Biotechnology and Biological Sciences Research Council (BBSRC)
BB/T008741/1
United Kingdom
Citation
Journal: Biochem J / Year: 2024 Title: The consequence of ATP synthase dimer angle on mitochondrial morphology studied by cryo-electron tomography. Authors: Emma Buzzard / Mathew McLaren / Piotr Bragoszewski / Andrea Brancaccio / Holly Ford / Bertram Daum / Patricia Kuwabara / Ian Collinson / Vicki Gold / Abstract: Mitochondrial ATP synthases form rows of dimers, which induce membrane curvature to give cristae their characteristic lamellar or tubular morphology. The angle formed between the central stalks of ...Mitochondrial ATP synthases form rows of dimers, which induce membrane curvature to give cristae their characteristic lamellar or tubular morphology. The angle formed between the central stalks of ATP synthase dimers varies between species. Using cryo-electron tomography and sub-tomogram averaging, we determined the structure of the ATP synthase dimer from the nematode worm C. elegans and show that the dimer angle differs from previously determined structures. The consequences of this species-specific difference at the dimer interface were investigated by comparing C. elegans and S. cerevisiae mitochondrial morphology. We reveal that C. elegans has a larger ATP synthase dimer angle with more lamellar (flatter) cristae when compared to yeast. The underlying cause of this difference was investigated by generating an atomic model of the C. elegans ATP synthase dimer by homology modelling. A comparison of our C. elegans model to an existing S. cerevisiae structure reveals the presence of extensions and rearrangements in C. elegans subunits associated with maintaining the dimer interface. We speculate that increasing dimer angles could provide an advantage for species that inhabit variable-oxygen environments by forming flatter more energetically efficient cristae.
Model: UltrAuFoil R2/2 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 60 sec. / Pretreatment - Atmosphere: AIR
Vitrification
Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV
Details
Mitochondria isolated from C. elegans underwent 2-3 freeze-thaw cycles, to break open the mitochondria and release the cristae. Tomography was completed using these crista membranes.
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Electron microscopy
Microscope
FEI TALOS ARCTICA
Electron beam
Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
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