EMDB-17367: Ternary complex of translating ribosome, NAC and METAP1

Basic information

Entry

Database: EMDB / ID: EMD-17367

• Title: Ternary complex of translating ribosome, NAC and METAP1

Sample

• Complex: Ternary complex of translating ribosome, NAC and METAP1
  • Complex: complex of NAC and METAP1 at the tennel exit of translating ribosome
    • Protein or peptide: x 3 types
  • Complex: Ribosome translating a cytosolic peptide
    • RNA: x 6 types
    • Protein or peptide: x 79 types
• Ligand: x 9 types

• Keywords: ribosome / methionine aminopeptidase 1 / methionine excision / protein biogenesis / nascent chain / TRANSLATION

Function / homology

Function:

negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / negative regulation of striated muscle cell apoptotic process / regulation of skeletal muscle fiber development / positive regulation of cell proliferation involved in heart morphogenesis / positive regulation of skeletal muscle tissue growth / cardiac ventricle development / glucose-6-phosphate isomerase / N-terminal protein amino acid modification / glucose-6-phosphate isomerase activity / peptidyl-methionine modification / hemostasis / glucose 6-phosphate metabolic process / methionyl aminopeptidase / initiator methionyl aminopeptidase activity / heart trabecula morphogenesis / carbohydrate derivative binding / skeletal muscle tissue regeneration / metalloexopeptidase activity / Gluconeogenesis / monosaccharide binding / fructose 6-phosphate metabolic process / ribosomal subunit / Glycolysis / exit from mitosis / erythrocyte homeostasis / ciliary membrane / optic nerve development / positive regulation of immunoglobulin production / laminin receptor activity / retinal ganglion cell axon guidance / organelle membrane / metalloaminopeptidase activity / response to testosterone / humoral immune response / Peptide chain elongation / mesoderm formation / Selenocysteine synthesis / response to immobilization stress / positive regulation of signal transduction by p53 class mediator / Formation of a pool of free 40S subunits / ubiquitin ligase inhibitor activity / Eukaryotic Translation Termination / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / Viral mRNA Translation / phagocytic cup / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / 90S preribosome / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / aminopeptidase activity / Major pathway of rRNA processing in the nucleolus and cytosol / protein-RNA complex assembly / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / response to cadmium ion / maturation of LSU-rRNA / ribosomal small subunit export from nucleus / rough endoplasmic reticulum / laminin binding / translation regulator activity / gastrulation / response to muscle stretch / MDM2/MDM4 family protein binding / cytosolic ribosome / positive regulation of endothelial cell migration / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / protein maturation / cellular response to interleukin-4 / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / response to progesterone / ribosomal large subunit biogenesis / positive regulation of apoptotic signaling pathway / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / cytokine activity / gluconeogenesis / glycolytic process / TP53 Regulates Metabolic Genes / maturation of SSU-rRNA / small-subunit processome / growth factor activity / wound healing / spindle / platelet aggregation / mRNA 5'-UTR binding / Regulation of expression of SLITs and ROBOs / cytoplasmic ribonucleoprotein granule / antimicrobial humoral immune response mediated by antimicrobial peptide / rRNA processing / rhythmic process / positive regulation of canonical Wnt signaling pathway / Inactivation, recovery and regulation of the phototransduction cascade / glucose homeostasis / protein transport / response to estradiol / regulation of translation / large ribosomal subunit / heparin binding / ribosome binding

Domain/homology:

Transcription factor BTF3 / Nascent polypeptide-associated complex NAC domain / Nascent polypeptide-associated complex subunit alpha / NAC A/B domain superfamily / NAC domain / NAC A/B domain profile. / NAC / Nascent polypeptide-associated complex subunit alpha-like, UBA domain / HYPK UBA domain / MYND-like zinc finger / zf-MYND-like zinc finger, mRNA-binding / Zinc finger C6H2-type profile. / Phosphoglucose isomerase, C-terminal / Phosphoglucose isomerase signature 1. / Phosphoglucose isomerase (PGI) / Phosphoglucose isomerase, conserved site / Phosphoglucose isomerase, SIS domain 1 / Phosphoglucose isomerase, SIS domain 2 / Phosphoglucose isomerase / Phosphoglucose isomerase signature 2. / Glucose-6-phosphate isomerase family profile. / Methionine aminopeptidase subfamily 1 signature. / Peptidase M24A, methionine aminopeptidase, subfamily 1 / SIS domain superfamily / Peptidase M24, methionine aminopeptidase / 60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / : / 40S ribosomal protein SA / Peptidase M24 / Metallopeptidase family M24 / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / Creatinase/aminopeptidase-like / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ubiquitin-like protein FUBI / Ribosomal protein L30e / Ribosomal protein L2, archaeal-type / Ribosomal L15/L27a, N-terminal / Ribosomal protein L28e / 50S ribosomal protein L10, insertion domain superfamily / Ribosomal protein L23 / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / metallochaperone-like domain / TRASH domain / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein L1, conserved site / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein L1 signature. / : / Ribosomal protein S12e signature. / Ribosomal protein L1 / Ribosomal protein S12e / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / S27a-like superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L10e / 40S Ribosomal protein S10 / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / : / Ribosomal protein S7e signature. / Ribosomal protein L44e signature. / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / : / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L19, eukaryotic / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature.

Component:

Small ribosomal subunit protein eS32 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein eS21 / Large ribosomal subunit protein eL20 / Ubiquitin-ribosomal protein eL40 fusion protein / 40S ribosomal protein S26 / Transcription factor BTF3 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL15/eL18 domain-containing protein / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL33 / Small ribosomal subunit protein eS12 / Large ribosomal subunit protein eL29 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein RACK1 / Ubiquitin-ribosomal protein eS31 fusion protein / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein uL1 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein eS7 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL43 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL14 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein eL15 / 40S ribosomal protein S24 / Large ribosomal subunit protein uL14 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein eS25 / Large ribosomal subunit protein eL30 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein eS28 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein eS4 / Large ribosomal subunit protein eL13 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein eS19 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein eS10 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein eL22 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein eL36 / Small ribosomal subunit protein eS17 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein eL27 / Large ribosomal subunit protein eL34 / Large ribosomal subunit protein eL19 / Small ribosomal subunit protein eS27 / Large ribosomal subunit protein eL38 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein eL42 / Large ribosomal subunit protein eL32 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein eL28 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS5 / Glucose-6-phosphate isomerase / Methionine aminopeptidase 1 / Large ribosomal subunit protein eL8 / Nascent polypeptide-associated complex subunit alpha / Large ribosomal subunit protein eL37

• Biological species: Oryctolagus cuniculus (rabbit) / Homo sapiens (human)
• Method: single particle reconstruction / cryo EM / Resolution: 2.9 Å
• Authors: Jia M / Jaskolowski M / Scaiola A / Jomaa A / Ban N

Funding support

Switzerland, Germany, 4 items

Organization	Grant number	Country
Swiss National Science Foundation	51NF40_141735	Switzerland
Swiss National Science Foundation	310030_212308	Switzerland
German Research Foundation (DFG)	SFB969/A01	Germany
German Research Foundation (DFG)	SFB969/A07	Germany

• Citation: Journal: Science / Year: 2023; Title: NAC controls cotranslational N-terminal methionine excision in eukaryotes.; Authors: Martin Gamerdinger / Min Jia / Renate Schloemer / Laurenz Rabl / Mateusz Jaskolowski / Katrin M Khakzar / Zeynel Ulusoy / Annalena Wallisch / Ahmad Jomaa / Gundula Hunaeus / Alain Scaiola / Kay Diederichs / Nenad Ban / Elke Deuerling / ; Abstract: N-terminal methionine excision from newly synthesized proteins, catalyzed cotranslationally by methionine aminopeptidases (METAPs), is an essential and universally conserved process that plays a key role in cell homeostasis and protein biogenesis. However, how METAPs interact with ribosomes and how their cleavage specificity is ensured is unknown. We discovered that in eukaryotes the nascent polypeptide-associated complex (NAC) controls ribosome binding of METAP1. NAC recruits METAP1 using a long, flexible tail and provides a platform for the formation of an active methionine excision complex at the ribosomal tunnel exit. This mode of interaction ensures the efficient excision of methionine from cytosolic proteins, whereas proteins targeted to the endoplasmic reticulum are spared. Our results suggest a broader mechanism for how access of protein biogenesis factors to translating ribosomes is controlled.

History

Deposition	May 16, 2023	-
Header (metadata) release	Jul 19, 2023	-
Map release	Jul 19, 2023	-
Update	Apr 24, 2024	-
Current status	Apr 24, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_17367.map.gz / Format: CCP4 / Size: 311.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.2505 Å

Density

Contour Level	By AUTHOR: 0.75
Minimum - Maximum	-1.9221802 - 5.3502765
Average (Standard dev.)	0.010039335 (±0.22109382)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 434 434 434 Spacing 434 434 434
Cell	A=B=C: 542.717 Å α=β=γ: 90.0 °

Supplemental data

Half map: #2

• File: emd_17367_half_map_1.map

Half map: #1

• File: emd_17367_half_map_2.map

Sample components

Entire : Ternary complex of translating ribosome, NAC and METAP1

• Entire: Name: Ternary complex of translating ribosome, NAC and METAP1

Components

• Complex: Ternary complex of translating ribosome, NAC and METAP1
  • Complex: complex of NAC and METAP1 at the tennel exit of translating ribosome
    • Protein or peptide: Methionine aminopeptidase 1
    • Protein or peptide: Nascent polypeptide-associated complex subunit alpha
    • Protein or peptide: Transcription factor BTF3
  • Complex: Ribosome translating a cytosolic peptide
    • RNA: 28S rRNA
    • RNA: 5S rRNA
    • RNA: 5.8S rRNA
    • Protein or peptide: 60S ribosomal protein L8
    • Protein or peptide: Ribosomal protein L3
    • Protein or peptide: 60S ribosomal protein L4
    • Protein or peptide: Ribosomal protein L5 eukaryotic C-terminal domain-containing protein
    • Protein or peptide: 60S ribosomal protein L6
    • Protein or peptide: Ribosomal Protein uL30
    • Protein or peptide: 60S ribosomal protein L7a
    • Protein or peptide: 60S ribosomal protein L9
    • Protein or peptide: 60S ribosomal protein L10
    • Protein or peptide: 60S ribosomal protein L11
    • Protein or peptide: Glucose-6-phosphate isomerase
    • Protein or peptide: 60S ribosomal protein L13
    • Protein or peptide: 60S ribosomal protein L14
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: 60S ribosomal protein L13a
    • Protein or peptide: uL22
    • Protein or peptide: eL18
    • Protein or peptide: Ribosomal protein L19
    • Protein or peptide: 60S ribosomal protein L18a
    • Protein or peptide: 60S ribosomal protein L21
    • Protein or peptide: 60S ribosomal protein L22
    • Protein or peptide: Ribosomal protein L23
    • Protein or peptide: Ribosomal protein L24
    • Protein or peptide: Ribosomal_L23eN domain-containing protein
    • Protein or peptide: Ribosomal protein L26
    • Protein or peptide: 60S ribosomal protein L27
    • Protein or peptide: 60S ribosomal protein L27a
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: 60S ribosomal protein L30
    • Protein or peptide: 60S ribosomal protein L31
    • Protein or peptide: Ribosomal protein L32
    • Protein or peptide: 60S ribosomal protein L35a
    • Protein or peptide: 60S ribosomal protein L34
    • Protein or peptide: 60S ribosomal protein L35
    • Protein or peptide: 60S ribosomal protein L36
    • Protein or peptide: Ribosomal protein L37
    • Protein or peptide: 60S ribosomal protein L38
    • Protein or peptide: 60S ribosomal protein L39-like
    • Protein or peptide: Ubiquitin-60S ribosomal protein L40
    • Protein or peptide: eL42
    • Protein or peptide: 60S ribosomal protein L37a
    • Protein or peptide: 60S ribosomal protein L28
    • Protein or peptide: 60S acidic ribosomal protein P0
    • Protein or peptide: 60S ribosomal protein L12
    • Protein or peptide: Ribosomal protein uL1
    • RNA: 18s rRNA
    • Protein or peptide: 40S ribosomal protein S27
    • Protein or peptide: 40S ribosomal protein S28
    • Protein or peptide: Ribosomal protein S27a
    • Protein or peptide: 40S ribosomal protein S30
    • Protein or peptide: eS26
    • Protein or peptide: RACK1
    • Protein or peptide: 40S ribosomal protein S29
    • RNA: E-site tRNA
    • RNA: P-site Val-tRNA
    • Protein or peptide: 40S ribosomal protein SA
    • Protein or peptide: 40S ribosomal protein S3a
    • Protein or peptide: 40S ribosomal protein S2
    • Protein or peptide: 40S ribosomal protein S3
    • Protein or peptide: 40S ribosomal protein S4
    • Protein or peptide: Ribosomal protein S5
    • Protein or peptide: 40S ribosomal protein S6
    • Protein or peptide: 40S ribosomal protein S7
    • Protein or peptide: 40S ribosomal protein S8
    • Protein or peptide: 40S ribosomal protein S9
    • Protein or peptide: S10_plectin domain-containing protein
    • Protein or peptide: 40S ribosomal protein S11
    • Protein or peptide: 40S ribosomal protein S12
    • Protein or peptide: 40S ribosomal protein S13
    • Protein or peptide: 40S ribosomal protein S14
    • Protein or peptide: 40S ribosomal protein uS19
    • Protein or peptide: uS9
    • Protein or peptide: 40S ribosomal protein eS17
    • Protein or peptide: 40S ribosomal protein S18
    • Protein or peptide: 40S ribosomal protein S19
    • Protein or peptide: 40S ribosomal protein uS10
    • Protein or peptide: 40S ribosomal protein S21
    • Protein or peptide: Ribosomal protein S15a
    • Protein or peptide: 40S ribosomal protein S23
    • Protein or peptide: 40S ribosomal protein S24
    • Protein or peptide: 40S ribosomal protein S25
    • Protein or peptide: 60S ribosomal protein L41
• Ligand: ANY 5'-MONOPHOSPHATE NUCLEOTIDE
• Ligand: SPERMIDINE
• Ligand: SPERMINE
• Ligand: MAGNESIUM ION
• Ligand: UNKNOWN ATOM OR ION
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: ACETYLAMINO-ACETIC ACID
• Ligand: ZINC ION
• Ligand: water

Supramolecule #1: Ternary complex of translating ribosome, NAC and METAP1

• Supramolecule: Name: Ternary complex of translating ribosome, NAC and METAP1; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#88
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)

Supramolecule #2: complex of NAC and METAP1 at the tennel exit of translating ribosome

• Supramolecule: Name: complex of NAC and METAP1 at the tennel exit of translating ribosome; type: complex / ID: 2 / Parent: 1 / Macromolecule list: #49-#51

Supramolecule #3: Ribosome translating a cytosolic peptide

• Supramolecule: Name: Ribosome translating a cytosolic peptide / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #1-#48, #52-#88
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: 28S rRNA

• Macromolecule: Name: 28S rRNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 1.5575195 MDa

Sequence

String:

CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGUGGG GCGCGGGAAA UGUGGCGUAC G GAAGACCC ACUCCCCGGC GCCGCUCGUG GGGGGCCCAA GUCCUUCUGA UCGAGGCCCA GCCCGUGGAC GGUGUGAGGC CG GUAGCGG CCCCCGGCGC GCCGGGCCCG GGUCUUCCCG GAGUCGGGUU GCUUGGGAAU GCAGCCCAAA GCGGGUGGUA AAC UCCAUC UAAGGCUAAA UACCGGCACG AGACCGAUAG UCAACAAGUA CCGUAAGGGA AAGUUGAAAA GAACUUUGAA G (A2M)G(A2M)GAGUU CAAGAGGGCG UGAAACCGUU AAGAGGUAAA CGGGUGGGGU CCGCGCAGUC CGCCCGGAGG AUUC AACCC GGCGGCGUGG UCCGGCCGUG CCGGCGGUCU CGGCGGAUCU UUCCCGCUCC CCGUUCCUCC CGACCCCUCC ACCCG CUCC CCCUCCCCCG CCGGUCUUCU CCCUUCACGG GGGGUGGGUC GGCGGGUGCG GGGGUGGGCG GGCGGGGCCG GGGGUG GGG UCGGCGGGGG ACCGCCCCCC GGCCGGCGAC CGGCCGCCGC CGGGCGCAUU UCCACCGCGG CGGUGCGCCG CGACCGG CU CCGGGACGGC UGGGAAGGCC CCGGCGGGGA AGGUGGCCCG GGGGGACGCC UCGCCGCCGU CGUCCGCGGC GGCGGCGG G CGCGACCCCU CCCCGGGUGU UACAGCCCCG CCCCGGCAGC AGCAGCUCGC CGAAUCCCGG GGCCGAGGGA GCGAGACCU GUGUCGCCGC GCUCUCCCCC CUCCCGGCGC UCCCCCCCGC GGGGGUCCCC CGCGAGGGGG CUCCCCCCGC GGGGGCGCGC CGGUGUCGC CGCCCUCGCG GGCGCGGCAC GGGGGGGCCG GGCCGCCCCU CCCACGGCGC GACCGCUCUC CCGGCCCCCG C CGCCGCCU CUUCGGGCGU GCGGCGGGGG UGGGGCGGAC UGUCCCCAGU GCGCCCCGGG CGUGUCACGC CGUCGGGCUC GG GGGAGAA GUCGGCCACG GCGCGUCGCG CGCCUCCGCG UCCCCCCCUC GCCGUCUCCG GGCGGCGGGG GGGGGUUUCG GCG CGGUGU UCUCGGCGCG CGCGCGCGAG CGAGCGCACG GGGUCGGCGG CGAUGUCGGC UACCCACCCG ACCC(OMG)UCUUG (1MA)AAC(A2M)CGGA CCAAGGAGU(OMC) UAACACGUGC GCGAGUCGGG GGCUCGCACG AAAGCCGCCG UGGCGCAAU GAAGGUGAAG GCCGGCGGCG GCUUCCCGCC GUCGUCGGCC GAGGUGGGAU CCCGAGGCCC UUCCAGUCCG CCGAGGGCGC ACCACCGGC CCGUCUCGCC CGCCGCGCCG GGGAGGUGGA GCACGAGCGC ACGUGUUAGG ACCC(OMG)A(A2M)AGA UGG UGA(A2M)C(PSU) AUGCCUGGGC AGGGCGAAGC CAGAGGAAAC UCUGGUGGAG GUCCG(PSU)AGCG GUCCUGACGU GC AAAUCGG UCGUCCGACC UGGGUAUAG(OMG) GGCGAAAGAC UAAUCGAACC AUCUAGUAGC UGGUUCCCUC CGAAGUUUC CC(PSU)CAGGA(PSU)A GCUGGCGCUC UCGCAACCCC GCAGUUUUAU CCGGUAAAGC GAA(PSU)GAUUAG AGGUCUUG G GGCCGAAACG AUCUCAACC(PSU) A(PSU)(PSU)CUCAAAC UU(PSU)AAAUGGG UAAGAAGCCC GGCUCGCUGG CGU GGAGCC GGGCGUGGAA UGCGAGUGCC UAGUGGGCCA C(PSU)U(PSU)UGGUAA GC(A2M)GAACUGG CG(OMC)UGCGG G AUGAACCGAA CGCCGGGUUA AGGCGCCCGA UGCCGACGCU CAUCAGACCC CAGAAAAGGU GUUGGUUGAU AUAGACAGC AGGACGGUGG CCAUGGAAGU CGGAAUCCGC UAAGGAGUGU GUAACAACUC ACCUGCCGAA UCAACUAGCC CUGAAAAUGG AUGGCGCUG GAGCGUCGGG CCCAUACCCG GCCGUCGCUG GCAGUCGGCA GAGCGAGAAA GAGAGUGGAG CGCGCCGCGG G ACUCCCCC CGCGGGGGUC CCCGGCCACC CCCCCCCCGC GGACGCUACG CCGCGACGAG UAGGAGGGCC GCUGCGGUGA GC CUUGAAG CCUAGGGCGC GGGCCCGGGU GGAGCCGCCG CAGGUGCAGA U(OMC)UUGGUGGU AGU(A2M)(OMG)(OMC)A AA UAUUCAAACG AGAACUUUGA AGGCCGAAGU GG(A2M)GAAGGGU UCCAUG(OMU)GAA CAG(OMC)A(OMG)UUGA AC AUGGGUC AGUCGGUCCU GAGAGAUGGG CGAGCGCCGU UCCGAAGGGA CGGGCGAUGG CCUCCGUUGC CCUCAGCCGA (PSU)CGAAAGGG AGUCGGGUUC AGAUCCCCGA AUCCGGAGUG GCGGAGAUGG GCGCCGCGAG GCGUCCAGUG CGGUAAC GC GACCGAUCCC GGAGAAGCCG GCGGGAGCCC CGGGGAGAGU UCUCUU(PSU)UCU UUGUGAAGGG CAGGGCGCCC UGGA AUGGG UUCGCCCCGA GAGAGGGGCC CGUGCCUUGG AAAGCGUCGC GGUUCCGGCG GCGUCCGGUG AGCUCUCGCU GGCCC UUGA AAAUCCGGGG GAGAGGGUGU AAAUCUCGCG CCGGGCCGUA CCC(A2M)UAUCCG CAGCAGGUCU (OMC)CAAGGUG A AC(A2M)GCCUCUG G(OMC)AUGUUGGA ACAA(OMU)GUAGG UAAGGGAAGU CGGCAAGC(OMC)G GAUCCGUAAC UUC (OMG)GGAUA AGGAUUGGCU CUAAGGGCUG GGUCGGUCGG GCUGGGGCGC GAAGCGGGGC UGGGCGCGCG CCGCGGCUGG ACGAGGCGC CGCCGCCCCC CCCACGCCCG GGGCGCCCUC CCCCGGCCGG GUCCGCUCCC UCGCGCGCGC GUGCGCCGUG G CGCGCGCC GCGCGCGCGG CCGAGCCCCG UCUCCCUCCC CCCCUCUCCC CUCCCGGGGC GGGGUGCGGG GAGGGGCGGG CG GGGCCGU CGCUCGCCGC GCGUGCGCGU CGCUGCGCGU GCGCCGCGCG GGGGGUCCGG CGGGCCUCCG GACGGGGGCU CCG GGCACC CGGGCACCCG GGGGGCCGGC GGCGGCGGCG ACUCUGGACG CGAGCCGGGC CCUUCCCGUG GAUCGCCCCA GCUG CGGCG GGCGUCGCGG CCGCCCUCGG GGAGCCCGGC GGGCGCCGCG UGCGCGCGCG UGCGCGCCCG GGGUCGGGGG GGUGC GAGG CAGGGGAGGC CCUCUCUCCU CCUCCCCCAC CCCGGCGCCG CGGCGCCGCG CGUGCGCGCG CGGUCUCCCC CCGCCG GGU CCGCCCCCGG GGCCGCGGUU CCGCGCGGCG CCUCGCCUCG GCCGGCGCCU AGCAGCCGAC UUAGAACUGG UGCGGAC CA GG(OMG)GAAUCCG AC(PSU)G(PSU)UUAAU UAAAACAAAG CAUCGCGAAG GCCCGCGGCG GGUGUUGACG CGAUGU GAU U(PSU)CUGCC(OMC)AG UGCUCUGAAU G(PSU)CA(A2M)AGUGA (A2M)GAAAU(PSU)CAA (PSU)GAAGCGCG G(OMG)UAAACGGC GGGAG(PSU)A(A2M)C(PSU) A(PSU)GAC(PSU)C(PSU)CU UAAGGUAGC(5MC) AA(A2M)U GCCUC (OMG)UCAUCUAAU UAGUGA(OMC)GCG CAUGAA(UY1)GGA (PSU)GA(A2M)CGAG(A2M)U UCCCACUGU (OMC)CC(PSU)ACCUAC (PSU)A(PSU)CCAGCGA AACCAC(A2M)G(OMC)C AAGGGAACGG GCU(PSU)GG(OMC)G G AAUCAGCGG(OMG) GAAAGAAGAC CCUGUUGAGC (PSU)UGAC(OMU)CUAG UCUGGCACGG UGAA(OMG)AGACA UGA GAGGUG UAGAAUAAGU GGGAGGCCCC CGGCGCCCUC CCGCCCCCGC GAGGGGGCGG GGCGGGGUCC GCCGGCCUUG CGGG CCGCC GGUGAAAUAC CACUACUCUU AUCGUUUUUU CACUGACCCG GUGAGGCGGG GGGGCGAGCC CCGAGGGGCU CUCGC UUCU GGCGCCAAGC GCCGGGCCCC GUCGGUCCGC GCGGGCCGGC GGCGGCCGGG CGCGACCCGC UCCGGGGACA GUGCCA GGU GGGGAGUUUG ACUG(OMG)GGCGG UACACCUGUC AAACGGUA(6MZ)C GCAGG(OMU)(OMG)UCC UAAGGCGAGC U CAGGGAGG ACAGAAACCU CCCGUGGAGC AGAAGGGCAA AAGCUCGCUU GA(PSU)CU(PSU)GA(PSU)U UUCAG(OMU) ACG AA(PSU)ACAGACC GUGAAAGCGG GGCCUCACGA UCCUUCUGAG CUU(PSU)UGGGUU U(PSU)AAGCAGGA (OMG) GUGUCAGA AAAGUUACCA CAG(OMG)GAUAAC UGGC(PSU)UGUGG CGGCCAAGCG U(PSU)CA(PSU)AGCGA CG (PSU)CGCUUU UUGA(PSU)CCUU(5MC) GAUGUCGG(OMC)(PSU) CUUCCUAUCA UUG(PSU)GAAGCA GAAUUCACC AAGCGUU(OMG)GA U(OMU)(OMG)(PSU)UCACCC ACUAAUAGGG AACG(PSU)G(A2M)GCU GGG(UR3)U(PSU)AG A (OMC)CGUCGUGAG ACAGGU(PSU)AGU UUUACCCUAC UGAUG(A2M)UGUG (PSU)UG(PSU)UGCCAU GGUA(A2M) UCCU GCUCAGUACG AGAGGAACCG CAG(OMG)U(OMU)CA(OMG)A CAU(PSU)UGGUGU A(PSU)(OMG)UGCUUGG CU GAGGAGC CAAUGGGGCG AAGCUACCA(PSU) CUGUGGGAUU AUGAC(PSU)GAAC GCCUCUAAGU CAGAAUCCCG CCCAG GCGG AACGAUACGG CAGCGCCGAA GGAGCCUCGG UUGGCCUCGG AUAGCCGGUC CCCCGCCGUC CCCGCCGGCG GCCCGC GUC GUGCGUCCGC CUCGGCGGCG UGCGGCGCGC CCCCCGCCGC GCGUCGGGAC CGGGGUCCGG UGCGGAGAGC CCUUCGU CC CGGGAAAUGG GGCGCGGCCG GAAAGGGGGC CGCCCUCUCG CCCGUCACGC ACCGCACGUU CGUGGGGAAC CUGGCGCU A AACCA(PSU)UCGU AGACGACCUG CUUCUGGGUC GGGG(PSU)UUCGU ACG(PSU)AGCAGA GCAGCUCCCU CGCUGCG AU CUAUUGAAAG UCAGCCCUCG ACACAAGGGU UUGU

Macromolecule #2: 5S rRNA

• Macromolecule: Name: 5S rRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 38.691914 KDa

Sequence

String:

GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU U

GENBANK: GENBANK: 4CXE_4

Macromolecule #3: 5.8S rRNA

• Macromolecule: Name: 5.8S rRNA / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 50.809047 KDa

Sequence

String:

CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGC(PSU)GCGAG AAUUAAUG(PSU)G AA UU(OMG)CAGG ACACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGA GCGUCGCUUG

GENBANK: GENBANK: GBCN01009604.1

Macromolecule #52: 18s rRNA

• Macromolecule: Name: 18s rRNA / type: rna / ID: 52 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 603.92825 KDa

Sequence

String:

UACCUGGUUG AUCCUGCCAG UAGCAU(A2M)UGC UUG(PSU)C(PSU)CAAA GAUUAAGCCA UGCAUGUCUA AGUACGCA C GGCCGGUACA GUGAAACUGC GAA(PSU)GGCUC(A2M) UUAAA(PSU)CAG(PSU) UAUGGU(OMU)CC(PSU) U (OMU)GGUCGCU CGCUCCUCUC CUACUUGGAU AACUGUGGUA (A2M)UUCUAG(A2M)GC UAA(OMU)A(OMC)AUGC CG ACGGGCG CUGACCCCCU UCGCGGGGGG GA(PSU)GCGUGCA (PSU)UUAUCAGAU CAAAACCAAC CCGGUCAGCC UCCCC CCGG CCCCGGCCGG GGGGGUGGGC GCCGGCGGCU UUGGUGACUC UAGAUAACCU CGGGCCGAUC GCACGCCCUC CGUGGC GGC GACGACCCAU UCGAACGUC(OMU) GCCCUAUCAA CUUUCGAUGG UAGUCGCCGU GCCUACCAUG GUGACCACGG G (PSU)GACGGGG AAUCAGGGUU CGAU(OMU)CCGGA GA(OMG)GGAGCCU GAGAAACGGC UACCACAU(OMC)C AAGG (A2M)AGGC AGCAGGCGCG C(A2M)AAUUACCC ACUCCCGACC CGGGGA(OMG)GU(A2M) GUGA(OMC)GAAAA AUAAC AAUA CAGGACUCUU UCGAGGCCCU GUAAUUGGAA UGAGUCCACU (PSU)UAA(A2M)UCCUU UAACGAGG(A2M)U CCAU UGGAG (OMG)GCAAGUC(PSU)G GUGCCAGCAG CCGCGG(OMU)AAU UCCAGCUCCA AUA(OMG)CGUA(PSU)A (PSU) UAAAGUUG CUGCAGUU(A2M)A AAAGCUCGUA G(PSU)U(OMG)GA(PSU)CUU GGGAGCGGGC GGGCGGUCCG CCGCGA GGC GAGCCACCGC CCGUCCCCGC CCCUUGCCUC UCGGCGCCCC CUCGAUGCUC UUAGCUGAGU GUCCCGCGGG GCCCGAA GC GUU(PSU)ACUUUG AAAAAA(PSU)(PSU)AG AGUG(PSU)UCAAA GCAGGCCCGA GCCGCCUGGA UACCGCAGCU AG GAA(PSU)AA(PSU) (OMG)GAAUAGGAC CGCGGUUCUA UUUUGUUGGU UUUCGGAACU GAGGCCAUGA UUAAGAGGGA C GGCCGGGG GCAUUCGUAU UGCGCCGCUA GAGGUGAAAU (PSU)CUUGGACCG GCGCAAGACG GACCAGAGCG AAAGCAUU (PSU) GCCAAGAAUG UUUUCAUUAA UCAAGA(A2M)CGA AAGUCGGAGG (PSU)(PSU)CGAAGACG A(PSU)CAGAUAC CGUCGUAGUU CCGACCA(PSU)AA ACGAUGCCGA CUGGCGAUGC GGCGGCGUUA UUCCCAUGAC CCGCCGGGCA GCUUCC GGG AAACCAAAGU CUUUGGGUUC CGGGGGGAGU A(PSU)GG(PSU)UGCAA AGCUGAAACU UAAAGGAAUU GACGGAAGG GCACCACCAG GAGUGGAGCC (PSU)GCGGC(PSU)UAA UU(PSU)GAC(B8N)CAA CACGGGAAAC CUCACCCGGC CCGG ACACG GACAGGA(OMU)UG ACAGAUUGAU AGCUCUUUCU CGAUUCCGUG GGUGG(OMU)G(OMG)UG CAUGGC(4AC)GU UCUUAGU(PSU)GG UGGAGCGAUU UGUCUGG(PSU)UA AUUCCGAUAA CGA(A2M)CGAGAC U(OMC)UGGCAUGC UAAC UAGUU ACGCGACCCC CGAGCGGUCG GCGUCCCCCA ACU(OMU)CU(PSU)A(OMG)A GGGACAAGUG GCGUUCAGCC ACC CGAGAU UGAGCAAUAA CA(OMG)GUCUGUG AUGCCCUUAG AUGUCCGGGG CUGCACGCGC GCUACACUGA CUGGCUCAGC GUGUGCCUA CCCUACGCCG GCAGGCGCGG GUAACCCGUU GAACCCCAUU CGUGAUGGGG AUCGGGGAUU GCAAUUAU (PSU) CCCCAUGAAC GAG(G7M)AAU(PSU)CC CAGUAAGUGC GGGUCAUAAG CUUGCGUUGA UU(A2M)AGUCCCU GCC CUU(PSU)GU ACACACCG(OMC)C CGUCGCUACU ACCGAUUGGA UGGUUUAGUG AGGCCCUCGG AUCGGCCCCG CCGGGG UCG GCCCACGGCC CUGGCGGAGC GCUGAGAAGA CGGUCGAACU (OMU)GACUAUCUA GAGGAAGUAA AAGUCGUA(6MZ) CAAGGUUUC(4AC) GUAGGUG(MA6)(MA6)C CUGCGGAAGG AUCAUUA

GENBANK: GENBANK: GBCT01000564.1

Macromolecule #60: E-site tRNA

• Macromolecule: Name: E-site tRNA / type: rna / ID: 60 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.209544 KDa

Sequence

String:

(N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N) (N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N) (N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)CCA

Macromolecule #61: P-site Val-tRNA

• Macromolecule: Name: P-site Val-tRNA / type: rna / ID: 61 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.451561 KDa

Sequence

String:

GUUUCCGUAG UGUAGUGGUU AUCACGUUCG CCUAACACGC GAAAGGUCCC CGG(5MU)(PSU)CGAAA CCGGGCGGAA AC ACCA

Macromolecule #4: 60S ribosomal protein L8

• Macromolecule: Name: 60S ribosomal protein L8 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 28.103855 KDa

Sequence

String:

MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVY CGKKAQLNIG NVLPVGTMPE GTIVCCLEEK PGDRGKLARA SGNYATVISH NPETKKTRVK LPSGSKKVIS S ANRAVVGV VAGGGRIDKP ILKAGRAYHK YKAKRNCWPR VRGVAMNPVE HPFGGGN(V5N)QH IGKPSTIRRD APAGRKVG L IAARRTGRLR GTKTVQEKEN

UniProtKB: Large ribosomal subunit protein uL2

Macromolecule #5: Ribosomal protein L3

• Macromolecule: Name: Ribosomal protein L3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 46.120996 KDa

Sequence

String:

MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD DAGKRQLDKD FSSMKKYCQV I RVLAHTQM RLLPLRQKKA HLMEIQVNGG TVAEKLDWAR ERLEQQVPVS QVFGQDEMID VIGVTKGKGY KGVTSRWHTK KL PRKT(HIC)RG LRKVACIGAW HPARVAFSVA RAGQKGYHHR TEINKKIYKI GQGYLIKDGK LIKNNASTDY DLSDKSINP LGGFVHYGEV TNDFVMLKGC VVGTKKRVLT LRKSLLVQTK RRALEKIDLK FIDTTSKFGH GRFQTVEEKK AFMGPLKKDR IAKEEGA

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #6: 60S ribosomal protein L4

• Macromolecule: Name: 60S ribosomal protein L4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 46.430895 KDa

Sequence

String:

M(AYA)CARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARI PRV RGGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVED KV EGYKKTKEAV LLLKKLKAWN DIKKVYASQR MRAGKGKMRN RRRIQRRGPC VIYNEDNGIV KAFRNIPGIT LLNVTKLN I LKLAPGGHVG RFCIWTESAF RKLDDLYGTW RKAASLKSNY NLPMHKMLNT DLSRILKSPE IQRALRAPRK KIHRRVLKK NPLKNLRIML KLNPYAKTMR RNTILRQARN HKLRVERAAA ALAAKSDPKE APAKKKPVVG KKKKPVVGRK AAAAKKPAAD KKAADKRAG PEDKKPAA

UniProtKB: Large ribosomal subunit protein uL4

Macromolecule #7: Ribosomal protein L5 eukaryotic C-terminal domain-containing protein

• Macromolecule: Name: Ribosomal protein L5 eukaryotic C-terminal domain-containing protein; type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 34.509879 KDa

Sequence

String:

M(UNK)FVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVC AAY AHELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVTGDE YNVESIDGQP GAFTCYLDAG LARTTTG NK VFGALKGAVD GGLSIPHSTK RFPGYDSESK EFNAEVHRKH IMGQNVADYM RYLMEEDEDA YKKQFSQYIK NNVTPDMM E EMYKKAHAAI RENPVYEKKP KREVKKKRWN RPKMSLAQKK DRVAQKKASF LRAQERAAES

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #8: 60S ribosomal protein L6

• Macromolecule: Name: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 33.028336 KDa

Sequence

String:

MAGEKAPAAK PDATKKSPAK KADHARGKAK KKTLAEKKPK KGKPHCSRNP VLVRGIGRYS RSAMYSRKAL YKRKYAAPKS RIERKKKRE KVLATVTKPV GGDKNGGTRV VKLRKMPRYY PTEDVPRKLL SHGKKPFSQH VRKLRASITP GTILIILTGR H RGKRVVFL KQLSSGLLLV TGPLSLNRVP LRRTHQKFVI ATSTKIDISG VKIPKHLTDA YFKKKKLRKP RHQEGEIFDT EK EKYEITE QRKVDQKAVD SQILPKIKAV PQLQGYLRSV FALTNGVYPH KLVF

UniProtKB: Large ribosomal subunit protein eL6

Macromolecule #9: Ribosomal Protein uL30

• Macromolecule: Name: Ribosomal Protein uL30 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 29.201836 KDa

Sequence

String:

MEGAEEKKKV PAVPETLKKK RRNFAELKIK RLRKKFAQKM LRKARRKLIY EKAKHYHKEY RQMYRTEIRM ARMARKAGNF YVPAEPKLA FVIRIRGING VSPKVRKVLQ LLRLRQIFNG TFVKLNKASI NMLRIVEPYI AWGYPNLKSV NELIYKRGYG K INKKRIAL TDNTLIARSL GKYNIICMED LIHEIYTVGK HFKEANNFLW PFKLSSPRGG MKKKTTHFVE GGDAGNREDQ IN RLIRRMN

UniProtKB: Large ribosomal subunit protein uL30

Macromolecule #10: 60S ribosomal protein L7a

• Macromolecule: Name: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 30.061785 KDa

Sequence

String:

MPKGKKAKGK KVAPAPAVVK KQEAKKVVNP LFEKRPKNFG IGQDIQPKRD LTRFVKWPRY IRLQRQRAIL YKRLKVPPAI NQFTQALDR QTATQLLKLA HKYRPETKQE KKQRLLARAE KKAAGKGDVP TKRPPVLRAG VNTVTTLVEN KKAQLVVIAH D VDPIELVV FLPALCRKMG VPYCILKGKA RLGRLVHRKT CTTVAFTQVN SEDKGALAKL VEAIRTNYND RYDEIRRHWG GN VLGPKSV ARIAKLEKAK AKELATKLG

UniProtKB: Large ribosomal subunit protein eL8

Macromolecule #11: 60S ribosomal protein L9

• Macromolecule: Name: 60S ribosomal protein L9 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 21.871418 KDa

Sequence

String:

MKTILSNQTV DIPENVDISL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELVLEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QADE

UniProtKB: Large ribosomal subunit protein uL6

Macromolecule #12: 60S ribosomal protein L10

• Macromolecule: Name: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.643057 KDa

Sequence

String:

MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLCG HMVSDEYEQL SSEALEAARI CANKYMVKSC GKDGFHIRV RLHPFHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV HIGQVIMSIR TKLQNKEHVV EALRRAKFKF P GRQKIHIS KKWGFTKFNA DEFEDMVAEK RLIPDGCGVK YIPNRGPLDK WRALHS

UniProtKB: Large ribosomal subunit protein uL16

Macromolecule #13: 60S ribosomal protein L11

• Macromolecule: Name: 60S ribosomal protein L11 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 20.288465 KDa

Sequence

String:

MAQDQGEKEN PMRELRIRKL CLNICVGESG DRLTRAAKVL EQLTGQTPVF SKARYTVRSF GIRRNEKIAV HCTVRGAKAE EILEKGLKV REYELRKNNF SDTGNFGFGI QEHIDLGIKY DPSIGIYGLD FYVVLGRPGF SIADKKRRTG CIGAKHRISK E EAMRWFQQ KYDGIILPGK

UniProtKB: Large ribosomal subunit protein uL5

Macromolecule #14: Glucose-6-phosphate isomerase

• Macromolecule: Name: Glucose-6-phosphate isomerase / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO / EC number: glucose-6-phosphate isomerase
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 7.967228 KDa
• Recombinant expression: Organism: Oryctolagus cuniculus (rabbit)

Sequence

String:

MAALTRDPQF QKLQQWYREH RSELNLRRLF DANKDRFNHF SLTLNTNHGH ILVDYSKNLM MMMMV

UniProtKB: Glucose-6-phosphate isomerase

Macromolecule #15: 60S ribosomal protein L13

• Macromolecule: Name: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.331723 KDa

Sequence

String:

MAPSRNGMIL KPHFHKDWQR RVATWFNQPA RKIRRRKARQ ARARRIAPRP AAGPIRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLVLFPR KPSAPKKGDS SAEELKLATQ LTGPVMPIRN V FKKEKARV ITEEEKNFKA FASLRMARAN ARLFGIRAKR AKEAAEQDVE KKK

UniProtKB: Large ribosomal subunit protein eL13

Macromolecule #16: 60S ribosomal protein L14

• Macromolecule: Name: 60S ribosomal protein L14 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 23.870549 KDa

Sequence

String:

MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTRVRRQAM PFKCMQLTDF ILKFPHSARQ KYVRKAWEKA DINTKWAAT RWAKKIEARE RKAKMTDFDR YKVMKAKKMR NRIIKNEVKK LQRAALLKAS PKKAPVAKGA VAAAAAAAKV P AKKATAAG KKAAAQKAPA QKAPAQKAAG QKAAQPPKAQ KGQKPPAQKA PAPKASGKKA

UniProtKB: Large ribosomal subunit protein eL14

Macromolecule #17: Ribosomal protein L15

• Macromolecule: Name: Ribosomal protein L15 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.207285 KDa

Sequence

String:

MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG RKSRGLGKGH KFHHTIGGSR RAAWRRRNTL QLHRYR

UniProtKB: Large ribosomal subunit protein eL15

Macromolecule #18: 60S ribosomal protein L13a

• Macromolecule: Name: 60S ribosomal protein L13a / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 23.533299 KDa

Sequence

String:

MAEGQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY RKKKQLMRLR KQAEKNVEKK ISKFTDVLKT HGLLV

UniProtKB: Large ribosomal subunit protein uL13

Macromolecule #19: uL22

• Macromolecule: Name: uL22 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 21.444221 KDa

Sequence

String:

MVRYSLDPEN PTKSCKSRGS NLRVHFKNTR ETAQAIKGMH IRKATKYLKD VTLKKQCVPF RRYNGGVGRC AQAKQWGWTQ GRWPKKSAE FLLHMLKNAE SNAELKGLDV DSLVIEHIQV NKAPKMRRRT YRAHGRINPY MSSPCHIEMI LTEKEQIVPK P EEEVAQKK KISQKKLKKQ KLMARE

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #20: eL18

• Macromolecule: Name: eL18 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 21.699688 KDa

Sequence

String:

MGVDIRHNKD RKVRRKEPKS QDIYLRLLVK LYRFLARRTN STFNQVVLKR LFMSRTNRPP LSLSRMIRKM KLPGRENKTA VVVGTVTDD VRVQEVPKLK VCALRVTSRA RSRILKAGGK ILTFDQLALD SPKGRGTVLL SGPRKGREVY RHFGKAPGTP H SHTKPYVR SKGRKFERAR GRRASRGYKN

UniProtKB: Large ribosomal subunit protein uL15/eL18 domain-containing protein

Macromolecule #21: Ribosomal protein L19

• Macromolecule: Name: Ribosomal protein L19 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 23.535281 KDa

Sequence

String:

MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ARKRREERLQ AKKEEIIKTL SKEEETKK

UniProtKB: Large ribosomal subunit protein eL19

Macromolecule #22: 60S ribosomal protein L18a

• Macromolecule: Name: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 20.749391 KDa

Sequence

String:

MKASGTLREY KVVGRCLPTP KSPAPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQVMKV EEMAAARCRR PAVKQFHDSR IRFPLPHRVL R RQHKPRFS AKRPNTFF

UniProtKB: Large ribosomal subunit protein eL20

Macromolecule #23: 60S ribosomal protein L21

• Macromolecule: Name: 60S ribosomal protein L21 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 18.609988 KDa

Sequence

String:

MTNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIVVNKQV KGKILAKRI NVRIEHIKHS KSRDSFLKRV KENDQKKKEA KEKGTWVQLK RQPAPPREAH FVRTNGKEPE LLEPIPYEFM A

UniProtKB: Large ribosomal subunit protein eL21

Macromolecule #24: 60S ribosomal protein L22

• Macromolecule: Name: 60S ribosomal protein L22 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.756909 KDa

Sequence

String:

MAPVKKLVAK GGKKKKQVLK FTLDCTHPVE DGIMDAANFE QFLQERIKVN GKAGNLGGGA VTIERSKSKI TVTSEVPFSK RYLKYLTKK YLKKNNLRDW LRVVANSKES YELRYFQINQ DEEEEEDED

UniProtKB: Large ribosomal subunit protein eL22

Macromolecule #25: Ribosomal protein L23

• Macromolecule: Name: Ribosomal protein L23 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.892505 KDa

Sequence

String:

MSKRGRGGSS GAKFRISLGL PVGAVINCAD NTGAKNLYII SVKGIKGRLN RLPAAGVGDM VMATVKKGKP ELRKKVHPAV VIRQRKSYR RKDGVFLYFE DNAGVIVNNK GEMKGSAITG PVAKECADLW PRIASNAGSI A

UniProtKB: Large ribosomal subunit protein uL14

Macromolecule #26: Ribosomal protein L24

• Macromolecule: Name: Ribosomal protein L24 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.825111 KDa

Sequence

String:

MKVELCSFSG YKIYPGHGRR YARTDGKVFQ FLNAKCESAF LSKRNPRQIN WTVLYRRKHK KGQSEEIQKK RTRRAVKFQR AITGASLAD IMAKRNQKPE VRKAQREQAI RAAKEAKKAK QASKKTAMAA AKAPTKAAPK QKIVKPVKVS APRVGGKR

UniProtKB: Large ribosomal subunit protein eL24

Macromolecule #27: Ribosomal_L23eN domain-containing protein

• Macromolecule: Name: Ribosomal_L23eN domain-containing protein / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.768246 KDa

Sequence

String:

MAPKAKKEAP APPKVEAKAK ALKAKKAVLK GVHSHKKKKI RTSPTFRRPK TLRLRRQPKY PRKSAPRRNK LDHYAIIKFP LTTESAMKK IEDNNTLVFI VDVKANKHQI KQAVKKLYDI DVAKVNTLIR PDGEKKAYVR LAPDYDALDV ANKIGII

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #28: Ribosomal protein L26

• Macromolecule: Name: Ribosomal protein L26 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.303363 KDa

Sequence

String:

MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQVG KEKGKYKEET IEKMQE

UniProtKB: Large ribosomal subunit protein uL24

Macromolecule #29: 60S ribosomal protein L27

• Macromolecule: Name: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.835831 KDa

Sequence

String:

MGKFMKPGKV VLVLAGRYSG RKAVIVKNID DGTSDRPYSH ALVAGIDRYP RKVTAAMGKK KIAKRSKIKS FVKVYNYNHL MPTRYSVDI PLDKTVVNKD VFRDPALKRK ARREAKVKFE ERYKTGKNKW FFQKLRF

UniProtKB: Large ribosomal subunit protein eL27

Macromolecule #30: 60S ribosomal protein L27a

• Macromolecule: Name: 60S ribosomal protein L27a / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 16.635551 KDa

Sequence

String:

MPSRLRKTRK LRGHVSHGHG RIGKHRKHPG GRGNAGGM(V5N)H HRINFDKYHP GYFGKVGMRH YHLKRNQSFC PTVNLD KLW TLVSEQTRVN AAKNKTGAAP IIDVVRSGYY KVLGKGKLPK QPVIVKAKFF SRRAEEKIKG VGGACVLVA

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #31: 60S ribosomal protein L29

• Macromolecule: Name: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 26.721727 KDa

Sequence

String:

MAKS(MLZ)NHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAMAARAE AIKALV KPK EVKPTIPKGV SRKLHRLAYI AHPKLGRRAR ARIARGLRLS RPQTKAKAKT EPQIKGKVKA QIKAQAQAQI KSKGKGK AQ AETKPKAQAE TKPKAQAQAK PKAQAQGKPK AQAQGKPKAQ AQAKPKAQAQ AKPKAQAQTK PKAQATPAAP VPAQAPPK G AQPPAKAP

UniProtKB: Large ribosomal subunit protein eL29

Macromolecule #32: 60S ribosomal protein L30

• Macromolecule: Name: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 12.807065 KDa

Sequence

String:

MVAAKKTKKS LESINSRLQL VMKSGKYVLG YKQSLKMIRQ GKAKLVILAN NCPALRKSEI EYYAMLAKTG VHHYSGNNIE LGTACGKYY RVCTLSIIDP GDSDIIRSMP EQTGEK

UniProtKB: Large ribosomal subunit protein eL30

Macromolecule #33: 60S ribosomal protein L31

• Macromolecule: Name: 60S ribosomal protein L31 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.494938 KDa

Sequence

String:

MAPAKKGGEK KKGRSAINEV VTREYTINIH KRIHGVGFKK RAPRALKEIR KFAMKEMGTP DVRIDTRLNK AVWAKGIRNV PYRIRVRLS RKRNEDEDSP NKLYTLVTYV PVTTFKNLQT VNVDEN

UniProtKB: Large ribosomal subunit protein eL31

Macromolecule #34: Ribosomal protein L32

• Macromolecule: Name: Ribosomal protein L32 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.898932 KDa

Sequence

String:

MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY GSNKKTKHML PSGFRKFLVH NVKELEVLL MCNKSYCAEI AHNVSSKNRK AIVERAAQLA IRVTNPNARL RSEENE

UniProtKB: Large ribosomal subunit protein eL32

Macromolecule #35: 60S ribosomal protein L35a

• Macromolecule: Name: 60S ribosomal protein L35a / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 12.580809 KDa

Sequence

String:

MSGRLWCKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFR SNLPAKAIGH RIRVMLYPSR I

UniProtKB: Large ribosomal subunit protein eL33

Macromolecule #36: 60S ribosomal protein L34

• Macromolecule: Name: 60S ribosomal protein L34 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 13.326074 KDa

Sequence

String:

MVQRLTYRRR LSYNTASNKT RLSRTPGNRI VYLYTKKVGK APKSACGVCP GRLRGVRAVR PKVLMRLSKT KKHVSRAYGG SMCAKCVRD RIKRAFLIEE QKIVVKVLKA QAQSQKAK

UniProtKB: Large ribosomal subunit protein eL34

Macromolecule #37: 60S ribosomal protein L35

• Macromolecule: Name: 60S ribosomal protein L35 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.566599 KDa

Sequence

String:

MAKIKARDLR GKKKEELLKQ LDDLKVELSQ LRVAKVTGGA ASKLSKIRVV RKSIARVLTV INQTQKENLR KFYKGKKYKP LDLRPKKTR AMRRRLNKHE ESLKTKKQQR KERLYPLRKY AVKA

UniProtKB: Large ribosomal subunit protein uL29

Macromolecule #38: 60S ribosomal protein L36

• Macromolecule: Name: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 12.263834 KDa

Sequence

String:

MALRYPMAVG LNKGHKVTKN VSKPRHSRRR GRLTKHTKFV RDMIREVCGF APYERRAMEL LKVSKDKRAL KFIKKRVGTH IRAKRKREE LSSVLAAMRK AAAKKD

UniProtKB: Large ribosomal subunit protein eL36

Macromolecule #39: Ribosomal protein L37

• Macromolecule: Name: Ribosomal protein L37 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 11.111032 KDa

Sequence

String:

MTKGTSSFGK RRNKTHTLCR RCGSKAYHLQ KSTCGKCGYP AKRKRKYNWS AKAKRRNTTG TGRMRHLKIV YRRFRHGFRE GTTPKPKRA AVAASSSS

UniProtKB: Large ribosomal subunit protein eL37

Macromolecule #40: 60S ribosomal protein L38

• Macromolecule: Name: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 8.238948 KDa

Sequence

String:

MPRKIEEIKD FLLTARRKDA KSVKIKKNKD NVKFKVRCSR YLYTLVITDK EKAEKLKQSL PPGLAVKELK

UniProtKB: Large ribosomal subunit protein eL38

Macromolecule #41: 60S ribosomal protein L39-like

• Macromolecule: Name: 60S ribosomal protein L39-like / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 6.455775 KDa

Sequence

String:

MSSHKTFRIK RFLAKKQKQN RPIPQWIWMK TGNKIRYNSK RRHWRRTKLG L

UniProtKB: Large ribosomal subunit protein eL39

Macromolecule #42: Ubiquitin-60S ribosomal protein L40

• Macromolecule: Name: Ubiquitin-60S ribosomal protein L40 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.800474 KDa

Sequence

String:

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGIIEP SLRQLAQKY NCDKMICR(M3L)C YARLHPRAVN CRKKKCGHTN NLRPKKKVK

UniProtKB: Ubiquitin-ribosomal protein eL40 fusion protein

Macromolecule #43: eL42

• Macromolecule: Name: eL42 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 12.489991 KDa

Sequence

String:

MVNVPKTRRT FCKKCGKHQP HKVTQYKKGK DSLYAQGKRR YDRKQSGYGG QT(MLZ)PIFRKKA KTTKKIVLRL ECVEPN CRS KRMLAIKRCK HFELGGDKKR KGQVIQF

UniProtKB: Large ribosomal subunit protein eL42

Macromolecule #44: 60S ribosomal protein L37a

• Macromolecule: Name: 60S ribosomal protein L37a / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 10.29935 KDa

Sequence

String:

MAKRTKKVGI VGKYGTRYGA SLRKMVKKIE ISQHAKYTCS FCGKTKMKRR AVGIWHCGSC MKTVAGGAWT YNTTSAVTVK SAIRRLKEL KDQ

UniProtKB: Large ribosomal subunit protein eL43

Macromolecule #45: 60S ribosomal protein L28

• Macromolecule: Name: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.844696 KDa

Sequence

String:

M(SAC)AHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV VVVMKRRSGQ RKPATS YVR TTINKNARAT LSSIRHMIRK NKYRPDLRMA AIRRASAILR SQKPVMVKRK RTRPTKSS

UniProtKB: Large ribosomal subunit protein eL28

Macromolecule #46: 60S acidic ribosomal protein P0

• Macromolecule: Name: 60S acidic ribosomal protein P0 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 34.380504 KDa

Sequence

String:

MPREDRATWK SNYFLKIIQL LDDYPKCFIV GADNVGSKQM QQIRMSLRGK AVVLMGKNTM MRKAIRGHLE NNPALEKLLP HIRGNVGFV FTKEDLTEIR DMLLANKVPA AARAGAIAPC EVTVPAQNTG LGPEKTSFFQ ALGITTKISR GTIEILSDVQ L IKTGDKVG ASEATLLNML NISPFSFGLI IQQVFDNGSI YNPEVLDITE DTLHSRFLEG VRNVASVCLQ IGYPTVASVP HS IINGYKR VLALSVETEY TFPLAEKVKA FLADPSAFVA AAPVAAASTA APAAAAAAPA KVEAKEESEE SDEDMGFGLF D

UniProtKB: Large ribosomal subunit protein uL10

Macromolecule #47: 60S ribosomal protein L12

• Macromolecule: Name: 60S ribosomal protein L12 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.847619 KDa

Sequence

String:

MPPKFDPNEI KVVYLRCTGG EVGATSALAP KIGPLGLSPK KVGDDIAKAT GDWKGLRITV KLTIQNRQAQ IEVVPSASAL IIKALKEPP RDRKKQKNIK HSGNITFDEI VNIARQMRHR SLARELSGTI KEILGTAQSV GCNVDGRHPH DIIDDINSGA V ECPAS

UniProtKB: Large ribosomal subunit protein uL11

Macromolecule #48: Ribosomal protein uL1

• Macromolecule: Name: Ribosomal protein uL1 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.87541 KDa

Sequence

String:

MGDILSRDTL YEAVREVLHG NQRKRRKFLE TVELQISLKN YDPQKDKRFS GTVRLKSTPR PKFSVCVLGD QQHCDEAKAV DIPHMDIEA LKKLNKNKKL VKKLAKKYDA FLASESLIKQ IPRILGPGLN KAGKFPSLLT HNENMVAKVD EVKSTIKFQM K KVLCLAVA VGHVKMTDDE LVYNIHLAVN FLVSLLKKNW QNVRALYIKS TMGKPQRLY

UniProtKB: Large ribosomal subunit protein uL1

Macromolecule #49: Methionine aminopeptidase 1

• Macromolecule: Name: Methionine aminopeptidase 1 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO / EC number: methionyl aminopeptidase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 43.274195 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MAAVETRVCE TDGCSSEAKL QCPTCIKLGI QGSYFCSQEC FKGSWATHKL LHKKAKDEKA KREVSSWTVE GDINTDPWAG YRYTGKLRP HYPLMPTRPV PSYIQRPDYA DHPLGMSESE QALKGTSQIK LLSSEDIEGM RLVCRLAREV LDVAAGMIKP G VTTEEIDH AVHLACIARN CYPSPLNYYN FPKSCCTSVN EVICHGIPDR RPLQEGDIVN VNITLYRNGY HGDLNETFFV GE VDDGARK LVQTTYECLM QAIDAVKPGV RYRELGNIIQ KHAQANGFSV VRSYCGHGIH KLFHTAPNVP HYAKNKAVGV MKS GHVFTI EPMICEGGWQ DETWPDGWTA VTRDGKRSAQ FEHTLLVTDT GCEILTRRLD SARPHFMSQF

UniProtKB: Methionine aminopeptidase 1

Macromolecule #50: Nascent polypeptide-associated complex subunit alpha

• Macromolecule: Name: Nascent polypeptide-associated complex subunit alpha / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.406824 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MPGEATETVP ATEQELPQPQ AETGSGTESD SDESVPELEE QDSTQATTQQ AQLAAAAEID EEPVSKAKQS RSEKKARKAM SKLGLRQVT GVTRVTIRKS KNILFVITKP DVYKSPASDT YIVFGEAKIE DLSQQAQLAA AEKFKVQGEA VSNIQENTQT P TVQEESEE EEVDETGVEV KDIELVMSQA NVSRAKAVRA LKNNSNDIVN AIMELTM

UniProtKB: Nascent polypeptide-associated complex subunit alpha

Macromolecule #51: Transcription factor BTF3

• Macromolecule: Name: Transcription factor BTF3 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.493821 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MKETIMNQEK LAKLQAQVRI GGKGTARRKK KVVHRTATAD DKKLQFSLKK LGVNNISGIE EVNMFTNQGT VIHFNNPKVQ ASLAANTFT ITGHAETKQL TEMLPSILNQ LGADSLTSLR RLAEALPKQL SGC

UniProtKB: Transcription factor BTF3

Macromolecule #53: 40S ribosomal protein S27

• Macromolecule: Name: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 9.480186 KDa

Sequence

String:

MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH

UniProtKB: Small ribosomal subunit protein eS27

Macromolecule #54: 40S ribosomal protein S28

• Macromolecule: Name: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 7.855052 KDa

Sequence

String:

MDTSRVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV REGDVLTLLE SEREARRLR

UniProtKB: Small ribosomal subunit protein eS28

Macromolecule #55: Ribosomal protein S27a

• Macromolecule: Name: Ribosomal protein S27a / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 18.004041 KDa

Sequence

String:

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGAKKR KKKSYTTPK KNKHKRKKVK LAVLKYYKVD ENGKISRLRR ECPSDECGAG VFMASHFDRH YCGKCCLTYC FNKPEDK

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

Macromolecule #56: 40S ribosomal protein S30

• Macromolecule: Name: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.498884 KDa

Sequence

String:

MQLFVRAQEL HTLEVTGRET VAQIKAHVAS LEGIAPEDQV VLLAGTPLED EATLGQCGVE ALSTLEVAGR MLGGKVHGSL ARVGKVRGQ TLKVAKQEKK KKRTGRAKRR MQYNRRFVNV VPTFGKKKGP NANS

UniProtKB: Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein

Macromolecule #57: eS26

• Macromolecule: Name: eS26 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 13.047532 KDa

Sequence

String:

MTKKRRNNGR AKKGRGHVQP IRCTNCARCV PKDKAIKKFV IRNIVEAAAV RDISEASVFD AYVLPKLYVK LHYCVSCAIH SKVVRNRSR EARKDRTPPP RFRPAGAAPR PPPKPM

UniProtKB: 40S ribosomal protein S26

Macromolecule #58: RACK1

• Macromolecule: Name: RACK1 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 35.115652 KDa

Sequence

String:

MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC GWDKLVKVWN LANCKLKTNH IGHTGYLNTV TVSPDGSLCA SGGKDGQAML WDLNEGKHLY TLDGGDIINA LC FSPNRYW LCAATGPSIK IWDLEGKIIV DELKQEVIST SSKAEPPQCT SLAWSADGQT LFAGYTDNLV RVWQVTIGTR

UniProtKB: Small ribosomal subunit protein RACK1

Macromolecule #59: 40S ribosomal protein S29

• Macromolecule: Name: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 6.690821 KDa

Sequence

String:

MGHQQLYWSH PRKFGQGSRS CRVCSNRHGL IRKYGLNMCR QCFRQYAKDI GFIKLD

UniProtKB: Small ribosomal subunit protein uS14

Macromolecule #62: 40S ribosomal protein SA

• Macromolecule: Name: 40S ribosomal protein SA / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 32.970023 KDa

Sequence

String:

M(SAC)GALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSV ISS RNTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQAAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRY VD IAIPCNNKGA HSVGLMWWML AREVLRMRGT ISREHPWEVM PDLYFYRDPE EIEKEEQAAA EKAVTKEEFQ GEWTAPAP E FTATQPEVAD WSEGVQVPSV PIQQFPTEDW SAQPATEDWS AAPTAQATEW VGTTTEWS

UniProtKB: Small ribosomal subunit protein uS2

Macromolecule #63: 40S ribosomal protein S3a

• Macromolecule: Name: 40S ribosomal protein S3a / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 30.002061 KDa

Sequence

String:

MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM MEIMTREVQT NDLKEVVNKL IPDSIGKDIE KACQSIYPLH DVFVRKVKML KKPKFELGKL MELHGEGSSS GK ATGDETG AKVERADGYE PPVQESV

UniProtKB: Small ribosomal subunit protein eS1

Macromolecule #64: 40S ribosomal protein S2

• Macromolecule: Name: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 31.285426 KDa

Sequence

String:

MADDAGAAGG PGGPGGPGMG GRGGFRGGFG SGIRGRGRGR GRGRGRGRGA RGGKAEDKEW LPVTKLGRLV KDMKIKSLEE IYLFSLPIK ESEIIDFFLG AALKDEVLKI MPVQKQTRAG QRTRFKAFVA IGDYNGHVGL GVKCSKEVAT AIRGAIILAK L SIVPVRRG YWGNKIGKPH TVPCKVTGRC GSVLVRLIPA PRGTGIVSAP VPKKLLMMAG IDDCYTSARG CTATLGNFAK AT FDAISKT YSYLTPDLWK ETVFTKSPYQ EFTDHLVKTH TRVSVQRTQA PAVATT

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #65: 40S ribosomal protein S3

• Macromolecule: Name: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-(apurinic or apyrimidinic site) lyase
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 31.146607 KDa

Sequence

String:

MSARRRRRRA AFRRRAEPFI PISVREPLPF LSAARGGKMA VQISKKRKFV ADGIFKAELN EFLTRELAED GYSGVEVRVT PTRTEIIIL ATRTQNVLGE KGRRIRELTA VVQKRFGFPE GSVELYAEKV ATRGLCAIAQ AESLRYKLLG GLAVRRACYG V LRFIMESG AKGCEVVVSG KLRGQRAKSM KFVDGLMIHS GDPVNYYVDT AVRHVLLRQG VLGIKVKIML PWDPSGKIGP KK PLPDHVS IVEPKDEILP TTPISEQKGG KPEPPAMPQP VPTA

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #66: 40S ribosomal protein S4

• Macromolecule: Name: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 29.654869 KDa

Sequence

String:

MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE VKKICMQRFI KIDGKVRTDI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT I QIDLETGK ITDFIKFDTG NLCMVTGGAN LGRIGVITNR ERHPGSFDVV HVKDANGNSF ATRLSNIFVI GKGNKPWISL PR GKGIRLT IAEERDKRLA AKQSSG

UniProtKB: Small ribosomal subunit protein eS4

Macromolecule #67: Ribosomal protein S5

• Macromolecule: Name: Ribosomal protein S5 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 22.913453 KDa

Sequence

String:

MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT IAECLADELI NAAKGSSNSY AIKKKDELER VAKSNR

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #68: 40S ribosomal protein S6

• Macromolecule: Name: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 28.751906 KDa

Sequence

String:

MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRRRLSSLR AS TSKSESS QK

UniProtKB: Small ribosomal subunit protein eS6

Macromolecule #69: 40S ribosomal protein S7

• Macromolecule: Name: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 47.356172 KDa

Sequence

String:

EPITNRVFQA LTSSDFKQQD GGSFSLSRTH LSGFRSRPRR TTIASAATRP PPIPSRGGSA PTGTPEGAGA SRSSRTVPRL PPQPSGPPR APRHPGFQRA LPSLIRPEPG VRAPLLASAG TRFKRPASQA VKALSARGLL QPQVQVGPRA NRVPQRREPR Q HLSGHAPG ASEGGSASPT YWSLGITSWD ALGFPLRLGG SSGFRSSVAL FRPLSFRRGD LPRSREIWAS SSAGAQPGEA MF SSSAKIV KPNGEKPDEF ESGISQALLE LEMNSDLKAQ LRELNITAAK EIEVGGGRKA IIIFVPVPQL KSFQKIQVRL VRE LEKKFS GKHVVFIAQR RILPKPTRKS RTKNKQKRPR SRTLTAVHDA ILEDLVFPSE IVGKRIRVKL DGSRLIKVHL DKAQ QNNVE HKVETFSGVY KKLTGKDVNF EFPEFQL

UniProtKB: Small ribosomal subunit protein eS7

Macromolecule #70: 40S ribosomal protein S8

• Macromolecule: Name: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.263387 KDa

Sequence

String:

MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ GKLLACIASR PGQCGRADGY VLEGKELEFY LRKIKARKGK

UniProtKB: Small ribosomal subunit protein eS8

Macromolecule #71: 40S ribosomal protein S9

• Macromolecule: Name: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 22.641564 KDa

Sequence

String:

MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG RPGRVKRKNA KKGQGGAGAG DDEEED

UniProtKB: Small ribosomal subunit protein uS4

Macromolecule #72: S10_plectin domain-containing protein

• Macromolecule: Name: S10_plectin domain-containing protein / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 18.933846 KDa

Sequence

String:

MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSRP ETGRPRPKGL EGERPARLTR GEADRDTYRR SAVPPGADKK AEAGAGSATE FQFRGGFGRG R GQPPQ

UniProtKB: Small ribosomal subunit protein eS10

Macromolecule #73: 40S ribosomal protein S11

• Macromolecule: Name: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 18.468826 KDa

Sequence

String:

MADIQTERAY QKQPTIFQNK KRVLLGETGK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #74: 40S ribosomal protein S12

• Macromolecule: Name: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.538987 KDa

Sequence

String:

MAEEGIAAGG VMDVNTALQE VLKTALIHDG LARGIREAAK ALDKRQAHLC VLASNCDEPM YVKLVEALCA EHQINLIKVD DNKKLGEWV GLCKIDREGK PRKVVGCSCV VVKDYGKESQ AKDVIEEYFK CKK

UniProtKB: Small ribosomal subunit protein eS12

Macromolecule #75: 40S ribosomal protein S13

• Macromolecule: Name: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.259389 KDa

Sequence

String:

MGRMHAPGKG LSQSALPYRR SVPTWLKLTS DDVKEQIYKL AKKGLTPSQI GVILRDSHGV AQVRFVTGNK ILRILKSKGL APDLPEDLY HLIKKAVAVR KHLERNRKDK DAKFRLILIE SRIHRLARYY KTKRVLPPNW KYESSTASAL VA

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #76: 40S ribosomal protein S14

• Macromolecule: Name: 40S ribosomal protein S14 / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 16.316798 KDa

Sequence

String:

MAPRKGKEKK EEQVISLGPQ VAEGENVFGV CHIFASFNDT FVHVTDLSGK ETICRVTGGM KVKADRDESS PYAAMLAAQD VAQRCKELG ITALHIKLRA TGGNRTKTPG PGAQSALRAL ARSGMKIGRI EDVTPIPS(5F0)S TRRKGGRRGR RL

UniProtKB: Small ribosomal subunit protein uS11

Macromolecule #77: 40S ribosomal protein uS19

• Macromolecule: Name: 40S ribosomal protein uS19 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.049182 KDa

Sequence

String:

MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLSRGLR RKQHSLLKRL RKAKKEAPPM EKPEVVKTHL RDMIILPEM VGSMVGVYNG KTFNQVEIKP EMIGHYLGEF SITYKPVKHG RPGIGATHSS RFIPLK

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #78: uS9

• Macromolecule: Name: uS9 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 19.213465 KDa

Sequence

String:

SARVAPRFPF PVVAPRGEDR HSSAAAMPSK GPLQSVQVFG RKKTATAVAH CKRGNGLIKV NGRPLEMIEP RTLQYKLLEP VLLLGKERF AGVDIRVRVK GGGHVAQIYA IRQSISKALV AYYQKYVDEA SKKEIKDILI QYDRTLLVAD PRRCESKKFG G PGARARYQ KSYR

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #79: 40S ribosomal protein eS17

• Macromolecule: Name: 40S ribosomal protein eS17 / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.552119 KDa

Sequence

String:

MGRVRTKTVK KAARVIIEKY YTRLGNDFHT NKRVCEEIAI IPSKKLRNKI AGYVTHLMKR IQRGPVRGIS IKLQEEERER RDNYVPEVS ALDQEIIEVD PDTKEMLKLL DFGSLSNLQV TQPTVGMNFK TPRGAV

UniProtKB: Small ribosomal subunit protein eS17

Macromolecule #80: 40S ribosomal protein S18

• Macromolecule: Name: 40S ribosomal protein S18 / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.801814 KDa

Sequence

String:

M(SAC)LVIPEKFQ HILRVLNTNI DGRRKIAFAI TAIKGVGRRY AHVVLRKADI DLTKRAGELT EDEVERVITI MQNPRQ YKI PDWFLNRQKD VKDGKYSQVL ANGLDNKLRE DLERLKKIRA HRGLRHFWGL RVRGQHTKTT GRRGRTVGVS KKK

UniProtKB: Small ribosomal subunit protein uS13

Macromolecule #81: 40S ribosomal protein S19

• Macromolecule: Name: 40S ribosomal protein S19 / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 16.104579 KDa

Sequence

String:

MPGVTVKDVN QQEFVRALAA FLKKSGKLKV PEWVDTVKLA KHKELAPYDE NWFYTRAAST ARHLYL(NMM)GGA GVGSMT KIY GGRQRNGVMP SHFSRGSKSV ARRVLQALEG LKMVEKDQDG GRKLTPQGQR DLDRIAGQVA AANKKH

UniProtKB: Small ribosomal subunit protein eS19

Macromolecule #82: 40S ribosomal protein uS10

• Macromolecule: Name: 40S ribosomal protein uS10 / type: protein_or_peptide / ID: 82 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 13.398763 KDa

Sequence

String:

MAFKDTGKTP VEPEVAIHRI RITLTSRNVK SLEKVCADLI RGAKEKNLKV KGPVRMPTKT LRITTRKTPC GEGSKTWDRF QMRIHKRLI DLHSPSEIVK QITSISIEPG VEVEVTIADA

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #83: 40S ribosomal protein S21

• Macromolecule: Name: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 83 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 9.181374 KDa

Sequence

String:

(AME)QNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKSD GIV SQNF

UniProtKB: Small ribosomal subunit protein eS21

Macromolecule #84: Ribosomal protein S15a

• Macromolecule: Name: Ribosomal protein S15a / type: protein_or_peptide / ID: 84 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.865555 KDa

Sequence

String:

MVRMNVLADA LKSINNAEKR GKRQVLIRPC SKVIVRFLTV MMKHGYIGEF EIIDDHRAGK IVVNLTGRLN KCGVISPRFD VQLKDLEKW QNNLLPSRQF GFIVLTTSAG IMDHEEARRK HTGGKILGFF F

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #85: 40S ribosomal protein S23

• Macromolecule: Name: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 85 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.860666 KDa

Sequence

String:

MGKCRGLRTA RKLRSHRRDQ KWHDKQYKKA HLGTALKANP FGGASHAKGI VLEKVGVEAK Q(HY3)NSAIRKCV RVQLIK NGK KITAFVPNDG CLNFIEENDE VLVAGFGRKG HAVGDIPGVR FKVVKVANVS LLALYKGKKE RPRS

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #86: 40S ribosomal protein S24

• Macromolecule: Name: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 86 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.107924 KDa

Sequence

String:

MNDTVTIRTR KFMTNRLLQR KQMVIDVLHP GKATVPKTEI REKLAKMYKT TPDVIFVFGF RTHFGGGKTT GFGMIYDSLD YAKKNEPKH RLARHGLYEK KKTSRKQRKE RKNRMKKVRG TAKANVGAGK K

UniProtKB: 40S ribosomal protein S24

Macromolecule #87: 40S ribosomal protein S25

• Macromolecule: Name: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 87 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 13.645028 KDa

Sequence

String:

PPKDDKKKKD AGKSAKKDKD PVNKSGGKAK KKKWSKGKVR DKLNNLVLFD KATYDKLCKE VPNYKLITPA VVSERLKIRG SLARAALQE LLSKGLIKLV SKHRAQVIYT RNTKGGDAPA AGEDA

UniProtKB: Small ribosomal subunit protein eS25

Macromolecule #88: 60S ribosomal protein L41

• Macromolecule: Name: 60S ribosomal protein L41 / type: protein_or_peptide / ID: 88 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 3.473451 KDa

Sequence

String:

MRAKWRKKRM RRLKRKRRKM RQRSK

UniProtKB: Small ribosomal subunit protein eS32

Macromolecule #89: ANY 5'-MONOPHOSPHATE NUCLEOTIDE

• Macromolecule: Name: ANY 5'-MONOPHOSPHATE NUCLEOTIDE / type: ligand / ID: 89 / Number of copies: 73 / Formula: N
• Molecular weight: Theoretical: 214.11 Da

Chemical component information

ChemComp-N:
ANY 5'-MONOPHOSPHATE NUCLEOTIDE

Macromolecule #90: SPERMIDINE

• Macromolecule: Name: SPERMIDINE / type: ligand / ID: 90 / Number of copies: 30 / Formula: SPD
• Molecular weight: Theoretical: 145.246 Da

Chemical component information

ChemComp-SPD:
SPERMIDINE

Macromolecule #91: SPERMINE

• Macromolecule: Name: SPERMINE / type: ligand / ID: 91 / Number of copies: 3 / Formula: SPM
• Molecular weight: Theoretical: 202.34 Da

Chemical component information

ChemComp-SPM:
SPERMINE

Macromolecule #92: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 92 / Number of copies: 420 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #93: UNKNOWN ATOM OR ION

• Macromolecule: Name: UNKNOWN ATOM OR ION / type: ligand / ID: 93 / Number of copies: 312 / Formula: UNX

Macromolecule #94: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 94 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #95: ACETYLAMINO-ACETIC ACID

• Macromolecule: Name: ACETYLAMINO-ACETIC ACID / type: ligand / ID: 95 / Number of copies: 1 / Formula: AAC
• Molecular weight: Theoretical: 117.103 Da

Chemical component information

ChemComp-AAC:
ACETYLAMINO-ACETIC ACID

Macromolecule #96: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 96 / Number of copies: 8 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #97: water

• Macromolecule: Name: water / type: ligand / ID: 97 / Number of copies: 2117 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.6
• Grid: Model: Quantifoil R2/2 / Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 15 sec.
• Vitrification: Cryogen name: ETHANE-PROPANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.4 µm / Nominal defocus min: 0.6 µm / Nominal magnification: 81000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

7o7y

Details: 2B3K for METAP1; 7QWR for NAC; 7O7Y for ribosome

• Final reconstruction: Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 21221
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD

Atomic model buiding 1

Initial model

PDB ID	Chain
7o7y	source_name: PDB, initial_model_type: experimental model
2b3k	source_name: PDB, initial_model_type: experimental model
7qwr	source_name: PDB, initial_model_type: experimental model

• Refinement: Space: REAL / Protocol: RIGID BODY FIT

Output model

PDB-8p2k:
Ternary complex of translating ribosome, NAC and METAP1