EMDB-17719: human mitoribosomal large subunit assembly intermediate 1 with GT...

Basic information

Entry

Database: EMDB / ID: EMD-17719

• Title: human mitoribosomal large subunit assembly intermediate 1 with GTPBP10-GTPBP7
• Map data: full map, sharpened

Sample

• Complex: large mitoribosomal subunit biogenesis intermediate with GTPBP10, GTPBP7, NSUN4-MTERF4, ACP-module
  • Protein or peptide: x 55 types
  • RNA: x 1 types
• Protein or peptide: x 1 types
• RNA: x 1 types
• Ligand: x 6 types

• Keywords: mitochondria / maturation / biogenesis / ribosome / GTPBP7 / GTPBP10 / GTP Binding Protein 10 / OBGH2 / MTG1 / LSU

Function / homology

Function:

regulation of respiratory system process / rRNA modification in the mitochondrion / regulation of mitochondrial translation / negative regulation of mitochondrial translation / mitochondrial large ribosomal subunit assembly / Complex I biogenesis / negative regulation of ribosome biogenesis / rRNA (cytosine-C5-)-methyltransferase activity / tRNA (cytidine-5-)-methyltransferase activity / protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation / rRNA import into mitochondrion / rRNA methyltransferase activity / Respiratory electron transport / mitochondrial transcription / mitochondrial translational elongation / mitochondrial translational termination / mitochondrial ribosome assembly / translation release factor activity, codon nonspecific / microprocessor complex / iron-sulfur cluster assembly complex / Mitochondrial translation elongation / Mitochondrial translation termination / Mitochondrial translation initiation / positive regulation of mitochondrial translation / mitochondrial large ribosomal subunit binding / protein targeting to mitochondrion / Glyoxylate metabolism and glycine degradation / mitochondrial fission / camera-type eye development / Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters / mitochondrial large ribosomal subunit / peptidyl-tRNA hydrolase / [2Fe-2S] cluster assembly / mitochondrial small ribosomal subunit / rRNA methylation / aminoacyl-tRNA hydrolase activity / iron-sulfur cluster assembly / mitochondrial ribosome / mitochondrial translation / ribosomal large subunit binding / : / mitochondrial electron transport, NADH to ubiquinone / proton motive force-driven mitochondrial ATP synthesis / mitochondrial respiratory chain complex I assembly / acyl binding / anatomical structure morphogenesis / acyl carrier activity / RNA processing / Mitochondrial protein degradation / aerobic respiration / rescue of stalled ribosome / Transferases; Transferring one-carbon groups; Methyltransferases / ribosomal large subunit biogenesis / cellular response to leukemia inhibitory factor / methyltransferase activity / fatty acid binding / mitochondrial membrane / fibrillar center / fatty acid biosynthetic process / rRNA processing / double-stranded RNA binding / ribosome biogenesis / cell junction / chromosome / heart development / small ribosomal subunit rRNA binding / 5S rRNA binding / large ribosomal subunit rRNA binding / double-stranded DNA binding / endonuclease activity / cytosolic large ribosomal subunit / mitochondrial inner membrane / cytoplasmic translation / nuclear body / rRNA binding / negative regulation of translation / ribosome / structural constituent of ribosome / mitochondrial matrix / ribonucleoprotein complex / translation / cell cycle / protein domain specific binding / nucleotide binding / GTPase activity / mRNA binding / apoptotic process / synapse / calcium ion binding / regulation of DNA-templated transcription / nucleolus / GTP binding / magnesium ion binding / mitochondrion / RNA binding / extracellular space / nucleoplasm / nucleus / plasma membrane

Domain/homology:

GTPase, MTG1 / GTP-binding protein, ribosome biogenesis / OBG-type GTPase / GTP1/OBG domain / GTP-binding protein Obg/CgtA / GTP1/OBG domain superfamily / GTP1/OBG / Obg domain profile. / Mitochondrial termination factor repeats / Transcription termination factor, mitochondrial/chloroplastic / MTERF superfamily, mitochondrial/chloroplastic / mTERF / MIEF1-MP, LYR domain / Ribosomal silencing factor during starvation / Protein Iojap/ribosomal silencing factor RsfS / : / SAM-dependent methyltransferase RsmB/NOP2-type / RNA (C5-cytosine) methyltransferase / 16S rRNA methyltransferase RsmB/F / SAM-dependent MTase RsmB/NOP-type domain profile. / Ribosomal protein L37, mitochondrial / Ribosomal protein L55, mitochondrial / Mitochondrial ribosomal protein L48 / 39S ribosomal protein L40, mitochondrial / Mitochondrial ribosomal protein L55 superfamily / Mitochondrial ribosomal protein L37 / Mitochondrial ribosomal protein L55 / Mitochondrial ribosomal protein L46 NUDIX / Ribosomal protein S30, mitochondrial / Ribosomal protein L53, mitochondrial / 39S ribosomal protein L53/MRP-L53 / 39S ribosomal protein L42, mitochondrial / Mitochondrial 28S ribosomal protein S32 / Ribosomal protein L28/L40, mitochondrial / Mitochondrial ribosomal protein L28 / Ribosomal protein 63, mitochondrial / Growth arrest/ DNA-damage-inducible protein-interacting protein 1 / Ribosomal protein L51, mitochondrial / Growth arrest and DNA-damage-inducible proteins-interacting protein 1 / Mitochondrial ribosomal subunit / Mitochondrial ribosome protein 63 / Ribosomal protein L37/S30 / Growth arrest and DNA damage-inducible proteins-interacting protein 1 domain superfamily / Mitochondrial 28S ribosomal protein S30 (PDCD9) / 39S ribosomal protein L52, mitochondrial / Mitoribosomal protein mL52 / Ribosomal protein L35, mitochondrial / MRPL44, double-stranded RNA binding domain / Tim44-like domain / Tim44-like domain / Tim44 / GTP-binding protein, orthogonal bundle domain superfamily / Ribosomal protein L49/IMG2 / Mitochondrial large subunit ribosomal protein (Img2) / Ribosomal protein L46, N-terminal / 39S mitochondrial ribosomal protein L46 / Ribosomal protein L50, mitochondria / Ribosomal protein L27/L41, mitochondrial / Mitochondrial ribosomal protein L27 / Ribosomal subunit 39S / 39S ribosomal protein L46, mitochondrial / 39S ribosomal protein L43/54S ribosomal protein L51 / Ribosomal protein L47, mitochondrial / MRP-L47 superfamily, mitochondrial / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein, eukaryotic / PEBP-like superfamily / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain profile. / Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily / TGS domain profile. / TGS / TGS-like / OBG-type guanine nucleotide-binding (G) domain / OBG-type guanine nucleotide-binding (G) domain profile. / Peptide chain release factor class I / RF-1 domain / Ribonuclease III, endonuclease domain superfamily / 50S ribosome-binding GTPase / GTP binding domain / Double stranded RNA-binding domain (dsRBD) profile. / Double-stranded RNA-binding domain / Complex 1 LYR protein domain / Complex 1 protein (LYR family) / : / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Acyl carrier protein (ACP) / Beta-grasp domain superfamily / NTF2-like domain superfamily / NUDIX hydrolase-like domain superfamily / Ribosomal protein L11, bacterial-type / Phosphopantetheine attachment site / Phosphopantetheine attachment site. / Nucleotidyltransferase superfamily / Ribosomal protein L10-like domain superfamily / Ribosomal protein L10

Component:

GTP-binding protein 10 / Mitochondrial ribosome and complex I assembly factor AltMIEF1 / Acyl carrier protein, mitochondrial / Large ribosomal subunit protein bL33m / Large ribosomal subunit protein uL3m / Large ribosomal subunit protein bL19m / Large ribosomal subunit protein bL28m / Large ribosomal subunit protein mL49 / Large ribosomal subunit protein mL62 / Large ribosomal subunit protein uL23m / Large ribosomal subunit protein mL51 / Large ribosomal subunit protein uL2m / Large ribosomal subunit protein mL54 / Large ribosomal subunit protein uL14m / Large ribosomal subunit protein bL21m / Transcription termination factor 4, mitochondrial / Large ribosomal subunit protein mL55 / Large ribosomal subunit protein uL10m / Large ribosomal subunit protein mL52 / Large ribosomal subunit protein mL41 / Large ribosomal subunit protein mL50 / Large ribosomal subunit protein mL43 / Large ribosomal subunit protein mL64 / Large ribosomal subunit protein uL30m / Large ribosomal subunit protein uL24m / 5-methylcytosine rRNA methyltransferase NSUN4 / Large ribosomal subunit protein mL38 / Mitochondrial assembly of ribosomal large subunit protein 1 / Large ribosomal subunit protein mL53 / Large ribosomal subunit protein mL48 / Large ribosomal subunit protein bL34m / Large ribosomal subunit protein mL63 / Large ribosomal subunit protein mL45 / Mitochondrial ribosome-associated GTPase 1 / Large ribosomal subunit protein bL32m / Large ribosomal subunit protein bL20m / Large ribosomal subunit protein uL13m / Large ribosomal subunit protein bL9m / Large ribosomal subunit protein uL4m / Large ribosomal subunit protein mL37 / Large ribosomal subunit protein uL18m / Large ribosomal subunit protein mL46 / Large ribosomal subunit protein mL44 / Large ribosomal subunit protein uL29m / Large ribosomal subunit protein mL65 / Large ribosomal subunit protein mL40 / Large ribosomal subunit protein bL17m / Large ribosomal subunit protein mL66 / Large ribosomal subunit protein uL22m / Large ribosomal subunit protein uL16m / Large ribosomal subunit protein mL39 / Large ribosomal subunit protein bL35m / Large ribosomal subunit protein uL15m / Large ribosomal subunit protein bL27m / Large ribosomal subunit protein uL11m / Large ribosomal subunit protein mL42

• Biological species: Homo sapiens (human)
• Method: single particle reconstruction / cryo EM / Resolution: 3.03 Å
• Authors: Kummer E / Nguyen TG / Ritter C

Funding support

Denmark, 1 items

Organization	Grant number	Country
Novo Nordisk Foundation	NNF21OC0067360	Denmark

• Citation: Journal: Nat Commun / Year: 2023; Title: Structural insights into the role of GTPBP10 in the RNA maturation of the mitoribosome.; Authors: Thu Giang Nguyen / Christina Ritter / Eva Kummer / ; Abstract: Mitochondria contain their own genetic information and a dedicated translation system to express it. The mitochondrial ribosome is assembled from mitochondrial-encoded RNA and nuclear-encoded ribosomal proteins. Assembly is coordinated in the mitochondrial matrix by biogenesis factors that transiently associate with the maturing particle. Here, we present a structural snapshot of a large mitoribosomal subunit assembly intermediate containing 7 biogenesis factors including the GTPases GTPBP7 and GTPBP10. Our structure illustrates how GTPBP10 aids the folding of the ribosomal RNA during the biogenesis process, how this process is related to bacterial ribosome biogenesis, and why mitochondria require two biogenesis factors in contrast to only one in bacteria.

History

Deposition	Jun 23, 2023	-
Header (metadata) release	Dec 13, 2023	-
Map release	Dec 13, 2023	-
Update	Jun 26, 2024	-
Current status	Jun 26, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_17719.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: full map, sharpened
• Voxel size: X=Y=Z: 1.08 Å

Density

Contour Level	By AUTHOR: 0.747
Minimum - Maximum	-2.1063588 - 4.0282693
Average (Standard dev.)	-0.0036343343 (±0.15991145)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 480 480 480 Spacing 480 480 480
Cell	A=B=C: 518.4 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_17719_msk_1.map

Additional map: full map, unsharpened

• File: emd_17719_additional_1.map
• Annotation: full map, unsharpened

Half map: #2

• File: emd_17719_half_map_1.map

Half map: #1

• File: emd_17719_half_map_2.map

Sample components

Entire : large mitoribosomal subunit biogenesis intermediate with GTPBP10,...

• Entire: Name: large mitoribosomal subunit biogenesis intermediate with GTPBP10, GTPBP7, NSUN4-MTERF4, ACP-module

Components

• Complex: large mitoribosomal subunit biogenesis intermediate with GTPBP10, GTPBP7, NSUN4-MTERF4, ACP-module
  • Protein or peptide: 39S ribosomal protein L32, mitochondrial
  • Protein or peptide: 39S ribosomal protein L33, mitochondrial
  • Protein or peptide: 39S ribosomal protein L34, mitochondrial
  • Protein or peptide: 39S ribosomal protein L35, mitochondrial
  • Protein or peptide: 39S ribosomal protein L37, mitochondrial
  • Protein or peptide: 39S ribosomal protein L38, mitochondrial
  • Protein or peptide: 39S ribosomal protein L39, mitochondrial
  • Protein or peptide: 39S ribosomal protein L40, mitochondrial
  • Protein or peptide: 39S ribosomal protein L41, mitochondrial
  • RNA: CP tRNA-Val
  • Protein or peptide: 39S ribosomal protein L2, mitochondrial
  • Protein or peptide: 39S ribosomal protein L3, mitochondrial
  • Protein or peptide: 39S ribosomal protein L4, mitochondrial
  • Protein or peptide: 39S ribosomal protein L9, mitochondrial
  • Protein or peptide: 39S ribosomal protein L10, mitochondrial
  • Protein or peptide: 39S ribosomal protein L11, mitochondrial
  • Protein or peptide: 39S ribosomal protein L13, mitochondrial
  • Protein or peptide: 39S ribosomal protein L14, mitochondrial
  • Protein or peptide: 39S ribosomal protein L15, mitochondrial
  • Protein or peptide: 39S ribosomal protein L16, mitochondrial
  • Protein or peptide: 39S ribosomal protein L17, mitochondrial
  • Protein or peptide: 39S ribosomal protein L18, mitochondrial
  • Protein or peptide: 39S ribosomal protein L19, mitochondrial
  • Protein or peptide: 39S ribosomal protein L20, mitochondrial
  • Protein or peptide: 39S ribosomal protein L21, mitochondrial
  • Protein or peptide: 39S ribosomal protein L22, mitochondrial
  • Protein or peptide: 39S ribosomal protein L23, mitochondrial
  • Protein or peptide: 39S ribosomal protein L24, mitochondrial
  • Protein or peptide: 39S ribosomal protein L27, mitochondrial
  • Protein or peptide: 39S ribosomal protein L28, mitochondrial
  • Protein or peptide: 39S ribosomal protein L47, mitochondrial
  • Protein or peptide: 39S ribosomal protein L30, mitochondrial
  • Protein or peptide: 39S ribosomal protein L42, mitochondrial
  • Protein or peptide: 39S ribosomal protein L44, mitochondrial
  • Protein or peptide: 39S ribosomal protein L45, mitochondrial
  • Protein or peptide: 39S ribosomal protein L46, mitochondrial
  • Protein or peptide: 39S ribosomal protein L48, mitochondrial
  • Protein or peptide: 39S ribosomal protein L49, mitochondrial
  • Protein or peptide: 39S ribosomal protein L50, mitochondrial
  • Protein or peptide: 39S ribosomal protein L51, mitochondrial
  • Protein or peptide: 39S ribosomal protein L52, mitochondrial
  • Protein or peptide: 39S ribosomal protein L53, mitochondrial
  • Protein or peptide: 39S ribosomal protein L54, mitochondrial
  • Protein or peptide: 39S ribosomal protein L55, mitochondrial
  • Protein or peptide: Ribosomal protein 63, mitochondrial
  • Protein or peptide: Peptidyl-tRNA hydrolase ICT1, mitochondrial
  • Protein or peptide: Growth arrest and DNA damage-inducible proteins-interacting protein 1
  • Protein or peptide: 39S ribosomal protein S18a, mitochondrial
  • Protein or peptide: 39S ribosomal protein S30, mitochondrial
  • Protein or peptide: GTP-binding protein 10
  • Protein or peptide: Mitochondrial assembly of ribosomal large subunit protein 1
  • Protein or peptide: MIEF1 upstream open reading frame protein
  • Protein or peptide: Acyl carrier protein, mitochondrial
  • Protein or peptide: 5-methylcytosine rRNA methyltransferase NSUN4
  • Protein or peptide: Transcription termination factor 4, mitochondrial
  • Protein or peptide: Mitochondrial ribosome-associated GTPase 1
• Protein or peptide: Large ribosomal subunit protein mL43
• RNA: 16S rRNA + pre-H68-71 segment
• Ligand: ZINC ION
• Ligand: S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE
• Ligand: S-ADENOSYLMETHIONINE
• Ligand: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
• Ligand: MAGNESIUM ION
• Ligand: POTASSIUM ION

Supramolecule #1: large mitoribosomal subunit biogenesis intermediate with GTPBP10,...

• Supramolecule: Name: large mitoribosomal subunit biogenesis intermediate with GTPBP10, GTPBP7, NSUN4-MTERF4, ACP-module; type: complex / ID: 1 / Parent: 0 ; Macromolecule list: #1, #4, #7, #10, #13, #16, #19, #21, #23, #27, #29, #31, #33, #35, #37, #39, #41, #43, #45, #47, #49, #51, #53, #55, #57, #2, #5, #8, #11, #14, #17, #20, #22, #26, #28, #30, #32, #34, #36, #38, #40, #42, #44, #46, #48, #50, #52, #54, #56, #58, #3, #6, #9, #12, #15, #18
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 1.9 MDa

Macromolecule #1: 39S ribosomal protein L32, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L32, mitochondrial / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.437799 KDa

Sequence

String:

MALAMLVLVV SPWSAARGVL RNYWERLLRK LPQSRPGFPS PPWGPALAVQ GPAMFTEPAN DTSGSKENSS LLDSIFWMAA PKNRRTIEV NRCRRRNPQK LIKVKNNIDV CPECGHLKQK HVLCAYCYEK VCKETAEIRR QIGKQEGGPF KAPTIETVVL Y TGETPSEQ DQGKRIIERD RKRPSWFTQN

UniProtKB: Large ribosomal subunit protein bL32m

Macromolecule #2: 39S ribosomal protein L22, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L22, mitochondrial / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.683732 KDa

Sequence

String:

MAAAVLGQLG ALWIHNLRSR GKLALGVLPQ SYIHTSASLD ISRKWEKKNK IVYPPQLPGE PRRPAEIYHC RRQIKYSKDK MWYLAKLIR GMSIDQALAQ LEFNDKKGAK IIKEVLLEAQ DMAVRDHNVE FRSNLYIAES TSGRGQCLKR IRYHGRGRFG I MEKVYCHY FVKLVEGPPP PPEPPKTAVA HAKEYIQQLR SRTIVHTL

UniProtKB: Large ribosomal subunit protein uL22m

Macromolecule #3: Mitochondrial assembly of ribosomal large subunit protein 1

• Macromolecule: Name: Mitochondrial assembly of ribosomal large subunit protein 1; type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.203076 KDa

Sequence

String:

MGPGGRVARL LAPLMWRRAV SSVAGSAVGA EPGLRLLAVQ RLPVGAAFCR ACQTPNFVRG LHSEPGLEER AEGTVNEGRP ESDAADHTG PKFDIDMMVS LLRQENARDI CVIQVPPEMR YTDYFVIVSG TSTRHLHAMA FYVVKMYKHL KCKRDPHVKI E GKDTDDWL CVDFGSMVIH LMLPETREIY ELEKLWTLRS YDDQLAQIAP ETVPEDFILG IEDDTSSVTP VELKCE

UniProtKB: Mitochondrial assembly of ribosomal large subunit protein 1

Macromolecule #4: 39S ribosomal protein L33, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L33, mitochondrial / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 7.635204 KDa

Sequence

String:

MFLSAVFFAK SKSKNILVRM VSEAGTGFCF NTKRNRLREK LTLLHYDPVV KQRVLFVEKK KIRSL

UniProtKB: Large ribosomal subunit protein bL33m

Macromolecule #5: 39S ribosomal protein L23, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L23, mitochondrial / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.849227 KDa

Sequence

String:

M(AYA)RNVVYPLY RLGGPQLRVF RTNFFIQLVR PGVAQPEDTV QFRIPMEMTR VDLRNYLEGI YNVPVAAVRT RVQHGS NKR RDHRNVRIKK PDYKVAYVQL AHGQTFTFPD LFPEKDESPE GSAADDLYSM LEEERQQRQS SDPRRGGVPS WFGL

UniProtKB: Large ribosomal subunit protein uL23m

Macromolecule #6: MIEF1 upstream open reading frame protein

• Macromolecule: Name: MIEF1 upstream open reading frame protein / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 8.460787 KDa

Sequence

String:

MAPWSREAVL SLYRALLRQG RQLRYTDRDF YFASIRREFR KNQKLEDAEA RERQLEKGLV FLNGKLGRII

UniProtKB: Mitochondrial ribosome and complex I assembly factor AltMIEF1

Macromolecule #7: 39S ribosomal protein L34, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L34, mitochondrial / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 10.183913 KDa

Sequence

String:

MAVLAGSLLG PTSRSAALLG GRWLQPRAWL GFPDAWGLPT PQQARGKARG NEYQPSNIKR KNKHGWVRRL STPAGVQVIL RRMLKGRKS LSH

UniProtKB: Large ribosomal subunit protein bL34m

Macromolecule #8: 39S ribosomal protein L24, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L24, mitochondrial / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.953875 KDa

Sequence

String:

MRLSALLALA SKVTLPPHYR YGMSPPGSVA DKRKNPPWIR RRPVVVEPIS DEDWYLFCGD TVEILEGKDA GKQGKVVQVI RQRNWVVVG GLNTHYRYIG KTMDYRGTMI PSEAPLLHRQ VKLVDPMDRK PTEIEWRFTE AGERVRVSTR SGRIIPKPEF P RADGIVPE TWIDGPKDTS VEDALERTYV PCLKTLQEEV MEAMGIKETR KYKKVYWY

UniProtKB: Large ribosomal subunit protein uL24m

Macromolecule #9: Acyl carrier protein, mitochondrial

• Macromolecule: Name: Acyl carrier protein, mitochondrial / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.434273 KDa

Sequence

String:

MASRVLSAYV SRLPAAFAPL PRVRMLAVAR PLSTALCSAG TQTRLGTLQP ALVLAQVPGR VTQLCRQYSD MPPLTLEGIQ DRVLYVLKL YDKIDPEKLS VNSHFMKDLG LDSLDQVEII MAMEDEFGFE IPDIDAEKLM CPQEIVDYIA DKKDVYE

UniProtKB: Acyl carrier protein, mitochondrial

Macromolecule #10: 39S ribosomal protein L35, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L35, mitochondrial / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.558488 KDa

Sequence

String:

MAASAFAGAV RAASGILRPL NILASSTYRN CVKNASLISA LSTGRFSHIQ TPVVSSTPRL TTSERNLTCG HTSVILNRMA PVLPSVLKL PVRSLTYFSA RKGKRKTVKA VIDRFLRLHC GLWVRRKAGY KKKLWKKTPA RKKRLREFVF CNKTQSKLLD K MTTSFWKR RNWYVDDPYQ KYHDRTNLKV

UniProtKB: Large ribosomal subunit protein bL35m

Macromolecule #11: 39S ribosomal protein L27, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L27, mitochondrial / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.102859 KDa

Sequence

String:

MASVVLALRT RTAVTSLLSP TPATALAVRY ASKKSGGSSK NLGGKSSGRR QGIKKMEGHY VHAGNIIATQ RHFRWHPGAH VGVGKNKCL YALEEGIVRY TKEVYVPHPR NTEAVDLITR LPKGAVLYKT FVHVVPAKPE GTFKLVAML

UniProtKB: Large ribosomal subunit protein bL27m

Macromolecule #12: 5-methylcytosine rRNA methyltransferase NSUN4

• Macromolecule: Name: 5-methylcytosine rRNA methyltransferase NSUN4 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO; EC number: Transferases; Transferring one-carbon groups; Methyltransferases
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 43.140473 KDa

