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- EMDB-18644: Cryo-EM structure of Streptococcus pneumoniae NADPH oxidase -

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Basic information

Entry
Database: EMDB / ID: EMD-18644
TitleCryo-EM structure of Streptococcus pneumoniae NADPH oxidase
Map dataMain map used for atomic model building and figures.
Sample
  • Complex: Streptococcus pneumoniae NADPH oxidase with FAD and heme cofactors
    • Complex: Streptococcus pneumoniae NADPH oxidase
      • Protein or peptide: FAD-binding FR-type domain-containing protein
  • Ligand: FLAVIN-ADENINE DINUCLEOTIDE
  • Ligand: PROTOPORPHYRIN IX CONTAINING FE
  • Ligand: water
KeywordsNADPH oxidase / ROS producing / flavoprotein / heme protein / MEMBRANE PROTEIN
Function / homology
Function and homology information


2 iron, 2 sulfur cluster binding / flavin adenine dinucleotide binding / oxidoreductase activity / membrane
Similarity search - Function
: / FAD-binding 8 / FAD-binding domain / Oxidoreductase FAD/NAD(P)-binding / Oxidoreductase NAD-binding domain / FAD-binding domain, ferredoxin reductase-type / Ferredoxin-NADP reductase (FNR), nucleotide-binding domain / Ferredoxin reductase-type FAD binding domain profile. / Riboflavin synthase-like beta-barrel
Similarity search - Domain/homology
FAD-binding FR-type domain-containing protein
Similarity search - Component
Biological speciesStreptococcus pneumoniae (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.29 Å
AuthorsDubach VRA / San Segundo-Acosta P / Murphy BJ
Funding support Germany, 1 items
OrganizationGrant numberCountry
Max Planck Society Germany
CitationJournal: Nat Struct Mol Biol / Year: 2024
Title: Structural and mechanistic insights into Streptococcus pneumoniae NADPH oxidase.
Authors: Victor R A Dubach / Pablo San Segundo-Acosta / Bonnie J Murphy /
Abstract: Nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOXs) have a major role in the physiology of eukaryotic cells by mediating reactive oxygen species production. Evolutionarily distant ...Nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOXs) have a major role in the physiology of eukaryotic cells by mediating reactive oxygen species production. Evolutionarily distant proteins with the NOX catalytic core have been found in bacteria, including Streptococcus pneumoniae NOX (SpNOX), which is proposed as a model for studying NOXs because of its high activity and stability in detergent micelles. We present here cryo-electron microscopy structures of substrate-free and nicotinamide adenine dinucleotide (NADH)-bound SpNOX and of NADPH-bound wild-type and F397A SpNOX under turnover conditions. These high-resolution structures provide insights into the electron-transfer pathway and reveal a hydride-transfer mechanism regulated by the displacement of F397. We conducted structure-guided mutagenesis and biochemical analyses that explain the absence of substrate specificity toward NADPH and suggest the mechanism behind constitutive activity. Our study presents the structural basis underlying SpNOX enzymatic activity and sheds light on its potential in vivo function.
History
DepositionOct 12, 2023-
Header (metadata) releaseJul 24, 2024-
Map releaseJul 24, 2024-
UpdateJul 31, 2024-
Current statusJul 31, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

Downloads & links

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Map

FileDownload / File: emd_18644.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationMain map used for atomic model building and figures.
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1 Å/pix.
x 256 pix.
= 256.717 Å
1 Å/pix.
x 256 pix.
= 256.717 Å
1 Å/pix.
x 256 pix.
= 256.717 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.0028 Å
Density
Contour LevelBy AUTHOR: 0.727
Minimum - Maximum-3.621169 - 5.9997454
Average (Standard dev.)-0.0006244502 (±0.10096196)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 256.7168 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_18644_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Additional map: Raw unsharpened map obtained after merging of the half maps.

Fileemd_18644_additional_1.map
AnnotationRaw unsharpened map obtained after merging of the half maps.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Half map: Half map A used during refinement and FSC...

Fileemd_18644_half_map_1.map
AnnotationHalf map A used during refinement and FSC gold standard resolution estimation.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Half map: Half map B used during refinement and FSC...

Fileemd_18644_half_map_2.map
AnnotationHalf map B used during refinement and FSC gold standard resolution estimation.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Sample components

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Entire : Streptococcus pneumoniae NADPH oxidase with FAD and heme cofactors

EntireName: Streptococcus pneumoniae NADPH oxidase with FAD and heme cofactors
Components
  • Complex: Streptococcus pneumoniae NADPH oxidase with FAD and heme cofactors
    • Complex: Streptococcus pneumoniae NADPH oxidase
      • Protein or peptide: FAD-binding FR-type domain-containing protein
  • Ligand: FLAVIN-ADENINE DINUCLEOTIDE
  • Ligand: PROTOPORPHYRIN IX CONTAINING FE
  • Ligand: water

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Supramolecule #1: Streptococcus pneumoniae NADPH oxidase with FAD and heme cofactors

SupramoleculeName: Streptococcus pneumoniae NADPH oxidase with FAD and heme cofactors
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Streptococcus pneumoniae (bacteria)
Molecular weightTheoretical: 46 KDa

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Supramolecule #2: Streptococcus pneumoniae NADPH oxidase

SupramoleculeName: Streptococcus pneumoniae NADPH oxidase / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1
Source (natural)Organism: Streptococcus pneumoniae (bacteria)

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Macromolecule #1: FAD-binding FR-type domain-containing protein

MacromoleculeName: FAD-binding FR-type domain-containing protein / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Streptococcus pneumoniae (bacteria)
Molecular weightTheoretical: 46.060551 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: EFSMKSVKGL LFIIASFILT LLTWMNTSPQ FMIPGLALTS LSLTFILATR LPLLESWFHS LEKVYTVHKF TAFLSIILLI FHNFSMGGL WGSRLAAQFG NLAIYIFASI ILVAYLGKYI QYEAWRWIHR LVYLAYILGL FHIYMIMGNR LLTFNLLSFL V GSYALLGL ...String:
EFSMKSVKGL LFIIASFILT LLTWMNTSPQ FMIPGLALTS LSLTFILATR LPLLESWFHS LEKVYTVHKF TAFLSIILLI FHNFSMGGL WGSRLAAQFG NLAIYIFASI ILVAYLGKYI QYEAWRWIHR LVYLAYILGL FHIYMIMGNR LLTFNLLSFL V GSYALLGL LAGFYIIFLY QKISFPYLGK ITHLKRLNHD TREIQIHLSR PFNYQSGQFA FLKIFQEGFE SAPHPFSISG GH GQTLYFT VKTSGDHTKN IYDNLQAGSK VTLDRAYGHM IIEEGRENQV WIAGGIGITP FISYIREHPI LDKQVHFYYS FRG DENAVY LDLLRNYAQK NPNFELHLID STKDGYLNFE QKEVPEHATV YMCGPISMMK ALAKQIKKQN PKTELIYEGF KFK

UniProtKB: FAD-binding FR-type domain-containing protein

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Macromolecule #2: FLAVIN-ADENINE DINUCLEOTIDE

MacromoleculeName: FLAVIN-ADENINE DINUCLEOTIDE / type: ligand / ID: 2 / Number of copies: 1 / Formula: FAD
Molecular weightTheoretical: 785.55 Da
Chemical component information

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

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Macromolecule #3: PROTOPORPHYRIN IX CONTAINING FE

MacromoleculeName: PROTOPORPHYRIN IX CONTAINING FE / type: ligand / ID: 3 / Number of copies: 2 / Formula: HEM
Molecular weightTheoretical: 616.487 Da
Chemical component information

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

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Macromolecule #4: water

MacromoleculeName: water / type: ligand / ID: 4 / Number of copies: 63 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration6 mg/mL
BufferpH: 7.5
Component:
ConcentrationFormulaName
250.0 mMNaClsodium chloride
50.0 mMTris-HClTris-HCl
0.002 %LMNGLauryl Maltose Neopentyl Glycol
1.0 mMFADflavin-adenine dinucleotide
GridModel: UltrAuFoil R1.2/1.3 / Material: GOLD / Mesh: 400 / Support film - Material: GOLD / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 90 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK III

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Electron microscopy

MicroscopeTFS KRIOS
Specialist opticsEnergy filter - Name: TFS Selectris X / Energy filter - Slit width: 10 eV
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number grids imaged: 1 / Number real images: 18433 / Average exposure time: 3.96 sec. / Average electron dose: 70.0 e/Å2 / Details: Movies were collected in EER format.
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.1 µm / Nominal defocus min: 0.7000000000000001 µm / Nominal magnification: 215000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

DetailsMovies were collected in EER format and not gain corrected
Particle selectionNumber selected: 8765912
Details: These are the combined particles from two different Topaz models and a crYOLO model and contains duplicate particles.
Startup modelType of model: NONE
Final reconstructionAlgorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 2.29 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC
Details: Final refinement was local refinement in cryoSPARC 4.0 using a mask that excludes the detergent micelle.
Number images used: 397972
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC
Final 3D classificationSoftware - Name: cryoSPARC
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

Chain - Chain ID: A / Chain - Residue range: 1-400 / Chain - Source name: AlphaFold / Chain - Initial model type: in silico model
RefinementSpace: REAL / Protocol: RIGID BODY FIT / Target criteria: Cross-correlation coefficient
Output model

PDB-8qt6:
Cryo-EM structure of Streptococcus pneumoniae NADPH oxidase

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