[English] 日本語
![](img/lk-miru.gif)
- EMDB-16032: Structure of mouse heavy chain apoferritin solved by subtomogram ... -
+
Open data
-
Basic information
Entry | ![]() | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Structure of mouse heavy chain apoferritin solved by subtomogram averaging of 100 tilt series in Warp/M | |||||||||
![]() | ||||||||||
![]() |
| |||||||||
![]() | Apoferritin / Subtomogram averaging / STA / METAL BINDING PROTEIN | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | subtomogram averaging / cryo EM / Resolution: 1.6 Å | |||||||||
![]() | Obr M / Yang W / Karia D / Koh FA / Kotecha A | |||||||||
Funding support | 1 items
| |||||||||
![]() | ![]() Title: Structure of mouse heavy chain apoferritin solved by subtomogram averaging of 10 tilt series in Warp/M Authors: Obr M / Yang W / Karia D / Koh FA / Kotecha A | |||||||||
History |
|
-
Structure visualization
Supplemental images |
---|
-
Downloads & links
-EMDB archive
Map data | ![]() | 23 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 15.1 KB 15.1 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 13.5 KB | Display | ![]() |
Images | ![]() | 154.2 KB | ||
Filedesc metadata | ![]() | 4.4 KB | ||
Others | ![]() ![]() | 110.9 MB 110.9 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
EMDB pages | ![]() ![]() |
---|
-
Map
File | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Voxel size | X=Y=Z: 0.729 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Half map: #1
File | emd_16032_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #2
File | emd_16032_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-
Sample components
-Entire : Ferritin heavy chain
Entire | Name: Ferritin heavy chain |
---|---|
Components |
|
-Supramolecule #1: Ferritin heavy chain
Supramolecule | Name: Ferritin heavy chain / type: complex / ID: 1 / Parent: 0 |
---|---|
Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | subtomogram averaging |
Aggregation state | particle |
-
Sample preparation
Concentration | 5 mg/mL | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Buffer | pH: 7.5 Component:
| |||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV |
-
Electron microscopy
Microscope | TFS KRIOS |
---|---|
Specialist optics | Energy filter - Name: TFS Selectris X / Energy filter - Slit width: 10 eV |
Details | Tilt series acquired with Hagen scheme +-48 degrees with 3 increment |
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number grids imaged: 1 / Average exposure time: 0.5 sec. / Average electron dose: 3.5 e/Å2 / Details: Imaged with TFS Selectris X and Falcon 4i |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 165000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |