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- EMDB-15801: cryo-EM structure of carboxysomal mini-shell: icosahedral assembl... -
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Open data
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Basic information
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Title | cryo-EM structure of carboxysomal mini-shell: icosahedral assembly from CsoS4A/1A and CsoS2 co-expression (T = 9) | |||||||||
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![]() | carboxysome / shell / icosahedral symmetry / STRUCTURAL PROTEIN | |||||||||
Function / homology | ![]() structural constituent of carboxysome shell / carboxysome / carbon fixation Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 1.86 Å | |||||||||
![]() | Ni T / Jiang Q / Liu LN / Zhang P | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Intrinsically disordered CsoS2 acts as a general molecular thread for α-carboxysome shell assembly. Authors: Tao Ni / Qiuyao Jiang / Pei Cing Ng / Juan Shen / Hao Dou / Yanan Zhu / Julika Radecke / Gregory F Dykes / Fang Huang / Lu-Ning Liu / Peijun Zhang / ![]() ![]() Abstract: Carboxysomes are a paradigm of self-assembling proteinaceous organelles found in nature, offering compartmentalisation of enzymes and pathways to enhance carbon fixation. In α-carboxysomes, the ...Carboxysomes are a paradigm of self-assembling proteinaceous organelles found in nature, offering compartmentalisation of enzymes and pathways to enhance carbon fixation. In α-carboxysomes, the disordered linker protein CsoS2 plays an essential role in carboxysome assembly and Rubisco encapsulation. Its mechanism of action, however, is not fully understood. Here we synthetically engineer α-carboxysome shells using minimal shell components and determine cryoEM structures of these to decipher the principle of shell assembly and encapsulation. The structures reveal that the intrinsically disordered CsoS2 C-terminus is well-structured and acts as a universal "molecular thread" stitching through multiple shell protein interfaces. We further uncover in CsoS2 a highly conserved repetitive key interaction motif, [IV]TG, which is critical to the shell assembly and architecture. Our study provides a general mechanism for the CsoS2-governed carboxysome shell assembly and cargo encapsulation and further advances synthetic engineering of carboxysomes for diverse biotechnological applications. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 1.3 GB | ![]() | |
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Header (meta data) | ![]() ![]() | 18.9 KB 18.9 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 25.1 KB | Display | ![]() |
Images | ![]() | 227.8 KB | ||
Masks | ![]() | 1.4 GB | ![]() | |
Others | ![]() ![]() | 1.1 GB 1.1 GB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8b12MC ![]() 8b0yC ![]() 8b11C C: citing same article ( M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | main map | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.831 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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-Half map: half map 2
File | emd_15801_half_map_1.map | ||||||||||||
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Annotation | half map 2 | ||||||||||||
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-Half map: half map 1
File | emd_15801_half_map_2.map | ||||||||||||
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Annotation | half map 1 | ||||||||||||
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Sample components
-Entire : mini-shell assembly from Halothiobacillus neapolitanus with CsoS4...
Entire | Name: mini-shell assembly from Halothiobacillus neapolitanus with CsoS4A/1A and CsoS2 co-expression (T= 9) |
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Components |
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-Supramolecule #1: mini-shell assembly from Halothiobacillus neapolitanus with CsoS4...
Supramolecule | Name: mini-shell assembly from Halothiobacillus neapolitanus with CsoS4A/1A and CsoS2 co-expression (T= 9) type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3 |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Major carboxysome shell protein CsoS1A
Macromolecule | Name: Major carboxysome shell protein CsoS1A / type: protein_or_peptide / ID: 1 / Number of copies: 8 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 9.973478 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MADVTGIALG MIETRGLVPA IEAADAMTKA AEVRLVGRQF VGGGYVTVLV RGETGAVNAA VRAGADACER VGDGLVAAHI IARVHSEVE NILPKAPQA UniProtKB: Major carboxysome shell protein CsoS1A |
-Macromolecule #2: Carboxysome shell vertex protein CsoS4A
Macromolecule | Name: Carboxysome shell vertex protein CsoS4A / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 8.900287 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MKIMQVEKTL VSTNRIADMG HKPLLVVWEK PGAPRQVAVD AIGCIPGDWV LCVGSSAARE AAGSKSYPSD LTIIGIIDQW NGE UniProtKB: Carboxysome shell vertex protein CsoS4A |
-Macromolecule #3: Carboxysome assembly protein CsoS2B
Macromolecule | Name: Carboxysome assembly protein CsoS2B / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 91.502031 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MNPADLSGLS GKELARARRA ALSKQGKAAV SNKTASVNRS TKQAASSINT NQVRSSVNEV PTDYQMADQL CSTIDHADFG TESNRVRDL CRQRREALST IGKKAVKTNG KPSGRVRPQQ SVVHNDAMIE NAGDTNQSSS TSLNNELSEI CSIADDMPER F GSQAKTVR ...String: MNPADLSGLS GKELARARRA ALSKQGKAAV SNKTASVNRS TKQAASSINT NQVRSSVNEV PTDYQMADQL CSTIDHADFG TESNRVRDL CRQRREALST IGKKAVKTNG KPSGRVRPQQ SVVHNDAMIE NAGDTNQSSS TSLNNELSEI CSIADDMPER F GSQAKTVR DICRARRQAL SERGTRAVPP KPQSQGGPGR NGYQIDGYLD TALHGRDAAK RHREMLCQYG RGTAPSCKPT GR VKNSVQS GNAAPKKVET GHTLSGGSVT GTQVDRKSHV TGNEPGTCRA VTGTEYVGTE QFTSFCNTSP KPNATKVNVT TTA RGRPVS GTEVSRTEKV TGNESGVCRN VTGTEYMSNE AHFSLCGTAA KPSQADKVMF GATARTHQVV SGSDEFRPSS VTGN ESGAK RTITGSQYAD EGLARLTING APAKVARTHT FAGSDVTGTE IGRSTRVTGD ESGSCRSISG TEYLSNEQFQ SFCDT KPQR SPFKVGQDRT NKGQSVTGNL VDRSELVTGN EPGSCSRVTG SQYGQSKICG GGVGKVRSMR TLRGTSVSGQ QLDHAP KMS GDERGGCMPV TGNEYYGREH FEPFCTSTPE PEAQSTEQSL TCEGQIISGT SVDASDLVTG NEIGEQQLIS GDAYVGA QQ TGCLPTSPRF NQTGNVQSMG FKNTNQPEQN FAPGEVMPTD FSIQTPARSA QNRITGNDIA PSGRITGPGM LATGLITG T PEFRHAAREL VGSPQPMAMA MANRNKAAQA PVVQPEVVAT QEKPELVCAP RSDQMDRVSG EGKERCHITG DDWSVNKHI TGTAGQWASG RNPSMRGNAR VVETSAFANR NVPKPEKPGS KITGSSGNDT QGSLITYSGG ARG UniProtKB: Carboxysome assembly protein CsoS2B |
-Macromolecule #4: water
Macromolecule | Name: water / type: ligand / ID: 4 / Number of copies: 485 / Formula: HOH |
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Molecular weight | Theoretical: 18.015 Da |
Chemical component information | ![]() ChemComp-HOH: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 44.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |