[English] 日本語
![](img/lk-miru.gif)
- EMDB-17467: Subtomogram representing a segment of the outer wall of empty ger... -
+
Open data
-
Basic information
Entry | ![]() | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Subtomogram representing a segment of the outer wall of empty germinated polar tubes from Vairimorpha necatrix. | |||||||||
![]() | Merged map after refinement. | |||||||||
![]() |
| |||||||||
![]() | Polar tube / Subtomogram / ![]() | |||||||||
Biological species | ![]() | |||||||||
Method | subtomogram averaging / ![]() | |||||||||
![]() | Sharma H / Ehrenbolger K / Jespersen N / Carlson LA / Barandun J | |||||||||
Funding support | European Union, 2 items
| |||||||||
![]() | ![]() Title: Ribosome clustering and surface layer reorganization in the microsporidian host-invasion apparatus Authors: Sharma H / Jespersen N / Ehrenbolger K / Carlson LA / Barandun J | |||||||||
History |
|
-
Structure visualization
Supplemental images |
---|
-
Downloads & links
-EMDB archive
Map data | ![]() | 118.4 KB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 15.8 KB 15.8 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 1.6 KB | Display | ![]() |
Images | ![]() | 39.8 KB | ||
Masks | ![]() | 129 KB | ![]() | |
Filedesc metadata | ![]() | 4.4 KB | ||
Others | ![]() ![]() | 118.7 KB 118.7 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
EMDB pages | ![]() ![]() |
---|
-
Map
File | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Merged map after refinement. | ||||||||||||||||||||
Voxel size | X=Y=Z: 8.69 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Mask #1
File | ![]() | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: Half map 1 from refinement.
File | emd_17467_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Half map 1 from refinement. | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: Half map 1 from refinement.
File | emd_17467_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Half map 1 from refinement. | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-
Sample components
-Entire : Subtomogram representing a segment of the outer wall of cargo-fil...
Entire | Name: Subtomogram representing a segment of the outer wall of cargo-filled, germinated polar tubes from Vairimorpha necatrix. |
---|---|
Components |
|
-Supramolecule #1: Subtomogram representing a segment of the outer wall of cargo-fil...
Supramolecule | Name: Subtomogram representing a segment of the outer wall of cargo-filled, germinated polar tubes from Vairimorpha necatrix. type: organelle_or_cellular_component / ID: 1 / Parent: 0 |
---|---|
Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | ![]() |
---|---|
![]() | subtomogram averaging |
Aggregation state | filament |
-
Sample preparation
Buffer | pH: 8 Component:
Details: Germination buffer (0.17 mM KCl, 1 mM Tris-HCl (pH 8.0), 10 mM EDTA) | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Grid | Model: PELCO Ultrathin Carbon with Lacey Carbon / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: LACEY / Support film - Film thickness: 200 | ||||||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV | ||||||||||||
Details | Subtomogram representing a segment of the outer wall of cargo-filled, germinated polar tubes from Vairimorpha nectrix. |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: OTHER / Nominal defocus max: 5.0 µm / Nominal defocus min: 1.5 µm |
Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Detector mode: COUNTING / Average exposure time: 1.0 sec. / Average electron dose: 2.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
-
Image processing
-Atomic model buiding 1
Refinement | Protocol: OTHER |
---|