANSRKRSN VDVHRHPYVV YGSPFDLEPS RINQGVPVWK QEARDERNRK RFHGAFTGGF SAGYFNTVGS KEGWQPKSWK SSRNENKSV HGMTIDDIMD EEDRADQELS KVYTSKINTI DTSIPIDDPL LREFSNRDDS IGEKMLKNLG WNGHDFVISP F QGYYDDTK ...String:
MYANSRKRSN VDVHRHPYVV YGSPFDLEPS RINQGVPVWK QEARDERNRK RFHGAFTGGF SAGYFNTVGS KEGWQPKSWK SSRNENKSV HGMTIDDIMD EEDRADQELS KVYTSKINTI DTSIPIDDPL LREFSNRDDS IGEKMLKNLG WNGHDFVISP F QGYYDDTK LTNQAYHGIG YQADDVFQLE RKPVGMAKNL RGGFGSGVLN DDGIEDQDIY DLGAPKMRYD KSINITKKVA SP FTPVKHT FLSKKDRSKG KSIIKSQNCS DGLPPLPGFV VVFNLQSPFQ NNWFPPPTIP EGWEPKLFTN SQGFSQKKAQ TSN ERLPLF AEKSETNSNG EASLPSDVNH GVKEDEGSWQ LIDIETAKNA MSRKDNPYND ERAGIYEIFL KAHLMNMPRL LRDI DSRHL LEFTQTASLY RPMSKNLSMR FVSSFEASTK KESINSHSET DDSILRRPRT VVTFIPERLL CKRFNVALPY GQEKQ KRKN ISSIENVPII AEIENKSIDS IDTRKLNRPQ DIEEKPKAPQ TLFSAIFGDD

UniProtKB: Uncharacterized protein C20H4.06c

+
Macromolecule #36: Putative pre-mRNA-splicing factor ATP-dependent RNA helicase C20H4.09

MacromoleculeName: Putative pre-mRNA-splicing factor ATP-dependent RNA helicase C20H4.09
type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 73.113695 KDa
SequenceString: MPNEFISQSL STLTNTPLNI QKKLLPITKY RNQLLYAVEQ NQITIVLGHT GCGKTTQIPQ FLYEAGWASQ NGIIGCTQPR RLVAKSVSE RVSLELNSPP GSLCGYSIQF DHNVSEKTKI KYMTDGILLN EIFFDPLLER YSIVILDEVH ERTLSTDLLL G VLKRILEK ...String:
MPNEFISQSL STLTNTPLNI QKKLLPITKY RNQLLYAVEQ NQITIVLGHT GCGKTTQIPQ FLYEAGWASQ NGIIGCTQPR RLVAKSVSE RVSLELNSPP GSLCGYSIQF DHNVSEKTKI KYMTDGILLN EIFFDPLLER YSIVILDEVH ERTLSTDLLL G VLKRILEK RNDFRLVLSS ASVDANKLSQ FFGQDKVCTM SIEGKLFPVE TLFLQKPTEN YVDSAIETVI NINSTYPPGD IL VFLSGRK EIEYCIKKIE DSLIHASEDC QTLVPLPLHA GLTVDEQMRV FNIYDGDFRK VIFSTNIAET SITIDGIVYV VDS GFNKQR IYNPYTRTSK LINVPISKSS AIQRSGRAGR TMRGKVFRLY TEKAYSLMKE EFEADILNCD MSPLVLFLKG LGLK NILQF PFFVRPPTVH LMAALEDLYL LGVLDESGNL TDPLGIQISN SFLDANISKA LLTSNQFGCT HEILSIASIL TAGEV FYNP TSSSKNDAFV AHSSFFANEG DIITALNVFE SFVGNKKDLQ WCRKNYLNYQ TLRQALDIRT HLVRFLNKFS IPTAQR LPS SDCSKILKCL LDGFVRNVAH LQNDGSYKTI GGKQVWLDSS SVLHEKKTPW IMYSSAVESE TQIFVKNISK IESFWLD KY YKREK

UniProtKB: Putative pre-mRNA-splicing factor ATP-dependent RNA helicase C20H4.09

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Macromolecule #37: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 37 / Number of copies: 4 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #38: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 38 / Number of copies: 1 / Formula: K
Molecular weightTheoretical: 39.098 Da

+
Macromolecule #39: INOSITOL HEXAKISPHOSPHATE

MacromoleculeName: INOSITOL HEXAKISPHOSPHATE / type: ligand / ID: 39 / Number of copies: 1 / Formula: IHP
Molecular weightTheoretical: 660.035 Da
Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE

+
Macromolecule #40: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 40 / Number of copies: 1 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

+
Macromolecule #41: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 41 / Number of copies: 6 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.5
Component:
ConcentrationFormulaName
20.0 mMC8H18N2O4SHEPES
100.0 mMNaClSodium chloride
1.5 mMMgCl2Magnesium chloride
0.01 %(C2H4O)nC14H22OIGEPAL CA-630

Details: 20 mM HEPES pH 7.5, 100 mM NaCl, 1.5 mM MgCl2, 0.01% IGEPAL CA-630
GridModel: Quantifoil R2/1 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
SoftwareName: SerialEM
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 1 / Number real images: 13096 / Average exposure time: 1.491 sec. / Average electron dose: 49.4 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.6 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Particle selectionNumber selected: 929930
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 61423
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

-
Atomic model buiding 1

RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-9esh:
Structure of a B-state intermediate committed to discard (Bd-I state)

+
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