This is a negative stain 3D reconstruction of a Rubisco assembly intermediate comprising eight copies of the large subunit RbcL and eight copies of the assembly chaperone RbcX2.
試料
試料: RbcL8-X8 Rubisco assembly intermediate containing 8 copies of the large Rubisco subunit RbcL and eight copies of the dimeric assembly chaperone RbcX2.
ジャーナル: Nature / 年: 2010 タイトル: Coupled chaperone action in folding and assembly of hexadecameric Rubisco. 著者: Cuimin Liu / Anna L Young / Amanda Starling-Windhof / Andreas Bracher / Sandra Saschenbrecker / Bharathi Vasudeva Rao / Karnam Vasudeva Rao / Otto Berninghausen / Thorsten Mielke / F Ulrich ...著者: Cuimin Liu / Anna L Young / Amanda Starling-Windhof / Andreas Bracher / Sandra Saschenbrecker / Bharathi Vasudeva Rao / Karnam Vasudeva Rao / Otto Berninghausen / Thorsten Mielke / F Ulrich Hartl / Roland Beckmann / Manajit Hayer-Hartl / 要旨: Form I Rubisco (ribulose 1,5-bisphosphate carboxylase/oxygenase), a complex of eight large (RbcL) and eight small (RbcS) subunits, catalyses the fixation of atmospheric CO(2) in photosynthesis. The ...Form I Rubisco (ribulose 1,5-bisphosphate carboxylase/oxygenase), a complex of eight large (RbcL) and eight small (RbcS) subunits, catalyses the fixation of atmospheric CO(2) in photosynthesis. The limited catalytic efficiency of Rubisco has sparked extensive efforts to re-engineer the enzyme with the goal of enhancing agricultural productivity. To facilitate such efforts we analysed the formation of cyanobacterial form I Rubisco by in vitro reconstitution and cryo-electron microscopy. We show that RbcL subunit folding by the GroEL/GroES chaperonin is tightly coupled with assembly mediated by the chaperone RbcX(2). RbcL monomers remain partially unstable and retain high affinity for GroEL until captured by RbcX(2). As revealed by the structure of a RbcL(8)-(RbcX(2))(8) assembly intermediate, RbcX(2) acts as a molecular staple in stabilizing the RbcL subunits as dimers and facilitates RbcL(8) core assembly. Finally, addition of RbcS results in RbcX(2) release and holoenzyme formation. Specific assembly chaperones may be required more generally in the formation of complex oligomeric structures when folding is closely coupled to assembly.
ダウンロード / ファイル: emd_1654.map.gz / 形式: CCP4 / 大きさ: 5.2 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
This is a negative stain 3D reconstruction of a Rubisco assembly intermediate comprising eight copies of the large subunit RbcL and eight copies of the assembly chaperone RbcX2.
名称: RbcL8-X8 Rubisco assembly intermediate containing 8 copies of the large Rubisco subunit RbcL and eight copies of the dimeric assembly chaperone RbcX2.
要素
試料: RbcL8-X8 Rubisco assembly intermediate containing 8 copies of the large Rubisco subunit RbcL and eight copies of the dimeric assembly chaperone RbcX2.
名称: RbcL8-X8 Rubisco assembly intermediate containing 8 copies of the large Rubisco subunit RbcL and eight copies of the dimeric assembly chaperone RbcX2. タイプ: sample / ID: 1000 / 集合状態: one RbcL-octamer binds to on RbcX2 octamer / Number unique components: 2