EMDB-17297: Structure of a human 48S translation initiation complex with eIF4...

Basic information

Entry

Database: EMDB / ID: EMD-17297

• Title: Structure of a human 48S translation initiation complex with eIF4F and eIF4A
• Map data: Human 48S complex

Sample

• Complex: Human 48S translation initiation complex
  • Protein or peptide: x 53 types
  • RNA: x 3 types
• Ligand: x 2 types

• Keywords: translation / eIF4A / eIF4F / initiation / ribosome / mRNA

Function / homology

Function:

positive regulation of eukaryotic translation initiation factor 4F complex assembly / male germ cell proliferation / eukaryotic initiation factor eIF2 binding / positive regulation of translation in response to endoplasmic reticulum stress / translation initiation ternary complex / regulation of translation in response to endoplasmic reticulum stress / glial limiting end-foot / HRI-mediated signaling / response to manganese-induced endoplasmic reticulum stress / viral translational termination-reinitiation / Cellular response to mitochondrial stress / positive regulation of type B pancreatic cell apoptotic process / Response of EIF2AK1 (HRI) to heme deficiency / Recycling of eIF2:GDP / methionyl-initiator methionine tRNA binding / Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / negative regulation of translational initiation in response to stress / eukaryotic translation initiation factor 3 complex, eIF3e / cap-dependent translational initiation / eukaryotic initiation factor 4E binding / PERK-mediated unfolded protein response / eukaryotic translation initiation factor 3 complex, eIF3m / PERK regulates gene expression / response to kainic acid / IRES-dependent viral translational initiation / translation reinitiation / eukaryotic translation initiation factor 2 complex / RNA cap binding / eukaryotic translation initiation factor 4F complex / nuclear stress granule / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / formation of cytoplasmic translation initiation complex / multi-eIF complex / regulation of translational initiation in response to stress / regulation of cellular response to stress / eukaryotic translation initiation factor 3 complex / eukaryotic 43S preinitiation complex / translation factor activity, RNA binding / Deadenylation of mRNA / mRNA cap binding / formation of translation preinitiation complex / miRNA-mediated gene silencing by inhibition of translation / eukaryotic 48S preinitiation complex / M-decay: degradation of maternal mRNAs by maternally stored factors / negative regulation of endoplasmic reticulum unfolded protein response / oxidized pyrimidine DNA binding / response to TNF agonist / positive regulation of base-excision repair / positive regulation of ubiquitin-protein transferase activity / regulation of translational initiation / positive regulation of respiratory burst involved in inflammatory response / positive regulation of gastrulation / protein-synthesizing GTPase / protein tyrosine kinase inhibitor activity / positive regulation of DNA-templated transcription initiation / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage / IRE1-RACK1-PP2A complex / positive regulation of Golgi to plasma membrane protein transport / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / nucleolus organization / TNFR1-mediated ceramide production / GDP-dissociation inhibitor activity / negative regulation of RNA splicing / positive regulation of protein localization to cell periphery / neural crest cell differentiation / metal-dependent deubiquitinase activity / supercoiled DNA binding / cytoplasmic translational initiation / NF-kappaB complex / negative regulation of DNA repair / oxidized purine DNA binding / cysteine-type endopeptidase activator activity involved in apoptotic process / rRNA modification in the nucleus and cytosol / negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide / negative regulation of bicellular tight junction assembly / ubiquitin-like protein conjugating enzyme binding / regulation of establishment of cell polarity / negative regulation of phagocytosis / erythrocyte homeostasis / cytoplasmic side of rough endoplasmic reticulum membrane / Formation of the ternary complex, and subsequently, the 43S complex / ion channel inhibitor activity / protein kinase A binding / laminin receptor activity / Ribosomal scanning and start codon recognition / pigmentation / positive regulation of mitochondrial depolarization / Translation initiation complex formation / positive regulation of protein metabolic process / negative regulation of Wnt signaling pathway / fibroblast growth factor binding / Protein hydroxylation / monocyte chemotaxis / BH3 domain binding / negative regulation of translational frameshifting / regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway / SARS-CoV-1 modulates host translation machinery / positive regulation of GTPase activity / mTORC1-mediated signalling / TOR signaling

Domain/homology:

ATP-dependent RNA helicase eIF4A, DEAD-box helicase domain / EIF3I / Eukaryotic translation initiation factor 3 subunit G / Eukaryotic translation initiation factor 3 subunit G, N-terminal / eIF3G, RNA recognition motif / Eukaryotic translation initiation factor 3 subunit G / Eukaryotic translation initiation factor 3 subunit I / Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit 7 (eIF-3) / Eukaryotic translation initiation factor 3 subunit H / eIF3h, C-terminal / C-terminal region of eIF3h / Eukaryotic translation initiation factor 3 subunit B / eIF3B, RNA recognition motif / Eukaryotic translation initiation factor 3 subunit F / Translation initiation factor, beta propellor-like domain / Translation initiation factor 3 complex subunit L / RNA polymerase I-associated factor PAF67 / Eukaryotic translation initiation factor 3 subunit M / eIF3 subunit M, C-terminal helix domain / : / Eukaryotic translation initiation factor eIF2A / eIF3 subunit 6 N terminal domain / eIF3 subunit M, C-terminal helix / Eukaryotic translation initiation factor 3 subunit E, C-terminal / EIF3CL-like, C-terminal domain / Eukaryotic translation initiation factor 3 subunit E / Eukaryotic translation initiation factor 3 subunit E, N-terminal / eIF3 subunit 6 N terminal domain / Eukaryotic translation initiation factor 3 subunit K / Translation initiation factor 3, subunit 12, N-terminal, eukaryotic / : / eIF3a, PCI domain, TPR-like region / Eukaryotic translation initiation factor 3 subunit A / Eukaryotic translation initiation factor 3 subunit C, N-terminal domain / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit 8 N-terminus / Translation initiation factor IF2/IF5 domain / Translation initiation factor IF2/IF5, N-terminal / Translation initiation factor IF2/IF5, zinc-binding / Translation initiation factor IF2/IF5 / Domain found in IF2B/IF5 / domain present in translation initiation factor eIF2B and eIF5 / eIF4-gamma/eIF5/eIF2-epsilon / Domain at the C-termini of GCD6, eIF-2B epsilon, eIF-4 gamma and eIF-5 / W2 domain / W2 domain profile. / Translation initiation factor 1A (eIF-1A), conserved site / Eukaryotic initiation factor 1A signature. / Eukaryotic translation initiation factor 3 subunit M eIF3m/COP9 signalosome complex subunit 7 COPS7 / eukaryotic translation initiation factor 1A / Translation initiation factor 1A (eIF-1A) / Initiation factor eIF-4 gamma, MA3 / MA3 domain / MI domain profile. / Domain in DAP-5, eIF4G, MA-3 and other proteins. / MIF4G domain / Initiation factor eIF2 gamma, C-terminal / Initiation factor eIF2 gamma, domain 2 / Initiation factor eIF2 gamma, GTP-binding domain / Initiation factor eIF2 gamma, C terminal / Translation initiation factor 2, alpha subunit / Translation initiation factor 2, alpha subunit, middle domain superfamily / Translation initiation factor 2, alpha subunit, C-terminal / IF2a, S1-like domain / : / Eukaryotic translation initiation factor 2 alpha subunit / Middle domain of eukaryotic initiation factor 4G (eIF4G) / MIF4G-like, type 3 / RNA-binding domain, S1, IF1 type / Translation initiation factor 1A / IF-1 / S1 domain IF1 type profile. / DEAD-box subfamily ATP-dependent helicases signature. / ATP-dependent RNA helicase DEAD-box, conserved site / RNA helicase, DEAD-box type, Q motif / CSN8/PSMD8/EIF3K / CSN8/PSMD8/EIF3K family / DEAD-box RNA helicase Q motif profile. / Rpn11/EIF3F, C-terminal / Maintenance of mitochondrial structure and function / : / motif in proteasome subunits, Int-6, Nip-1 and TRIP-15 / PCI domain / Proteasome component (PCI) domain / PCI domain profile. / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / 40S ribosomal protein SA / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / Ubiquitin-like protein FUBI / JAB1/Mov34/MPN/PAD-1 ubiquitin protease / S1 domain profile. / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / : / Ribosomal protein S12e signature.

Component:

Eukaryotic translation initiation factor 3 subunit F / Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit H / Eukaryotic translation initiation factor 3 subunit G / Eukaryotic translation initiation factor 2 subunit 1 / Small ribosomal subunit protein eS17 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS5 / Eukaryotic translation initiation factor 2 subunit 2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein eS12 / Small ribosomal subunit protein eS19 / Eukaryotic translation initiation factor 2 subunit 3 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS10 / Eukaryotic translation initiation factor 1A, X-chromosomal / Eukaryotic translation initiation factor 5 / Eukaryotic translation initiation factor 3 subunit B / Eukaryotic translation initiation factor 3 subunit E / Eukaryotic initiation factor 4A-I / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein eS7 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein eS4, X isoform / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein eS24 / Small ribosomal subunit protein eS25 / Small ribosomal subunit protein eS26 / Small ribosomal subunit protein eS28 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein eS32 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein eS21 / Small ribosomal subunit protein RACK1 / Eukaryotic translation initiation factor 4 gamma 1 / Eukaryotic translation initiation factor 3 subunit I / Eukaryotic translation initiation factor 3 subunit A / Eukaryotic translation initiation factor 3 subunit M / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit K / Eukaryotic translation initiation factor 3 subunit L

• Biological species: Homo sapiens (human)
• Method: single particle reconstruction / cryo EM / Resolution: 3.5 Å
• Authors: Brito Querido J / Sokabe M / Diaz-Lopez I / Gordiyenko Y / Fraser CS / Ramakrishnan V

Funding support

United Kingdom, United States, 3 items

Organization	Grant number	Country
UK Research and Innovation (UKRI)	MC_U105184332	United Kingdom
Wellcome Trust	WT096570	United Kingdom
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	GM092927	United States

• Citation: Journal: Nat Struct Mol Biol / Year: 2024; Title: The structure of a human translation initiation complex reveals two independent roles for the helicase eIF4A.; Authors: Jailson Brito Querido / Masaaki Sokabe / Irene Díaz-López / Yuliya Gordiyenko / Christopher S Fraser / V Ramakrishnan / ; Abstract: Eukaryotic translation initiation involves recruitment of the 43S pre-initiation complex to the 5' end of mRNA by the cap-binding complex eIF4F, forming the 48S translation initiation complex (48S), which then scans along the mRNA until the start codon is recognized. We have previously shown that eIF4F binds near the mRNA exit channel of the 43S, leaving open the question of how mRNA secondary structure is removed as it enters the mRNA channel on the other side of the 40S subunit. Here we report the structure of a human 48S that shows that, in addition to the eIF4A that is part of eIF4F, there is a second eIF4A helicase bound at the mRNA entry site, which could unwind RNA secondary structures as they enter the 48S. The structure also reveals conserved interactions between eIF4F and the 43S, probaby explaining how eIF4F can promote mRNA recruitment in all eukaryotes.

History

Deposition	May 6, 2023	-
Header (metadata) release	Feb 7, 2024	-
Map release	Feb 7, 2024	-
Update	Apr 24, 2024	-
Current status	Apr 24, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_17297.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Human 48S complex
• Voxel size: X=Y=Z: 1.06 Å

Density

Contour Level	By AUTHOR: 0.006
Minimum - Maximum	-0.023388347 - 0.0645033
Average (Standard dev.)	0.0003936971 (±0.0022750173)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 400 400 400 Spacing 400 400 400
Cell	A=B=C: 423.99997 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_17297_msk_1.map

Additional map: Body1 - 3D multi-body refinement of 48S

• File: emd_17297_additional_1.map
• Annotation: Body1 - 3D multi-body refinement of 48S

Additional map: eIF3 after focus 4F

• File: emd_17297_additional_10.map
• Annotation: eIF3_after_focus_4F

Additional map: PIC after focus EntrySite

• File: emd_17297_additional_11.map
• Annotation: PIC_after_focus_EntrySite

Additional map: Body2 - 3D multi-body refinement of 48S

• File: emd_17297_additional_2.map
• Annotation: Body2 - 3D multi-body refinement of 48S

Additional map: Body3 - 3D multi-body refinement of 48S

• File: emd_17297_additional_3.map
• Annotation: Body3 - 3D multi-body refinement of 48S

Additional map: PIC after focus EntrySite Resampled map

• File: emd_17297_additional_4.map
• Annotation: PIC_after_focus_EntrySite Resampled map

Additional map: eIF4F-eIF3 after focus EntrySite Resampled map

• File: emd_17297_additional_5.map
• Annotation: eIF4F-eIF3_after_focus_EntrySite Resampled map

Additional map: eIF3 after focus 4F Resampled map

• File: emd_17297_additional_6.map
• Annotation: eIF3_after_focus_4F Resampled map

Additional map: eIF4F after focus 4F Resampled map

• File: emd_17297_additional_7.map
• Annotation: eIF4F_after_focus_4F Resampled map

Additional map: eIF4F eIF3 after focus 4F

• File: emd_17297_additional_8.map
• Annotation: eIF4F_eIF3 after_focus_4F

Additional map: eIF4F after focus 4F

• File: emd_17297_additional_9.map
• Annotation: eIF4F_after_focus_4F

Half map: Half map1 48S

• File: emd_17297_half_map_1.map
• Annotation: Half map1 48S

Half map: Half map1 48S

• File: emd_17297_half_map_2.map
• Annotation: Half map1 48S

Sample components

Entire : Human 48S translation initiation complex

• Entire: Name: Human 48S translation initiation complex

Components

• Complex: Human 48S translation initiation complex
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit E
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit F
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit G
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit H
  • Protein or peptide: 60S ribosomal protein L41
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit A
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit K
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit M
  • Protein or peptide: Eukaryotic translation initiation factor 2 subunit 1
  • Protein or peptide: Eukaryotic translation initiation factor 2 subunit 3
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit I
  • Protein or peptide: Eukaryotic translation initiation factor 1A, X-chromosomal
  • Protein or peptide: Eukaryotic translation initiation factor 5
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit B
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit C
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit L
  • Protein or peptide: 40S ribosomal protein S7
  • Protein or peptide: 40S ribosomal protein S27
  • Protein or peptide: 40S ribosomal protein S21
  • Protein or peptide: 40S ribosomal protein S2
  • Protein or peptide: 40S ribosomal protein S3a
  • Protein or peptide: 40S ribosomal protein SA
  • Protein or peptide: 40S ribosomal protein S26
  • Protein or peptide: 40S ribosomal protein S6
  • Protein or peptide: 40S ribosomal protein S14
  • Protein or peptide: Eukaryotic translation initiation factor 2 subunit 2
  • Protein or peptide: 40S ribosomal protein S13
  • RNA: 18S rRNA
  • Protein or peptide: 40S ribosomal protein S11
  • Protein or peptide: 40S ribosomal protein S4, X isoform
  • Protein or peptide: 40S ribosomal protein S9
  • Protein or peptide: 40S ribosomal protein S23
  • Protein or peptide: 40S ribosomal protein S30
  • Protein or peptide: 40S ribosomal protein S15a
  • Protein or peptide: 40S ribosomal protein S8
  • Protein or peptide: 40S ribosomal protein S24
  • Protein or peptide: 40S ribosomal protein S5
  • Protein or peptide: 40S ribosomal protein S16
  • Protein or peptide: 40S ribosomal protein S3
  • Protein or peptide: 40S ribosomal protein S10
  • Protein or peptide: 40S ribosomal protein S15
  • Protein or peptide: Receptor of activated protein C kinase 1
  • Protein or peptide: 40S ribosomal protein S19
  • Protein or peptide: 40S ribosomal protein S25
  • Protein or peptide: Small ribosomal subunit protein uS13
  • Protein or peptide: 40S ribosomal protein S29
  • Protein or peptide: Ubiquitin-40S ribosomal protein S27a
  • Protein or peptide: 40S ribosomal protein S12
  • Protein or peptide: 40S ribosomal protein S28
  • Protein or peptide: 40S ribosomal protein S17
  • Protein or peptide: 40S ribosomal protein S20
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit D
  • RNA: tRNAiMet
  • RNA: mRNA
  • Protein or peptide: Eukaryotic initiation factor 4A-I
  • Protein or peptide: Eukaryotic translation initiation factor 4 gamma 1
• Ligand: ZINC ION
• Ligand: MAGNESIUM ION

Supramolecule #1: Human 48S translation initiation complex

• Supramolecule: Name: Human 48S translation initiation complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#56
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: Eukaryotic translation initiation factor 3 subunit E

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit E / type: protein_or_peptide / ID: 1 ; Details: Eukaryotic translation initiation factor 3 subunit E; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 52.281633 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MAEYDLTTRI AHFLDRHLVF PLLEFLSVKE IYNEKELLQG KLDLLSDTNM VDFAMDVYKN LYSDDIPHAL REKRTTVVAQ LKQLQAETE PIVKMFEDPE TTRQMQSTRD GRMLFDYLAD KHGFRQEYLD TLYRYAKFQY ECGNYSGAAE YLYFFRVLVP A TDRNALSS LWGKLASEIL MQNWDAAMED LTRLKETIDN NSVSSPLQSL QQRTWLIHWS LFVFFNHPKG RDNIIDLFLY QP QYLNAIQ TMCPHILRYL TTAVITNKDV RKRRQVLKDL VKVIQQESYT YKDPITEFVE CLYVNFDFDG AQKKLRECES VLV NDFFLV ACLEDFIENA RLFIFETFCR IHQCISINML ADKLNMTPEE AERWIVNLIR NARLDAKIDS KLGHVVMGNN AVSP YQQVI EKTKSLSFRS QMLAMNIEKK LNQNSRSEAP NWATQDSGFY

UniProtKB: Eukaryotic translation initiation factor 3 subunit E

Macromolecule #2: Eukaryotic translation initiation factor 3 subunit F

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit F / type: protein_or_peptide / ID: 2 ; Details: Eukaryotic translation initiation factor 3 subunit F; Number of copies: 1 / Enantiomer: LEVO / EC number: ubiquitinyl hydrolase 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 37.593645 KDa

Sequence

String:

MATPAVPVSA PPATPTPVPA AAPASVPAPT PAPAAAPVPA AAPASSSDPA AAAAATAAPG QTPASAQAPA QTPAPALPGP ALPGPFPGG RVVRLHPVIL ASIVDSYERR NEGAARVIGT LLGTVDKHSV EVTNCFSVPH NESEDEVAVD MEFAKNMYEL H KKVSPNEL ILGWYATGHD ITEHSVLIHE YYSREAPNPI HLTVDTSLQN GRMSIKAYVS TLMGVPGRTM GVMFTPLTVK YA YYDTERI GVDLIMKTCF SPNRVIGLSS DLQQVGGASA RIQDALSTVL QYAEDVLSGK VSADNTVGRF LMSLVNQVPK IVP DDFETM LNSNINDLLM VTYLANLTQS QIALNEKLVN L

UniProtKB: Eukaryotic translation initiation factor 3 subunit F

Macromolecule #3: Eukaryotic translation initiation factor 3 subunit G

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit G / type: protein_or_peptide / ID: 3 ; Details: Eukaryotic translation initiation factor 3 subunit G; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.662016 KDa

Sequence

String:

MPTGDFDSKP SWADQVEEEG EDDKCVTSEL LKGIPLATGD TSPEPELLPG APLPPPKEVI NGNIKTVTEY KIDEDGKKFK IVRTFRIET RKASKAVARR KNWKKFGNSE FDPPGPNVAT TTVSDDVSMT FITSKEDLNC QEEEDPMNKL KGQKIVSCRI C KGDHWTTR CPYKDTLGPM QKELAEQLGL STGEKEKLPG ELEPVQATQN KTGKYVPPSL RDGASRRGES MQPNRRADDN AT IRVTNLS EDTRETDLQE LFRPFGSISR IYLAKDKTTG QSKGFAFISF HRREDAARAI AGVSGFGYDH LILNVEWAKP STN

UniProtKB: Eukaryotic translation initiation factor 3 subunit G

Macromolecule #4: Eukaryotic translation initiation factor 3 subunit H

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit H / type: protein_or_peptide / ID: 4 ; Details: Eukaryotic translation initiation factor 3 subunit H; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 39.979277 KDa

Sequence

String:

MASRKEGTGS TATSSSSTAG AAGKGKGKGG SGDSAVKQVQ IDGLVVLKII KHYQEEGQGT EVVQGVLLGL VVEDRLEITN CFPFPQHTE DDADFDEVQY QMEMMRSLRH VNIDHLHVGW YQSTYYGSFV TRALLDSQFS YQHAIEESVV LIYDPIKTAQ G SLSLKAYR LTPKLMEVCK EKDFSPEALK KANITFEYMF EEVPIVIKNS HLINVLMWEL EKKSAVADKH ELLSLASSNH LG KNLQLLM DRVDEMSQDI VKYNTYMRNT SKQQQQKHQY QQRRQQENMQ RQSRGEPPLP EEDLSKLFKP PQPPARMDSL LIA GQINTY CQNIKEFTAQ NLGKLFMAQA LQEYNN

UniProtKB: Eukaryotic translation initiation factor 3 subunit H

Macromolecule #5: 60S ribosomal protein L41

• Macromolecule: Name: 60S ribosomal protein L41 / type: protein_or_peptide / ID: 5 / Details: 60S ribosomal protein L41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 3.473451 KDa

Sequence

String:

MRAKWRKKRM RRLKRKRRKM RQRSK

UniProtKB: Small ribosomal subunit protein eS32

Macromolecule #6: Eukaryotic translation initiation factor 3 subunit A

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit A / type: protein_or_peptide / ID: 6 ; Details: Eukaryotic translation initiation factor 3 subunit A; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 166.903781 KDa

Sequence

String:

MPAYFQRPEN ALKRANEFLE VGKKQPALDV LYDVMKSKKH RTWQKIHEPI MLKYLELCVD LRKSHLAKEG LYQYKNICQQ VNIKSLEDV VRAYLKMAEE KTEAAKEESQ QMVLDIEDLD NIQTPESVLL SAVSGEDTQD RTDRLLLTPW VKFLWESYRQ C LDLLRNNS RVERLYHDIA QQAFKFCLQY TRKAEFRKLC DNLRMHLSQI QRHHNQSTAI NLNNPESQSM HLETRLVQLD SA ISMELWQ EAFKAVEDIH GLFSLSKKPP KPQLMANYYN KVSTVFWKSG NALFHASTLH RLYHLSREMR KNLTQDEMQR MST RVLLAT LSIPITPERT DIARLLDMDG IIVEKQRRLA TLLGLQAPPT RIGLINDMVR FNVLQYVVPE VKDLYNWLEV EFNP LKLCE RVTKVLNWVR EQPEKEPELQ QYVPQLQNNT ILRLLQQVSQ IYQSIEFSRL TSLVPFVDAF QLERAIVDAA RHCDL QVRI DHTSRTLSFG SDLNYATRED APIGPHLQSM PSEQIRNQLT AMSSVLAKAL EVIKPAHILQ EKEEQHQLAV TAYLKN SRK EHQRILARRQ TIEERKERLE SLNIQREKEE LEQREAELQK VRKAEEERLR QEAKEREKER ILQEHEQIKK KTVRERL EQ IKKTELGAKA FKDIDIEDLE ELDPDFIMAK QVEQLEKEKK ELQERLKNQE KKIDYFERAK RLEEIPLIKS AYEEQRIK D MDLWEQQEEE RITTMQLERE KALEHKNRMS RMLEDRDLFV MRLKAARQSV YEEKLKQFEE RLAEERHNRL EERKRQRKE ERRITYYREK EEEEQRRAEE QMLKEREERE RAERAKREEE LREYQERVKK LEEVERKKRQ RELEIEERER RREEERRLGD SSLSRKDSR WGDRDSEGTW RKGPEADSEW RRGPPEKEWR RGEGRDEDRS HRRDEERPRR LGDDEDREPS LRPDDDRVPR R GMDDDRGP RRGPEEDRFS RRGADDDRPS WRNTDDDRPP RRIADEDRGN WRHADDDRPP RRGLDEDRGS WRTADEDRGP RR GMDDDRG PRRGGADDER SSWRNADDDR GPRRGLDDDR GPRRGMDDDR GPRRGMDDDR GPRRGMDDDR GPRRGLDDDR GPW RNADDD RIPRRGAEDD RGPWRNMDDD RLSRRADDDR FPRRGDDSRP GPWRPLVKPG GWREKEKARE ESWGPPRESR PSEE REWDR EKERDRDNQD REENDKDPER ERDRERDVDR EDRFRRPRDE GGWRRGPAEE SSSWRDSSRR DDRDRDDRRR ERDDR RDLR ERRDLRDDRD RRGPPLRSER EEVSSWRRAD DRKDDRVEER DPPRRVPPPA LSRDRERDRD REREGEKEKA SWRAEK DRE SLRRTKNETD EDGWTTVRR

UniProtKB: Eukaryotic translation initiation factor 3 subunit A

Macromolecule #7: Eukaryotic translation initiation factor 3 subunit K

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit K / type: protein_or_peptide / ID: 7 ; Details: Eukaryotic translation initiation factor 3 subunit K; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 25.083619 KDa

Sequence

String:

MAMFEQMRAN VGKLLKGIDR YNPENLATLE RYVETQAKEN AYDLEANLAV LKLYQFNPAF FQTTVTAQIL LKALTNLPHT DFTLCKCMI DQAHQEERPI RQILYLGDLL ETCHFQAFWQ ALDENMDLLE GITGFEDSVR KFICHVVGIT YQHIDRWLLA E MLGDLSDS QLKVWMSKYG WSADESGQIF ICSQEESIKP KNIVEKIDFD SVSSIMASSQ

UniProtKB: Eukaryotic translation initiation factor 3 subunit K

Macromolecule #8: Eukaryotic translation initiation factor 3 subunit M

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit M / type: protein_or_peptide / ID: 8 ; Details: Eukaryotic translation initiation factor 3 subunit M; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 42.555832 KDa

Sequence

String:

MSVPAFIDIS EEDQAAELRA YLKSKGAEIS EENSEGGLHV DLAQIIEACD VCLKEDDKDV ESVMNSVVSL LLILEPDKQE ALIESLCEK LVKFREGERP SLRLQLLSNL FHGMDKNTPV RYTVYCSLIK VAASCGAIQY IPTELDQVRK WISDWNLTTE K KHTLLRLL YEALVDCKKS DAASKVMVEL LGSYTEDNAS QARVDAHRCI VRALKDPNAF LFDHLLTLKP VKFLEGELIH DL LTIFVSA KLASYVKFYQ NNKDFIDSLG LLHEQNMAKM RLLTFMGMAV ENKEISFDTM QQELQIGADD VEAFVIDAVR TKM VYCKID QTQRKVVVSH STHRTFGKQQ WQQLYDTLNA WKQNLNKVKN SLLSLSDT

UniProtKB: Eukaryotic translation initiation factor 3 subunit M

Macromolecule #9: Eukaryotic translation initiation factor 2 subunit 1

• Macromolecule: Name: Eukaryotic translation initiation factor 2 subunit 1 / type: protein_or_peptide / ID: 9 ; Details: Eukaryotic translation initiation factor 2 subunit 1; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 36.16118 KDa

Sequence

String:

MPGLSCRFYQ HKFPEVEDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE LSRRRIRSIN KLIRIGRNEC VVVIRVDKEK GYIDLSKRR VSPEEAIKCE DKFTKSKTVY SILRHVAEVL EYTKDEQLES LFQRTAWVFD DKYKRPGYGA YDAFKHAVSD P SILDSLDL NEDEREVLIN NINRRLTPQA VKIRADIEVA CYGYEGIDAV KEALRAGLNC STENMPIKIN LIAPPRYVMT TT TLERTEG LSVLSQAMAV IKEKIEEKRG VFNVQMEPKV VTDTDETELA RQMERLEREN AEVDGDDDAE EMEAKAED

UniProtKB: Eukaryotic translation initiation factor 2 subunit 1

Macromolecule #10: Eukaryotic translation initiation factor 2 subunit 3

• Macromolecule: Name: Eukaryotic translation initiation factor 2 subunit 3 / type: protein_or_peptide / ID: 10 ; Details: Eukaryotic translation initiation factor 2 subunit 3; Number of copies: 1 / Enantiomer: LEVO / EC number: protein-synthesizing GTPase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 51.178406 KDa

Sequence

String:

MAGGEAGVTL GQPHLSRQDL TTLDVTKLTP LSHEVISRQA TINIGTIGHV AHGKSTVVKA ISGVHTVRFK NELERNITIK LGYANAKIY KLDDPSCPRP ECYRSCGSST PDEFPTDIPG TKGNFKLVRH VSFVDCPGHD ILMATMLNGA AVMDAALLLI A GNESCPQP QTSEHLAAIE IMKLKHILIL QNKIDLVKES QAKEQYEQIL AFVQGTVAEG APIIPISAQL KYNIEVVCEY IV KKIPVPP RDFTSEPRLI VIRSFDVNKP GCEVDDLKGG VAGGSILKGV LKVGQEIEVR PGIVSKDSEG KLMCKPIFSK IVS LFAEHN DLQYAAPGGL IGVGTKIDPT LCRADRMVGQ VLGAVGALPE IFTELEISYF LLRRLLGVRT EGDKKAAKVQ KLSK NEVLM VNIGSLSTGG RVSAVKADLG KIVLTNPVCT EVGEKIALSR RVEKHWRLIG WGQIRRGVTI KPTVDDD

UniProtKB: Eukaryotic translation initiation factor 2 subunit 3

Macromolecule #11: Eukaryotic translation initiation factor 3 subunit I

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit I / type: protein_or_peptide / ID: 11 ; Details: Eukaryotic translation initiation factor 3 subunit I; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 36.543773 KDa

Sequence

String:

MKPILLQGHE RSITQIKYNR EGDLLFTVAK DPIVNVWYSV NGERLGTYMG HTGAVWCVDA DWDTKHVLTG SADNSCRLWD CETGKQLAL LKTNSAVRTC GFDFGGNIIM FSTDKQMGYQ CFVSFFDLRD PSQIDNNEPY MKIPCNDSKI TSAVWGPLGE C IIAGHESG ELNQYSAKSG EVLVNVKEHS RQINDIQLSR DMTMFVTASK DNTAKLFDST TLEHQKTFRT ERPVNSAALS PN YDHVVLG GGQEAMDVTT TSTRIGKFEA RFFHLAFEEE FGRVKGHFGP INSVAFHPDG KSYSSGGEDG YVRIHYFDPQ YFE FEFEA

UniProtKB: Eukaryotic translation initiation factor 3 subunit I

Macromolecule #12: Eukaryotic translation initiation factor 1A, X-chromosomal

• Macromolecule: Name: Eukaryotic translation initiation factor 1A, X-chromosomal; type: protein_or_peptide / ID: 12 / Details: Eukaryotic translation initiation factor 1A / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.488449 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MPKNKGKGGK NRRRGKNENE SEKRELVFKE DGQEYAQVIK MLGNGRLEAM CFDGVKRLCH IRGKLRKKVW INTSDIILVG LRDYQDNKA DVILKYNADE ARSLKAYGEL PEHAKINETD TFGPGDDDEI QFDDIGDDDE DIDDI

UniProtKB: Eukaryotic translation initiation factor 1A, X-chromosomal

Macromolecule #13: Eukaryotic translation initiation factor 5

• Macromolecule: Name: Eukaryotic translation initiation factor 5 / type: protein_or_peptide / ID: 13 / Details: Eukaryotic translation initiation factor 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 49.30175 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MSVNVNRSVS DQFYRYKMPR LIAKVEGKGN GIKTVIVNMV DVAKALNRPP TYPTKYFGCE LGAQTQFDVK NDRYIVNGSH EANKLQDML DGFIKKFVLC PECENPETDL HVNPKKQTIG NSCKACGYRG MLDTHHKLCT FILKNPPENS DSGTGKKEKE K KNRKGKDK ENGSVSSSET PPPPPPPNEI NPPPHTMEEE EDDDWGEDTT EEAQRRRMDE ISDHAKVLTL SDDLERTIEE RV NILFDFV KKKKEEGVID SSDKEIVAEA ERLDVKAMGP LVLTEVLFNE KIREQIKKYR RHFLRFCHNN KKAQRYLLHG LEC VVAMHQ AQLISKIPHI LKEMYDADLL EEEVIISWSE KASKKYVSKE LAKEIRVKAE PFIKWLKEAE EESSGGEEED EDEN IEVVY SKAASVPKVE TVKSDNKDDD IDIDAI

UniProtKB: Eukaryotic translation initiation factor 5

Macromolecule #14: Eukaryotic translation initiation factor 3 subunit B

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit B / type: protein_or_peptide / ID: 14 ; Details: Eukaryotic translation initiation factor 3 subunit B; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 92.593414 KDa

Sequence

String:

MQDAENVAVP EAAEERAEPG QQQPAAEPPP AEGLLRPAGP GAPEAAGTEA SSEEVGIAEA GPESEVRTEP AAEAEAASGP SESPSPPAA EELPGSHAEP PVPAQGEAPG EQARDERSDS RAQAVSEDAG GNEGRAAEAE PRALENGDAD EPSFSDPEDF V DDVSEEEL LGDVLKDRPQ EADGIDSVIV VDNVPQVGPD RLEKLKNVIH KIFSKFGKIT NDFYPEEDGK TKGYIFLEYA SP AHAVDAV KNADGYKLDK QHTFRVNLFT DFDKYMTISD EWDIPEKQPF KDLGNLRYWL EEAECRDQYS VIFESGDRTS IFW NDVKDP VSIEERARWT ETYVRWSPKG TYLATFHQRG IALWGGEKFK QIQRFSHQGV QLIDFSPCER YLVTFSPLMD TQDD PQAII IWDILTGHKK RGFHCESSAH WPIFKWSHDG KFFARMTLDT LSIYETPSMG LLDKKSLKIS GIKDFSWSPG GNIIA FWVP EDKDIPARVT LMQLPTRQEI RVRNLFNVVD CKLHWQKNGD YLCVKVDRTP KGTQGVVTNF EIFRMREKQV PVDVVE MKE TIIAFAWEPN GSKFAVLHGE APRISVSFYH VKNNGKIELI KMFDKQQANT IFWSPQGQFV VLAGLRSMNG ALAFVDT SD CTVMNIAEHY MASDVEWDPT GRYVVTSVSW WSHKVDNAYW LWTFQGRLLQ KNNKDRFCQL LWRPRPPTLL SQEQIKQI K KDLKKYSKIF EQKDRLSQSK ASKELVERRR TMMEDFRKYR KMAQELYMEQ KNERLELRGG VDTDELDSNV DDWEEETIE FFVTEEIIPL GNQE

UniProtKB: Eukaryotic translation initiation factor 3 subunit B

Macromolecule #15: Eukaryotic translation initiation factor 3 subunit C

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit C / type: protein_or_peptide / ID: 15 ; Details: Eukaryotic translation initiation factor 3 subunit C; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 105.503945 KDa

Sequence

String:

MSRFFTTGSD SESESSLSGE ELVTKPVGGN YGKQPLLLSE DEEDTKRVVR SAKDKRFEEL TNLIRTIRNA MKIRDVTKCL EEFELLGKA YGKAKSIVDK EGVPRFYIRI LADLEDYLNE LWEDKEGKKK MNKNNAKALS TLRQKIRKYN RDFESHITSY K QNPEQSAD EDAEKNEEDS EGSSDEDEDE DGVSAATFLK KKSEAPSGES RKFLKKMDDE DEDSEDSEDD EDWDTGSTSS DS DSEEEEG KQTALASRFL KKAPTTDEDK KAAEKKREDK AKKKHDRKSK RLDEEEEDNE GGEWERVRGG VPLVKEKPKM FAK GTEITH AVVIKKLNEI LQARGKKGTD RAAQIELLQL LVQIAAENNL GEGVIVKIKF NIIASLYDYN PNLATYMKPE MWGK CLDCI NELMDILFAN PNIFVGENIL EESENLHNAD QPLRVRGCIL TLVERMDEEF TKIMQNTDPH SQEYVEHLKD EAQVC AIIE RVQRYLEEKG TTEEVCRIYL LRILHTYYKF DYKAHQRQLT PPEGSSKSEQ DQAENEGEDS AVLMERLCKY IYAKDR TDR IRTCAILCHI YHHALHSRWY QARDLMLMSH LQDNIQHADP PVQILYNRTM VQLGICAFRQ GLTKDAHNAL LDIQSSG RA KELLGQGLLL RSLQERNQEQ EKVERRRQVP FHLHINLELL ECVYLVSAML LEIPYMAAHE SDARRRMISK QFHHQLRV G ERQPLLGPPE SMREHVVAAS KAMKMGDWKT CHSFIINEKM NGKVWDLFPE ADKVRTMLVR KIQEESLRTY LFTYSSVYD SISMETLSDM FELDLPTVHS IISKMIINEE LMASLDQPTQ TVVMHRTEPT AQQNLALQLA EKLGSLVENN ERVFDHKQGT YGGYFRDQK DGYRKNEGYM RRGGYRQQQS QTAY

UniProtKB: Eukaryotic translation initiation factor 3 subunit C

Macromolecule #16: Eukaryotic translation initiation factor 3 subunit L

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit L / type: protein_or_peptide / ID: 16 ; Details: Eukaryotic translation initiation factor 3 subunit L; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 66.803734 KDa

Sequence

String:

MSYPADDYES EAAYDPYAYP SDYDMHTGDP KQDLAYERQY EQQTYQVIPE VIKNFIQYFH KTVSDLIDQK VYELQASRVS SDVIDQKVY EIQDIYENSW TKLTERFFKN TPWPEAEAIA PQVGNDAVFL ILYKELYYRH IYAKVSGGPS LEQRFESYYN Y CNLFNYIL NADGPAPLEL PNQWLWDIID EFIYQFQSFS QYRCKTAKKS EEEIDFLRSN PKIWNVHSVL NVLHSLVDKS NI NRQLEVY TSGGDPESVA GEYGRHSLYK MLGYFSLVGL LRLHSLLGDY YQAIKVLENI ELNKKSMYSR VPECQVTTYY YVG FAYLMM RRYQDAIRVF ANILLYIQRT KSMFQRTTYK YEMINKQNEQ MHALLAIALT MYPMRIDESI HLQLREKYGD KMLR MQKGD PQVYEELFSY SCPKFLSPVV PNYDNVHPNY HKEPFLQQLK VFSDEVQQQA QLSTIRSFLK LYTTMPVAKL AGFLD LTEQ EFRIQLLVFK HKMKNLVWTS GISALDGEFQ SASEVDFYID KDMIHIADTK VARRYGDFFI RQIHKFEELN RTLKKM GQR P

UniProtKB: Eukaryotic translation initiation factor 3 subunit L

Macromolecule #17: 40S ribosomal protein S7

• Macromolecule: Name: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 17 / Details: Small ribosomal subunit protein eS7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.168914 KDa

Sequence

String:

MFSSSAKIVK PNGEKPDEFE SGISQALLEL EMNSDLKAQL RELNITAAKE IEVGGGRKAI IIFVPVPQLK SFQKIQVRLV RELEKKFSG KHVVFIAQRR ILPKPTRKSR TKNKQKRPRS RTLTAVHDAI LEDLVFPSEI VGKRIRVKLD GSRLIKVHLD K AQQNNVEH KVETFSGVYK KLTGKDVNFE FPEFQL

UniProtKB: Small ribosomal subunit protein eS7

Macromolecule #18: 40S ribosomal protein S27

• Macromolecule: Name: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 18 / Details: Small ribosomal subunit protein eS27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.480186 KDa

Sequence

String:

MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH

UniProtKB: Small ribosomal subunit protein eS27

Macromolecule #19: 40S ribosomal protein S21

• Macromolecule: Name: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 19 / Details: Small ribosomal subunit protein eS21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.124389 KDa

Sequence

String:

MQNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKADGIVS KNF

UniProtKB: Small ribosomal subunit protein eS21

Macromolecule #20: 40S ribosomal protein S2

• Macromolecule: Name: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 20 / Details: Small ribosomal subunit protein uS5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 31.376516 KDa

Sequence

String:

MADDAGAAGG PGGPGGPGMG NRGGFRGGFG SGIRGRGRGR GRGRGRGRGA RGGKAEDKEW MPVTKLGRLV KDMKIKSLEE IYLFSLPIK ESEIIDFFLG ASLKDEVLKI MPVQKQTRAG QRTRFKAFVA IGDYNGHVGL GVKCSKEVAT AIRGAIILAK L SIVPVRRG YWGNKIGKPH TVPCKVTGRC GSVLVRLIPA PRGTGIVSAP VPKKLLMMAG IDDCYTSARG CTATLGNFAK AT FDAISKT YSYLTPDLWK ETVFTKSPYQ EFTDHLVKTH TRVSVQRTQA PAVATT

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #21: 40S ribosomal protein S3a

• Macromolecule: Name: 40S ribosomal protein S3a / type: protein_or_peptide / ID: 21 / Details: Small ribosomal subunit protein eS1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 30.002061 KDa

Sequence

String:

MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM MEIMTREVQT NDLKEVVNKL IPDSIGKDIE KACQSIYPLH DVFVRKVKML KKPKFELGKL MELHGEGSSS GK ATGDETG AKVERADGYE PPVQESV

UniProtKB: Small ribosomal subunit protein eS1

Macromolecule #22: 40S ribosomal protein SA

• Macromolecule: Name: 40S ribosomal protein SA / type: protein_or_peptide / ID: 22 / Details: Small ribosomal subunit protein uS2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 32.883938 KDa

Sequence

String:

MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQAAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA HSVGLMWWML AREVLRMRGT ISREHPWEVM PDLYFYRDPE EIEKEEQAAA EKAVTKEEFQ GEWTAPAPEF TA TQPEVAD WSEGVQVPSV PIQQFPTEDW SAQPATEDWS AAPTAQATEW VGATTDWS

UniProtKB: Small ribosomal subunit protein uS2

Macromolecule #23: 40S ribosomal protein S26

• Macromolecule: Name: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 23 / Details: Small ribosomal subunit protein eS26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.047532 KDa

Sequence

String:

MTKKRRNNGR AKKGRGHVQP IRCTNCARCV PKDKAIKKFV IRNIVEAAAV RDISEASVFD AYVLPKLYVK LHYCVSCAIH SKVVRNRSR EARKDRTPPP RFRPAGAAPR PPPKPM

UniProtKB: Small ribosomal subunit protein eS26

Macromolecule #24: 40S ribosomal protein S6

• Macromolecule: Name: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 24 / Details: Small ribosomal subunit protein eS6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.751906 KDa

Sequence

String:

MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRRRLSSLR AS TSKSESS QK

UniProtKB: Small ribosomal subunit protein eS6

Macromolecule #25: 40S ribosomal protein S14

• Macromolecule: Name: 40S ribosomal protein S14 / type: protein_or_peptide / ID: 25 / Details: Small ribosomal subunit protein uS11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.302772 KDa

Sequence

String:

MAPRKGKEKK EEQVISLGPQ VAEGENVFGV CHIFASFNDT FVHVTDLSGK ETICRVTGGM KVKADRDESS PYAAMLAAQD VAQRCKELG ITALHIKLRA TGGNRTKTPG PGAQSALRAL ARSGMKIGRI EDVTPIPSDS TRRKGGRRGR RL

UniProtKB: Small ribosomal subunit protein uS11

Macromolecule #26: Eukaryotic translation initiation factor 2 subunit 2

• Macromolecule: Name: Eukaryotic translation initiation factor 2 subunit 2 / type: protein_or_peptide / ID: 26 ; Details: Eukaryotic translation initiation factor 2 subunit 2; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 38.454484 KDa

Sequence

String:

MSGDEMIFDP TMSKKKKKKK KPFMLDEEGD TQTEETQPSE TKEVEPEPTE DKDLEADEED TRKKDASDDL DDLNFFNQKK KKKKTKKIF DIDEAEEGVK DLKIESDVQE PTEPEDDLDI MLGNKKKKKK NVKFPDEDEI LEKDEALEDE DNKKDDGISF S NQTGPAWA GSERDYTYEE LLNRVFNIMR EKNPDMVAGE KRKFVMKPPQ VVRVGTKKTS FVNFTDICKL LHRQPKHLLA FL LAELGTS GSIDGNNQLV IKGRFQQKQI ENVLRRYIKE YVTCHTCRSP DTILQKDTRL YFLQCETCHS RCSVASIKTG FQA VTGKRA QLRAKAN

UniProtKB: Eukaryotic translation initiation factor 2 subunit 2

Macromolecule #27: 40S ribosomal protein S13

• Macromolecule: Name: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 27 / Details: Small ribosomal subunit protein uS15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.259389 KDa

Sequence

String:

MGRMHAPGKG LSQSALPYRR SVPTWLKLTS DDVKEQIYKL AKKGLTPSQI GVILRDSHGV AQVRFVTGNK ILRILKSKGL APDLPEDLY HLIKKAVAVR KHLERNRKDK DAKFRLILIE SRIHRLARYY KTKRVLPPNW KYESSTASAL VA

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #29: 40S ribosomal protein S11

• Macromolecule: Name: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 29 / Details: Small ribosomal subunit protein uS17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.468826 KDa

Sequence

String:

MADIQTERAY QKQPTIFQNK KRVLLGETGK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #30: 40S ribosomal protein S4, X isoform

• Macromolecule: Name: 40S ribosomal protein S4, X isoform / type: protein_or_peptide / ID: 30 / Details: Small ribosomal subunit protein eS4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.654869 KDa

Sequence

String:

MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE VKKICMQRFI KIDGKVRTDI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT I QIDLETGK ITDFIKFDTG NLCMVTGGAN LGRIGVITNR ERHPGSFDVV HVKDANGNSF ATRLSNIFVI GKGNKPWISL PR GKGIRLT IAEERDKRLA AKQSSG

UniProtKB: Small ribosomal subunit protein eS4, X isoform

Macromolecule #31: 40S ribosomal protein S9

• Macromolecule: Name: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 31 / Details: Small ribosomal subunit protein uS4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.641564 KDa

Sequence

String:

MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG RPGRVKRKNA KKGQGGAGAG DDEEED

UniProtKB: Small ribosomal subunit protein uS4

Macromolecule #32: 40S ribosomal protein S23

• Macromolecule: Name: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 32 / Details: Small ribosomal subunit protein uS12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.844666 KDa

Sequence

String:

MGKCRGLRTA RKLRSHRRDQ KWHDKQYKKA HLGTALKANP FGGASHAKGI VLEKVGVEAK QPNSAIRKCV RVQLIKNGKK ITAFVPNDG CLNFIEENDE VLVAGFGRKG HAVGDIPGVR FKVVKVANVS LLALYKGKKE RPRS

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #33: 40S ribosomal protein S30

• Macromolecule: Name: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 33 / Details: Small ribosomal subunit protein eS30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 6.668938 KDa

Sequence

String:

KVHGSLARAG KVRGQTPKVA KQEKKKKKTG RAKRRMQYNR RFVNVVPTFG KKKGPNANS

UniProtKB: Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein

Macromolecule #34: 40S ribosomal protein S15a

• Macromolecule: Name: 40S ribosomal protein S15a / type: protein_or_peptide / ID: 34 / Details: Small ribosomal subunit protein uS8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.865555 KDa

Sequence

String:

MVRMNVLADA LKSINNAEKR GKRQVLIRPC SKVIVRFLTV MMKHGYIGEF EIIDDHRAGK IVVNLTGRLN KCGVISPRFD VQLKDLEKW QNNLLPSRQF GFIVLTTSAG IMDHEEARRK HTGGKILGFF F

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #35: 40S ribosomal protein S8

• Macromolecule: Name: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 35 / Details: Small ribosomal subunit protein eS8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.263387 KDa

Sequence

String:

MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ GKLLACIASR PGQCGRADGY VLEGKELEFY LRKIKARKGK

UniProtKB: Small ribosomal subunit protein eS8

Macromolecule #36: 40S ribosomal protein S24

• Macromolecule: Name: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 36 / Details: Small ribosomal subunit protein eS24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.463333 KDa

Sequence

String:

MNDTVTIRTR KFMTNRLLQR KQMVIDVLHP GKATVPKTEI REKLAKMYKT TPDVIFVFGF RTHFGGGKTT GFGMIYDSLD YAKKNEPKH RLARHGLYEK KKTSRKQRKE RKNRMKKVRG TAKANVGAGK KPKE

UniProtKB: Small ribosomal subunit protein eS24

Macromolecule #37: 40S ribosomal protein S5

• Macromolecule: Name: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 37 / Details: Small ribosomal subunit protein uS7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.913453 KDa

Sequence

String:

MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT IAECLADELI NAAKGSSNSY AIKKKDELER VAKSNR

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #38: 40S ribosomal protein S16

• Macromolecule: Name: 40S ribosomal protein S16 / type: protein_or_peptide / ID: 38 / Details: Small ribosomal subunit protein uS9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.477377 KDa

Sequence

String:

MPSKGPLQSV QVFGRKKTAT AVAHCKRGNG LIKVNGRPLE MIEPRTLQYK LLEPVLLLGK ERFAGVDIRV RVKGGGHVAQ IYAIRQSIS KALVAYYQKY VDEASKKEIK DILIQYDRTL LVADPRRCES KKFGGPGARA RYQKSYR

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #39: 40S ribosomal protein S3

• Macromolecule: Name: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 39 / Details: Small ribosomal subunit protein uS3 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-(apurinic or apyrimidinic site) lyase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.729369 KDa

Sequence

String:

MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAE KVATRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFIMES GAKGCEVVVS GKLRGQRAKS MKFVDGLMIH S GDPVNYYV DTAVRHVLLR QGVLGIKVKI MLPWDPTGKI GPKKPLPDHV SIVEPKDEIL PTTPISEQKG GKPEPPAMPQ PV PTA

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #40: 40S ribosomal protein S10

• Macromolecule: Name: 40S ribosomal protein S10 / type: protein_or_peptide / ID: 40 / Details: Small ribosomal subunit protein eS10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.933846 KDa

Sequence

String:

MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSRP ETGRPRPKGL EGERPARLTR GEADRDTYRR SAVPPGADKK AEAGAGSATE FQFRGGFGRG R GQPPQ

UniProtKB: Small ribosomal subunit protein eS10

Macromolecule #41: 40S ribosomal protein S15

• Macromolecule: Name: 40S ribosomal protein S15 / type: protein_or_peptide / ID: 41 / Details: Small ribosomal subunit protein uS19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.076207 KDa

Sequence

String:

MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLNRGLR RKQHSLLKRL RKAKKEAPPM EKPEVVKTHL RDMIILPEM VGSMVGVYNG KTFNQVEIKP EMIGHYLGEF SITYKPVKHG RPGIGATHSS RFIPLK

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #42: Receptor of activated protein C kinase 1

• Macromolecule: Name: Receptor of activated protein C kinase 1 / type: protein_or_peptide / ID: 42 / Details: Receptor of activated protein C kinase 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.115652 KDa

Sequence

String:

MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC GWDKLVKVWN LANCKLKTNH IGHTGYLNTV TVSPDGSLCA SGGKDGQAML WDLNEGKHLY TLDGGDIINA LC FSPNRYW LCAATGPSIK IWDLEGKIIV DELKQEVIST SSKAEPPQCT SLAWSADGQT LFAGYTDNLV RVWQVTIGTR

UniProtKB: Small ribosomal subunit protein RACK1

Macromolecule #43: 40S ribosomal protein S19

• Macromolecule: Name: 40S ribosomal protein S19 / type: protein_or_peptide / ID: 43 / Details: Small ribosomal subunit protein eS19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.091562 KDa

Sequence

String:

MPGVTVKDVN QQEFVRALAA FLKKSGKLKV PEWVDTVKLA KHKELAPYDE NWFYTRAAST ARHLYLRGGA GVGSMTKIYG GRQRNGVMP SHFSRGSKSV ARRVLQALEG LKMVEKDQDG GRKLTPQGQR DLDRIAGQVA AANKKH

UniProtKB: Small ribosomal subunit protein eS19

Macromolecule #44: 40S ribosomal protein S25

• Macromolecule: Name: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 44 / Details: Small ribosomal subunit protein eS25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.776224 KDa

Sequence

String:

MPPKDDKKKK DAGKSAKKDK DPVNKSGGKA KKKKWSKGKV RDKLNNLVLF DKATYDKLCK EVPNYKLITP AVVSERLKIR GSLARAALQ ELLSKGLIKL VSKHRAQVIY TRNTKGGDAP AAGEDA

UniProtKB: Small ribosomal subunit protein eS25

Macromolecule #45: Small ribosomal subunit protein uS13

• Macromolecule: Name: Small ribosomal subunit protein uS13 / type: protein_or_peptide / ID: 45 / Details: Small ribosomal subunit protein uS13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.630598 KDa

Sequence

String:

MSLVIPEKFQ HILRVLNTNI DGRRKIAFAI TAIKGVGRRY AHVVLRKADI DLTKRAGELT EDEVERVITI MQNPRQYKIP DWFLNRQKD VKDGKYSQVL ANGLDNKLRE DLERLKKIRA HRGLRHFWGL RVRGQHTKTT GRRGRTVGVS KK

UniProtKB: Small ribosomal subunit protein uS13

Macromolecule #46: 40S ribosomal protein S29

• Macromolecule: Name: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 46 / Details: Small ribosomal subunit protein uS14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 6.690821 KDa

Sequence

String:

MGHQQLYWSH PRKFGQGSRS CRVCSNRHGL IRKYGLNMCR QCFRQYAKDI GFIKLD

UniProtKB: Small ribosomal subunit protein uS14

Macromolecule #47: Ubiquitin-40S ribosomal protein S27a

• Macromolecule: Name: Ubiquitin-40S ribosomal protein S27a / type: protein_or_peptide / ID: 47 / Details: Small ribosomal subunit protein eS31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.004041 KDa

Sequence

String:

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGAKKR KKKSYTTPK KNKHKRKKVK LAVLKYYKVD ENGKISRLRR ECPSDECGAG VFMASHFDRH YCGKCCLTYC FNKPEDK

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

Macromolecule #48: 40S ribosomal protein S12

• Macromolecule: Name: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 48 / Details: Small ribosomal subunit protein eS12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.538987 KDa

Sequence

String:

MAEEGIAAGG VMDVNTALQE VLKTALIHDG LARGIREAAK ALDKRQAHLC VLASNCDEPM YVKLVEALCA EHQINLIKVD DNKKLGEWV GLCKIDREGK PRKVVGCSCV VVKDYGKESQ AKDVIEEYFK CKK

UniProtKB: Small ribosomal subunit protein eS12

Macromolecule #49: 40S ribosomal protein S28

• Macromolecule: Name: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 49 / Details: Small ribosomal subunit protein eS28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 7.855052 KDa

Sequence

String:

MDTSRVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV REGDVLTLLE SEREARRLR

UniProtKB: Small ribosomal subunit protein eS28

Macromolecule #50: 40S ribosomal protein S17

• Macromolecule: Name: 40S ribosomal protein S17 / type: protein_or_peptide / ID: 50 / Details: Small ribosomal subunit protein eS17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.578156 KDa

Sequence

String:

MGRVRTKTVK KAARVIIEKY YTRLGNDFHT NKRVCEEIAI IPSKKLRNKI AGYVTHLMKR IQRGPVRGIS IKLQEEERER RDNYVPEVS ALDQEIIEVD PDTKEMLKLL DFGSLSNLQV TQPTVGMNFK TPRGPV

UniProtKB: Small ribosomal subunit protein eS17

Macromolecule #51: 40S ribosomal protein S20

• Macromolecule: Name: 40S ribosomal protein S20 / type: protein_or_peptide / ID: 51 / Details: Small ribosomal subunit protein uS10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.398763 KDa

Sequence

String:

MAFKDTGKTP VEPEVAIHRI RITLTSRNVK SLEKVCADLI RGAKEKNLKV KGPVRMPTKT LRITTRKTPC GEGSKTWDRF QMRIHKRLI DLHSPSEIVK QITSISIEPG VEVEVTIADA

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #52: Eukaryotic translation initiation factor 3 subunit D

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit D / type: protein_or_peptide / ID: 52 ; Details: Eukaryotic translation initiation factor 3 subunit D; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 64.060758 KDa

Sequence

String:

MAKFMTPVIQ DNPSGWGPCA VPEQFRDMPY QPFSKGDRLG KVADWTGATY QDKRYTNKYS SQFGGGSQYA YFHEEDESSF QLVDTARTQ KTAYQRNRMR FAQRNLRRDK DRRNMLQFNL QILPKSAKQK ERERIRLQKK FQKQFGVRQK WDQKSQKPRD S SVEVRSDW EVKEEMDFPQ LMKMRYLEVS EPQDIECCGA LEYYDKAFDR ITTRSEKPLR SIKRIFHTVT TTDDPVIRKL AK TQGNVFA TDAILATLMS CTRSVYSWDI VVQRVGSKLF FDKRDNSDFD LLTVSETANE PPQDEGNSFN SPRNLAMEAT YIN HNFSQQ CLRMGKERYN FPNPNPFVED DMDKNEIASV AYRYRRWKLG DDIDLIVRCE HDGVMTGANG EVSFINIKTL NEWD SRHCN GVDWRQKLDS QRGAVIATEL KNNSYKLARW TCCALLAGSE YLKLGYVSRY HVKDSSRHVI LGTQQFKPNE FASQI NLSV ENAWGILRCV IDICMKLEEG KYLILKDPNK QVIRVYSLPD GTFSSDEDEE EEEEEEEEEE EEET

UniProtKB: Eukaryotic translation initiation factor 3 subunit D

Macromolecule #55: Eukaryotic initiation factor 4A-I

• Macromolecule: Name: Eukaryotic initiation factor 4A-I / type: protein_or_peptide / ID: 55 / Details: Eukaryotic initiation factor 4A-I / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 46.207824 KDa
• Recombinant expression: Organism: Escherichia coli BL21(DE3) (bacteria)

Sequence

String:

MSASQDSRSR DNGPDGMEPE GVIESNWNEI VDSFDDMNLS ESLLRGIYAY GFEKPSAIQQ RAILPCIKGY DVIAQAQSGT GKTATFAIS ILQQIELDLK ATQALVLAPT RELAQQIQKV VMALGDYMGA SCHACIGGTN VRAEVQKLQM EAPHIIVGTP G RVFDMLNR RYLSPKYIKM FVLDEADEML SRGFKDQIYD IFQKLNSNTQ VVLLSATMPS DVLEVTKKFM RDPIRILVKK EE LTLEGIR QFYINVEREE WKLDTLCDLY ETLTITQAVI FINTRRKVDW LTEKMHARDF TVSAMHGDMD QKERDVIMRE FRS GSSRVL ITTDLLARGI DVQQVSLVIN YDLPTNRENY IHRIGRGGRF GRKGVAINMV TEEDKRTLRD IETFYNTSIE EMPL NVADL I

UniProtKB: Eukaryotic initiation factor 4A-I

Macromolecule #56: Eukaryotic translation initiation factor 4 gamma 1

• Macromolecule: Name: Eukaryotic translation initiation factor 4 gamma 1 / type: protein_or_peptide / ID: 56 / Details: Eukaryotic translation initiation factor 4 gamma / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 155.130141 KDa
• Recombinant expression: Organism: Baculovirus expression vector pFastBac1-HM

Sequence

String:

MSGARTASTP TPPQTGGGLE PQANGETPQV AVIVRPDDRS QGAIIADRPG LPGPEHSPSE SQPSSPSPTP SPSPVLEPGS EPNLAVLSI PGDTMTTIQM SVEESTPISR ETGEPYRLSP EPTPLAEPIL EVEVTLSKPV PESEFSSSPL QAPTPLASHT V EIHEPNGM VPSEDLEPEV ESSPELAPPP ACPSESPVPI APTAQPEELL NGAPSPPAVD LSPVSEPEEQ AKEVTASMAP PT IPSATPA TAPSATSPAQ EEEMEEEEEE EEGEAGEAGE AESEKGGEEL LPPESTPIPA NLSQNLEAAA ATQVAVSVPK RRR KIKELN KKEAVGDLLD AFKEANPAVP EVENQPPAGS NPGPESEGSG VPPRPEEADE TWDSKEDKIH NAENIQPGEQ KYEY KSDQW KPLNLEEKKR YDREFLLGFQ FIFASMQKPE GLPHISDVVL DKANKTPLRP LDPTRLQGIN CGPDFTPSFA NLGRT TLST RGPPRGGPGG ELPRGPQAGL GPRRSQQGPR KEPRKIIATV LMTEDIKLNK AEKAWKPSSK RTAADKDRGE EDADGS KTQ DLFRRVRSIL NKLTPQMFQQ LMKQVTQLAI DTEERLKGVI DLIFEKAISE PNFSVAYANM CRCLMALKVP TTEKPTV TV NFRKLLLNRC QKEFEKDKDD DEVFEKKQKE MDEAATAEER GRLKEELEEA RDIARRRSLG NIKFIGELFK LKMLTEAI M HDCVVKLLKN HDEESLECLC RLLTTIGKDL DFEKAKPRMD QYFNQMEKII KEKKTSSRIR FMLQDVLDLR GSNWVPRRG DQGPKTIDQI HKEAEMEEHR EHIKVQQLMA KGSDKRRGGP PGPPISRGLP LVDDGGWNTV PISKGSRPID TSRLTKITKP GSIDSNNQL FAPGGRLSWG KGSSGGSGAK PSDAASEAAR PATSTLNRFS ALQQAVPTES TDNRRVVQRS SLSRERGEKA G DRGDRLER SERGGDRGDR LDRARTPATK RSFSKEVEER SRERPSQPEG LRKAASLTED RDRGRDAVKR EAALPPVSPL KA ALSEEEL EKKSKAIIEE YLHLNDMKEA VQCVQELASP SLLFIFVRHG VESTLERSAI AREHMGQLLH QLLCAGHLST AQY YQGLYE ILELAEDMEI DIPHVWLYLA ELVTPILQEG GVPMGELFRE ITKPLRPLGK AASLLLEILG LLCKSMGPKK VGTL WREAG LSWKEFLPEG QDIGAFVAEQ KVEYTLGEES EAPGQRALPS EELNRQLEKL LKEGSSNQRV FDWIEANLSE QQIVS NTLV RALMTAVCYS AIIFETPLRV DVAVLKARAK LLQKYLCDEQ KELQALYALQ ALVVTLEQPP NLLRMFFDAL YDEDVV KED AFYSWESSKD PAEQQGKGVA LKSVTAFFKW LREAEEESDH N

UniProtKB: Eukaryotic translation initiation factor 4 gamma 1

Macromolecule #28: 18S rRNA

• Macromolecule: Name: 18S rRNA / type: rna / ID: 28 / Details: 18S rRNA / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 555.083875 KDa

Sequence

String:

UACCUGGUUG AUCCUGCCAG UAGCAU(A2M)UGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUG AGUACGCACG GCCGGU ACA GUGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGU(OMU)CC(PSU) U(OMU)GGUCGCUC GCUCCUACUU GGAUA ACUG UGGUA(A2M)UUCU AG(A2M)GCUAAUA (OMC)AUGCCGACG GGCGCUGACC CCCUUCGCGG GGGGGAUGCG UGCA UUUAU CAGUGGUGAC UCUAGAUAAC CUCGGGCCGA UCGCACGCCC CCCGUGGCGG CGACGACCCA UUCGAACGUC UGCCC UAUC AACUUUCGAU GGUAGUCGCC GUGCCUACCA UGGUGACCAC GGGUGACGGG GAAUCAGGGU UCGAUUCCGG AGAGGG AGC CUGAGAAACG GCUACCACAU CCAAGGAAGG CAGCAGGCGC GC(A2M)AAUUACC CACUCCCGAC CCGGGGA(OMG)G UAGUGA(OMC)GAA AAAUAACAAU ACAGGACUCU UUCGAGGCCC UGUAAUUGGA AUGAGUCCAC UUUAAAUCCU UUAACG AGG AUCCAUUGGA GGGCAAGUCU GG(PSU)GCCAGCA GCCGCGGUAA UUCCAGCUCC AAUA(OMG)CGUAU AUUAAAGUU GCUGCAGUU(A2M) AAAAGCUCGU AGUU(OMG)GAUCU UGGGAGCGGC CCCGCCCCUC UCGGCGGCCC GAAGCGUUUA CU UUGAAAA AA(5MU)UAGAGUG (PSU)(PSU)CAAAGCAG GCCCGAGCCG CCUGGAUACC GCAGCUAGGA AUAAUGGAAU A GGACCGCG GUUCUAUUUU GUUGGUUUUC GGAACUGAGG CCAUGAUUAA GAGGGACGGC CGGGGGCAUU CGUAUUGCGC CG CUAGAGG UGAAAUUCUU GGACCGGCGC AAGACGGACC AGAGCGAAAG CAUUUGCCAA GAAUGUUUUC AUUAAUCAAG A (A2M)CGAAAGU CGGAGGUUCG AAGACGAUCA GAUACCGUCG UAGUUCCGAC CA(PSU)AAACGAU GCCGACCGGC GAUG CGGCG GCGUUAUUCC CAUGACCCGC CGGGCAGCUU CCGGGAAACC AAAGUCUUUG GGUUCCGGGG GGAGUAUGGU UGCAA AGCU GAAACUUAAA GGAAUUGACG GAAGGGCACC AC(JMH)AGGAGUG GAGCCUGCGG CUUAAU(PSU)UGA C(C4J)CA ACACG GGAAACCUCA CCCGGCCCGG ACACGGACAG GAUUGACAGA UUGAUAGCUC UUUCUCGAUU CCGUGGGUGG UGGUG CAUG GCCGUUCUUA GUUGGUGGAG CGAUUUGUCU GGUUAAUUC(5MC) GAUAACGAAC GAGACUCUGG CAUGCUAACU AG UUACGCG ACCCCCGAGC GGUCGGCGUC CCCCAACUUC UUAGAGGGAC AAGUGGCGUU CAGCCACCCG AGAUUGAGCA AUA ACAGGU CUGUGAUGCC CUUAGAUGUC CGGGGCUGCA CGCGCGCUAC ACUGACUGGC UCAGCGUGUG CCUACCCUAC GCCG GCAGG CGCGGGUAAC CCGUUGAACC CCAUUCGUGA UGGGGAUCGG GGAUUGCAAU UAUUCCCCAU GAACGAGGAA UUCCC AGUA AGUGCGGGUC AUAAGCUUGC GUUGAUU(A2M)AG UCCCUGCCCU UUGUACACAC CG(OMC)CCGUCGC UACUACCG A UUGGAUGGUU UAGUGAGGCC CUCGGAUCGG CCCCGCCGGG GGCCCUGGCG GAGCGCUGAG AAGACGGUCG AACUUGACU AUCUAGAGGA AGUAAAAGUC G(UR3)A(6MZ)CAAGGU UUCCGUAGGU G(MA6)(MA6)CCUGCGG AAGGAUCAUU A

Macromolecule #53: tRNAiMet

• Macromolecule: Name: tRNAiMet / type: rna / ID: 53 / Details: Human initiator Met-tRNA-i / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.23151 KDa

Sequence

String:

AGCAGAGUGG CGCAGCGGAA GCGUGCUGGG CCCAUAACCC AGAGGUCGAU GGAUCGAAAC CAUCCUCUGC UACCA

GENBANK: GENBANK: K00328.1

Macromolecule #54: mRNA

• Macromolecule: Name: mRNA / type: rna / ID: 54 / Details: mRNA / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 67.626453 KDa

Sequence

String:

GGACAAGAGA GAGAGAGACU CCAACUCCAA GAGAGAGAGA GACAACUCCA AGAGAGAGAG AGACAAACCC UCGCUGAGCC GCAGUCAGA UCCUAGCGUC GAGUUGAUGC UGUCCGAUAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA A AAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAA

Macromolecule #57: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 57 / Number of copies: 2 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #58: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 58 / Number of copies: 89 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Grid: Model: UltrAuFoil R1.2/1.3 / Support film - Material: GRAPHENE OXIDE / Pretreatment - Type: GLOW DISCHARGE
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Software: Name: EPU
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 47.88 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.2 µm
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: OTHER
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 241389
• Initial angle assignment: Type: OTHER
• Final angle assignment: Type: OTHER