Sequence

String:

MAALTLRGVR ELLKRVDLAT VPRRHRYKKK WAATEPKFPA VRLALQNFDM TYSVQFGDLW PSIRVSLLSE QKYGALVNNF AAWDHVSAK LEQLSAKDFV NEAISHWELQ SEGGQSAAPS PASWACSPNL RCFTFDRGDI SRFPPARPGS LGVMEYYLMD A ASLLPVLA LGLQPGDIVL DLCAAPGGKT LALLQTGCCR NLAANDLSPS RIARLQKILH SYVPEEIRDG NQVRVTSWDG RK WGELEGD TYDRVLVDVP CTTDRHSLHE EENNIFKRSR KKERQILPVL QVQLLAAGLL ATKPGGHVVY STCSLSHLQN EYV VQGAIE LLANQYSIQV QVEDLTHFRR VFMDTFCFFS SCQVGELVIP NLMANFGPMY FCKMRRLT

UniProtKB: 5-methylcytosine rRNA methyltransferase NSUN4

Macromolecule #13: 39S ribosomal protein L37, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L37, mitochondrial / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 48.183344 KDa

Sequence

String:

MALASGPARR ALAGSGQLGL GGFGAPRRGA YEWGVRSTRK SEPPPLDRVY EIPGLEPITF AGKMHFVPWL ARPIFPPWDR GYKDPRFYR SPPLHEHPLY KDQACYIFHH RCRLLEGVKQ ALWLTKTKLI EGLPEKVLSL VDDPRNHIEN QDECVLNVIS H ARLWQTTE EIPKRETYCP VIVDNLIQLC KSQILKHPSL ARRICVQNST FSATWNRESL LLQVRGSGGA RLSTKDPLPT IA SREEIEA TKNHVLETFY PISPIIDLHE CNIYDVKNDT GFQEGYPYPY PHTLYLLDKA NLRPHRLQPD QLRAKMILFA FGS ALAQAR LLYGNDAKVL EQPVVVQSVG TDGRVFHFLV FQLNTTDLDC NEGVKNLAWV DSDQLLYQHF WCLPVIKKRV VVEP VGPVG FKPETFRKFL ALYLHGAA

UniProtKB: Large ribosomal subunit protein mL37

Macromolecule #14: 39S ribosomal protein L28, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L28, mitochondrial / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 30.202867 KDa

Sequence

String:

MPLHKYPVWL WKRLQLREGI CSRLPGHYLR SLEEERTPTP VHYRPHGAKF KINPKNGQRE RVEDVPIPIY FPPESQRGLW GGEGWILGQ IYANNDKLSK RLKKVWKPQL FEREFYSEIL DKKFTVTVTM RTLDLIDEAY GLDFYILKTP KEDLCSKFGM D LKRGMLLR LARQDPQLHP EDPERRAAIY DKYKEFAIPE EEAEWVGLTL EEAIEKQRLL EEKDPVPLFK IYVAELIQQL QQ QALSEPA VVQKRASGQ

UniProtKB: Large ribosomal subunit protein bL28m

Macromolecule #15: Transcription termination factor 4, mitochondrial

• Macromolecule: Name: Transcription termination factor 4, mitochondrial / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 44.012473 KDa

Sequence

String:

MAAFGRQVLD WHRLIPLTWA CMARQTPHLG EQRRTTASLL RKLTTASNGG VIEELSCVRS NNYVQEPECR RNLVQCLLEK QGTPVVQGS LELERVMSSL LDMGFSNAHI NELLSVRRGA SLQQLLDIIS EFILLGLNPE PVCVVLKKSP QLLKLPIMQM R KRSSYLQK LGLGEGKLKR VLYCCPEIFT MRQQDINDTV RLLKEKCLFT VQQVTKILHS CPSVLREDLG QLEYKFQYAY FR MGIKHPD IVKSEYLQYS LTKIKQRHIY LERLGRYQTP DKKGQTQIPN PLLKDILRVS EAEFLARTAC TSVEEFQVFK KLL AREEEE SESSTSDDKR ASLDEDEDDD DEEDNDEDDN DEDDDDEDDD EAEDNDEDED DDEEE

UniProtKB: Transcription termination factor 4, mitochondrial

Macromolecule #16: 39S ribosomal protein L38, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L38, mitochondrial / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 44.65943 KDa

Sequence

String:

MAAPWWRAAL CECRRWRGFS TSAVLGRRTP PLGPMPNSDI DLSNLERLEK YRSFDRYRRR AEQEAQAPHW WRTYREYFGE KTDPKEKID IGLPPPKVSR TQQLLERKQA IQELRANVEE ERAARLRTAS VPLDAVRAEW ERTCGPYHKQ RLAEYYGLYR D LFHGATFV PRVPLHVAYA VGEDDLMPVY CGNEVTPTEA AQAPEVTYEA EEGSLWTLLL TSLDGHLLEP DAEYLHWLLT NI PGNRVAE GQVTCPYLPP FPARGSGIHR LAFLLFKQDQ PIDFSEDARP SPCYQLAQRT FRTFDFYKKH QETMTPAGLS FFQ CRWDDS VTYIFHQLLD MREPVFEFVR PPPYHPKQKR FPHRQPLRYL DRYRDSHEPT YGIY

UniProtKB: Large ribosomal subunit protein mL38

Macromolecule #17: 39S ribosomal protein L47, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L47, mitochondrial / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.508535 KDa

Sequence

String:

MAAAGLALLC RRVSSALKSS RSLITPQVPA CTGFFLSLLP KSTPNVTSFH QYRLLHTTLS RKGLEEFFDD PKNWGQEKVK SGAAWTCQQ LRNKSNEDLH KLWYVLLKER NMLLTLEQEA KRQRLPMPSP ERLDKVVDSM DALDKVVQER EDALRLLQTG Q ERARPGAW RRDIFGRIIW HKFKQWVIPW HLNKRYNRKR FFALPYVDHF LRLEREKRAR IKARKENLER KKAKILLKKF PH LAEAQKS SLV

UniProtKB: Large ribosomal subunit protein uL29m

Macromolecule #18: Mitochondrial ribosome-associated GTPase 1

• Macromolecule: Name: Mitochondrial ribosome-associated GTPase 1 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 37.292688 KDa

Sequence

String:

MRLTPRALCS AAQAAWRENF PLCGRDVARW FPGHMAKGLK KMQSSLKLVD CIIEVHDARI PLSGRNPLFQ ETLGLKPHLL VLNKMDLAD LTEQQKIMQH LEGEGLKNVI FTNCVKDENV KQIIPMVTEL IGRSHRYHRK ENLEYCIMVI GVPNVGKSSL I NSLRRQHL RKGKATRVGG EPGITRAVMS KIQVSERPLM FLLDTPGVLA PRIESVETGL KLALCGTVLD HLVGEETMAD YL LYTLNKH QRFGYVQHYG LGSACDNVER VLKSVAVKLG KTQKVKVLTG TGNVNIIQPN YPAAARDFLQ TFRRGLLGSV MLD LDVLRG HPPAETLP

UniProtKB: Mitochondrial ribosome-associated GTPase 1

Macromolecule #19: 39S ribosomal protein L39, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L39, mitochondrial / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 38.764785 KDa

Sequence

String:

MEALAMGSRA LRLWLVAPGG GIKWRFIATS SASQLSPTEL TEMRNDLFNK EKARQLSLTP RTEKIEVKHV GKTDPGTVFV MNKNISTPY SCAMHLSEWY CRKSILALVD GQPWDMYKPL TKSCEIKFLT FKDCDPGEVN KAYWRSCAMM MGCVIERAFK D EYMVNLVR APEVPVISGA FCYDVVLDSK LDEWMPTKEN LRSFTKDAHA LIYKDLPFET LEVEAKVALE IFQHSKYKVD FI EEKASQN PERIVKLHRI GDFIDVSEGP LIPRTSICFQ YEVSAVHNLQ PTQPSLIRRF QGVSLPVHLR AHFTIWDKLL ERS RKMVTE DQSKATEECT ST

UniProtKB: Large ribosomal subunit protein mL39

Macromolecule #20: 39S ribosomal protein L30, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L30, mitochondrial / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.582812 KDa

Sequence

String:

MAGILRLVVQ WPPGRLQTVT KGVESLICTD WIRHKFTRSR IPEKVFQASP EDHEKYGGDP QNPHKLHIVT RIKSTRRRPY WEKDIIKML GLEKAHTPQV HKNIPSVNAK LKVVKHLIRI KPLKLPQGLP AEENMSNTCL KSTGELVVQW HLKPVEQKAH E S

UniProtKB: Large ribosomal subunit protein uL30m

Macromolecule #21: 39S ribosomal protein L40, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L40, mitochondrial / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.534422 KDa

Sequence

String:

MTASVLRSIS LALRPTSGLL GTWQTQLRET HQRASLLSFW ELIPMRSEPL RKKKKVDPKK DQEAKERLKR KIRKLEKATQ ELIPIEDFI TPLKFLDKAR ERPQVELTFE ETERRALLLK KWSLYKQQER KMERDTIRAM LEAQQEALEE LQLESPKLHA E AIKRDPNL FPFEKEGPHY TPPIPNYQPP EGRYNDITKV YTQVEFKR

UniProtKB: Large ribosomal subunit protein mL40

Macromolecule #22: 39S ribosomal protein L42, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L42, mitochondrial / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.691176 KDa

Sequence

String:

MAVAAVKWVM SKRTILKHLF PVQNGALYCV CHKSTYSPLP DDYNCNVELA LTSDGRTIVC YHPSVDIPYE HTKPIPRPDP VHNNEETHD QVLKTRLEEK VEHLEEGPMI EQLSKMFFTT KHRWYPHGRY HRCRKNLNPP KDR

UniProtKB: Large ribosomal subunit protein mL42

Macromolecule #23: 39S ribosomal protein L41, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L41, mitochondrial / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.404708 KDa

Sequence

String:

MGVLAAAARC LVRGADRMSK WTSKRGPRSF RGRKGRGAKG IGFLTSGWRF VQIKEMVPEF VVPDLTGFKL KPYVSYLAPE SEETPLTAA QLFSEAVAPA IEKDFKDGTF DPDNLEKYGF EPTQEGKLFQ LYPRNFLR

UniProtKB: Large ribosomal subunit protein mL41

Macromolecule #24: Large ribosomal subunit protein mL43

• Macromolecule: Name: Large ribosomal subunit protein mL43 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.352633 KDa

Sequence

String:

(ACE)TARGTPSRF LASVLHNGLG RYVQQLQRLS FSVSRDGASS RGAREFVERE VIDFARRNPG VVIYVNSRPC CVPRVV AEY LNGAVREESI HCKSVEEIST LVQKLADQSG LDVIRIRKPF HTDNPSIQGQ WHPFTNKPTT FRGLRPREVQ DPAPAQD TG LRLSAVAPQI LLPGWPDPPD LPTVDPISSS LTSAPAPMLS AVSCLPIVPA LTTVCSA

UniProtKB: Large ribosomal subunit protein mL43

Macromolecule #26: 39S ribosomal protein L44, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L44, mitochondrial / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO; EC number: Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 37.583312 KDa

Sequence

String:

MASGLVRLLQ QGHRCLLAPV APKLVPPVRG VKKGFRAAFR FQKELERQRL LRCPPPPVRR SEKPNWDYHA EIQAFGHRLQ ENFSLDLLK TAFVNSCYIK SEEAKRQQLG IEKEAVLLNL KSNQELSEQG TSFSQTCLTQ FLEDEYPDMP TEGIKNLVDF L TGEEVVCH VARNLAVEQL TLSEEFPVPP AVLQQTFFAV IGALLQSSGP ERTALFIRDF LITQMTGKEL FEMWKIINPM GL LVEELKK RNVSAPESRL TRQSGGTTAL PLYFVGLYCD KKLIAEGPGE TVLVAEEEAA RVALRKLYGF TENRRPWNYS KPK ETLRAE KSITAS

UniProtKB: Large ribosomal subunit protein mL44

Macromolecule #28: 39S ribosomal protein L45, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L45, mitochondrial / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.402918 KDa

Sequence

String:

MAAPIPQGFS CLSRFLGWWF RQPVLVTQSA AIVPVRTKKR FTPPIYQPKF KTEKEFMQHA RKAGLVIPPE KSDRSIHLAC TAGIFDAYV PPEGDARISS LSKEGLIERT ERMKKTMASQ VSIRRIKDYD ANFKIKDFPE KAKDIFIEAH LCLNNSDHDR L HTLVTEHC FPDMTWDIKY KTVRWSFVES LEPSHVVQVR CSSMMNQGNV YGQITVRMHT RQTLAIYDRF GRLMYGQEDV PK DVLEYVV FEKQLTNPYG SWRMHTKIVP PWAPPKQPIL KTVMIPGPQL KPEEEYEEAQ GEAQKPQLA

UniProtKB: Large ribosomal subunit protein mL45

Macromolecule #29: 39S ribosomal protein L2, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L2, mitochondrial / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 33.355941 KDa

Sequence

String:

MALCALTRAL RSLNLAPPTV AAPAPSLFPA AQMMNNGLLQ QPSALMLLPC RPVLTSVALN ANFVSWKSRT KYTITPVKMR KSGGRDHTG RIRVHGIGGG HKQRYRMIDF LRFRPEETKS GPFEEKVIQV RYDPCRSADI ALVAGGSRKR WIIATENMQA G DTILNSNH IGRMAVAARE GDAHPLGALP VGTLINNVES EPGRGAQYIR AAGTCGVLLR KVNGTAIIQL PSKRQMQVLE TC VATVGRV SNVDHNKRVI GKAGRNRWLG KRPNSGRWHR KGGWAGRKIR PLPPMKSYVK LPSASAQS

UniProtKB: Large ribosomal subunit protein uL2m

Macromolecule #30: 39S ribosomal protein L46, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L46, mitochondrial / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 31.748107 KDa

Sequence

String:

MAAPVRRTLL GVAGGWRRFE RLWAGSLSSR SLALAAAPSS NGSPWRLLGA LCLQRPPVVS KPLTPLQEEM ASLLQQIEIE RSLYSDHEL RALDENQRLA KKKADLHDEE DEQDILLAQD LEDMWEQKFL QFKLGARITE ADEKNDRTSL NRKLDRNLVL L VREKFGDQ DVWILPQAEW QPGETLRGTA ERTLATLSEN NMEAKFLGNA PCGHYTFKFP QAMRTESNLG AKVFFFKALL LT GDFSQAG NKGHHVWVTK DELGDYLKPK YLAQVRRFVS DL

UniProtKB: Large ribosomal subunit protein mL46

Macromolecule #31: 39S ribosomal protein L3, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L3, mitochondrial / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 38.689562 KDa

Sequence

String:

MPGWRLLTQV GAQVLGRLGD GLGAALGPGN RTHIWLFVRG LHGKSGTWWD EHLSEENVPF IKQLVSDEDK AQLASKLCPL KDEPWPIHP WEPGSFRVGL IALKLGMMPL WTKDGQKHVV TLLQVQDCHV LKYTSKENCN GKMATLSVGG KTVSRFRKAT S ILEFYREL GLPPKQTVKI FNITDNAAIK PGTPLYAAHF RPGQYVDVTA KTIGKGFQGV MKRWGFKGQP ATHGQTKTHR RP GAVATGD IGRVWPGTKM PGKMGNIYRT EYGLKVWRIN TKHNIIYVNG SVPGHKNCLV KVKDSKLPAY KDLGKNLPFP TYF PDGDEE ELPEDLYDEN VCQPGAPSIT FA

UniProtKB: Large ribosomal subunit protein uL3m

Macromolecule #32: 39S ribosomal protein L48, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L48, mitochondrial / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.970814 KDa

Sequence

String:

MSGTLEKVLC LRNNTIFKQA FSLLRFRTSG EKPIYSVGGI LLSISRPYKT KPTHGIGKYK HLIKAEEPKK KKGKVEVRAI NLGTDYEYG VLNIHLTAYD MTLAESYAQY VHNLCNSLSI KVEESYAMPT KTIEVLQLQD QGSKMLLDSV LTTHERVVQI S GLSATFAE IFLEIIQSSL PEGVRLSVKE HTEEDFKGRF KARPELEELL AKLK

UniProtKB: Large ribosomal subunit protein mL48

Macromolecule #33: 39S ribosomal protein L4, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L4, mitochondrial / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.970195 KDa

Sequence

String:

MLQFVRAGAR AWLRPTGSQG LSSLAEEAAR ATENPEQVAS EGLPEPVLRK VELPVPTHRR PVQAWVESLR GFEQERVGLA DLHPDVFAT APRLDILHQV AMWQKNFKRI SYAKTKTRAE VRGGGRKPWP QKGTGRARHG SIRSPLWRGG GVAHGPRGPT S YYYMLPMK VRALGLKVAL TVKLAQDDLH IMDSLELPTG DPQYLTELAH YRRWGDSVLL VDLTHEEMPQ SIVEATSRLK TF NLIPAVG LNVHSMLKHQ TLVLTLPTVA FLEDKLLWQD SRYRPLYPFS LPYSDFPRPL PHATQGPAAT PYHC

UniProtKB: Large ribosomal subunit protein uL4m

Macromolecule #34: 39S ribosomal protein L49, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L49, mitochondrial / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 19.225043 KDa

Sequence

String:

MAATMFRATL RGWRTGVQRG CGLRLLSQTQ GPPDYPRFVE SVDEYQFVER LLPATRIPDP PKHEHYPTPS GWQPPRDPPP NLPYFVRRS RMHNIPVYKD ITHGNRQMTV IRKVEGDIWA LQKDVEDFLS PLLGKTPVTQ VNEVTGTLRI KGYFDQELKA W LLEKGF

UniProtKB: Large ribosomal subunit protein mL49

Macromolecule #35: 39S ribosomal protein L9, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L9, mitochondrial / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 30.296355 KDa

Sequence

String:

MAAPVVTAPG RALLRAGAGR LLRGGVQELL RPRHEGNAPD LACNFSLSQN RGTVIVERWW KVPLAGEGRK PRLHRRHRVY KLVEDTKHR PKENLELILT QSVENVGVRG DLVSVKKSLG RNRLLPQGLA VYASPENKKL FEEEKLLRQE GKLEKIQTKA G EATVKFLK SCRLEVGMKN NVKWELNPEI VARHFFKNLG VVVAPHTLKL PEEPITRWGE YWCEVTVNGL DTVRVPMSVV NF EKPKTKR YKYWLAQQAA KAMAPTSPQI

UniProtKB: Large ribosomal subunit protein bL9m

Macromolecule #36: 39S ribosomal protein L50, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L50, mitochondrial / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.349943 KDa

Sequence

String:

MAARSVSGIT RRVFMWTVSG TPCREFWSRF RKEKEPVVVE TVEEKKEPIL VCPPLRSRAY TPPEDLQSRL ESYVKEVFGS SLPSNWQDI SLEDSRLKFN LLAHLADDLG HVVPNSRLHQ MCRVRDVLDF YNVPIQDRSK FDELSASNLP PNLKITWSY

UniProtKB: Large ribosomal subunit protein mL50

Macromolecule #37: 39S ribosomal protein L10, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L10, mitochondrial / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.322529 KDa

Sequence

String:

MAAAVAGMLR GGLLPQAGRL PTLQTVRYGS KAVTRHRRVM HFQRQKLMAV TEYIPPKPAI HPSCLPSPPS PPQEEIGLIR LLRREIAAV FQDNRMIAVC QNVALSAEDK LLMRHQLRKH KILMKVFPNQ VLKPFLEDSK YQNLLPLFVG HNMLLVSEEP K VKEMVRIL RTVPFLPLLG GCIDDTILSR QGFINYSKLP SLPLVQGELV GGLTCLTAQT HSLLQHQPLQ LTTLLDQYIR EQ REKDSVM SANGKPDPDT VPDS

UniProtKB: Large ribosomal subunit protein uL10m

Macromolecule #38: 39S ribosomal protein L51, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L51, mitochondrial / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.125917 KDa

Sequence

String:

MAGNLLSGAG RRLWDWVPLA CRSFSLGVPR LIGIRLTLPP PKVVDRWNEK RAMFGVYDNI GILGNFEKHP KELIRGPIWL RGWKGNELQ RCIRKRKMVG SRMFADDLHN LNKRIRYLYK HFNRHGKFR

UniProtKB: Large ribosomal subunit protein mL51

Macromolecule #39: 39S ribosomal protein L11, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L11, mitochondrial / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 20.716227 KDa

Sequence

String:

MSKLGRAARG LRKPEVGGVI RAIVRAGLAM PGPPLGPVLG QRGVSINQFC KEFNERTKDI KEGIPLPTKI LVKPDRTFEI KIGQPTVSY FLKAAAGIEK GARQTGKEVA GLVTLKHVYE IARIKAQDEA FALQDVPLSS VVRSIIGSAR SLGIRVVKDL S SEELAAFQ KERAIFLAAQ KEADLAAQEE AAKK

UniProtKB: Large ribosomal subunit protein uL11m

Macromolecule #40: 39S ribosomal protein L52, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L52, mitochondrial / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.682658 KDa

Sequence

String:

MAALGTVLFT GVRRLHCSVA AWAGGQWRLQ QGLAANPSGY GPLTELPDWS YADGRPAPPM KGQLRRKAER ETFARRVVLL SQEMDAGLQ AWQLRQQKLQ EEQRKQENAL KPKGASLKSP LPSQ

UniProtKB: Large ribosomal subunit protein mL52

Macromolecule #41: 39S ribosomal protein L13, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L13, mitochondrial / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 20.765023 KDa

Sequence

String:

M(SAC)SFSRAPQQ WATFARIWYL LDGKMQPPGK LAAMASIRLQ GLHKPVYHAL SDCGDHVVIM NTRHIAFSGN KWEQKV YSS HTGYPGGFRQ VTAAQLHLRD PVAIVKLAIY GMLPKNLHRR TMMERLHLFP DEYIPEDILK NLVEELPQPR KIPKRLD EY TQEEIDAFPR LWTPPEDYRL

UniProtKB: Large ribosomal subunit protein uL13m

Macromolecule #42: 39S ribosomal protein L53, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L53, mitochondrial / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 12.167896 KDa

Sequence

String:

M(AYA)AALARLGL RPVKQVRVQF CPFEKNVEST RTFLQTVSSE KVRSTNLNCS VIADVRHDGS EPCVDVLFGD GHRLIM RGA HLTALEMLTA FASHIRARDA AGSGDKPGAD TGR

UniProtKB: Large ribosomal subunit protein mL53

Macromolecule #43: 39S ribosomal protein L14, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L14, mitochondrial / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.973702 KDa

Sequence

String:

MAFFTGLWGP FTCVSRVLSH HCFSTTGSLS AIQKMTRVRV VDNSALGNSP YHRAPRCIHV YKKNGVGKVG DQILLAIKGQ KKKALIVGH CMPGPRMTPR FDSNNVVLIE DNGNPVGTRI KTPIPTSLRK REGEYSKVLA IAQNFV

UniProtKB: Large ribosomal subunit protein uL14m

Macromolecule #44: 39S ribosomal protein L54, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L54, mitochondrial / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.844178 KDa

Sequence

String:

MATKRLFGAT RTWAGWGAWE LLNPATSGRL LARDYAKKPV MKGAKSGKGA VTSEALKDPD VCTDPVQLTT YAMGVNIYKE GQDVPLKPD AEYPEWLFEM NLGPPKTLEE LDPESREYWR RLRKQNIWRH NRLSKNKRL

UniProtKB: Large ribosomal subunit protein mL54

Macromolecule #45: 39S ribosomal protein L15, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L15, mitochondrial / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 33.47382 KDa

Sequence

String:

MAGPLQGGGA RALDLLRGLP RVSLANLKPN PGSKKPERRP RGRRRGRKCG RGHKGERQRG TRPRLGFEGG QTPFYIRIPK YGFNEGHSF RRQYKPLSLN RLQYLIDLGR VDPSQPIDLT QLVNGRGVTI QPLKRDYGVQ LVEEGADTFT AKVNIEVQLA S ELAIAAIE KNGGVVTTAF YDPRSLDIVC KPVPFFLRGQ PIPKRMLPPE ELVPYYTDAK NRGYLADPAK FPEARLELAR KY GYILPDI TKDELFKMLC TRKDPRQIFF GLAPGWVVNM ADKKILKPTD ENLLKYYTS

UniProtKB: Large ribosomal subunit protein uL15m

Macromolecule #46: 39S ribosomal protein L55, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L55, mitochondrial / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.160433 KDa

Sequence

String:

MAAVGSLLGR LRQSTVKATG PALRRLHTSS WRADSSRASL TRVHRQAYAR LYPVLLVKQD GSTIHIRYRE PRRMLAMPID LDTLSPEER RARLRKREAQ LQSRKEYEQE LSDDLHVERY RQFWTRTKK

UniProtKB: Large ribosomal subunit protein mL55

Macromolecule #47: 39S ribosomal protein L16, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L16, mitochondrial / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.496285 KDa

Sequence

String:

MWRLLARASA PLLRVPLSDS WALLPASAGV KTLLPVPSFE DVSIPEKPKL RFIERAPLVP KVRREPKNLS DIRGPSTEAT EFTEGNFAI LALGGGYLHW GHFEMMRLTI NRSMDPKNMF AIWRVPAPFK PITRKSVGHR MGGGKGAIDH YVTPVKAGRL V VEMGGRCE FEEVQGFLDQ VAHKLPFAAK AVSRGTLEKM RKDQEERERN NQNPWTFERI ATANMLGIRK VLSPYDLTHK GK YWGKFYM PKRV

UniProtKB: Large ribosomal subunit protein uL16m

Macromolecule #48: Ribosomal protein 63, mitochondrial

• Macromolecule: Name: Ribosomal protein 63, mitochondrial / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 12.292333 KDa

Sequence

String:

MFLTALLWRG RIPGRQWIGK HRRPRFVSLR AKQNMIRRLE IEAENHYWLS MPYMTREQER GHAAVRRREA FEAIKAAATS KFPPHRFIA DQLDHLNVTK KWS

UniProtKB: Large ribosomal subunit protein mL63

Macromolecule #49: 39S ribosomal protein L17, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L17, mitochondrial / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 20.083266 KDa

Sequence

String:

MRLSVAAAIS HGRVFRRMGL GPESRIHLLR NLLTGLVRHE RIEAPWARVD EMRGYAEKLI DYGKLGDTNE RAMRMADFWL TEKDLIPKL FQVLAPRYKD QTGGYTRMLQ IPNRSLDRAK MAVIEYKGNC LPPLPLPRRD SHLTLLNQLL QGLRQDLRQS Q EASNHSSH TAQTPGI

UniProtKB: Large ribosomal subunit protein bL17m

Macromolecule #50: Peptidyl-tRNA hydrolase ICT1, mitochondrial

• Macromolecule: Name: Peptidyl-tRNA hydrolase ICT1, mitochondrial / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidyl-tRNA hydrolase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.674203 KDa

Sequence

String:

MAATRCLRWG LSRAGVWLLP PPARCPRRAL HKQKDGTEFK SIYSLDKLYP ESQGSDTAWR VPNGAKQADS DIPLDRLTIS YCRSSGPGG QNVNKVNSKA EVRFHLATAE WIAEPVRQKI AITHKNKINR LGELILTSES SRYQFRNLAD CLQKIRDMIT E ASQTPKEP TKEDVKLHRI RIENMNRERL RQKRIHSAVK TSRRVDMD

UniProtKB: Large ribosomal subunit protein mL62

Macromolecule #51: 39S ribosomal protein L18, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L18, mitochondrial / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 20.60757 KDa

Sequence

String:

MALRSRFWGL FSVCRNPGCR FAALSTSSEP AAKPEVDPVE NEAVAPEFTN RNPRNLELLS VARKERGWRT VFPSREFWHR LRVIRTQHH VEALVEHQNG KVVVSASTRE WAIKKHLYST RNVVACESIG RVLAQRCLEA GINFMVYQPT PWEAASDSMK R LQSAMTEG GVVLREPQRI YE

UniProtKB: Large ribosomal subunit protein uL18m

Macromolecule #52: Growth arrest and DNA damage-inducible proteins-interacting protein 1

• Macromolecule: Name: Growth arrest and DNA damage-inducible proteins-interacting protein 1; type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 25.426895 KDa

Sequence

String:

MAASVRQARS LLGVAATLAP GSRGYRARPP PRRRPGPRWP DPEDLLTPRW QLGPRYAAKQ FARYGAASGV VPGSLWPSPE QLRELEAEE REWYPSLATM QESLRVKQLA EEQKRREREQ HIAECMAKMP QMIVNWQQQQ RENWEKAQAD KERRARLQAE A QELLGYQV DPRSARFQEL LQDLEKKERK RLKEEKQKRK KEARAAALAA AVAQDPAASG APSS

UniProtKB: Large ribosomal subunit protein mL64

Macromolecule #53: 39S ribosomal protein L19, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L19, mitochondrial / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 33.584996 KDa

Sequence

String:

MAACIAAGHW AAMGLGRSFQ AARTLLPPPA SIACRVHAGP VRQQSTGPSE PGAFQPPPKP VIVDKHRPVE PERRFLSPEF IPRRGRTDP LKFQIERKDM LERRKVLHIP EFYVGSILRV TTADPYASGK ISQFLGICIQ RSGRGLGATF ILRNVIEGQG V EICFELYN PRVQEIQVVK LEKRLDDSLL YLRDALPEYS TFDVNMKPVV QEPNQKVPVN ELKVKMKPKP WSKRWERPNF NI KGIRFDL CLTEQQMKEA QKWNQPWLEF DMMREYDTSK IEAAIWKEIE ASKRS

UniProtKB: Large ribosomal subunit protein bL19m

Macromolecule #54: 39S ribosomal protein S18a, mitochondrial

• Macromolecule: Name: 39S ribosomal protein S18a, mitochondrial / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.224078 KDa

Sequence

String:

MAALKALVSG CGRLLRGLLA GPAATSWSRL PARGFREVVE TQEGKTTIIE GRITATPKES PNPPNPSGQC PICRWNLKHK YNYDDVLLL SQFIRPHGGM LPRKITGLCQ EEHRKIEECV KMAHRAGLLP NHRPRLPEGV VPKSKPQLNR YLTRWAPGSV K PIYKKGPR WNRVRMPVGS PLLRDNVCYS RTPWKLYH

UniProtKB: Large ribosomal subunit protein mL66

Macromolecule #55: 39S ribosomal protein L20, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L20, mitochondrial / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.479654 KDa

Sequence

String:

MVFLTAQLWL RNRVTDRYFR IQEVLKHARH FRGRKNRCYR LAVRTVIRAF VKCTKARYLK KKNMRTLWIN RITAASQEHG LKYPALIGN LVKCQVELNR KVLADLAIYE PKTFKSLAAL ASRRRHEGFA AALGDGKEPE GIFSRVVQYH

UniProtKB: Large ribosomal subunit protein bL20m

Macromolecule #56: 39S ribosomal protein S30, mitochondrial

• Macromolecule: Name: 39S ribosomal protein S30, mitochondrial / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 50.429578 KDa

Sequence

String:

MAAARCWRPL LRGPRLSLHT AANAAATATE TTCQDVAATP VARYPPIVAS MTADSKAARL RRIERWQATV HAAESVDEKL RILTKMQFM KYMVYPQTFA LNADRWYQYF TKTVFLSGLP PPPAEPEPEP EPEPEPALDL AALRAVACDC LLQEHFYLRR R RRVHRYEE SEVISLPFLD QLVSTLVGLL SPHNPALAAA ALDYRCPVHF YWVRGEEIIP RGHRRGRIDD LRYQIDDKPN NQ IRISKQL AEFVPLDYSV PIEIPTIKCK PDKLPLFKRQ YENHIFVGSK TADPCCYGHT QFHLLPDKLR RERLLRQNCA DQI EVVFRA NAIASLFAWT GAQAMYQGFW SEADVTRPFV SQAVITDGKY FSFFCYQLNT LALTTQADQN NPRKNICWGT QSKP LYETI EDNDVKGFND DVLLQIVHFL LNRPKEEKSQ LLEN

UniProtKB: Large ribosomal subunit protein mL65

Macromolecule #57: 39S ribosomal protein L21, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L21, mitochondrial / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.847682 KDa

Sequence

String:

MAASSLTVTL GRLASACSHS ILRPSGPGAA SLWSASRRFN SQSTSYLPGY VPKTSLSSPP WPEVVLPDPV EETRHHAEVV KKVNEMIVT GQYGRLFAVV HFASRQWKVT SEDLILIGNE LDLACGERIR LEKVLLVGAD NFTLLGKPLL GKDLVRVEAT V IEKTESWP RIIMRFRKRK NFKKKRIVTT PQTVLRINSI EIAPCLL

UniProtKB: Large ribosomal subunit protein bL21m

Macromolecule #58: GTP-binding protein 10

• Macromolecule: Name: GTP-binding protein 10 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 42.996895 KDa

Sequence

String:

MVHCSCVLFR KYGNFIDKLR LFTRGGSGGM GYPRLGGEGG KGGDVWVVAQ NRMTLKQLKD RYPRKRFVAG VGANSKISAL KGSKGKDCE IPVPVGISVT DENGKIIGEL NKENDRILVA QGGLGGKLLT NFLPLKGQKR IIHLDLKLIA DVGLVGFPNA G KSSLLSCV SHAKPAIADY AFTTLKPELG KIMYSDFKQI SVADLPGLIE GAHMNKGMGH KFLKHIERTR QLLFVVDISG FQ LSSHTQY RTAFETIILL TKELELYKEE LQTKPALLAV NKMDLPDAQD KFHELMSQLQ NPKDFLHLFE KNMIPERTVE FQH IIPISA VTGEGIEELK NCIRKSLDEQ ANQENDALHK KQLLNLWISD TMSSTEPPSK HAVTTSKMDI I

UniProtKB: GTP-binding protein 10

Macromolecule #25: 16S rRNA + pre-H68-71 segment

• Macromolecule: Name: 16S rRNA + pre-H68-71 segment / type: rna / ID: 25 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 509.550594 KDa

Sequence

String:

GCUAAACCUA GCCCCAAACC CACUCCACCU UACUACCAGA CAACCUUAGC CAAACCAUUU ACCCAAAUAA AGUAUAGGCG AUAGAAAUU GAAACCUGGC GCAAUAGAUA UAGUACCGCA AGGGAAAGAU GAAAAAUUAU AACCAAGCAU AAUAUAGCAA G GACUAACC CCUAUACCUU CUGCAUAAUG AAUUAACUAG AAAUAACUUU GCAAGGAGAG CCAAAGCUAA GACCCCCGAA AC CAGACGA GCUACCUAAG AACAGCUAAA AGAGCACACC CGUCUAUGUA GCAAAAUAGU GGGAAGAUUU AUAGGUAGAG GCG ACAAAC CUACCGAGCC UGGUGAUAGC UGGUUGUCCA AGAUAGAAUC UUAGUUCAAC UUUAAAUUUG CCCACAGAAC CCUC UAAAU CCCCUUGUAA AUUUAACUGU UAGUCCAAAG AGGAACAGCU CUUUGGACAC UAGGAAAAAA CCUUGUAGAG AGAGU AAAA AAUUUAACAC CCAUAGUAGG CCUAAAAGCA GCCACCAAUU AAGAAAGCGU UCAAGCUCAA CACCCACUAC CUAAAA AAU CCCAAACAUA UAACUGAACU CCUCACACCC AAUUGGACCA AUCUAUCACC CUAUAGAAGA ACUAAUGUUA GUAUAAG UA ACAUGAAAAC AUUCUCCUCC GCAUAAGCCU GCGUCAGAUU AAAACACUGA ACUGACAAUU AACAGCCCAA UAUCUACA A UCAACCAACA AGUCAUUAUU ACCCUCACUG UCAACCCAAC ACAGGCAUGC UCAUAAGGAA AGGUUAAAAA AAGUAAAAG GAACUCGGCA AAUCUUACCC CGCCUGUUUA CCAAAAACAU CACCUCUAGC AUCACCAGUA UUAGAGGCAC CGCCUGCUAU AUAUAUAUA UAUAUAUAUA UAUAUAUAUC CAGUGACACA UGUUUAACGG CCGCGGUACC CUAACCGUGC AAAGGUAGCA U AAUCACUU GUUCCUUAAA UAGGGACCUG UAUGAAUGGC UCCACGAGGG UUCAGCUGUC UCUUACUUUU AACCAGUGAA AU UGACCUG CCCGUGAAGA GGCGGGCAUA ACACAGCAAG ACGAGAAGAC CCUAUGGAGC UUUAAUUUAU UAAUGCAAAC AGU ACCUAA CAAACCCACA GGUCCUAAAC UACCAAACCU GCAUUAAAAA UUUCGGUUGG GGCGACCUCG GAGCAGAACC CAAC CUCCG AGCAGUACAU GCUAAGACUU CACCAGUCAA AGCGAACUAC UAUACUCAAU UGAUCCAAUA ACUUGACCAA CGGAA CAAG UUACCCUAGG GAUAACAGCG CAAUCCUAUU CUAGAGUCCA UAUCAACAAU AGGGUUUACG ACCUCGAUGU UGGAUC AGG ACAUCCCGAU GGUGCAGCCG CUAUUAAAGG UUCGUUUGUU CAACGAUUAA AGUCCUACGU GAUCUGAGUU CAGACCG GA GUAAUCCAGG UCGGUUUCUA UCUACUUUCA AAUUCCUCCC UGUACGAAAG GACAAGAGAA AUAAGGCCUA CUUCACAA A GCGCCUUCCC CCGUAAAUGA UAUCAUCUCA ACUUAGUAUU AUACCCACAC CCACCCAAGA ACAGGGUU

GENBANK: GENBANK: MN849737.1, GENBANK: MN849737.1

Macromolecule #27: CP tRNA-Val

• Macromolecule: Name: CP tRNA-Val / type: rna / ID: 27 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.961699 KDa

Sequence

String:

CAGAGUGUAG CUUAACACAA AGCACCCAAC UUACACUUAG GAGAUUUCAA CUUAACUUGA CCGCUCUGAC CA

GENBANK: GENBANK: LC530724.1

Macromolecule #59: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 59 / Number of copies: 1 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #60: S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYL...

• Macromolecule: Name: S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE; type: ligand / ID: 60 / Number of copies: 1 / Formula: PM8
• Molecular weight: Theoretical: 496.598 Da

Chemical component information

ChemComp-PM8:
S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE

Macromolecule #61: S-ADENOSYLMETHIONINE

• Macromolecule: Name: S-ADENOSYLMETHIONINE / type: ligand / ID: 61 / Number of copies: 1 / Formula: SAM
• Molecular weight: Theoretical: 398.437 Da

Chemical component information

ChemComp-SAM:
S-ADENOSYLMETHIONINE

Macromolecule #62: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER

• Macromolecule: Name: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / type: ligand / ID: 62 / Number of copies: 2 / Formula: GNP
• Molecular weight: Theoretical: 522.196 Da

Chemical component information

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM

Macromolecule #63: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 63 / Number of copies: 96 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #64: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 64 / Number of copies: 10 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.6
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Average electron dose: 40.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.7000000000000001 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

7odt

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.03 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 31656
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD