+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-17763 | |||||||||
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タイトル | Structure of the human nuclear cap-binding complex bound to ARS2[147-871] and m7GTP | |||||||||
マップデータ | ||||||||||
試料 |
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キーワード | Nuclear cap-binding complex / ARS2 / Pol II transcript metabolism / RNA BINDING PROTEIN | |||||||||
機能・相同性 | 機能・相同性情報 mRNA cap binding complex binding / positive regulation of RNA binding / snRNA export from nucleus / nuclear cap binding complex / mRNA metabolic process / RNA cap binding complex / histone mRNA metabolic process / positive regulation of mRNA 3'-end processing / positive regulation of RNA export from nucleus / cap-dependent translational initiation ...mRNA cap binding complex binding / positive regulation of RNA binding / snRNA export from nucleus / nuclear cap binding complex / mRNA metabolic process / RNA cap binding complex / histone mRNA metabolic process / positive regulation of mRNA 3'-end processing / positive regulation of RNA export from nucleus / cap-dependent translational initiation / Processing of Intronless Pre-mRNAs / snRNA binding / RNA cap binding / alternative mRNA splicing, via spliceosome / neuronal stem cell population maintenance / miRNA-mediated post-transcriptional gene silencing / primary miRNA processing / regulation of mRNA processing / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / regulatory ncRNA-mediated post-transcriptional gene silencing / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA / RNA 7-methylguanosine cap binding / response to arsenic-containing substance / Transport of Mature mRNA Derived from an Intronless Transcript / mRNA 3'-end processing / mRNA 3'-end processing / RNA catabolic process / mRNA cis splicing, via spliceosome / Transport of Mature mRNA derived from an Intron-Containing Transcript / Abortive elongation of HIV-1 transcript in the absence of Tat / positive regulation of mRNA splicing, via spliceosome / regulation of translational initiation / FGFR2 alternative splicing / RNA Polymerase II Transcription Termination / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / positive regulation of neurogenesis / Signaling by FGFR2 IIIa TM / Formation of the Early Elongation Complex / Formation of the HIV-1 Early Elongation Complex / mRNA Capping / spliceosomal complex assembly / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / RNA polymerase II transcribes snRNA genes / 7-methylguanosine mRNA capping / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Formation of HIV-1 elongation complex containing HIV-1 Tat / Formation of HIV elongation complex in the absence of HIV Tat / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / mRNA export from nucleus / Formation of RNA Pol II elongation complex / RNA Polymerase II Pre-transcription Events / mRNA Splicing - Major Pathway / RNA splicing / positive regulation of transcription elongation by RNA polymerase II / mRNA transcription by RNA polymerase II / Regulation of expression of SLITs and ROBOs / mRNA splicing, via spliceosome / mRNA processing / protein-macromolecule adaptor activity / snRNP Assembly / positive regulation of cell growth / defense response to virus / molecular adaptor activity / nuclear body / ribonucleoprotein complex / mRNA binding / regulation of DNA-templated transcription / protein-containing complex / mitochondrion / DNA binding / RNA binding / nucleoplasm / nucleus / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.43 Å | |||||||||
データ登録者 | Dubiez E / Pellegrini E / Foucher AE / Cusack S / Kadlec J | |||||||||
資金援助 | フランス, 1件
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引用 | ジャーナル: Cell Rep / 年: 2024 タイトル: Structural basis for competitive binding of productive and degradative co-transcriptional effectors to the nuclear cap-binding complex. 著者: Etienne Dubiez / Erika Pellegrini / Maja Finderup Brask / William Garland / Anne-Emmanuelle Foucher / Karine Huard / Torben Heick Jensen / Stephen Cusack / Jan Kadlec / 要旨: The nuclear cap-binding complex (CBC) coordinates co-transcriptional maturation, transport, or degradation of nascent RNA polymerase II (Pol II) transcripts. CBC with its partner ARS2 forms mutually ...The nuclear cap-binding complex (CBC) coordinates co-transcriptional maturation, transport, or degradation of nascent RNA polymerase II (Pol II) transcripts. CBC with its partner ARS2 forms mutually exclusive complexes with diverse "effectors" that promote either productive or destructive outcomes. Combining AlphaFold predictions with structural and biochemical validation, we show how effectors NCBP3, NELF-E, ARS2, PHAX, and ZC3H18 form competing binary complexes with CBC and how PHAX, NCBP3, ZC3H18, and other effectors compete for binding to ARS2. In ternary CBC-ARS2 complexes with PHAX, NCBP3, or ZC3H18, ARS2 is responsible for the initial effector recruitment but inhibits their direct binding to the CBC. We show that in vivo ZC3H18 binding to both CBC and ARS2 is required for nuclear RNA degradation. We propose that recruitment of PHAX to CBC-ARS2 can lead, with appropriate cues, to competitive displacement of ARS2 and ZC3H18 from the CBC, thus promoting a productive rather than a degradative RNA fate. | |||||||||
履歴 |
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-構造の表示
添付画像 |
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-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_17763.map.gz | 89.1 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-17763-v30.xml emd-17763.xml | 17.8 KB 17.8 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_17763_fsc.xml | 11.9 KB | 表示 | FSCデータファイル |
画像 | emd_17763.png | 34 KB | ||
マスクデータ | emd_17763_msk_1.map | 178 MB | マスクマップ | |
Filedesc metadata | emd-17763.cif.gz | 6.6 KB | ||
その他 | emd_17763_half_map_1.map.gz emd_17763_half_map_2.map.gz | 165.4 MB 165.4 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-17763 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-17763 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_17763.map.gz / 形式: CCP4 / 大きさ: 178 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 0.839 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-マスク #1
ファイル | emd_17763_msk_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_17763_half_map_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #2
ファイル | emd_17763_half_map_2.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
-全体 : Structure of the human nuclear cap-binding complex bound to ARS2[...
全体 | 名称: Structure of the human nuclear cap-binding complex bound to ARS2[147-871] and m7GTP |
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要素 |
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-超分子 #1: Structure of the human nuclear cap-binding complex bound to ARS2[...
超分子 | 名称: Structure of the human nuclear cap-binding complex bound to ARS2[147-871] and m7GTP タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#3 |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
-分子 #1: Nuclear cap-binding protein subunit 1
分子 | 名称: Nuclear cap-binding protein subunit 1 / タイプ: protein_or_peptide / ID: 1 詳細: CBP80 construct has the N-terminal 20 residues (containing the NLS) deleted and replaced by a methionine コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 89.781938 KDa |
組換発現 | 生物種: Trichoplusia ni (イラクサキンウワバ) |
配列 | 文字列: MKTSDANETE DHLESLICKV GEKSACSLES NLEGLAGVLE ADLPNYKSKI LRLLCTVARL LPEKLTIYTT LVGLLNARNY NFGGEFVEA MIRQLKESLK ANNYNEAVYL VRFLSDLVNC HVIAAPSMVA MFENFVSVTQ EEDVPQVRRD WYVYAFLSSL P WVGKELYE ...文字列: MKTSDANETE DHLESLICKV GEKSACSLES NLEGLAGVLE ADLPNYKSKI LRLLCTVARL LPEKLTIYTT LVGLLNARNY NFGGEFVEA MIRQLKESLK ANNYNEAVYL VRFLSDLVNC HVIAAPSMVA MFENFVSVTQ EEDVPQVRRD WYVYAFLSSL P WVGKELYE KKDAEMDRIF ANTESYLKRR QKTHVPMLQV WTADKPHPQE EYLDCLWAQI QKLKKDRWQE RHILRPYLAF DS ILCEALQ HNLPPFTPPP HTEDSVYPMP RVIFRMFDYT DDPEGPVMPG SHSVERFVIE ENLHCIIKSH WKERKTCAAQ LVS YPGKNK IPLNYHIVEV IFAELFQLPA PPHIDVMYTT LLIELCKLQP GSLPQVLAQA TEMLYMRLDT MNTTCVDRFI NWFS HHLSN FQFRWSWEDW SDCLSQDPES PKPKFVREVL EKCMRLSYHQ RILDIVPPTF SALCPANPTC IYKYGDESSN SLPGH SVAL CLAVAFKSKA TNDEIFSILK DVPNPNQDDD DDEGFSFNPL KIEVFVQTLL HLAAKSFSHS FSALAKFHEV FKTLAE SDE GKLHVLRVMF EVWRNHPQMI AVLVDKMIRT QIVDCAAVAN WIFSSELSRD FTRLFVWEIL HSTIRKMNKH VLKIQKE LE EAKEKLARQH KRRSDDDDRS SDRKDGVLEE QIERLQEKVE SAQSEQKNLF LVIFQRFIMI LTEHLVRCET DGTSVLTP W YKNCIERLQQ IFLQHHQIIQ QYMVTLENLL FTAELDPHIL AVFQQFCALQ A UniProtKB: Nuclear cap-binding protein subunit 1 |
-分子 #2: Nuclear cap-binding protein subunit 2
分子 | 名称: Nuclear cap-binding protein subunit 2 / タイプ: protein_or_peptide / ID: 2 / 詳細: Additional GA at the N-terminus / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 18.028131 KDa |
組換発現 | 生物種: Escherichia coli (大腸菌) |
配列 | 文字列: MSGGLLKALR SDSYVELSQY RDQHFRGDNE EQEKLLKKSC TLYVGNLSFY TTEEQIYELF SKSGDIKKII MGLDKMKKTA CGFCFVEYY SRADAENAMR YINGTRLDDR IIRTDWDAGF KEGRQYGRGR SGGQVRDEYR QDYDAGRGGY GKLAQNQ UniProtKB: Nuclear cap-binding protein subunit 2 |
-分子 #3: Serrate RNA effector molecule homolog
分子 | 名称: Serrate RNA effector molecule homolog / タイプ: protein_or_peptide / ID: 3 詳細: ARS2 has N-terminal 147 residues deleted and replaced by a methionine コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 83.061391 KDa |
組換発現 | 生物種: Trichoplusia ni (イラクサキンウワバ) |
配列 | 文字列: VMKTFKEFLL SLDDSVDETE AVKRYNDYKL DFRRQQMQDF FLAHKDEEWF RSKYHPDEVG KRRQEARGAL QNRLRVFLSL METGWFDNL LLDIDKADAI VKMLDAAVIK MEGGTENDLR ILEQEEEEEQ AGKPGEPSKK EEGRAGAGLG DGERKTNDKD E KKEDGKQA ...文字列: VMKTFKEFLL SLDDSVDETE AVKRYNDYKL DFRRQQMQDF FLAHKDEEWF RSKYHPDEVG KRRQEARGAL QNRLRVFLSL METGWFDNL LLDIDKADAI VKMLDAAVIK MEGGTENDLR ILEQEEEEEQ AGKPGEPSKK EEGRAGAGLG DGERKTNDKD E KKEDGKQA ENDSSNDDKT KKSEGDGDKE EKKEDSEKEA KKSSKKRNRK HSGDDSFDEG SVSESESESE SGQAEEEKEE AE EALKEKE KPKEEEWEKP KDAAGLECKP RPLHKTCSLF MRNIAPNISR AEIISLCKRY PGFMRVALSE PQPERRFFRR GWV TFDRSV NIKEICWNLQ NIRLRECELS PGVNRDLTRR VRNINGITQH KQIVRNDIKL AAKLIHTLDD RTQLWASEPG TPPL PTSLP SQNPILKNIT DYLIEEVSAE EEELLGSSGG APPEEPPKEG NPAEINVERD EKLIKVLDKL LLYLRIVHSL DYYNT CEYP NEDEMPNRCG IIHVRGPMPP NRISHGEVLE WQKTFEEKLT PLLSVRESLS EEEAQKMGRK DPEQEVEKFV TSNTQE LGK DKWLCPLSGK KFKGPEFVRK HIFNKHAEKI EEVKKEVAFF NNFLTDAKRP ALPEIKPAQP PGPAQILPPG LTPGLPY PH QTPQGLMPYG QPRPPILGYG AGAVRPAVPT GGPPYPHAPY GAGRGNYDAF RGQGGYPGKP RNRMVRGDPR AIVEYRDL D APDDVDFF UniProtKB: Serrate RNA effector molecule homolog |
-分子 #4: 7N-METHYL-8-HYDROGUANOSINE-5'-TRIPHOSPHATE
分子 | 名称: 7N-METHYL-8-HYDROGUANOSINE-5'-TRIPHOSPHATE / タイプ: ligand / ID: 4 / コピー数: 1 / 式: MGT |
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分子量 | 理論値: 539.223 Da |
Chemical component information | ChemComp-MGT: |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 8 |
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凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 平均電子線量: 40.13 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.5 µm 最小 デフォーカス(公称値): 0.7000000000000001 µm |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |