EMDB-16516: Cryo-EM structure NDUFS4 knockout complex I from Mus musculus hea...

Basic information

Entry

Database: EMDB / ID: EMD-16516

• Title: Cryo-EM structure NDUFS4 knockout complex I from Mus musculus heart (Class 2).
• Map data: globally sharpened consensus map

Sample

• Complex: Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
  • Protein or peptide: x 1 types
• Protein or peptide: x 41 types
• Ligand: x 12 types

• Keywords: NADH ubiquinone oxidoreductase / Complex I / OXIDOREDUCTASE

Function / homology

Function:

Mitochondrial protein import / mesenchymal stem cell proliferation / reproductive system development / Protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation / Complex I biogenesis / RHOG GTPase cycle / Respiratory electron transport / protein insertion into mitochondrial inner membrane / mesenchymal stem cell differentiation / circulatory system development / blastocyst hatching / Mitochondrial protein degradation / cellular response to oxygen levels / stem cell division / respiratory chain complex / response to light intensity / mitochondrial [2Fe-2S] assembly complex / iron-sulfur cluster assembly complex / mitochondrial large ribosomal subunit binding / gliogenesis / negative regulation of non-canonical NF-kappaB signal transduction / cardiac muscle tissue development / neural precursor cell proliferation / positive regulation of mitochondrial membrane potential / [2Fe-2S] cluster assembly / cellular response to glucocorticoid stimulus / oxygen sensor activity / response to hydroperoxide / iron-sulfur cluster assembly / positive regulation of ATP biosynthetic process / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I assembly / positive regulation of execution phase of apoptosis / mitochondrial electron transport, NADH to ubiquinone / NADH dehydrogenase activity / proton motive force-driven mitochondrial ATP synthesis / respiratory chain complex I / NADH dehydrogenase (ubiquinone) activity / quinone binding / cellular response to interferon-beta / ATP synthesis coupled electron transport / cellular response to retinoic acid / negative regulation of reactive oxygen species biosynthetic process / extrinsic apoptotic signaling pathway / neurogenesis / muscle contraction / Neutrophil degranulation / cerebellum development / reactive oxygen species metabolic process / DNA damage response, signal transduction by p53 class mediator / aerobic respiration / regulation of mitochondrial membrane potential / respiratory electron transport chain / kidney development / fatty acid metabolic process / mitochondrion organization / response to nicotine / response to cocaine / monooxygenase activity / electron transport chain / mitochondrial membrane / : / multicellular organism growth / response to hydrogen peroxide / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / circadian rhythm / positive regulation of protein catabolic process / NAD binding / fatty acid biosynthetic process / cellular senescence / FMN binding / nervous system development / myelin sheath / 4 iron, 4 sulfur cluster binding / gene expression / response to oxidative stress / in utero embryonic development / response to ethanol / electron transfer activity / response to hypoxia / mitochondrial inner membrane / nuclear speck / nuclear body / mitochondrial matrix / response to xenobiotic stimulus / negative regulation of DNA-templated transcription / neuronal cell body / dendrite / ubiquitin protein ligase binding / protein-containing complex binding / mitochondrion / nucleoplasm / ATP binding / metal ion binding / nucleus / cytoplasm

Domain/homology:

NmrA-like domain / NmrA-like family / Complex1_LYR-like / NADH-ubiquinone oxidoreductase 1 subunit C1 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH:ubiquinone oxidoreductase, ESSS subunit / ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1, NDUB1 / MNLL subunit / NADH-ubiquinone oxidoreductase flavoprotein 3 / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / Tim17/Tim22/Tim23/Pmp24 family / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, metazoa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, animal type / NADH dehydrogenase 1 beta subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUC2 / NADH:ubiquinone oxidoreductase, subunit NDUFB4 / NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2) / NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4) / NADH dehydrogenase 1, beta subcomplex, subunit 6 / NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit / : / NADH:ubiquinone oxidoreductase chain 4, N-terminal / NADH:ubiquinone oxidoreductase, chain 2 / NADH dehydrogenase subunit 2, C-terminal / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / : / : / NADH-ubiquinone oxidoreductase chain 4, amino terminus / NADH dehydrogenase subunit 2 C-terminus / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / : / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase MWFE subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / NADH-ubiquinone oxidoreductase NDSU1/NuoG-like, 4Fe-4S domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 / NADH:ubiquinone oxidoreductase subunit B14.5a (Complex I-B14.5a) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase 1 alpha subcomplex subunit 3 / Zinc finger, CHCC-type / Zinc-finger domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase subunit 5, C-terminal / NADH dehydrogenase subunit 5 C-terminus / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NADH:ubiquinone oxidoreductase, NDUFS5-15kDa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / GRIM-19 / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NDUFB9, LYR domain / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / NDUFA6, LYR domain / Deoxynucleoside kinase domain / Deoxynucleoside kinase / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal / : / SLBB domain / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / : / : / NADH-quinone oxidoreductase subunit 3, ferredoxin-like domain / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ / NADH-quinone oxidoreductase, chain M/4 / NADH-ubiquinone oxidoreductase chain 4L/K / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus / NAD(P)H-quinone oxidoreductase subunit D/H / NADH-quinone oxidoreductase, chain 5-like / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / NADH-ubiquinone oxidoreductase chain 4L/Mnh complex subunit C1-like / NADH-ubiquinone/plastoquinone oxidoreductase chain 4L / Respiratory chain NADH dehydrogenase 49 Kd subunit signature. / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. / NADH-quinone oxidoreductase, subunit D / Respiratory-chain NADH dehydrogenase, 49 Kd subunit / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. / NADH ubiquinone oxidoreductase, F subunit / NADH dehydrogenase, subunit C

Component:

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial / NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 subunit C2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / Acyl carrier protein, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

• Biological species: Mus musculus (house mouse)
• Method: single particle reconstruction / cryo EM / Resolution: 3.2 Å
• Authors: Yin Z / Bridges HR / Agip ANA / Hirst J

Funding support

United Kingdom, 3 items

Organization	Grant number	Country
Medical Research Council (MRC, United Kingdom)	MC_U105663141	United Kingdom
Medical Research Council (MRC, United Kingdom)	MC_UU_00015/2	United Kingdom
Medical Research Council (MRC, United Kingdom)	MC_UU_00028/1	United Kingdom

• Citation: Journal: EMBO J / Year: 2024; Title: Structural insights into respiratory complex I deficiency and assembly from the mitochondrial disease-related ndufs4 mouse.; Authors: Zhan Yin / Ahmed-Noor A Agip / Hannah R Bridges / Judy Hirst / ; Abstract: Respiratory complex I (NADH:ubiquinone oxidoreductase) is essential for cellular energy production and NAD homeostasis. Complex I mutations cause neuromuscular, mitochondrial diseases, such as Leigh Syndrome, but their molecular-level consequences remain poorly understood. Here, we use a popular complex I-linked mitochondrial disease model, the ndufs4 mouse, to define the structural, biochemical, and functional consequences of the absence of subunit NDUFS4. Cryo-EM analyses of the complex I from ndufs4 mouse hearts revealed a loose association of the NADH-dehydrogenase module, and discrete classes containing either assembly factor NDUFAF2 or subunit NDUFS6. Subunit NDUFA12, which replaces its paralogue NDUFAF2 in mature complex I, is absent from all classes, compounding the deletion of NDUFS4 and preventing maturation of an NDUFS4-free enzyme. We propose that NDUFAF2 recruits the NADH-dehydrogenase module during assembly of the complex. Taken together, the findings provide new molecular-level understanding of the ndufs4 mouse model and complex I-linked mitochondrial disease.

History

Deposition	Jan 24, 2023	-
Header (metadata) release	Dec 20, 2023	-
Map release	Dec 20, 2023	-
Update	Jan 31, 2024	-
Current status	Jan 31, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_16516.map.gz / Format: CCP4 / Size: 347.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: globally sharpened consensus map
• Voxel size: X=Y=Z: 1.352 Å

Density

Contour Level	By AUTHOR: 1.9
Minimum - Maximum	-4.972478 - 13.938658
Average (Standard dev.)	0.004861701 (±0.42243558)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 450 450 450 Spacing 450 450 450
Cell	A=B=C: 608.4 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_16516_msk_1.map

Half map: half map 1

• File: emd_16516_half_map_1.map
• Annotation: half map 1

Half map: half map 2

• File: emd_16516_half_map_2.map
• Annotation: half map 2

Sample components

Entire : Very long-chain specific acyl-CoA dehydrogenase, mitochondrial

• Entire: Name: Very long-chain specific acyl-CoA dehydrogenase, mitochondrial

Components

• Complex: Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 3
• Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
• Protein or peptide: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
• Protein or peptide: NADH-ubiquinone oxidoreductase chain 1
• Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
• Protein or peptide: NADH-ubiquinone oxidoreductase chain 6
• Protein or peptide: NADH-ubiquinone oxidoreductase chain 4L
• Protein or peptide: NADH-ubiquinone oxidoreductase chain 5
• Protein or peptide: NADH-ubiquinone oxidoreductase chain 4
• Protein or peptide: NADH-ubiquinone oxidoreductase chain 2
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
• Protein or peptide: Acyl carrier protein, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
• Protein or peptide: MCG5603
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 subunit C2
• Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
• Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
• Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial
• Ligand: IRON/SULFUR CLUSTER
• Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
• Ligand: FE2/S2 (INORGANIC) CLUSTER
• Ligand: FLAVIN MONONUCLEOTIDE
• Ligand: Ubiquinone-9
• Ligand: 1,2-Distearoyl-sn-glycerophosphoethanolamine
• Ligand: CARDIOLIPIN
• Ligand: DODECYL-BETA-D-MALTOSIDE
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
• Ligand: ZINC ION
• Ligand: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate

Supramolecule #1: Very long-chain specific acyl-CoA dehydrogenase, mitochondrial

• Supramolecule: Name: Very long-chain specific acyl-CoA dehydrogenase, mitochondrial; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
• Source (natural): Organism: Mus musculus (house mouse)

Macromolecule #1: NADH-ubiquinone oxidoreductase chain 3

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 3 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 13.251785 KDa

Sequence

String:

(FME)NLYTVIFIN ILLSLTLILV AFWLPQMNLY SEKANPYECG FDPTSSARLP FSMKFFLVAI TFLLFDLEIA LLLPLP WAI QTIKTSTMMI MAFILVTILS LGLAYEWTQK GLEWTE

UniProtKB: NADH-ubiquinone oxidoreductase chain 3

Macromolecule #2: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial; type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 24.715912 KDa

Sequence

String:

MAALAAPGLL SVRILGLRTA QVQLRRVHQS VATEGPSPSP SPSLSSTQSA VSKAGAGAVV PKLSHLPRSR AEYVVTKLDD LINWARRSS LWPMTFGLAC CAVEMMHMAA PRYDMDRFGV VFRASPRQAD VMIVAGTLTN KMAPALRKVY DQMPEPRYVV S MGSCANGG GYYHYSYSVV RGCDRIVPVD IYVPGCPPTA EALLYGILQL QRKIKREQKL KIWYRR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

Macromolecule #3: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial; type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 30.191307 KDa

Sequence

String:

MAAAAARVWC RGLLGAASVG RGAGRPSVLW QHVRRESAAA DKRPTVRPRS DVTHKQLSAF GEYVAEILPK YVQQVQVSCL DELEICIHP DGVIPTLTFL RDHTNAQFKS LADLTAVDVP TRQNRFEIVY NLLSLRFNSR IRVKTYADEL TPIDSIVSVH I AANWYERE VWDMFGVFFF NHPDLRRILT DYGFEGHPFR KDFPLTGYVE LRYDDEVKRV VAEPVELAQE FRKFDLNSPW EA FPAYRQP PESLKLEAGD KKPETK

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

Macromolecule #4: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial; type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 52.720602 KDa

Sequence

String:

MAALRALRCL RGVGAPVLRP GSGIRLPSQP SRGARQWQPD IEWAEQFSGA VMYPSKETAH WKPPPWNDVD ILKEKAVTNM TLNFGPQHP AAHGVLRLVL ELSGEMVRKC DPHIGLLH(2MR)G TEKLIEYKTY LQALPYFDRL DYVSMMCNEQ AYSIAVE KL LNIQPPPRAQ WIRVLFGEIT RILNHIMAVT THALDIGAMT PFFWMFEERE KMFEFYERVS GARMHAAYIR PGGVHQDL P LGLLDDIYEF SKNFSLRIDE VEEMLTNNRI WRNRTVDIGV VTAEDALNYG FSGVMLRGSG IQWDLRKTQP YDVYDQVEF DVPIGSRGDC YDRYLCRVEE MRQSLRIIEQ CLNKMPPGEI KVDDAKVSPP KRAEMKTSME SLIHHFKLYT EGYQVPPGAT YTAIEAPKG EFGVYLVSDG SSRPYRCKIK APGFAHLAGL DKMSKGHMLA DVVAIIGTQD IVFGEIDR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

Macromolecule #5: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 27.077047 KDa

Sequence

String:

MFSLALRARA TGLAAQWGRH ARNLHKTAVH NGAGGALFVH RDTPENNPDT PFDFTPENYK RIEAIVKNYP EGHQAAAVLP VLDLAQRQN GWLPISAMNK VAEVLQVPPM RVYEVATFYT MYNRKPVGKY HIQVCTTTPC MLRDSDSILE TLQRKLGIKV G ETTPDKLF TLIEVECLGA CVNAPMVQIN DNYYEDLTPK DIEEIIDELK AGKVPKPGPR SGRFCCEPAG GLTSLTEPPK GP GFGVQ

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

Macromolecule #6: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial; type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 50.904152 KDa

Sequence

String:

MLAARHFLGG LVPVRVSVRF SSGTTAPKKT SFGSLKDEDR IFTNLYGRHD WRLKGALRRG DWYKTKEILL KGPDWILGEM KTSGLRGRG GAGFPTGLKW SFMNKPSDGR PKYLVVNADE GEPGTCKDRE IMRHDPHKLV EGCLVGGRAM GARAAYIYIR G EFYNEASN LQVAIREAYE AGLIGKNACG SDYDFDVFVV RGAGAYICGE ETALIESIEG KQGKPRLKPP FPADVGVFGC PT TVANVET VAVSPTICRR GGTWFAGFGR ERNSGTKLFN ISGHVNHPCT VEEEMSVPLK ELIEKHAGGV TGGWDNLLAV IPG GSSTPL IPKSVCETVL MDFDALVQAQ TGLGTAAVIV MDRSTDIVKA IARLIEFYKH ESCGQCTPCR EGVDWMNKVM ARFV KGDAR PAEIDSLWEI SKQIEGHTIC ALGDGAAWPV QGLIRHFRPE LEDRMQRFAQ QHRAWQAAS

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

Macromolecule #7: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

• Macromolecule: Name: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 79.866688 KDa

Sequence

String:

MLRIPIKRAL IGLSNSPKGY VRTTGTAASN LIEVFVDGQS VMVEPGTTVL QACEKVGMQI PRFCYHERLS VAGNCRMCLV EIEKAPKVV AACAMPVMKG WNILTNSEKS KKAREGVMEF LLANHPLDCP ICDQGGECDL QDQSMMFGSD RSRFLEGKRA V EDKNIGPL VKTIMTRCIQ CTRCIRFASE IAGVDDLGTT GRGNDMQVGT YIEKMFMSEL SGNVIDICPV GALTSKPYAF TA RPWETRK TESIDVMDAV GSNIVVSTRT GEVMRILPRM HEDINEEWIS DKTRFAYDGL KRQRLTEPMV RNEKGLLTYT SWE DALSRV AGMLQNFEGN AVAAIAGGLV DAEALVALKD LLNKVDSDNL CTEEIFPTEG AGTDLRSNYL LNTTIAGVEE ADVV LLVGT NPRFEAPLFN ARIRKSWLHN DLKVALIGSP VDLTYRYDHL GDSPKILQDI ASGRHSFCEV LKDAKKPMVV LGSSA LQRD DGAAILVAVS NMVQKIRVTT GVAAEWKVMN ILHRIASQVA ALDLGYKPGV EAIRKNPPKM LFLLGADGGC ITRQDL PKD CFIVYQGHHG DVGAPMADVI LPGAAYTEKS ATYVNTEGRA QQTKVAVTPP GLAREDWKII RALSEIAGIT LPYDTLD QV RNRLEEVSPN LVRYDDIEET NYFQQASELA KLVNQEVLAD PLVPPQLTIK DFYMTDSISR ASQTMAKCVK AVTEGAQA V EEPSIC

UniProtKB: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Macromolecule #8: NADH-ubiquinone oxidoreductase chain 1

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 1 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 36.105027 KDa

Sequence

String:

(FME)FFINILTLL VPILIAMAFL TLVERKILGY MQLRKGPNIV GPYGILQPFA DAMKLFMKEP MRPLTTSMSL FIIAPT LSL TLALSLWVPL PMPHPLINLN LGILFILATS SLSVYSILWS GWASNSKYSL FGALRAVAQT ISYEVTMAII LLSVLLM NG SYSLQTLITT QEHMWLLLPA WPMAMMWFIS TLAETNRAPF DLTEGESELV SGFNVEYAAG PFALFFMAEY TNIILMNA L TTIIFLGPLY YINLPELYST NFMMEALLLS STFLWIRASY PRFRYDQLMH LLWKNFLPLT LALCMWHISL PIFTAGVPP YM

UniProtKB: NADH-ubiquinone oxidoreductase chain 1

Macromolecule #9: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial; type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 24.068355 KDa

Sequence

String:

MYRLSSSMLP RALAQAMRTG HLNGQSLHSS AVAATYKYVN KKEQESEVDM KSATDNAARI LMWTELIRGL GMTLSYLFRE PATINYPFE KGPLSPRFRG EHALRRYPSG EERCIACKLC EAICPAQAIT IEAEPRADGS RRTTRYDIDM TKCIYCGFCQ E ACPVDAIV EGPNFEFSTE THEELLYNKE KLLNNGDKWE AEIAANIQAD YLYR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Macromolecule #10: NADH-ubiquinone oxidoreductase chain 6

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 6 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 18.6561 KDa

Sequence

String:

(FME)NNYIFVLSS LFLVGCLGLA LKPSPIYGGL GLIVSGFVGC LMVLGFGGSF LGLMVFLIYL GGMLVVFGYT TAMATE EYP ETWGSNWLIL GFLVLGVIME VFLICVLNYY DEVGVINLDG LGDWLMYEVD DVGVMLEGGI GVAAMYSCAT WMMVVAG WS LFAGIFIIIE ITRD

UniProtKB: NADH-ubiquinone oxidoreductase chain 6

Macromolecule #11: NADH-ubiquinone oxidoreductase chain 4L

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 4L / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 10.637629 KDa

Sequence

String:

(FME)PSTFFNLTM AFSLSLLGTL MFRSHLMSTL LCLEGMVLSL FIMTSVTSLN SNSMSSMPIP ITILVFAACE AAVGLA LLV KVSNTYGTDY VQNLNLLQC

UniProtKB: NADH-ubiquinone oxidoreductase chain 4L

Macromolecule #12: NADH-ubiquinone oxidoreductase chain 5

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 5 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 68.547297 KDa

Sequence

String:

(FME)NIFTTSILL IFILLLSPIL ISMSNLIKHI NFPLYTTTSI KFSFIISLLP LLMFFHNNME YMITTWHWVT MNSMEL KMS FKTDFFSILF TSVALFVTWS IMQFSSWYMH SDPNINRFIK YLTLFLITML ILTSANNMFQ LFIGWEGVGI MSFLLIG WW YGRTDANTAA LQAILYNRIG DIGFILAMVW FSLNMNSWEL QQIMFSNNND NLIPLMGLLI AATGKSAQFG LHPWLPSA M EGPTPVSALL HSSTMVVAGI FLLVRFHPLT TNNNFILTTM LCLGALTTLF TAICALTQND IKKIIAFSTS SQLGLMMVT LGMNQPHLAF LHICTHAFFK AMLFMCSGSI IHSLADEQDI RKMGNITKIM PFTSSCLVIG SLALTGMPFL TGFYSKDLII EAINTCNTN AWALLITLIA TSMTAMYSMR IIYFVTMTKP RFPPLISINE NDPDLMNPIK RLAFGSIFAG FVISYNIPPT S IPVLTMPW FLKTTALIIS VLGFLIALEL NNLTMKLSMN KANPYSSFST LLGFFPSIIH RITPMKSLNL SLKTSLTLLD LI WLEKTIP KSTSTLHTNM TTLTTNQKGL IKLYFMSFLI NIILIIILYS INLE

UniProtKB: NADH-ubiquinone oxidoreductase chain 5

Macromolecule #13: NADH-ubiquinone oxidoreductase chain 4

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 4 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 51.943547 KDa

Sequence

String:

(FME)LKIILPSLM LLPLTWLSSP KKTWTNVTSY SFLISLTSLT LLWQTDENYK NFSNMFSSDP LSTPLIILTA WLLPLM LMA SQNHLKKDNN VLQKLYISML ISLQILLIMT FSATELIMFY ILFEATLIPT LIIITRWGNQ TERLNAGIYF LFYTLIG SI PLLIALILIQ NHVGTLNLMI LSFTTHTLDA SWSNNLLWLA CMMAFLIKMP LYGVHLWLPK AHVEAPIAGS MILAAILL K LGSYGMIRIS IILDPLTKYM AYPFILLSLW GMIMTSSICL RQTDLKSLIA YSSVSHMALV IASIMIQTPW SFMGATMLM IAHGLTSSLL FCLANSNYER IHSRTMIMAR GLQMVFPLMA TWWLMASLAN LALPPSINLM GELFITMSLF SWSNFTIILM GINIIITGM YSMYMIITTQ RGKLTNHMIN LQPSHTRELT LMALHMIPLI LLTTSPKLIT GLTM

UniProtKB: NADH-ubiquinone oxidoreductase chain 4

Macromolecule #14: NADH-ubiquinone oxidoreductase chain 2

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 2 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 38.80023 KDa

Sequence

String:

(FME)NPITLAIIY FTIFLGPVIT MSSTNLMLMW VGLEFSLLAI IPMLINKKNP RSTEAATKYF VTQATASMII LLAIVL NYK QLGTWMFQQQ TNGLILNMTL MALSMKLGLA PFHFWLPEVT QGIPLHMGLI LLTWQKIAPL SILIQIYPLL NSTIILM LA ITSIFMGAWG GLNQTQMRKI MAYSSIAHMG WMLAILPYNP SLTLLNLMIY IILTAPMFMA LMLNNSMTIN SISLLWNK T PAMLTMISLM LLSLGGLPPL TGFLPKWIII TELMKNNCLI MATLMAMMAL LNLFFYTRLI YSTSLTMFPT NNNSKMMTH QTKTKPNLMF STLAIMSTMT LPLAPQLIT

UniProtKB: NADH-ubiquinone oxidoreductase chain 2

Macromolecule #15: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mi...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 40.657375 KDa

Sequence

String:

MALRLLRLVP ASAPARGLAA GAQRVGRIHT SVHCKLRYGL LAAILGDKTT KKLHEYSRVI TVDGNICSGK NKLAKEIAQQ LGMKHYPEA GIQYSSTTTG DGRPLDIEFS GSCSLEKFYD DPKSNDGNSY RLQSWLYASR LLQYADALEH LLSTGQGVVL E RSIYSDFV FLEAMYNQGY IRKQCVDHYN EIKRLTLPEY LPPHAVIYID VPVPEVQSRI QKKGDPHEMK VTSAYLQDIE NA YKKTFLP KMSEMCEVLV YDSWEAEDPT KVVEDIEYLK YNKGPWLKQD DWTFHYLRML VQDKTEVLNY TTIPVYLPEI TIG AHQGSR IYNSFRELPG RKYAPGYNAE VGDKWIWLK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial

Macromolecule #16: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mit...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial; type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 42.588129 KDa

Sequence

String:

MAAAVRFRVV RALPMSRPAI TAAATSVFCG SSHRQLHHAV IPHGKGGRSS VSGVVATVFG ATGFLGRYVV NHLGRMGSQV IIPYRCDVY DIMHLRLMGD LGQLTFLEWD ARDKDSIRKA VQHSNVVINL IGREWETRNF DFEDVFVNIP RAIAQASKEA G VERFIHVS HLNASMKSSS KSLRSKAVGE KEVRSVFPEA IIIRPSDIFG REDRFLNHFA NYRWFLAVPL VSLGFKTVKQ PV YVADVSK GIVNATKDPD AVGKTFAFTG PNRYLLFHLV KYIFGMTHRT FIPYPLPLFV YSWIGKLFGL SPFEPWTTKD KVE RIHISD VMPTDLPGLE DLGVQPTPLE LKSIEVLRRH RTYRWLSSEI EETKPAKTVN Y

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial

Macromolecule #17: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial; type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 13.041828 KDa

Sequence

String:

MAAVLTFRRL LTLPRAARGF GVQVSPSGEK ITHTGQVYDE KDYRRVRFVD RQKEVNENFA IDLIAQQPVN EVEHRIIACD GGGGALGHP KVYINLDKET KTGTCGYCGL QFKQHHH

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

Macromolecule #18: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 10.932675 KDa

Sequence

String:

MAAAAASRAV GAKLGLREIR VHLCQRSPGS QGVRDFIVQR YVELKKAHPN LPILIRECSE VQPKLWARYA FGQEKTVSLN NLSADEVTR AMQNVLSGKA

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

Macromolecule #19: Acyl carrier protein, mitochondrial

• Macromolecule: Name: Acyl carrier protein, mitochondrial / type: protein_or_peptide / ID: 19 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 17.390289 KDa

Sequence

String:

MASRVLCACV RRLPAAFAPL PRLPTLALAR PLSTTLCPEG IRRRPGALQS ALALAQVPGT VTHLCRQYSD APPLTLDGIK DRVLYVLKL YDKIDPEKLS VNSHFMKDLG LDSLDQVEII MAMEDEFGFE IPDIDAEKLM CPQEIVDYIA DKKDVYE

UniProtKB: Acyl carrier protein, mitochondrial

Macromolecule #20: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5; type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 13.380719 KDa

Sequence

String:

MAGLLKKTTG LVGLAVCDTP HERLTILYTK TLDILKHFPK HAAYRKYTEQ ITNEKLDMVK AEPDVKKLEA LLQGGEVEEV ILQAEKELS LARKMLKWKP WEPLVEEPPA NQWKWPI

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

Macromolecule #21: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6; type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.311858 KDa

Sequence

String:

MAAAATGLRQ AAAAAASTSV KPIFSRDLNE AKRRVRELYR AWYREVPNTV HLMQLDITVK QGRDKVREMF MKNAHVTDPR VVDLLVIKG KMELQETIKV WKQRTHVMRF FHETETPRPK DFLSKFYMGH DP

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

Macromolecule #22: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 20.025127 KDa

Sequence

String:

MPGIVELPTL EELKVEEVKV SSAVLKAAAH HYGAQCDKTN KEFMLCRWEE KDPRRCLKEG KLVNGCALNF FRQIKSHCAE PFTEYWTCL DYSNMQLFRH CRQQQAKFDQ CVLDKLGWVR PDLGQLSKVT KVKTDRPLPE NPYHSRARPE PNPVIEGDLK P AKHGTRFF FWTV

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

Macromolecule #23: MCG5603

• Macromolecule: Name: MCG5603 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.130416 KDa

Sequence

String:

MAMVKRFFES YHEVPDGTQC HRKTYITTAL GGICGIIGSA YSVSLNPADS TLEAVARVGR YTFTAAAIGA MFGLTTCVSA QVREKPDDP LNYFIGGCAG GLTLGARTHS YGTAAMGCVY MGTAAALFKI GKLEGWELFP TPKV

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11

Macromolecule #24: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13; type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 16.881588 KDa

Sequence

String:

MAASKVKQDM PPPGGYGPID YKRNLPRRGL SGYSMFAVGI GALIFGYWRM MRWNQERRRL LIEDLEARIA LMPLFQAEKD RRTLQILRE NLEEEAIIMK DVPNWKVGES VFHTTRWVPP LIGEMYGLRT KEEMSNANFG FTWYT

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

Macromolecule #25: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 8.149524 KDa

Sequence

String:

MWFEILPGLA IMGVCLVIPG VSTAYIHKFT NGGKEKRVAR VQYQWYLMER DRRISGVNRY YVSKGLENID

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

Macromolecule #26: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3; type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 9.338867 KDa

Sequence

String:

MAGRISAFLK NAWAKEPVLV VSFSVWGLAI IMPMISPYTK YASMINKATP YNYPVPVRDD GNMPDVPSHP QDPLGPSLDW LKNL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

Macromolecule #27: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial; type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 8.636023 KDa

Sequence

String:

MAPSVVLRSF SRLLAPARLP SCSSTRSKFY VREPVNAKPN WLAVGLSVGA SVFMWIYLIQ THNEDVLEYK RRNGLE

UniProtKB: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial

Macromolecule #28: NADH dehydrogenase [ubiquinone] 1 subunit C2

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 subunit C2 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 14.185692 KDa

Sequence

String:

MMNGRPGHEP LKFLPDEARS LPPPKLNDPR LVYMGLLGYC TGLMDNMLRM RPVMRAGLHR QLLFVTSFVF AGYFYLKRQN YLYAVKDHD MFGYIKLHPE DFPEKEKKTY AEILEPFHPV R

UniProtKB: NADH dehydrogenase [ubiquinone] 1 subunit C2

Macromolecule #29: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 12.675772 KDa

Sequence

String:

MPFLDIQKKL GISLDRHFMF LSAEQPYKNA ARCHAFEKEW IECAHGIGGT RAKKECKIEF DDFEECLLRY KTMRRMHDIK KQREKLMKE GKYTPPPHHS GREEPRP

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

Macromolecule #30: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1; type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 6.965109 KDa

Sequence

String:

MTLFQLLREH WVHILVPAGF VFGCYLDRKD DEKLTAFRNK SMLFQRELRP NEEVTWK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1

Macromolecule #31: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mit...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial; type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 17.463727 KDa

Sequence

String:

MAARLLSLYG RCLSAAGAMR GLPAARVRWE SSRAVIAPSG VEKKRQREPT MQWQEDPEPE DENVYAKNPD FHGYDSDPVV DVWNMRAVF FFGFSIVLVF GTTFVAYVPD YRMQEWARRE AERLVKYREV NGLPIMESNY FDPSKIQLPE DD

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial

Macromolecule #32: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mito...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial; type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 21.742197 KDa

Sequence

String:

MAAMSLLQRA SVSALTALSC RRAGPRLGVG SFLTRSFPKT VAPVRHSGDH GKRLFVVKPS LYYDARFLRL MKFYLMLTGI PVIIGITLV NIFIGEAELA EIPEGYIPEH WEYYKHPISR WIARNFYDGP EKNYEKTLAI LQIESEKAEL RLKEQEVRRL M RARGDGPW YQFPTPEKEF IDHSPKATPD N

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial

Macromolecule #33: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6; type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.450925 KDa

Sequence

String:

(SAC)GYTPDEKLR LQQLRELRRR WLKDQELSPR EPVLPPRRMW PLERFWDNFL RDGAVWKNMV FKAYRSSLFA VSHVLI PMW FVHYYVKYHM ATKPYTIVSS KPRIFPGDTI LETGEVIPPM RDFPDQHH

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6

Macromolecule #34: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mito...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial; type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 11.982437 KDa

Sequence

String:

MSALTRLVPF GRVGGRLLRG CRARAAGDSG VRHAGGGVHI QPRYREFPQL TRSQVIQGEF LSSLMWFWIL WRFWHDSDAV LGHFSYPDP SQWTDEELGI PPDDED

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial

Macromolecule #35: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3; type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 11.71424 KDa

Sequence

String:

MAAGHGHEHG HEHGHGHGKM ELPDYRQWKI EGTPLETVQK KLAARGLRDP WARNEAWRYM GGFAGNITFP SVILKGFKWG FAAFVVALG AEYFLDSQNG DKKHH

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3

Macromolecule #36: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mito...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 21.903828 KDa

Sequence

String:

MAAARAAALG VRWLQRTTRG VVPLEARRAF HMTKDMLPGS YPRTPEERAA AAKKYNMRVE DYEPYPDDGM GYGDYPMLPN RSQHERDPW YQWDHSELRM NWGEPIHWDL DMYIRNRVDT SPTPVSWDVM CKHLFGFVAF MVFMFWVGHV FPSYQPVGPK Q YPYNNLYL ERGGDPTKEP EPVVHYDI

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial

Macromolecule #37: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4; type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 15.105287 KDa

Sequence

String:

MSGSKYKPAP LATLPSTLDP AEYDVSPETR RAQVERLSIR ARLKREYLLQ YNDPKRVSHI EDPALIRWTY ARSANIYPNF RPTPKNSLL GAVAGFGPLI FWYYVFKTDR DRKERLIQEG KLDRKFNISY

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

Macromolecule #38: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 22.020123 KDa

Sequence

String:

MAFCAPPAYL THQQKVLRLY KRALRHLESW CIHRDKYRYF ACLMRARFEE HKNEKDMMRA TQLLREAEEE FWQNQHPQPY IFPDSPGGT SFERYECYKV PEWCLDYWHP SEKAMYPDYF SKREQWKKLR MESWDREVKQ LQEETSPDGI MTEALPPARR E GDLPPLWW HIVTRPRERP T

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

Macromolecule #39: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7; type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 16.360804 KDa

Sequence

String:

MGAHLTRRYL WDASVEPDPE KIPSFPPDLG FPERKERVMV ATQQEMMDAQ LTLQQRDYCA HYLIRLLKCK RDSFPNFLAC KHEQHDWDY CEHLDYVKRM KEFERERRLL QRKKRRALKE ARVAQGQGEG EVGPEVAL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

Macromolecule #40: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10; type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 21.054832 KDa

Sequence

String:

MPDSWDKDVY PEPPSRTPAP SPQTSLPNPI TYLTKAYDLV VDWPVTLVRE FIERQHAKNR TYYYHRQYRR VPDITECKEG DVLCIYEAE MQWRRDFKVD QEIMNIIQER LKACQQREGE NYQQNCAKEL EQFTKVTKAY QDRYLDLGAY YSARKCLAKQ K QRMLEERK AARQEAAA

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

Macromolecule #41: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7; type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 12.595592 KDa

Sequence

String:

MASATRVIQK LRNWASGQDL QAKLQLRYQE IAKRTQPPPK LPVGPSHKLS NNYYCTRDGR REVVPPSIIM SSQKALVSGK AAESSAMAA TEKKAVTPAP PMKRWELSKD QPYL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

Macromolecule #42: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial; type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 11.833504 KDa

Sequence

String:

MAVSLLLRGG RIRALKAVLL EARVFPGELV SVVRLSTESE KSAKEKELHP KTQSVLKEPE PTDTTTYKNL QHHDYNTYTF LDLNLDLSK FRLPQPSSGR ESPRH

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial

Macromolecule #43: IRON/SULFUR CLUSTER

• Macromolecule: Name: IRON/SULFUR CLUSTER / type: ligand / ID: 43 / Number of copies: 6 / Formula: SF4
• Molecular weight: Theoretical: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

Macromolecule #44: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE

• Macromolecule: Name: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / type: ligand / ID: 44 / Number of copies: 2 / Formula: PC1
• Molecular weight: Theoretical: 790.145 Da

Chemical component information

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM

Macromolecule #45: FE2/S2 (INORGANIC) CLUSTER

• Macromolecule: Name: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 45 / Number of copies: 2 / Formula: FES
• Molecular weight: Theoretical: 175.82 Da

Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

Macromolecule #46: FLAVIN MONONUCLEOTIDE

• Macromolecule: Name: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 46 / Number of copies: 1 / Formula: FMN
• Molecular weight: Theoretical: 456.344 Da

Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

Macromolecule #47: Ubiquinone-9

• Macromolecule: Name: Ubiquinone-9 / type: ligand / ID: 47 / Number of copies: 1 / Formula: UQ9
• Molecular weight: Theoretical: 795.226 Da

Chemical component information

ChemComp-UQ9:
Ubiquinone-9

Macromolecule #48: 1,2-Distearoyl-sn-glycerophosphoethanolamine

• Macromolecule: Name: 1,2-Distearoyl-sn-glycerophosphoethanolamine / type: ligand / ID: 48 / Number of copies: 7 / Formula: 3PE
• Molecular weight: Theoretical: 748.065 Da

Chemical component information

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipid*YM

Macromolecule #49: CARDIOLIPIN

• Macromolecule: Name: CARDIOLIPIN / type: ligand / ID: 49 / Number of copies: 6 / Formula: CDL
• Molecular weight: Theoretical: 1.464043 KDa

Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

Macromolecule #50: DODECYL-BETA-D-MALTOSIDE

• Macromolecule: Name: DODECYL-BETA-D-MALTOSIDE / type: ligand / ID: 50 / Number of copies: 2 / Formula: LMT
• Molecular weight: Theoretical: 510.615 Da

Chemical component information

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

Macromolecule #51: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 51 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #52: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

• Macromolecule: Name: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE; type: ligand / ID: 52 / Number of copies: 1 / Formula: NDP
• Molecular weight: Theoretical: 745.421 Da

Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

Macromolecule #53: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 53 / Number of copies: 1 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #54: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butan...

• Macromolecule: Name: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate; type: ligand / ID: 54 / Number of copies: 2 / Formula: EHZ
• Molecular weight: Theoretical: 584.703 Da

Chemical component information

ChemComp-EHZ:
~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 3.82 mg/mL

Buffer

pH: 7.14
Component:

Concentration	Formula	Name
20.0 mM	NH2C(CH2OH)3	tris
150.0 mM	NaCl	sodium chloride
0.05 %	C24H46O11	DDM
2.5 mM	C16H19N2NaO14S2	BS3

Details: pH was corrected at room temperature ~22 C

• Grid: Model: UltrAuFoil / Material: GOLD / Mesh: 300 / Support film - Material: GOLD / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 90 sec. / Pretreatment - Atmosphere: AIR; Details: The grid was also covalently modified by 48 hour incubation in 5 mM HS-C11-EG6 in ethanol in an anoxic glovebox, and washed thrice in ethanol before air drying.
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 45.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.9 µm / Nominal defocus min: 1.5 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: OTHER; Details: ACADVL is associated with complex I and the startup model used was EMD-11377.
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 10005
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD