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- EMDB-16874: Structure of Candida albicans 80S ribosome in complex with mefloquine -
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基本情報
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タイトル | Structure of Candida albicans 80S ribosome in complex with mefloquine | |||||||||||||||
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![]() | Candida albicans / ribosome / mefloquine | |||||||||||||||
機能・相同性 | ![]() filamentous growth of a population of unicellular organisms in response to neutral pH / cellular response to neutral pH / filamentous growth of a population of unicellular organisms in response to biotic stimulus / filamentous growth of a population of unicellular organisms in response to starvation / negative regulation of cell integrity MAPK cascade / positive regulation of conjugation with cellular fusion / yeast-form cell wall / regulation of cytoplasmic translation / GCN2-mediated signaling / invasive growth in response to glucose limitation ...filamentous growth of a population of unicellular organisms in response to neutral pH / cellular response to neutral pH / filamentous growth of a population of unicellular organisms in response to biotic stimulus / filamentous growth of a population of unicellular organisms in response to starvation / negative regulation of cell integrity MAPK cascade / positive regulation of conjugation with cellular fusion / yeast-form cell wall / regulation of cytoplasmic translation / GCN2-mediated signaling / invasive growth in response to glucose limitation / hyphal cell wall / preribosome / negative regulation of p38MAPK cascade / filamentous growth / regulation of amino acid metabolic process / negative regulation of glucose mediated signaling pathway / positive regulation of translational fidelity / ribosome-associated ubiquitin-dependent protein catabolic process / GDP-dissociation inhibitor activity / pre-mRNA 5'-splice site binding / preribosome, small subunit precursor / nonfunctional rRNA decay / mRNA destabilization / signaling receptor activator activity / negative regulation of translational frameshifting / negative regulation of mRNA splicing, via spliceosome / translational elongation / G-protein alpha-subunit binding / 90S preribosome / ribosomal subunit export from nucleus / protein-RNA complex assembly / ribosomal small subunit export from nucleus / translation regulator activity / positive regulation of autophagy / DNA-(apurinic or apyrimidinic site) endonuclease activity / rescue of stalled ribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / cellular response to starvation / ribosomal large subunit biogenesis / protein kinase C binding / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / maintenance of translational fidelity / modification-dependent protein catabolic process / protein tag activity / rRNA processing / extracellular vesicle / ribosome biogenesis / large ribosomal subunit / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / cell adhesion / rRNA binding / protein ubiquitination / ribosome / structural constituent of ribosome / G protein-coupled receptor signaling pathway / translation / ribonucleoprotein complex / mRNA binding / ubiquitin protein ligase binding / nucleolus / cell surface / RNA binding / extracellular region / zinc ion binding / metal ion binding / nucleus / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||||||||
生物種 | ![]() | |||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.1 Å | |||||||||||||||
![]() | Kolosova O / Zgadzay Y / Stetsenko A / Guskov A / Yusupov M | |||||||||||||||
資金援助 | ![]() ![]()
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![]() | ![]() タイトル: Mechanism of read-through enhancement by aminoglycosides and mefloquine 著者: Kolosova O / Zgadzay Y / Yusupov M | |||||||||||||||
履歴 |
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構造の表示
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マップデータ | ![]() | 242.1 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 101.6 KB 101.6 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 13.9 KB | 表示 | ![]() |
画像 | ![]() | 149.3 KB | ||
Filedesc metadata | ![]() | 19.2 KB | ||
その他 | ![]() ![]() | 261.8 MB 261.8 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 8ogjMC ![]() 8cq7C ![]() 8cqwC ![]() 8creC ![]() 8oeqC ![]() 8oh6C ![]() 8oi5C ![]() 8oj3C M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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注釈 | Unfocused map | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.02 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-ハーフマップ: Half map A
ファイル | emd_16874_half_map_1.map | ||||||||||||
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注釈 | Half map A | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Half map B
ファイル | emd_16874_half_map_2.map | ||||||||||||
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注釈 | Half map B | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
+全体 : Candida albicans 80S ribosome in complex with mefloquine
+超分子 #1: Candida albicans 80S ribosome in complex with mefloquine
+分子 #1: 25S ribosomal RNA
+分子 #2: 5S ribosomal RNA
+分子 #3: 5.8S ribosomal RNA
+分子 #4: Mixture of endogenous E-tRNAs
+分子 #46: 18S ribosomal RNA
+分子 #5: 60S ribosomal protein L2-B
+分子 #6: 60S ribosomal protein L3
+分子 #7: 60S ribosomal protein L4-B
+分子 #8: 60S ribosomal protein L5
+分子 #9: 60S ribosomal protein L6
+分子 #10: 60S ribosomal protein L7-A
+分子 #11: 60S ribosomal protein L8
+分子 #12: 60S ribosomal protein L9-B
+分子 #13: 60S ribosomal protein L10
+分子 #14: 60S ribosomal protein L11-B
+分子 #15: 60S ribosomal protein L13
+分子 #16: 60S ribosomal protein L14-B
+分子 #17: 60S ribosomal protein L15-A
+分子 #18: Ribosomal protein L13
+分子 #19: Ribosomal protein L22
+分子 #20: 60S ribosomal protein L18-A
+分子 #21: 60S ribosomal protein L19-A
+分子 #22: 60S ribosomal protein L20
+分子 #23: 60S ribosomal protein L21-A
+分子 #24: 60S ribosomal protein L22-B
+分子 #25: 60S ribosomal protein L23-A
+分子 #26: 60S ribosomal protein L24-A
+分子 #27: 60S ribosomal protein L25
+分子 #28: Ribosomal protein L24
+分子 #29: 60S ribosomal protein L27
+分子 #30: 60S ribosomal protein L28
+分子 #31: 60S ribosomal protein L29
+分子 #32: 60S ribosomal protein L30
+分子 #33: 60S ribosomal protein L31-B
+分子 #34: 60S ribosomal protein L32
+分子 #35: 60S ribosomal protein L33-A
+分子 #36: 60S ribosomal protein L34-B
+分子 #37: Ribosomal protein L29
+分子 #38: 60S ribosomal protein L36
+分子 #39: 60S ribosomal protein L37-B
+分子 #40: 60S ribosomal protein L38
+分子 #41: 60S ribosomal protein L39
+分子 #42: 60S ribosomal protein L40-B
+分子 #43: 60S ribosomal protein L41
+分子 #44: 60S ribosomal protein L42-B
+分子 #45: 60S ribosomal protein L43-A
+分子 #47: 40S ribosomal protein S0
+分子 #48: 40S ribosomal protein S1
+分子 #49: Ribosomal protein S5
+分子 #50: Ribosomal protein S3
+分子 #51: 40S ribosomal protein S4
+分子 #52: Ribosomal protein S7
+分子 #53: 40S ribosomal protein S6
+分子 #54: 40S ribosomal protein S7
+分子 #55: 40S ribosomal protein S8
+分子 #56: Ribosomal protein S4
+分子 #57: 40S ribosomal protein S10-A
+分子 #58: 40S ribosomal protein S11A
+分子 #59: 40S ribosomal protein S12
+分子 #60: 40S ribosomal protein S13
+分子 #61: 40S ribosomal protein S14-A
+分子 #62: 40S ribosomal protein S15
+分子 #63: 40S ribosomal protein S16
+分子 #64: 40S ribosomal protein S17-B
+分子 #65: 40S ribosomal protein S18-B
+分子 #66: 40S ribosomal protein S19-A
+分子 #67: Ribosomal protein S10
+分子 #68: 40S ribosomal protein S21
+分子 #69: 40S ribosomal protein S22-A
+分子 #70: Ribosomal protein S23 (S12)
+分子 #71: 40S ribosomal protein S24
+分子 #72: 40S ribosomal protein S25
+分子 #73: 40S ribosomal protein S26
+分子 #74: 40S ribosomal protein S27
+分子 #75: 40S ribosomal protein S28-B
+分子 #76: 40S ribosomal protein S29A
+分子 #77: 40S ribosomal protein S30
+分子 #78: Ubiquitin-40S ribosomal protein S31 fusion protein
+分子 #79: Guanine nucleotide-binding protein subunit beta-like protein
+分子 #80: (11R,12S)- Mefloquine
+分子 #81: SPERMINE (FULLY PROTONATED FORM)
+分子 #82: MAGNESIUM ION
+分子 #83: ZINC ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.5 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TALOS ARCTICA |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 47.3 e/Å2 |
電子線 | 加速電圧: 200 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス(公称値): 16.0 µm / 最小 デフォーカス(公称値): 5.0 µm |
実験機器 | ![]() モデル: Talos Arctica / 画像提供: FEI Company |
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画像解析
-原子モデル構築 1
精密化 | 空間: REAL |
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得られたモデル | ![]() PDB-8ogj: |