ジャーナル: Nat Commun / 年: 2022 タイトル: Structures of the eukaryotic ribosome and its translational states in situ. 著者: Patrick C Hoffmann / Jan Philipp Kreysing / Iskander Khusainov / Maarten W Tuijtel / Sonja Welsch / Martin Beck / 要旨: Ribosomes translate genetic information into primary structure. During translation, various cofactors transiently bind to the ribosome that undergoes prominent conformational and structural changes. ...Ribosomes translate genetic information into primary structure. During translation, various cofactors transiently bind to the ribosome that undergoes prominent conformational and structural changes. Different translational states of ribosomes have been well characterized in vitro. However, to which extent the known translational states are representative of the native situation inside cells has thus far only been addressed in prokaryotes. Here, we apply cryo-electron tomography to cryo-FIB milled Dictyostelium discoideum cells combined with subtomogram averaging and classification. We obtain an in situ structure that is locally resolved up to 3 Angstrom, the distribution of eukaryotic ribosome translational states, and unique arrangement of rRNA expansion segments. Our work demonstrates the use of in situ structural biology techniques for identifying distinct ribosome states within the cellular environment.
全体 : 80S ribosome from inside Dictyostelium discoideum cells.
全体
名称: 80S ribosome from inside Dictyostelium discoideum cells.
要素
複合体: 80S ribosome from inside Dictyostelium discoideum cells.
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超分子 #1: 80S ribosome from inside Dictyostelium discoideum cells.
超分子
名称: 80S ribosome from inside Dictyostelium discoideum cells. タイプ: complex / ID: 1 / 親要素: 0 詳細: Cells from axenic Dictyostelium discoideum strain Ax2-214 with integrated GFP-Nup62 grown in HL5 medium were plunge frozen on Au 200mesh EM support grids.
由来(天然)
生物種: Dictyostelium discoideum AX2 (キイロタマホコリカビ) 株: Ax2-214 / 細胞中の位置: cytoplasm of perinuclear region