EMDB-19877: Cryo-EM structure of the Plastid-encoded RNA polymerase from Sina...

Basic information

Entry

Database: EMDB / ID: EMD-19877

• Title: Cryo-EM structure of the Plastid-encoded RNA polymerase from Sinapis alba

Sample

• Complex: Plastid-encoded RNA polymerase
  • Protein or peptide: x 19 types
• Ligand: x 6 types

• Keywords: RNA polymerase Chloroplast Sinapis alba Cryo-EM / TRANSCRIPTION

Function / homology

Function:

: / DNA-directed RNA polymerase complex / chloroplast / : / : / : / : / : / : / DNA-directed RNA polymerase / protein dimerization activity / magnesium ion binding / mitochondrion / DNA binding / zinc ion binding

Domain/homology:

: / DNA-directed RNA polymerase subunit RpoC1 / DNA-directed RNA polymerase, subunit beta'' / RNA polymerase, alpha subunit, C-terminal / Bacterial RNA polymerase, alpha chain C terminal domain / DNA-directed RNA polymerase, alpha subunit / DNA-directed RNA polymerase, subunit beta-prime / RNA polymerase Rpb1, domain 3 superfamily / DNA-directed RNA polymerase, insert domain / DNA-directed RNA polymerase, RpoA/D/Rpb3-type / RNA polymerase Rpb3/RpoA insert domain / RNA polymerase Rpb3/Rpb11 dimerisation domain / RNA polymerases D / RNA polymerase Rpb1, clamp domain superfamily / RNA polymerase Rpb1, domain 1 / RNA polymerase Rpb1, domain 1 / RNA polymerase, alpha subunit / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 2 / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / DNA-directed RNA polymerase, insert domain superfamily / RNA polymerase, N-terminal / RNA polymerase Rpb1, funnel domain superfamily / RNA polymerase I subunit A N-terminus / RNA polymerase, RBP11-like subunit

Component:

DNA-directed RNA polymerase subunit beta' / DNA-directed RNA polymerase subunit alpha / DNA-directed RNA polymerase subunit beta''

• Biological species: Sinapis alba (white mustard)
• Method: single particle reconstruction / cryo EM / Resolution: 3.0 Å
• Authors: Effantin G / Blanvillain R / Cobessi D

Funding support

France, 1 items

Organization	Grant number	Country
Agence Nationale de la Recherche (ANR)	ANR-17-CE11-0031	France

• Citation: Journal: To Be Published; Title: Cryo-EM structure of the Plastid-encoded RNA polymerase from Sinapis alba; Authors: Effantin G / Blanvillain R / Cobessi D

History

Deposition	Mar 18, 2024	-
Header (metadata) release	Jun 18, 2025	-
Map release	Jun 18, 2025	-
Update	Jun 18, 2025	-
Current status	Jun 18, 2025	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_19877.map.gz / Format: CCP4 / Size: 163.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.06 Å

Density

Contour Level	By AUTHOR: 0.00912
Minimum - Maximum	-0.0009843957 - 0.04951311
Average (Standard dev.)	0.00042692662 (±0.0018236921)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 350 350 350 Spacing 350 350 350
Cell	A=B=C: 370.99997 Å α=β=γ: 90.0 °

Supplemental data

Sample components

Entire : Plastid-encoded RNA polymerase

• Entire: Name: Plastid-encoded RNA polymerase

Components

• Complex: Plastid-encoded RNA polymerase
  • Protein or peptide: DNA-directed RNA polymerase subunit alpha
  • Protein or peptide: RpoB, subunit beta
  • Protein or peptide: DNA-directed RNA polymerase subunit beta'
  • Protein or peptide: DNA-directed RNA polymerase subunit beta''
  • Protein or peptide: PAP1, pTAC3
  • Protein or peptide: PAP2, pTAC2
  • Protein or peptide: PAP3, pTAC10
  • Protein or peptide: PAP4, FSD3
  • Protein or peptide: PAP5, pTAC12, HEMERA
  • Protein or peptide: PAP6, FLN1
  • Protein or peptide: PAP7, pTAC14
  • Protein or peptide: PAP8, pTAC6
  • Protein or peptide: PAP9, FSD2
  • Protein or peptide: PAP10, TRXz
  • Protein or peptide: PAP11, MurE-like
  • Protein or peptide: PAP12, pTAC7
  • Protein or peptide: FLN2
  • Protein or peptide: pTAC18
  • Protein or peptide: PRIN2, PLASTID REDOX INSENSITIVE 2
• Ligand: 2,3-DIHYDROXY-1,4-DITHIOBUTANE
• Ligand: ZINC ION
• Ligand: FE (III) ION
• Ligand: CALCIUM ION
• Ligand: POTASSIUM ION
• Ligand: S-ADENOSYL-L-HOMOCYSTEINE

Supramolecule #1: Plastid-encoded RNA polymerase

• Supramolecule: Name: Plastid-encoded RNA polymerase / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#19
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 1.15 MDa

Macromolecule #1: DNA-directed RNA polymerase subunit alpha

• Macromolecule: Name: DNA-directed RNA polymerase subunit alpha / type: protein_or_peptide / ID: 1 / Details: S67F mutation found from electron density analyses / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 37.94575 KDa

Sequence

String:

MVREKVKVST RTLQWKCVES RRDSKRLYYG RFILSPLMKG QADTIGIAMR RALLGEIEGT CITRAKFENI PHDYSNIVGI QESVHEILM NLNEIVLKSN LYGTRNALIC VQGPGYITAR DIILPPSVEI VDNTQHIATL TEPINLCIGL KIERNRGYSL K MSNNFEDR SYPIDAVFMP VQNANHSIHS YGNGNEKQEI LFIEIWTNGS LTPKEALHEA SRNLINLFIP FLHVEEETFY LE NNQHQVT LPLFPFHNKL VNLRQKKKEL AFQYIFIDQL ELPPRIYNCL KKSNIHTLLD LLNNSQEDLI KMEHFHIEDV KKL LDILEK K

UniProtKB: DNA-directed RNA polymerase subunit alpha

Macromolecule #2: RpoB, subunit beta

• Macromolecule: Name: RpoB, subunit beta / type: protein_or_peptide / ID: 2 / Details: S113F muation found from electron density analysis / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 121.223156 KDa

Sequence

String:

MLGDGKEGTS TIPGFNQIQF EGFYRFIDQG LIEELSKFPK IEDIDHEIEF QLFVETYQLV EPLIKERDAV YESLTYSSEL YVSAGLIWK TNRNMQEQRI FIGNIPLMNS LGTFIVNGIY RVVINQILQS PGIYYQSELD HNGISVYTGT IISDWGGRLE L EIDKKARI WARVSRKQKI SILVLSSAMG SNLREILENV CYPEIFLSFL TDKEKKKIGS KENAILEFYQ QFSCVGGDPI FS ESLCKEL QKKFFHQRCE LGRIGRRNIN WRLNLNIPQN NIFLLPRDIL AAADHLIGMK FGMGTLDDMN HLKNKRIRSV ADL LQDQLG LALARLENVV KGTIGGAIRH KLIPTPQNLV TSTPLTTTYE SFFGLHPLSQ VLDRTNPLTQ IVHGRKLSYL GPGG LTGRT ANFRIRDIHP SHYGRICPID TSEGINVGLI GSLSIHARIG DWGSLESPFY ELVEKSKKAQ IRMLFLSPSQ DEYYM IAAG NSLALNRGIQ EEQVVPARYR QEFLTIAWEE VHLRSIFPFQ YFSIGASLIP FIEHNDANRA LMSSNMQRQA VPLSRS EKC IVGTGLERQV ALDSGVPAIA EHEGKILYTD TEKIILSGNE NTLSIPLIMY QRSNKNTCMH QKPQVRRGKC IKKGQIL AD GAATVGGELA LGKNILVAYM PWEGYNFEDA VLISECLVYG DIYTSFHIRK YEIQTHVTTQ GPERITKEIP HLEGRLLR N LDKNGIVMLG SWVETGDILV GKLTPQVAKE SSYAPEDRLL RAILGIQVST SKETCLKLPI GGRGRVIDVR WVQKKGGSS YNPEIIRVYI SQKREIKVGD KVAGRHGNKG IISKILPRQD MPYLQDGRPV DMVFNPLGVP SRMNVGQIFE CSLGLAGSLL DRHYRIAPF DERYEQEASR KLVFSELYEA SKQTANPWVF EPEYPGKSRI FDGRTGDPFE QPVIIGKPYI LKLIHQVDDK I HGRSSGHY ALVTQQPLRG RSKQGGQRVG EMEVWALEGF GVAHILQEML TYKSDHIRAR QEVLGTTIIG GTIPKPEDAP ES FRLLVRE LRSLALELNH FLVSEKNFQI NRKEV

Macromolecule #3: DNA-directed RNA polymerase subunit beta'

• Macromolecule: Name: DNA-directed RNA polymerase subunit beta' / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 78.761867 KDa

Sequence

String:

MIDRYKHQQL RIGLVSPQQI SAWATKKIPN GEIVGEVTKP YTFHYKTNKP EKDGLFCERI FGPIKSGICA CGNYRVIGDE KEDPKFCEQ CGVEFVDSRI RRYQMGYIKL TCPVTHVWYL KRLPSYIANL LDKPLKELEG LVYCDFSFAR PITKKPTFLR L RGSFEYEI QSWKYSIPLF FTTQGFEIFR NREISTGAGA IREQLADLDL RIIIENSLVE WKQLGEEGPT GNEWEDRKIV RR KDFLVRR MELAKHFIRT NIEPEWMVLC LLPVLPPELR PIIQIEGGKL MSSDINELYR RVIYRNNTLT DLLTTSRSTP GEL VMCQEK LVQEAVDTLL DNGIRGQPMR DGHNKVYKSF SDVIEGKEGR FRETLLGKRV DYSGRSVIVV GPSLSLHRCG LPRE IAIEL FQTFVIRGLI RQHLASNIGV AKSQIREKKP IVWEILQEVM QGHPVLLNRA PTLHRLGIQS FQPILVEGRT ICLHP LVCK GFNADFDGDQ MAVHVPLSLE AQAEARLLMF SHMNLLSPAI GDPISVPTQD MLIGLYVLTS GTRRGICANR YNPCNR KNY QNERIYETNY KYMKEPFFCN SYDAIGAYRQ KRINLDSPLW LRWQLDQRVI ASKEVPIEVH YESFGNYHEI YAHYLIV RS VKKETLYIYI RTTVGHISFY REIEEAIQGF SQACSYDT

UniProtKB: DNA-directed RNA polymerase subunit beta'

Macromolecule #4: DNA-directed RNA polymerase subunit beta''

• Macromolecule: Name: DNA-directed RNA polymerase subunit beta'' / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 156.388719 KDa

Sequence

String:

MAERANLVFH NKVIDGTAIK RLISRLIDHF GMAYTSHILD QVKTLGFQQA TATSISLGID DLLTIPSKGW LVQDAEQQSL ILEKHHHYG NVHAVEKLRQ SIEIWYATSE YLRQEMNPNF RMTDPFNPVH MMSFSGARGN ASQVHQLVGM RGLMSDPQGQ M IDLPIQSN LREGLSLTEY IISCYGARKG VVDTAVRTSD AGYLTRRLVE VVQHIVVRRT DCGTIRGISV SPRNKSRMMS ER IFIQTLI GRVLADDIYI GSRCVAFRNQ DLGIGLVNRF ITFGTQSISI RTPFTCRSTS WICRLCYGRS PTHGDLVELG EAV GIIAGQ SIGEPGTQLT LRTFHTGGVF TGGTAEHVRA PYNGKIKFNE DLVHPTRTRH GHPAFLCYID LSVIIESEDI IHSV TIPPK SFLLVQNDQY VESEQVIAEI REGTYTFHFK ERVRKYIYSD SEGEMHWSTD VSHAPEFTYS NVHLLPKTSH LWILS GGSC GSSLILFSIH KDQDQMNIPF LSVERKSISS LSVNNDQVSQ KFFSSDFSDK KKSGIPNYSE LNGIVGTSHY NFIYSA IFH ENSDLLAKRR RNRFLIPFQS IQEQEQEKEF IPHSGISVEI PINGIFRRNS IFAFFDDPRY RRKSSGILKY GTLKADS II QKEDMIEYRG VQKFKTKYEM KVDRFFFIPE EVHILPESSA IMVENYSIIG VDTRITLNIR SQVGGLIRVE RKKKRIEL K IFSGDIHFPD KTDKISRHSG ILIPPGRGKT NSKESKNLKN WIYVQRITPT KKKFFVLVRP VATYEIADSI NLATLFPKD LFREKDNIQL RVFNYILYGN GKPTRGISDT SIQLVRTCLV LNWDQDNKNS SLEEVRAFFV EVNTKGLIRD FIRIGLVKSH ISYIRKRNN PPDSGLISAD SMNPFYSISP KAGILHQSLR QNHGTIRMFL NRNKESQSLL ILSSSNCFRI GPFNHVKYHN V INQSIKKK PLITIKNSSG PLGTAIQISN FYSFLPLLTY NQISVIKYLQ LDNFKYIFQV IHSYLIDENG RIFNLDPYSN LV LNPFKLN WYFLHQNYNN NYCEETSTII SLGQFFCENV CIAKKEPYLK SGQVLIVQRD SVVIRSAKPY LATPGAKVHG HYR EILYEG DTLVTFIYEK SRSGDITQGL PKVEQVLEVR SIDSISLNLE KRIKGWNRCI TRILGIPWGF LIGAELTIVQ SRIS LVNKI QKVYRSQGVQ IHNRHIEIIV RQITSKVLVS EEGMSNVFLP GELIGLLRAE RTGRALEEAI CYRAVLLGIT RASLN TQSF ISEASFQETA RVLAKAALRG RIDWLKGLKE NVVLGGVIPA GTGFNKGLVH CSRQHTNILL EKKTKNLSLL EGDMRD ILF YHREFCDSSI

UniProtKB: DNA-directed RNA polymerase subunit beta''

Macromolecule #5: PAP1, pTAC3

• Macromolecule: Name: PAP1, pTAC3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 103.467867 KDa

Sequence

String:

MSLFFLNPAL PSNSIHPIPR RAAGISSIRC SISAPEKKPR RRRKQQQKRE NEDSSSFGSS EAVSALERSL RLTFMDELME RARNRDPSG VSEVIYDMIA AGLSPGPRSF HGLVVAHALN GDEQGAMHSL RKELGAGQRP LPETMIALVR LSGSKGNAQR G LELLAAME KLNYDIRQAW LILVEELVRT NHLEEANKVF LKGARGGMRA TDQLYDLMIE EDCKAGDHSN ALDISYEMEA AG RFATTFH FNCLLSVQAT CGIPEVAYAT FENMEYGEDF MKPDTETYNW VIQAYTRADS YDRVQDVAEL LGMMVEDYKR VQP NVKTHA LLVECFTKYC VVKEAIRHFR ALKNFEGGTK VLHNAGNFED PLSLYLRALC REGRIVELID ALDAMRRDNQ PIPP RAMIM SRKYRTLVSS WIEPLQEEAE LGYEIDYLAR YVEEGGLTGE RKRWVPRRGK TPLDPDAAGF IYSNPIETSF KQRCL EDWK VHHRKLLRTL QSEGLPVLGD ASESDYMRVM ERLRNIIKGP AQNLLKPKAA SKMVVSELKE ELEAQGLPID GTRNVL YQR VQKARRINKS RGRPLWVPPI EEEEEEVDEE VDELICRIKL HEGDTEFWKR RFLGEGLIET TAETKETDES SVATGEI EN KTEVVAKEAD DDEDDEEEEQ EGDEDDDENE EEEEAVVVEP ENRAEGEDLI KNKAADAKRH LQMIGVQLLK ESDEANRT K KRGKRASRMT LEDDADEDWF PEEPFEAFKE MRERKVFDVS DMYTIADVWG WTWEKDFKNK TPRRWSQEWE VELAIVLMA KVIELGGVPT IGDCAVILRA AIRAPMPSSF LKILQTTHSL GYAFGSPLYD EIITLCLDLG ELDAAIAIVA DMETTGITVP DQTLDKVIS ARQSNEIPKS EHEEPPSSSE SS

Macromolecule #6: PAP2, pTAC2

• Macromolecule: Name: PAP2, pTAC2 / type: protein_or_peptide / ID: 6 ; Details: Full sequence: MNLAIPNPNSHHLSFLIQNSTFIGNRRFANSNHLSFLSGGKRPCSVAKINAKTKDLVLGNPSVSVEKGKYSYDVESLINK LSSLPPRGSIARCLDIFKNKLSLNDFALVFKEFAGRGDWQRSLRLFKYMQRQIWCKPNEHIYTIMISLLGREGLLDKCLE VFEEMPSQGVARSVFSYTALINAYGRNGRYETSLELLDRMKSEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEG IQPDIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLGRLEKVSDLLSEMASGGSLPDITSYNV LLEAYAKSGSIKEAMGVFHQMQAAGCSPNANTYSVLLNLFGQNGRYDDVRQLFLEMKSSNTDPDAATYNILIDVFGEGGY FKEVVTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTAKDVVPSSKAYTGVIEAFGQAALYEEALVAFNT MHEVGSNPSIETFHSLLYSFARGGLFKESEVILSRLVNSGIPRNRDTFNATIEAYKQGGKFEEAVKTYVDMEKSRCDPDE RTLEAVLSVYSCARLVDECREQFEEMKASDILPSIMCYCMMLSVYGKTESWDDVNELLEEMLSNRVSNIHQVIGQMIKGN YDDDSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGIFPELFRKNKLVWSVDVHRMSEGG MYTALSVWLNDLSDILVKGQDLPQLAVVVSVRGQLEKSSAARESPITRAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLK QLLSTQEPTSEESPKSNIVALTNSPIFAAGTRTSTSSDTNHSGGNPSQRKTKMKKELAGSPA,Full sequence: MNLAIPNPNSHHLSFLIQNSTFIGNRRFANSNHLSFLSGGKRPCSVAKINAKTKDLVLGNPSVSVEKGKYSYDVESLINK LSSLPPRGSIARCLDIFKNKLSLNDFALVFKEFAGRGDWQRSLRLFKYMQRQIWCKPNEHIYTIMISLLGREGLLDKCLE VFEEMPSQGVARSVFSYTALINAYGRNGRYETSLELLDRMKSEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEG IQPDIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLGRLEKVSDLLSEMASGGSLPDITSYNV LLEAYAKSGSIKEAMGVFHQMQAAGCSPNANTYSVLLNLFGQNGRYDDVRQLFLEMKSSNTDPDAATYNILIDVFGEGGY FKEVVTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTAKDVVPSSKAYTGVIEAFGQAALYEEALVAFNT MHEVGSNPSIETFHSLLYSFARGGLFKESEVILSRLVNSGIPRNRDTFNATIEAYKQGGKFEEAVKTYVDMEKSRCDPDE RTLEAVLSVYSCARLVDECREQFEEMKASDILPSIMCYCMMLSVYGKTESWDDVNELLEEMLSNRVSNIHQVIGQMIKGN YDDDSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGIFPELFRKNKLVWSVDVHRMSEGG MYTALSVWLNDLSDILVKGQDLPQLAVVVSVRGQLEKSSAARESPITRAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLK QLLSTQEPTSEESPKSNIVALTNSPIFAAGTRTSTSSDTNHSGGNPSQRKTKMKKELAGSPA,Full sequence: MNLAIPNPNSHHLSFLIQNSTFIGNRRFANSNHLSFLSGGKRPCSVAKINAKTKDLVLGNPSVSVEKGKYSYDVESLINK LSSLPPRGSIARCLDIFKNKLSLNDFALVFKEFAGRGDWQRSLRLFKYMQRQIWCKPNEHIYTIMISLLGREGLLDKCLE VFEEMPSQGVARSVFSYTALINAYGRNGRYETSLELLDRMKSEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEG IQPDIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLGRLEKVSDLLSEMASGGSLPDITSYNV LLEAYAKSGSIKEAMGVFHQMQAAGCSPNANTYSVLLNLFGQNGRYDDVRQLFLEMKSSNTDPDAATYNILIDVFGEGGY FKEVVTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTAKDVVPSSKAYTGVIEAFGQAALYEEALVAFNT MHEVGSNPSIETFHSLLYSFARGGLFKESEVILSRLVNSGIPRNRDTFNATIEAYKQGGKFEEAVKTYVDMEKSRCDPDE RTLEAVLSVYSCARLVDECREQFEEMKASDILPSIMCYCMMLSVYGKTESWDDVNELLEEMLSNRVSNIHQVIGQMIKGN YDDDSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGIFPELFRKNKLVWSVDVHRMSEGG MYTALSVWLNDLSDILVKGQDLPQLAVVVSVRGQLEKSSAARESPITRAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLK QLLSTQEPTSEESPKSNIVALTNSPIFAAGTRTSTSSDTNHSGGNPSQRKTKMKKELAGSPA,Full sequence: MNLAIPNPNSHHLSFLIQNSTFIGNRRFANSNHLSFLSGGKRPCSVAKINAKTKDLVLGNPSVSVEKGKYSYDVESLINK LSSLPPRGSIARCLDIFKNKLSLNDFALVFKEFAGRGDWQRSLRLFKYMQRQIWCKPNEHIYTIMISLLGREGLLDKCLE VFEEMPSQGVARSVFSYTALINAYGRNGRYETSLELLDRMKSEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEG IQPDIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLGRLEKVSDLLSEMASGGSLPDITSYNV LLEAYAKSGSIKEAMGVFHQMQAAGCSPNANTYSVLLNLFGQNGRYDDVRQLFLEMKSSNTDPDAATYNILIDVFGEGGY FKEVVTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTAKDVVPSSKAYTGVIEAFGQAALYEEALVAFNT MHEVGSNPSIETFHSLLYSFARGGLFKESEVILSRLVNSGIPRNRDTFNATIEAYKQGGKFEEAVKTYVDMEKSRCDPDE RTLEAVLSVYSCARLVDECREQFEEMKASDILPSIMCYCMMLSVYGKTESWDDVNELLEEMLSNRVSNIHQVIGQMIKGN YDDDSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGIFPELFRKNKLVWSVDVHRMSEGG MYTALSVWLNDLSDILVKGQDLPQLAVVVSVRGQLEKSSAARESPITRAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLK QLLSTQEPTSEESPKSNIVALTNSPIFAAGTRTSTSSDTNHSGGNPSQRKTKMKKELAGSPA,Full sequence: MNLAIPNPNSHHLSFLIQNSTFIGNRRFANSNHLSFLSGGKRPCSVAKINAKTKDLVLGNPSVSVEKGKYSYDVESLINK LSSLPPRGSIARCLDIFKNKLSLNDFALVFKEFAGRGDWQRSLRLFKYMQRQIWCKPNEHIYTIMISLLGREGLLDKCLE VFEEMPSQGVARSVFSYTALINAYGRNGRYETSLELLDRMKSEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEG IQPDIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLGRLEKVSDLLSEMASGGSLPDITSYNV LLEAYAKSGSIKEAMGVFHQMQAAGCSPNANTYSVLLNLFGQNGRYDDVRQLFLEMKSSNTDPDAATYNILIDVFGEGGY FKEVVTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTAKDVVPSSKAYTGVIEAFGQAALYEEALVAFNT MHEVGSNPSIETFHSLLYSFARGGLFKESEVILSRLVNSGIPRNRDTFNATIEAYKQGGKFEEAVKTYVDMEKSRCDPDE RTLEAVLSVYSCARLVDECREQFEEMKASDILPSIMCYCMMLSVYGKTESWDDVNELLEEMLSNRVSNIHQVIGQMIKGN YDDDSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGIFPELFRKNKLVWSVDVHRMSEGG MYTALSVWLNDLSDILVKGQDLPQLAVVVSVRGQLEKSSAARESPITRAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLK QLLSTQEPTSEESPKSNIVALTNSPIFAAGTRTSTSSDTNHSGGNPSQRKTKMKKELAGSPA,Full sequence: MNLAIPNPNSHHLSFLIQNSTFIGNRRFANSNHLSFLSGGKRPCSVAKINAKTKDLVLGNPSVSVEKGKYSYDVESLINK LSSLPPRGSIARCLDIFKNKLSLNDFALVFKEFAGRGDWQRSLRLFKYMQRQIWCKPNEHIYTIMISLLGREGLLDKCLE VFEEMPSQGVARSVFSYTALINAYGRNGRYETSLELLDRMKSEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEG IQPDIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLGRLEKVSDLLSEMASGGSLPDITSYNV LLEAYAKSGSIKEAMGVFHQMQAAGCSPNANTYSVLLNLFGQNGRYDDVRQLFLEMKSSNTDPDAATYNILIDVFGEGGY FKEVVTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTAKDVVPSSKAYTGVIEAFGQAALYEEALVAFNT MHEVGSNPSIETFHSLLYSFARGGLFKESEVILSRLVNSGIPRNRDTFNATIEAYKQGGKFEEAVKTYVDMEKSRCDPDE RTLEAVLSVYSCARLVDECREQFEEMKASDILPSIMCYCMMLSVYGKTESWDDVNELLEEMLSNRVSNIHQVIGQMIKGN YDDDSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGIFPELFRKNKLVWSVDVHRMSEGG MYTALSVWLNDLSDILVKGQDLPQLAVVVSVRGQLEKSSAARESPITRAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLK QLLSTQEPTSEESPKSNIVALTNSPIFAAGTRTSTSSDTNHSGGNPSQRKTKMKKELAGSPA,Full sequence: MNLAIPNPNSHHLSFLIQNSTFIGNRRFANSNHLSFLSGGKRPCSVAKINAKTKDLVLGNPSVSVEKGKYSYDVESLINK LSSLPPRGSIARCLDIFKNKLSLNDFALVFKEFAGRGDWQRSLRLFKYMQRQIWCKPNEHIYTIMISLLGREGLLDKCLE VFEEMPSQGVARSVFSYTALINAYGRNGRYETSLELLDRMKSEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEG IQPDIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLGRLEKVSDLLSEMASGGSLPDITSYNV LLEAYAKSGSIKEAMGVFHQMQAAGCSPNANTYSVLLNLFGQNGRYDDVRQLFLEMKSSNTDPDAATYNILIDVFGEGGY FKEVVTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTAKDVVPSSKAYTGVIEAFGQAALYEEALVAFNT MHEVGSNPSIETFHSLLYSFARGGLFKESEVILSRLVNSGIPRNRDTFNATIEAYKQGGKFEEAVKTYVDMEKSRCDPDE RTLEAVLSVYSCARLVDECREQFEEMKASDILPSIMCYCMMLSVYGKTESWDDVNELLEEMLSNRVSNIHQVIGQMIKGN YDDDSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGIFPELFRKNKLVWSVDVHRMSEGG MYTALSVWLNDLSDILVKGQDLPQLAVVVSVRGQLEKSSAARESPITRAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLK QLLSTQEPTSEESPKSNIVALTNSPIFAAGTRTSTSSDTNHSGGNPSQRKTKMKKELAGSPA,Full sequence: MNLAIPNPNSHHLSFLIQNSTFIGNRRFANSNHLSFLSGGKRPCSVAKINAKTKDLVLGNPSVSVEKGKYSYDVESLINK LSSLPPRGSIARCLDIFKNKLSLNDFALVFKEFAGRGDWQRSLRLFKYMQRQIWCKPNEHIYTIMISLLGREGLLDKCLE VFEEMPSQGVARSVFSYTALINAYGRNGRYETSLELLDRMKSEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEG IQPDIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLGRLEKVSDLLSEMASGGSLPDITSYNV LLEAYAKSGSIKEAMGVFHQMQAAGCSPNANTYSVLLNLFGQNGRYDDVRQLFLEMKSSNTDPDAATYNILIDVFGEGGY FKEVVTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTAKDVVPSSKAYTGVIEAFGQAALYEEALVAFNT MHEVGSNPSIETFHSLLYSFARGGLFKESEVILSRLVNSGIPRNRDTFNATIEAYKQGGKFEEAVKTYVDMEKSRCDPDE RTLEAVLSVYSCARLVDECREQFEEMKASDILPSIMCYCMMLSVYGKTESWDDVNELLEEMLSNRVSNIHQVIGQMIKGN YDDDSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGIFPELFRKNKLVWSVDVHRMSEGG MYTALSVWLNDLSDILVKGQDLPQLAVVVSVRGQLEKSSAARESPITRAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLK QLLSTQEPTSEESPKSNIVALTNSPIFAAGTRTSTSSDTNHSGGNPSQRKTKMKKELAGSPA,Full sequence: MNLAIPNPNSHHLSFLIQNSTFIGNRRFANSNHLSFLSGGKRPCSVAKINAKTKDLVLGNPSVSVEKGKYSYDVESLINK LSSLPPRGSIARCLDIFKNKLSLNDFALVFKEFAGRGDWQRSLRLFKYMQRQIWCKPNEHIYTIMISLLGREGLLDKCLE VFEEMPSQGVARSVFSYTALINAYGRNGRYETSLELLDRMKSEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEG IQPDIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLGRLEKVSDLLSEMASGGSLPDITSYNV LLEAYAKSGSIKEAMGVFHQMQAAGCSPNANTYSVLLNLFGQNGRYDDVRQLFLEMKSSNTDPDAATYNILIDVFGEGGY FKEVVTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTAKDVVPSSKAYTGVIEAFGQAALYEEALVAFNT MHEVGSNPSIETFHSLLYSFARGGLFKESEVILSRLVNSGIPRNRDTFNATIEAYKQGGKFEEAVKTYVDMEKSRCDPDE RTLEAVLSVYSCARLVDECREQFEEMKASDILPSIMCYCMMLSVYGKTESWDDVNELLEEMLSNRVSNIHQVIGQMIKGN YDDDSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGIFPELFRKNKLVWSVDVHRMSEGG MYTALSVWLNDLSDILVKGQDLPQLAVVVSVRGQLEKSSAARESPITRAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLK QLLSTQEPTSEESPKSNIVALTNSPIFAAGTRTSTSSDTNHSGGNPSQRKTKMKKELAGSPA,Full sequence: MNLAIPNPNSHHLSFLIQNSTFIGNRRFANSNHLSFLSGGKRPCSVAKINAKTKDLVLGNPSVSVEKGKYSYDVESLINK LSSLPPRGSIARCLDIFKNKLSLNDFALVFKEFAGRGDWQRSLRLFKYMQRQIWCKPNEHIYTIMISLLGREGLLDKCLE VFEEMPSQGVARSVFSYTALINAYGRNGRYETSLELLDRMKSEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEG IQPDIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLGRLEKVSDLLSEMASGGSLPDITSYNV LLEAYAKSGSIKEAMGVFHQMQAAGCSPNANTYSVLLNLFGQNGRYDDVRQLFLEMKSSNTDPDAATYNILIDVFGEGGY FKEVVTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTAKDVVPSSKAYTGVIEAFGQAALYEEALVAFNT MHEVGSNPSIETFHSLLYSFARGGLFKESEVILSRLVNSGIPRNRDTFNATIEAYKQGGKFEEAVKTYVDMEKSRCDPDE RTLEAVLSVYSCARLVDECREQFEEMKASDILPSIMCYCMMLSVYGKTESWDDVNELLEEMLSNRVSNIHQVIGQMIKGN YDDDSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGIFPELFRKNKLVWSVDVHRMSEGG MYTALSVWLNDLSDILVKGQDLPQLAVVVSVRGQLEKSSAARESPITRAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLK QLLSTQEPTSEESPKSNIVALTNSPIFAAGTRTSTSSDTNHSGGNPSQRKTKMKKELAGSPA,Full sequence: MNLAIPNPNSHHLSFLIQNSTFIGNRRFANSNHLSFLSGGKRPCSVAKINAKTKDLVLGNPSVSVEKGKYSYDVESLINK LSSLPPRGSIARCLDIFKNKLSLNDFALVFKEFAGRGDWQRSLRLFKYMQRQIWCKPNEHIYTIMISLLGREGLLDKCLE VFEEMPSQGVARSVFSYTALINAYGRNGRYETSLELLDRMKSEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEG IQPDIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLGRLEKVSDLLSEMASGGSLPDITSYNV LLEAYAKSGSIKEAMGVFHQMQAAGCSPNANTYSVLLNLFGQNGRYDDVRQLFLEMKSSNTDPDAATYNILIDVFGEGGY FKEVVTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTAKDVVPSSKAYTGVIEAFGQAALYEEALVAFNT MHEVGSNPSIETFHSLLYSFARGGLFKESEVILSRLVNSGIPRNRDTFNATIEAYKQGGKFEEAVKTYVDMEKSRCDPDE RTLEAVLSVYSCARLVDECREQFEEMKASDILPSIMCYCMMLSVYGKTESWDDVNELLEEMLSNRVSNIHQVIGQMIKGN YDDDSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGIFPELFRKNKLVWSVDVHRMSEGG MYTALSVWLNDLSDILVKGQDLPQLAVVVSVRGQLEKSSAARESPITRAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLK QLLSTQEPTSEESPKSNIVALTNSPIFAAGTRTSTSSDTNHSGGNPSQRKTKMKKELAGSPA,Full sequence: MNLAIPNPNSHHLSFLIQNSTFIGNRRFANSNHLSFLSGGKRPCSVAKINAKTKDLVLGNPSVSVEKGKYSYDVESLINK LSSLPPRGSIARCLDIFKNKLSLNDFALVFKEFAGRGDWQRSLRLFKYMQRQIWCKPNEHIYTIMISLLGREGLLDKCLE VFEEMPSQGVARSVFSYTALINAYGRNGRYETSLELLDRMKSEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEG IQPDIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLGRLEKVSDLLSEMASGGSLPDITSYNV LLEAYAKSGSIKEAMGVFHQMQAAGCSPNANTYSVLLNLFGQNGRYDDVRQLFLEMKSSNTDPDAATYNILIDVFGEGGY FKEVVTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTAKDVVPSSKAYTGVIEAFGQAALYEEALVAFNT MHEVGSNPSIETFHSLLYSFARGGLFKESEVILSRLVNSGIPRNRDTFNATIEAYKQGGKFEEAVKTYVDMEKSRCDPDE RTLEAVLSVYSCARLVDECREQFEEMKASDILPSIMCYCMMLSVYGKTESWDDVNELLEEMLSNRVSNIHQVIGQMIKGN YDDDSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGIFPELFRKNKLVWSVDVHRMSEGG MYTALSVWLNDLSDILVKGQDLPQLAVVVSVRGQLEKSSAARESPITRAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLK QLLSTQEPTSEESPKSNIVALTNSPIFAAGTRTSTSSDTNHSGGNPSQRKTKMKKELAGSPA,Full sequence: MNLAIPNPNSHHLSFLIQNSTFIGNRRFANSNHLSFLSGGKRPCSVAKINAKTKDLVLGNPSVSVEKGKYSYDVESLINK LSSLPPRGSIARCLDIFKNKLSLNDFALVFKEFAGRGDWQRSLRLFKYMQRQIWCKPNEHIYTIMISLLGREGLLDKCLE VFEEMPSQGVARSVFSYTALINAYGRNGRYETSLELLDRMKSEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEG IQPDIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLGRLEKVSDLLSEMASGGSLPDITSYNV LLEAYAKSGSIKEAMGVFHQMQAAGCSPNANTYSVLLNLFGQNGRYDDVRQLFLEMKSSNTDPDAATYNILIDVFGEGGY FKEVVTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTAKDVVPSSKAYTGVIEAFGQAALYEEALVAFNT MHEVGSNPSIETFHSLLYSFARGGLFKESEVILSRLVNSGIPRNRDTFNATIEAYKQGGKFEEAVKTYVDMEKSRCDPDE RTLEAVLSVYSCARLVDECREQFEEMKASDILPSIMCYCMMLSVYGKTESWDDVNELLEEMLSNRVSNIHQVIGQMIKGN YDDDSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGIFPELFRKNKLVWSVDVHRMSEGG MYTALSVWLNDLSDILVKGQDLPQLAVVVSVRGQLEKSSAARESPITRAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLK QLLSTQEPTSEESPKSNIVALTNSPIFAAGTRTSTSSDTNHSGGNPSQRKTKMKKELAGSPA,Full sequence: MNLAIPNPNSHHLSFLIQNSTFIGNRRFANSNHLSFLSGGKRPCSVAKINAKTKDLVLGNPSVSVEKGKYSYDVESLINK LSSLPPRGSIARCLDIFKNKLSLNDFALVFKEFAGRGDWQRSLRLFKYMQRQIWCKPNEHIYTIMISLLGREGLLDKCLE VFEEMPSQGVARSVFSYTALINAYGRNGRYETSLELLDRMKSEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEG IQPDIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLGRLEKVSDLLSEMASGGSLPDITSYNV LLEAYAKSGSIKEAMGVFHQMQAAGCSPNANTYSVLLNLFGQNGRYDDVRQLFLEMKSSNTDPDAATYNILIDVFGEGGY FKEVVTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTAKDVVPSSKAYTGVIEAFGQAALYEEALVAFNT MHEVGSNPSIETFHSLLYSFARGGLFKESEVILSRLVNSGIPRNRDTFNATIEAYKQGGKFEEAVKTYVDMEKSRCDPDE RTLEAVLSVYSCARLVDECREQFEEMKASDILPSIMCYCMMLSVYGKTESWDDVNELLEEMLSNRVSNIHQVIGQMIKGN YDDDSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGIFPELFRKNKLVWSVDVHRMSEGG MYTALSVWLNDLSDILVKGQDLPQLAVVVSVRGQLEKSSAARESPITRAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLK QLLSTQEPTSEESPKSNIVALTNSPIFAAGTRTSTSSDTNHSGGNPSQRKTKMKKELAGSPA,Full sequence: MNLAIPNPNSHHLSFLIQNSTFIGNRRFANSNHLSFLSGGKRPCSVAKINAKTKDLVLGNPSVSVEKGKYSYDVESLINK LSSLPPRGSIARCLDIFKNKLSLNDFALVFKEFAGRGDWQRSLRLFKYMQRQIWCKPNEHIYTIMISLLGREGLLDKCLE VFEEMPSQGVARSVFSYTALINAYGRNGRYETSLELLDRMKSEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEG IQPDIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLGRLEKVSDLLSEMASGGSLPDITSYNV LLEAYAKSGSIKEAMGVFHQMQAAGCSPNANTYSVLLNLFGQNGRYDDVRQLFLEMKSSNTDPDAATYNILIDVFGEGGY FKEVVTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTAKDVVPSSKAYTGVIEAFGQAALYEEALVAFNT MHEVGSNPSIETFHSLLYSFARGGLFKESEVILSRLVNSGIPRNRDTFNATIEAYKQGGKFEEAVKTYVDMEKSRCDPDE RTLEAVLSVYSCARLVDECREQFEEMKASDILPSIMCYCMMLSVYGKTESWDDVNELLEEMLSNRVSNIHQVIGQMIKGN YDDDSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGIFPELFRKNKLVWSVDVHRMSEGG MYTALSVWLNDLSDILVKGQDLPQLAVVVSVRGQLEKSSAARESPITRAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLK QLLSTQEPTSEESPKSNIVALTNSPIFAAGTRTSTSSDTNHSGGNPSQRKTKMKKELAGSPA,Full sequence: MNLAIPNPNSHHLSFLIQNSTFIGNRRFANSNHLSFLSGGKRPCSVAKINAKTKDLVLGNPSVSVEKGKYSYDVESLINK LSSLPPRGSIARCLDIFKNKLSLNDFALVFKEFAGRGDWQRSLRLFKYMQRQIWCKPNEHIYTIMISLLGREGLLDKCLE VFEEMPSQGVARSVFSYTALINAYGRNGRYETSLELLDRMKSEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEG IQPDIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLGRLEKVSDLLSEMASGGSLPDITSYNV LLEAYAKSGSIKEAMGVFHQMQAAGCSPNANTYSVLLNLFGQNGRYDDVRQLFLEMKSSNTDPDAATYNILIDVFGEGGY FKEVVTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTAKDVVPSSKAYTGVIEAFGQAALYEEALVAFNT MHEVGSNPSIETFHSLLYSFARGGLFKESEVILSRLVNSGIPRNRDTFNATIEAYKQGGKFEEAVKTYVDMEKSRCDPDE RTLEAVLSVYSCARLVDECREQFEEMKASDILPSIMCYCMMLSVYGKTESWDDVNELLEEMLSNRVSNIHQVIGQMIKGN YDDDSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGIFPELFRKNKLVWSVDVHRMSEGG MYTALSVWLNDLSDILVKGQDLPQLAVVVSVRGQLEKSSAARESPITRAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLK QLLSTQEPTSEESPKSNIVALTNSPIFAAGTRTSTSSDTNHSGGNPSQRKTKMKKELAGSPA; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 122.664609 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)MNLAIPNPN SHHLSFLIQN STFIGNRRFA NSNHLSFLSG G KRPCSVAK INAKTKDLVL GNPSVSVEKG KYSYDVESLI NKLSSLPPRG SIARCLDIFK NKLSLNDFAL VFKEFAGRGD WQ RSLRLFK YMQRQIWCKP NEHIYTIMIS LLGREGLLDK CLEVFEEMPS QGVARSVFSY TALINAYGRN GRYETSLELL DRM KSEKIS PSILTYNTVI NACARGGLDW EGLLGLFAEM RHEGIQPDIV TYNTLLSACA IRGLGDEAEM VFRTMNDGGI VPDL TTYSH LVETFGKLGR LEKVSDLLSE MASGGSLPDI TSYNVLLEAY AKSGSIKEAM GVFHQMQAAG CSPNANTYSV LLNLF GQNG RYDDVRQLFL EMKSSNTDPD AATYNILIDV FGEGGYFKEV VTLFHDMVEE NIEPDMETYE GIIFACGKGG LHEDAR KIL QYMTAKDVVP SSKAYTGVIE AFGQAALYEE ALVAFNTMHE VGSNPSIETF HSLLYSFARG GLFKESEVIL SRLVNSG IP RNRDTFNATI EAYKQGGKFE EAVKTYVDME KSRCDPDERT LEAVLSVYSC ARLVDECREQ FEEMKASDIL PSIMCYCM M LSVYGKTESW DDVNELLEEM LSNRVSNIHQ VIGQMIKGNY DDDSNWQIVE YVLDKLNSEG CGLGIRFYNA LLDALWWLG QKERAARVLN EATKRGIFPE LFRKNKLVWS VDVHRMSEGG MYTALSVWLN DLSDILVKGQ DLPQLAVVVS VRGQLEKSSA ARESPITRA AFSFLQDHVS SSFSFTGWNG GRIMCQRSQL KQLLSTQEPT SEESPKSNIV ALTNSPIFAA GTRTSTSSDT N HSGGNPSQ RKTKMKKELA GSPA

Macromolecule #7: PAP3, pTAC10

• Macromolecule: Name: PAP3, pTAC10 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 79.815469 KDa

Sequence

String:

MQICQATLTT FTFTNPSNPN FCKPKPLFPS FQPPRRVTLP PCRGFSSDEF PVDETFLEKF GPKDKDTEDE ARRRNWIERG WAPWEEILT PEADFARKSL NEGEEVPLQS PEAIEAFKML RPSYRKKKIK EMGITEDEWY AKQFEIRGDK PPPLDTSWAG P LVVRQIPP RDWPPKGWEV DRKELEFIRE AHKLMAERVW LEDLDKDLKV GEDATVDKMC LERFKVFLKQ YNEWVEANKD RL EEDSYKY DQDFYPGRRI RGKDYKEGMY ELPFYYPGMI CEGTVTTLHL YQGAFVDIGG VHEGWVPIKG NDWFWIRHFI RVG MHVIVE ITAKRDPYRF RFPLELRFVH PNIDHMIFNK FDFPPIFHRD GDTNPDEIRR DCGRPPEPRK DPGSKPEEEG LLSD HPYVD KLWQLHVAEQ MILDDYEANP EKYKGKKLSE LSDDEGFDER KEIEHGEAYY KKTKLPKVIL KTSVKELDLE AALIE RKYH NKLMMEAKAR GEGYKIEKLR RNIEMDEYDS LHWRRSLEER EALLRDISSR QALGLPLEEP GRYKPGSFFG KDQYDP TSA LYQYDYWGEP KNSEISKQER MKDAHNKSIV GKGNVWYDMS YDDAIKQTIE RRKAESNVVT QKEEETESKE EEEDDDD EY EFDDFDYSIL SDESSIGYSE QQPLVNGTQV FTD

Macromolecule #8: PAP4, FSD3

• Macromolecule: Name: PAP4, FSD3 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 30.418436 KDa

Sequence

String:

MASCVVTTSS FCTLSDSNIR LKSSKLVHLS NQQRRRFPLG SRGGLKVEAY YGLKTPPYPL DALEPYMSRR TLEVHWGKHH RGYIDNLNK QLGKDDKLYG YTMEELIKAT YNNGNPLPEF NNAAQVYNHD FFWESMQPGG GDMPQKGVLE QIEKDFGSFT N FREKFTNA ALTQFGSGWV WLVLKREERR LEVVKTSNAI NPLVWDDIPI ISLDVWEHSY YLDYKNERGK YINTFLNHLV SW NAAMSRM ARAEAFVNLG EPNIPIA

Macromolecule #9: PAP5, pTAC12, HEMERA

• Macromolecule: Name: PAP5, pTAC12, HEMERA / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 60.884246 KDa

Sequence

String:

MASISTTSWL YRDKLCTESG KLGTCILQRP VKCGFPVKRL YVGITSKDVL MRDCIKCKKD DDDDDASEGS SKKDGQGYEY VSVERAPYY SYMDSTSGKM EPASGARASI PGEDYWPEGT SSRVRAARAP QPAGESSSFP SYGKNPGSRR KKNRKATEGN A AVETYDEV SDSEDSSEEE ESDSSNGFVV YNNEVEGEDE EETGFELDKK LGRPHPFIDP TKKKQIETTL TSDESWWNWR KP EKEQWSR WQRRRPDVET VFLKAMAETG QVKLYGKEPT LTETSLYRAR RHLFKEERLQ AERERLAKEG PMAFYSEWVK AWK RDTSRE AVQKHFEETG EDENTQLIEM FSHQTDREYR IMMGTDVRIK RDPLAMRMKE DQIKQIWGGD PVYPTINYIQ APDA VMDFR GPDFHEPTPN MLSYLKENCK VISREMHETL LAKEKTEQVE VPDIDDAMAQ AVDIGENDDE EEDTEEAEKD EKVAR NWSV LKSTPELRNS KPKPKKEGRM SLDEAVDDSE NLTDFLMDFD EETDP

Macromolecule #10: PAP6, FLN1

• Macromolecule: Name: PAP6, FLN1 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 52.435281 KDa

Sequence

String:

MASLHLFPHL HHFDSIIHRR EIAAHSRRQF LSPKASINGG VVTNGASAAE TAKPSRKGRN KKKQTEETNP DSDPALVDYD DGIDFPYDD PPLVCCFGAV QKEFVPVVRV HDNPMHPDIY SQWKMLQWDP PEFGRAPGGP PSNVAISHVR LGGRAAFMGK V GGDDYGEE LVLMMNKERV QTRGVKFDEG ASTACTRVKI KFEDGKMKAE TVKEPPEDSL LASELNLAVL KEARIFHFNS EV LTSPTME STLFKAIQWS KKFGGLIFFD LNLPLPLWRS RNETRKLIKK SWDEANIIEV SQQELEFLLD EEYYERRRNY TPQ YFAEDF EQTKNRRDYY HYTPEEIKPL WHDDLKLLVV TDGTLRLHYY TPKFDGVVVG TEDVLITPFT CDRTGSGDAV VAGI MRKLT TCPEMFEDQD VLERQLRFAV AAGIISQWTI GAVRGFPTES ATQNLKEQVY VPSMW

Macromolecule #11: PAP7, pTAC14

• Macromolecule: Name: PAP7, pTAC14 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 55.675461 KDa

Sequence

String:

MASPVSLHYL INTFISKPQG FCNGTVSSPR PRSSFVRERQ NSVKPIKVAS LETQPFPLFQ SPASEESSSS ELESADPDFY KIGYVRRVR AYGVEFKEGP DGFGIYASKD IEPRRRARVI MEIPHELMIT IRQKHPWMFF PDIVPIGHPI FDIINSTDPE R DWDLRLAC LLLFSFDRED HFWRLYGDFL PAADECSSLL LATEEDLAEL QDPQLVSTIR QQQKRVLEFW EKNWHSGVPL KI KRLAEDA ERFIWAVSIA QTRCISMKTR IGALVQDLNM MIPYADMLNH SFEPNCFLHW RPKDRILEVM SNAGQAIKKG EEM TINYMP GQKNNMLMER YGFSTPVNPW DAIPFSGDSR IHLNSFLSVF NIFGLPEEYY HDSELSGDDS FVDGAVIAAA RTLP TWSDI DLPPIPSAER KAVKELQDEC RKMLAEYPTT SEQDQKLLDS LSEARTTFAT AVKYRMHRKM FIGKIIKALD IYQER LLF

Macromolecule #12: PAP8, pTAC6

• Macromolecule: Name: PAP8, pTAC6 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 38.039504 KDa

Sequence

String:

MASSAASPSL SLLSLTPKPP PSPSTASATS HRLFPSFRTN GCFAPLTLKP RRGRSIIVKV DDGDADGGGQ DEYDMDDEEV EEVDNKKDY DVEYDPLAAA MAAASGGGGD GDIAFVQSKS FISTQGWDSE MVVDYRINED EFHKISLLDC DFFIRKPPDP D NDVYDFRE MYVTPPDTDI YSVPRVLAPM PQKYIRCAMS DYGCYDVTEP PIDAPRDPLY KSEREISKVF LTKHYRNRRL ND PEFVLDF EEIYVIDSKT KSITRARVLV TVPGGRKRDR KDDLLVIRDN GNSFKIIHVG ERDDPTTVIE REEWTKTRED MEK HLRKLR DFSVSNWF

Macromolecule #13: PAP9, FSD2

• Macromolecule: Name: PAP9, FSD2 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 34.008883 KDa

Sequence

String:

MMTSTCSFLS SCSLLPSQEP NQRMQWKRHE KRQFSRKVAV SGVIRAGFEL KPPPYPLDAL EPHMSRETLD YHWGKHHKTY VENLNKQIV GTDLDGLSLE EVVLLSYNRG NMLPAFNNAA QAWNHEFFWE SIQPGGGGKP SGDLLRLIER DFGSFSDFVE R FKAAAASN FGSGWTWLAY KANRLDVANA VNPLPKEEDK KLVIVKTPNA VNPLVWDYSP LLTIDTWEHA YYLDFENRRI EY INTFMEK LVSWETVSTR LESAMARAAQ REQEGSDTEA EENPDDEEPE VYLDDASEVD

Macromolecule #14: PAP10, TRXz

• Macromolecule: Name: PAP10, TRXz / type: protein_or_peptide / ID: 14 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 20.954404 KDa

Sequence

String:

MALVQSRALP RLNVSLSPIL STLHAPPSSL FLRREIRPVV TSPFSSSTTG NLPFSPLTHP RKILCPPPRG KFVREDYLVR KLSAQELQD LVKGERKVPL IVDFYATWCG PCILMAQELE MLAVEYESNA MIVKVDTDDE YEFARDMQVR GLPTLFFISP D PSKDAIRT EGLIPLQMMR DIIDNDM

Macromolecule #15: PAP11, MurE-like

• Macromolecule: Name: PAP11, MurE-like / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 85.12168 KDa

Sequence

String:

MAFTFFSPHP VFLPLGRTTS SFSYKPVYSP FPRNSRNLQL AAGPARRNSY PNPADDDPPE APEDSMHGVS KFQQIQRQAA RARKLEEED FEKNRNTYLS AIADVEDAPE TGRDDVESGG DLFSDIDRAI SMKRSEFVKK GLLQPNPPKT TSSKKLDEEE D DVTDVVDE LDEEEVVDLD EIDKLTGLTE VSDEEDWVDE EGNPRINKKK ETGSDHHFEF DLDDFGGSKA RIVEPKFRLS LA ELLDESK VVPISVYGDL DVEITGIQHD SRGVSSGDLF VCCENEGDSV LSEADKRGAV AVVASKEIDI EDTLGCRALV IVE DTEAVL AALASSFYRH PSKNMAVIGV TGTNGKTTTT YLIKSLYEAM GVRTGMFSTV SCYVHGDNKM DSPTTTSPDA VLVQ SMMAK MLHNGTEALV MEASPQELAS GKCDEVDFDI AVFTNLTRED SDFRGTDEEY RDAEAKLFAR MVDPERHRKV VNIDD PNAA FFVQQGNPDV PVVTFAMENT KADVHPLKFE LSLFETQVLV NTPQGILEIS SGLLGRHNIY NILAAVAVGI AVGAPL EDI VRGVEEVDAV PGRCELIDEE QAFGVIVDHA NTPDGLSRLL DSVRELKPRR IITVIGCAGE NERGKRPVMT KIATEKS DV TMLTSDNPGN EDPLDILDDM LSGIGWTMQE YLKHGEHDYY PPLSNGHRLF LHDVRRVAVR CAVAMGEEGD MVVVAGKG H ESYQLEGDKK EFYDDREECR EALQYVDELH QAGIDTSEFP WRLPESH

Macromolecule #16: PAP12, pTAC7

• Macromolecule: Name: PAP12, pTAC7 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 18.835195 KDa

Sequence

String:

MFCSSFTSSI SRIGDARSGN SRASSFTFQT QVSCGIQRDD NGRRIWRRRT LTKKDDMLRY KLQRVPFVEE QVRKIKEVGK VMTMDIERL LLSEDNRFEF VNSVAAEATE YVEKNRDEYG GTKKAIFHVL SNRVNDLGFD RPEAYAESDP YKPGPGYLKE Y YT

Macromolecule #17: FLN2

• Macromolecule: Name: FLN2 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 68.527102 KDa

Sequence

String:

MASLSFTQFL PFPRCSVDVP CLQPHGFVKF RGERWKGKHS FLMVAGRRKL SESAPLDEDD GGNGAVGGKK PTKVPKKSGA RTAKKKVVA KDEPLEESSQ LLVDSDNVSD NESDTKEPVR RTRKKAAASS DVNEGKTEKK VRRKRTVKKD KEVEDGLVTY D EASDVEEA LTVEATDADS EGEEIDLSKH ESEDISHTYG WPPLVCCFGS AQHAFVPSGR PANRLLDYER QERMKDAVWA PE KYIRAPG GCAGGVAIAL ASLGGKAAFM GKLGDDDFGQ AMLYYLNVCQ VQTRSVKIDS KRVTACSTMK ISKRGRLKST CVK PCAEDS LSKSEINVDV LKEAKMFYFT THSLLDKKMM STTLQAIKIS KQLGNVIFYD LNLPLPLWQS LEETKSLIQE VWDL ADVIE VTKQELEFLC GIEPTEEFDT KNNDSSKFVH YEPETVEPLW HENLKILFVT NGTSKIHYYT KEHNGAVLGM EDVPI TPFT RDMSASGDGI VAGLIRMLTV QPDLMNDKGY LERTARYAIE CGVVDQWLLA QTRGYPPKDD MEEEEDDDEE EEMESD PNG IRSITEREYR TSKPYDEPDG PYVMKPVEER EYRKLELVGS MGEDDDSS

Macromolecule #18: pTAC18

• Macromolecule: Name: pTAC18 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 16.430916 KDa

Sequence

String:

MASLIMTTPF SGSLTQCKKK TNNLSVNRAF FKVTCSMQTP LEELYNVKVE RKVSQRRLDE LGVSRWSVWK TGKCKLPWDW QVDQLVYIE EGEVRVVPEG SKRFMQFLAG DLVRYPKWLE ADLFFNAPYS ERYCFKAYAD D

Macromolecule #19: PRIN2, PLASTID REDOX INSENSITIVE 2

• Macromolecule: Name: PRIN2, PLASTID REDOX INSENSITIVE 2 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sinapis alba (white mustard)
• Molecular weight: Theoretical: 20.915762 KDa

Sequence

String:

MQMASLDAAL FSSRLQFHSS SSSSSSIRCA LPTIFNLSSP AGSSCVLKST QFLKRRLRAK STNFSLSSLP RRGFVCRAAE YKFPDPIPE FAEAETKKFR DHMAKKLAKR DLFEDSVDEI VGVCTEIFET FLRNEYGGPG TLLVIPFIDM ADTLNERELP G GPQAARAA IKWAQDHVDK DWKEWTGTD

Macromolecule #20: 2,3-DIHYDROXY-1,4-DITHIOBUTANE

• Macromolecule: Name: 2,3-DIHYDROXY-1,4-DITHIOBUTANE / type: ligand / ID: 20 / Number of copies: 1 / Formula: DTT
• Molecular weight: Theoretical: 154.251 Da

Chemical component information

ChemComp-DTT:
2,3-DIHYDROXY-1,4-DITHIOBUTANE

Macromolecule #21: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 21 / Number of copies: 1 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #22: FE (III) ION

• Macromolecule: Name: FE (III) ION / type: ligand / ID: 22 / Number of copies: 2 / Formula: FE
• Molecular weight: Theoretical: 55.845 Da

Macromolecule #23: CALCIUM ION

• Macromolecule: Name: CALCIUM ION / type: ligand / ID: 23 / Number of copies: 2 / Formula: CA
• Molecular weight: Theoretical: 40.078 Da

Macromolecule #24: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 24 / Number of copies: 1 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Macromolecule #25: S-ADENOSYL-L-HOMOCYSTEINE

• Macromolecule: Name: S-ADENOSYL-L-HOMOCYSTEINE / type: ligand / ID: 25 / Number of copies: 1 / Formula: SAH
• Molecular weight: Theoretical: 384.411 Da

Chemical component information

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.6
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 51.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.4 µm / Nominal defocus min: 1.0 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION

Startup model

Type of model: EMDB MAP
EMDB ID:

14571

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 105796
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD