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Yorodumi- EMDB-1592: Structure of the anaphase promoting complex interacting with a mi... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-1592 | |||||||||
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Title | Structure of the anaphase promoting complex interacting with a mitotic checkpoint complex | |||||||||
Map data | 3D map of the apo anaphase promoting complex | |||||||||
Sample |
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Keywords | Anaphase-promoting complex / Spindle checkpoint / Electron cryomicroscopy / Mitotic checkpoint complex / Cdc20 | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 20.0 Å | |||||||||
Authors | Herzog F / Primorac I / Dube P / Lenart P / Sander B / Mechtler K / Stark H / Peters JM | |||||||||
Citation | Journal: Science / Year: 2009 Title: Structure of the anaphase-promoting complex/cyclosome interacting with a mitotic checkpoint complex. Authors: Franz Herzog / Ivana Primorac / Prakash Dube / Peter Lenart / Björn Sander / Karl Mechtler / Holger Stark / Jan-Michael Peters / Abstract: Once all chromosomes are connected to the mitotic spindle (bioriented), anaphase is initiated by the protein ubiquitylation activity of the anaphase-promoting complex/cyclosome (APC/C) and its ...Once all chromosomes are connected to the mitotic spindle (bioriented), anaphase is initiated by the protein ubiquitylation activity of the anaphase-promoting complex/cyclosome (APC/C) and its coactivator Cdc20 (APC/C(Cdc20)). Before chromosome biorientation, anaphase is delayed by a mitotic checkpoint complex (MCC) that inhibits APC/C(Cdc20). We used single-particle electron microscopy to obtain three-dimensional models of human APC/C in various functional states: bound to MCC, to Cdc20, or to neither (apo-APC/C). These experiments revealed that MCC associates with the Cdc20 binding site on APC/C, locks the otherwise flexible APC/C in a "closed" state, and prevents binding and ubiquitylation of a wide range of different APC/C substrates. These observations clarify the structural basis for the inhibition of APC/C by spindle checkpoint proteins. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_1592.map.gz | 3.5 MB | EMDB map data format | |
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Header (meta data) | emd-1592-v30.xml emd-1592.xml | 6.8 KB 6.8 KB | Display Display | EMDB header |
Images | EMDB_1592.gif | 57.4 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-1592 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-1592 | HTTPS FTP |
-Validation report
Summary document | emd_1592_validation.pdf.gz | 226.8 KB | Display | EMDB validaton report |
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Full document | emd_1592_full_validation.pdf.gz | 225.9 KB | Display | |
Data in XML | emd_1592_validation.xml.gz | 5 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-1592 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-1592 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_1592.map.gz / Format: CCP4 / Size: 3.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | 3D map of the apo anaphase promoting complex | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 3.8 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : Anaphase promoting complex
Entire | Name: Anaphase promoting complex |
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Components |
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-Supramolecule #1000: Anaphase promoting complex
Supramolecule | Name: Anaphase promoting complex / type: sample / ID: 1000 / Number unique components: 15 |
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Molecular weight | Theoretical: 1.7 MDa |
-Macromolecule #1: apo Anaphase Promoting Complex
Macromolecule | Name: apo Anaphase Promoting Complex / type: protein_or_peptide / ID: 1 / Name.synonym: APC / Recombinant expression: No |
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Source (natural) | Organism: Homo sapiens (human) / synonym: Human / Cell: HeLa |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Vitrification | Cryogen name: NITROGEN / Instrument: OTHER |
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-Electron microscopy
Microscope | FEI/PHILIPS CM200FEG/ST |
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Image recording | Category: CCD / Film or detector model: GENERIC TVIPS (4k x 4k) |
Electron beam | Acceleration voltage: 160 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD |
Sample stage | Specimen holder: Eucentric / Specimen holder model: GATAN LIQUID NITROGEN |
-Image processing
Details | GraFix treated |
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Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 20.0 Å |