EMDB-15100: Structure of the mitochondrial ribosome from Polytomella magna

Basic information

Entry

Database: EMDB / ID: EMD-15100

• Title: Structure of the mitochondrial ribosome from Polytomella magna
• Map data: Consensus refinement using solvent mask

Sample

• Complex: Mitochondrial ribosome from Polytomella magna
  • RNA: x 13 types
  • Protein or peptide: x 104 types
• Ligand: x 5 types

• Biological species: Polytomella magna (plant)
• Method: single particle reconstruction / cryo EM / Resolution: 2.91 Å
• Authors: Tobiasson V / Berzina I / Amunts A

Funding support

Sweden, 1 items

Organization	Grant number	Country
Swedish Research Council		Sweden

• Citation: Journal: Nat Commun / Year: 2022; Title: Structure of a mitochondrial ribosome with fragmented rRNA in complex with membrane-targeting elements.; Authors: Victor Tobiasson / Ieva Berzina / Alexey Amunts / ; Abstract: Mitoribosomes of green algae display a great structural divergence from their tracheophyte relatives, with fragmentation of both rRNA and proteins as a defining feature. Here, we report a 2.9 Å resolution structure of the mitoribosome from the alga Polytomella magna harbouring a reduced rRNA split into 13 fragments. We found that the rRNA contains a non-canonical reduced form of the 5S, as well as a permutation of the LSU domain I. The mt-5S rRNA is stabilised by mL40 that is also found in mitoribosomes lacking the 5S, which suggests an evolutionary pathway. Through comparison to other ribosomes with fragmented rRNAs, we observe that the pattern is shared across large evolutionary distances, and between cellular compartments, indicating an evolutionary convergence and supporting the concept of a primordial fragmented ribosome. On the protein level, eleven peripherally associated HEAT-repeat proteins are involved in the binding of 3' rRNA termini, and the structure features a prominent pseudo-trimer of one of them (mL116). Finally, in the exit tunnel, mL128 constricts the tunnel width of the vestibular area, and mL105, a homolog of a membrane targeting component mediates contacts with an inner membrane bound insertase. Together, the structural analysis provides insight into the evolution of the ribosomal machinery in mitochondria.

History

Deposition	Jun 2, 2022	-
Header (metadata) release	Nov 16, 2022	-
Map release	Nov 16, 2022	-
Update	Nov 23, 2022	-
Current status	Nov 23, 2022	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_15100.map.gz / Format: CCP4 / Size: 1.3 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Consensus refinement using solvent mask
• Voxel size: X=Y=Z: 0.846 Å

Density

Contour Level	By AUTHOR: 0.01
Minimum - Maximum	-0.018139599 - 0.05212105
Average (Standard dev.)	-0.000149564 (±0.0016416265)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 700 700 700 Spacing 700 700 700
Cell	A=B=C: 592.2 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_15100_msk_1.map

Additional map: Composite map, constructed from 6 masked and local...

• File: emd_15100_additional_1.map
• Annotation: Composite map, constructed from 6 masked and local resolution filtered maps

Half map: Half map #1 for consensus refinement

• File: emd_15100_half_map_1.map
• Annotation: Half map #1 for consensus refinement

Half map: Half map #2 for consensus refinement

• File: emd_15100_half_map_2.map
• Annotation: Half map #2 for consensus refinement

Sample components

Entire : Mitochondrial ribosome from Polytomella magna

• Entire: Name: Mitochondrial ribosome from Polytomella magna

Components

• Complex: Mitochondrial ribosome from Polytomella magna
  • RNA: mtLSU-1
  • RNA: mtLSU-2
  • RNA: mtLSU-3
  • RNA: mtLSU-4
  • RNA: mtLSU-5
  • RNA: mtLSU-6
  • RNA: mtLSU-7
  • RNA: mtLSU-8
  • RNA: mt-5S
  • Protein or peptide: uL2m
  • Protein or peptide: uL3m
  • Protein or peptide: uL4m
  • Protein or peptide: uL5m
  • Protein or peptide: uL6m
  • Protein or peptide: uL9m
  • Protein or peptide: uL13m
  • Protein or peptide: uL14m
  • Protein or peptide: uL15m
  • Protein or peptide: uL16m
  • Protein or peptide: bL17m
  • Protein or peptide: uL18m
  • Protein or peptide: bL19m
  • Protein or peptide: bL20m
  • Protein or peptide: bL21m
  • Protein or peptide: uL22m
  • Protein or peptide: uL23m
  • Protein or peptide: bL24m
  • Protein or peptide: bL25m
  • Protein or peptide: bL27m
  • Protein or peptide: bL28m
  • Protein or peptide: uL29m
  • Protein or peptide: uL30m
  • Protein or peptide: bL31m
  • Protein or peptide: bL31m
  • Protein or peptide: bL33m
  • Protein or peptide: bL34m
  • Protein or peptide: bL35m
  • Protein or peptide: bL36m
  • Protein or peptide: mL40
  • Protein or peptide: mL41
  • Protein or peptide: mL43
  • Protein or peptide: mL46
  • Protein or peptide: mL63
  • Protein or peptide: mL64Microsoft Macro Assembler
  • Protein or peptide: mL87
  • Protein or peptide: mL116
  • Protein or peptide: mL118
  • Protein or peptide: mL120
  • Protein or peptide: mL121
  • Protein or peptide: mL122
  • Protein or peptide: mL123
  • Protein or peptide: mL124
  • Protein or peptide: mL125
  • Protein or peptide: mL126
  • Protein or peptide: mL127
  • Protein or peptide: mL128
  • Protein or peptide: mL129
  • RNA: mtSSU-1
  • RNA: mtSSU-2
  • RNA: mtSSU-3
  • RNA: mtSSU-4
  • Protein or peptide: bS1m
  • Protein or peptide: uS2m
  • Protein or peptide: uS3m
  • Protein or peptide: uS4m
  • Protein or peptide: uS5m
  • Protein or peptide: uS6m
  • Protein or peptide: uS7m
  • Protein or peptide: uS8m
  • Protein or peptide: uS9m
  • Protein or peptide: uS10m
  • Protein or peptide: uS11m
  • Protein or peptide: uS12m
  • Protein or peptide: uS13m
  • Protein or peptide: uS14m
  • Protein or peptide: uS15m
  • Protein or peptide: bS16m
  • Protein or peptide: uS17m
  • Protein or peptide: bS18m
  • Protein or peptide: bS19m
  • Protein or peptide: bS21m
  • Protein or peptide: mS23
  • Protein or peptide: mS26
  • Protein or peptide: mS29
  • Protein or peptide: mS31
  • Protein or peptide: mS33
  • Protein or peptide: mS34
  • Protein or peptide: mS35
  • Protein or peptide: mS37
  • Protein or peptide: mS38
  • Protein or peptide: mS45
  • Protein or peptide: mS106
  • Protein or peptide: mS107
  • Protein or peptide: uS4m-2 (fragment)
  • Protein or peptide: mS108
  • Protein or peptide: mS109
  • Protein or peptide: mS110
  • Protein or peptide: uS3m-2 (fragment)
  • Protein or peptide: mS111
  • Protein or peptide: mS112
  • Protein or peptide: mS113
  • Protein or peptide: mS114
  • Protein or peptide: mS115
  • Protein or peptide: mS116
  • Protein or peptide: uS7m-2 (fragment)
  • Protein or peptide: Unknown
  • Protein or peptide: mL105
  • Protein or peptide: Unknown
  • Protein or peptide: Unknown
  • Protein or peptide: Unknown
  • Protein or peptide: Unknown
  • Protein or peptide: Unknown
  • Protein or peptide: Unknown
  • Protein or peptide: Unknown
  • Protein or peptide: Unknown
  • Protein or peptide: Unknown
  • Protein or peptide: Unknown
• Ligand: MAGNESIUM ION
• Ligand: POTASSIUM IONPotassium
• Ligand: ZINC ION
• Ligand: ADENOSINE-5'-TRIPHOSPHATE
• Ligand: water

Supramolecule #1: Mitochondrial ribosome from Polytomella magna

• Supramolecule: Name: Mitochondrial ribosome from Polytomella magna / type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: #1-#117
• Source (natural): Organism: Polytomella magna (plant)

Macromolecule #1: mtLSU-1

• Macromolecule: Name: mtLSU-1 / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 35.302168 KDa

Sequence

String:

UGUGACCGUU AGCGCAUAGU ACAGUAAUGG AAGUGCAUAA ACCAAAUUAA AGGCUUAAAU UAAAAAUAAA AUGGCUGGUG CAAUGGCAC AAAAAAUAAG AAAGUUUAAA

Macromolecule #2: mtLSU-2

• Macromolecule: Name: mtLSU-2 / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 26.056578 KDa

Sequence

String:

AAUAAAUAAG UUUAAUACCU UUUGCAGCAU GGACCAGCGA UUAAAUGCCG GUAAUUAGCA AAGUUAAACU AACGAAAAAU A

Macromolecule #3: mtLSU-3

• Macromolecule: Name: mtLSU-3 / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 67.578062 KDa

Sequence

String:

UUACAGGCAU UUAAUUCGAA GCAUAGCGAG CUAACGUUUA GCAGGUUUGC AUAAAUUAAA UAUAUUAAAA AAGCAAGACC GCAAAGGUU UCUGUAGCAA UAGAAUCUUG UGACUUAACG UUAGGGGCGA UAAACCAAUC ACGCUAUGGC AUAGCUAAGU U UCUGCGAA AUAUAUUUAG GUAUAGCUUA UGCAAAAACU UUAUAGAUAA AA

Macromolecule #4: mtLSU-4

• Macromolecule: Name: mtLSU-4 / type: rna / ID: 4 / Number of copies: 1
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 23.661262 KDa

Sequence

String:

UAAGUCAGAC GCAAAGCUAC AAGGUUUCGC GUCAAAAGGA AAAAAGUCCG CCAGCGAGGC UAAGGUACAU AAA

Macromolecule #5: mtLSU-5

• Macromolecule: Name: mtLSU-5 / type: rna / ID: 5 / Number of copies: 1
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 45.703215 KDa

Sequence

String:

UAAUGUGCAC GCAAACAACC AGGAGGUGGG CUUGGAAGCA GCCAUCCUUU GAAGAAAGCG UAAUAGCUCU GCUUUUUAGU GUAACAUUA CACUACACGC AGAAAAUAAA AGCCGAAGCU CGCUUGUUAA UACAGUUAAA UAU

Macromolecule #6: mtLSU-6

• Macromolecule: Name: mtLSU-6 / type: rna / ID: 6 / Number of copies: 1
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 42.369309 KDa

Sequence

String:

GCAGGUAGCA GAACGUUCUA UAUAACUUUA AAGUAAUAGA AUUGCUAAUG CUGGUAUAAG UAACUAUGUA AAAAUACAGC UAAGCUAAA GGUUUUGCGC AACAGUGAAA CGAUAUAGAU AAAGGAAAGU AA

Macromolecule #7: mtLSU-7

• Macromolecule: Name: mtLSU-7 / type: rna / ID: 7 / Number of copies: 1
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 185.617266 KDa

Sequence

String:

AAUCGUACCG CAAACCAACA CAGGUUAGUU AUUGUAAAGG CUGUAUAAAA CCAGGAUAAA GGAACUCGGC AAAAUAGGUU AUGUUUUCG UACAAAACCA CAACAAAAGU AGUGACUGUU UACCAAAAAC ACAAGACUCU GCUAACACUU AUAGUGGAAG U AUAGUGUC UGAUACCUGC CCAAAGGCAA AGCAAACGGC AGCUGUAACU CUAACGGCUC UAAGGUAGCA AAAUUCCAUG GC AUUUAAA UGAUGUCCCG CAUGAAUGGU AUAACAACUU CUUAACUGUC UCUAUUCUGG AUUAGGCGAA CUUUAAAUGC UUG UGCAGA UGCAGGCUAG CUAACAAGGG ACGAAGAGAC CCUAUGCAGC UUUAGGGCAG GUUAUUAUUU AAAGAGUAAA AAAU AGAAA AACUAUAAAA CUAUAAAAAU AACAAUAAAC UACCUUUUUU CUGGGACGGA CGCUCUUAUU AAAGAAACAA GAGUG AGCA AUGGUUUUUA CUAAUUAUAA AAAAGAAAAG CUUAACUGCA AUACAAACAA UUAAAGCAGG GGCAUUGUCG GCAAUA AUG CAUUGAAGAA AUA

Macromolecule #8: mtLSU-8

• Macromolecule: Name: mtLSU-8 / type: rna / ID: 8 / Number of copies: 1
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 112.621266 KDa

Sequence

String:

CGUUCUUCAA CCAACGGAAU AAAGCUACGC UAGGGAUAAC AGGCUUAUUC GCUCACAGAG UUUUAUCGAC GAGCGCGGUU GGCACCUCG AUGUUGGAUC ACCGCUUCCU GUAGCUGCAA ACGGUUAUAA GGGUUAGACU GUUCGUCUGA UAAUGCGGUA C GUGAUCUG AGUUCAAAGC GUCGUAAGAC AGUUUGGUUU CUAUCUUUGU UAGCAUAAGU AUUACGGCGU UGUGUACAGU AC GAAAGGA UUGUUGCACA AUUAACCAAU AGUUUAAAGA GCAACGUUAA UAAACCGGAA AACUUUUAAC UGCACUGUGC AGU UGCAGG CAAAGCAAUG UUCAGCUAAA AAAA

Macromolecule #9: mt-5S

• Macromolecule: Name: mt-5S / type: rna / ID: 9 / Number of copies: 1
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 22.130088 KDa

Sequence

String:

UAAUGCGAUA AUACGCAUGA CGGUUCAUUU UUGCCGUGUG UGGUCACGGU AAAACUUAAG AAUCGUCCA

Macromolecule #58: mtSSU-1

• Macromolecule: Name: mtSSU-1 / type: rna / ID: 58 / Number of copies: 1
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 32.708301 KDa

Sequence

String:

CAUAGGGUUU GGUUUUUGCC CAGCCUUUAG CUUAGCGUUA AGCUUAUACA UGCUAGCCUA AAAGACGGCG CACAGGUGCA UAUUUUGUA AGCAUGUAAA AUA

Macromolecule #59: mtSSU-2

• Macromolecule: Name: mtSSU-2 / type: rna / ID: 59 / Number of copies: 1
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 68.920906 KDa

Sequence

String:

AAAGUGUACU UUAUAAAGUC AUUGUAUUGC UUACAAAUAA CUAGGCAGUU GGGUCGGCUG CUAUACUAUA GCGCCCCAAG CCUGAUAAU UAGCUAGGCG UAACAGCGGC AAGCCACAGU AGCACAUAAA AAUUGCUACC GUUUAUUUAC GCAGCAGUAA G AAAGAUUG GACAAUAGCU UAAGGGCAGA UCCAGGUAAG CGCUAAAAUC UUUGUG

Macromolecule #60: mtSSU-3

• Macromolecule: Name: mtSSU-3 / type: rna / ID: 60 / Number of copies: 1
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 122.239391 KDa

Sequence

String:

GAGCUCCGGC UAAUUGUGUG CCAGCAGCCG CGGGAAUACA CAAGGGGCCA GUUUAAGGUU AAAACAAGGC AACGGUGUUU AAAAACUGG AAAUGCGCCU AAAAUAGCUU AAUGCGUUGG AGUAAGGGUU AAAUCUGUAA AAACCUUGCA GAAAGCCUUA A AGUAAAAG CAGCUAUUCA UGCCCAUAAA ACAGUCUAUA AACGAGAGCU GCGGUAGCAA AACGGAUUAG AUACCCGUGU AG UCCCAGC CGUAAAUUAU UUAUUAAAUU AAAGCGCCUA GCGAUAUUGC CUAUGGCACU AAACUAGGGA AUCUGACAGC AUU AAACUG ACGCGGUGGA GCAUACUUCU UAAUUUGAUA AUACGCAAAA ACAUUACCAC GAU

Macromolecule #61: mtSSU-4

• Macromolecule: Name: mtSSU-4 / type: rna / ID: 61 / Number of copies: 1
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 112.061297 KDa

Sequence

String:

AAACCACGGU AUUGCAUGGC UGAUAUAUAA UCCAUACAAA GGAUAUAACC AAGUAGCAUG GUCUUAUAUU GUGGGCUGGC GGUGUGCUA CAAGUAACAA AAAGUACAAC CGGAUUAAUU ACUGAAAUCA UAAAUAUAAA GAGGGAAUCG CUAGUAAUCU U UAUUGUAA UAACAUAAAG GUGAACUAAC AAUACAGUUU AGUGUGCACA CACUGCCCGU CACAUCCGGG AAAACCACAA AG CCCUAAG UUUGUAUAGU GUUUAAUAAA CCAAUUCAAA CAUAAGCUUA UUGUUAACAA GGAUGAAGUC GUAACAAGGU UUG CGUAGG GGAACCUGCU CAAGAUUAUG CU

Macromolecule #10: uL2m

• Macromolecule: Name: uL2m / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 33.873227 KDa

Sequence

String:

KYTYKFKQAR AVPGLVYWKP RTPGLRHKIS IDYDFLGVYT GAPHKDLSAA VPKLSGRNNH GRITARHRGG GMKKVLRQVD YSREKVDGI PGVVERVEKD PNRSGFLALV KYQPEGQLPI FKYHLAPAEI KAGDVLLSGD GAPVQAGSTL PLRSIPLGTP I HNLELTPG KGGQLARAAN VSAVIQSKQL ENAIVKLPSG EIRLINLDCR ATVGRVSNHL QRMINYGKAG ERRKLGWRPS VR GIAKNPN DHPHGGRTNG GRPSCTPWGV YCKGQRTRRR NNYTNKFIIT RKGGQPIRKF AEAKKLKAR

Macromolecule #11: uL3m

• Macromolecule: Name: uL3m / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 34.287707 KDa

Sequence

String:

GYRPAKPVYP LPDSIASLVE DKIAELKKFG PTPEPALEPL PLTPDSVRVG CITVKAGMTH EWDEHGVMIP LTVLWIDECQ VVGLKYFEK NGYYAVQLGA GCVKQHTVLP QIAGYYLKHD LPIKREMKEY QVSSPEALLP VGTHITAAHF VVGQQVDVTG Y TTDKGFQG VMKRWGFKGM PASHGVSLSH RKPGATGGRQ DPGKIWKEKK LPGHMGDEKR TQLGLELYKV DPKRNLLYVK GA VPGPRGA SILVRDSNRM DYEERVALNL PYPTFIGSPD ELPVLVRKSD KDPYLMYKKE IDYFPIKWK

Macromolecule #12: uL4m

• Macromolecule: Name: uL4m / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 33.741516 KDa

Sequence

String:

VASYLARICP NTYVPPPFVA TKKGFNGIGG RYDPSSPFPP DTGSSPLTLQ YPFEVEYHKD REIPVCNVSD GSQVSTTTLN GKIFSDKVR LDILHTVVRY LRAKWQQGTH KTKDRSEVSG GGRKPRPQKG SGRSRQGSIR SPIWRGGGCT FPKIPRSHAF K LPRNVVRI GIRSALSAKA NEGRLFVVDS FVRGVESYDQ LKAGLAEVTK DAIGESLLLV DSGECGEDYS GVKLRRLLPK DS PRVEVLS YQDLTVYHML KYHKLVVSEP AVRLIEQELT RPLRNPARAA FWQEREARIG AAVEDL

Macromolecule #13: uL5m

• Macromolecule: Name: uL5m / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 21.894408 KDa

Sequence

String:

SSLHLTSRYH HMFYNVLYRE MMLKAPKTKV EQGPHLESIT LTIKTTDTRL DKDIVDKWEL LLFSLALEHI TGEPAAFLPP PNRHVANKS AGVTVTLKGE KMFNFLEKLV YTILPNQTGF EGVPQPKTLV PGPDGVVKRV VDFGVTNLLL YPDFEEHFGV F EPIRSMQV RMMIRSDSDE SCNMLVSGLL VPQLE

Macromolecule #14: uL6m

• Macromolecule: Name: uL6m / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 27.446111 KDa

Sequence

String:

YKIPDKIKWP KTPYSKICGR PAPEAYPIFE ILKNADKPDV WQRSLIRFPE GVEARVDEET RVLHLEGKAG SVSINMKNLD PTGLLAYSF EQNSLVMLSP SKEKLNAFVQ EIQKSVRGAS QGYLVGLTVK GVGYRMEPVE DIPAKYLSWS RPVYFDRTTP P AYPFKKPA KAVLLKIGFT DPAVFMLPPT IKAFFLKPTL MYLYGLNIDE LRKTAMDIRA LRKPNVFTGN GIALLDETVR IK QR

Macromolecule #15: uL9m

• Macromolecule: Name: uL9m / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 6.16306 KDa

Sequence

String:

VEESNGKLVK IVLLDDVDRV GQQGQTVVVK AGLMRHLLFP EGKALYATPE NEAKYS

Macromolecule #16: uL13m

• Macromolecule: Name: uL13m / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 21.549129 KDa

Sequence

String:

MQPILNHTFP RNVQLRALKE LKNIDLNGLR MRFVDAKGVG LGSLAMKVAT ILQGKDKPTY SPYRENGDVV VVVNAAHIYL SQDKWETMK YWWHTGYAGG ARELTAKQMW EKDPCQIIVR AVNGMLPKNV TRIQRMEKLK VFAEDEHPFK DFPLTPLVIE Q RKGFGWAP PEGFKPLNPE RFDLRRRI

Macromolecule #17: uL14m

• Macromolecule: Name: uL14m / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 13.522091 KDa

Sequence

String:

MLPNYSFLKV VDNSGAKLVK IIGFYGHPGK AVGVGEVVKV AIKEAKGEKV SAGEMKKAVI VEQKFPVFRP NGSHFRHLRN SCVLLSEKG QPLGNKVRAL LTYEFIKPRW SKLRTITTKK LF

Macromolecule #18: uL15m

• Macromolecule: Name: uL15m / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 22.826402 KDa

Sequence

String:

NPNYFVHLGN LRPNPGATQQ RIREGRGLSS RRGVSCGMGF KGQKARGRSL HMLYDGGQLG LVKFPVTRQI PSYEVLYNQL GVSKLVEFI QLGLLDHTKT ITMKELLNAG CVSSLKYGVL LYGNVSLSFP INIEVTACDD RARRSIEAAG GKVTRVYYTK D GLEGLLFP KRFTDKNLPL PRPAVAWHPK FDGKFDAVGQ IPPVYEAV

Macromolecule #19: uL16m

• Macromolecule: Name: uL16m / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 19.33423 KDa

Sequence

String:

FRRRTWPAVC GSLHIWKKMP WRIDQAPPPM PDSKPLTRLR AKRYEGIKKV VMPNSTQLQF GLYGIRSMES ARLKDNTLEA IRRTIRRKI KKTGRLWIMI RATVPMTKKP LGIRMGKGKG SIDHYVTPIR PGQILYEFDR ISRPIALQAL RAIQPKLPCR L GLVEWS

Macromolecule #20: bL17m

• Macromolecule: Name: bL17m / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 20.047506 KDa

Sequence

String:

TVKRFLRPGG WNRLNRPVKE RLALIKNMAT QLVEKERIRT TLSKAKNLQR YGDKLITLGK AGTRESWLKA NDIVRTDREL HKLFTTLAL RYKDRQGGYV RLIRAGFRRG DAAPVAIVEL VDREGELRQA RPAVTPVPKF SVMRWVKQQL DPKFISEPAP L LPPLVRDY LKEQN

Macromolecule #21: uL18m

• Macromolecule: Name: uL18m / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 12.928119 KDa

Sequence

String:

PLKPLVFRPL KLFFWATNRH VHAKVVRFSS PFNEGVPVVD ISTFDAFNKL KGSAPVVAPR SLECYKEVAK MVKEETARQN INEVTLHLN SHASDRGVRE VVRELKNLGL LVKKV

Macromolecule #22: bL19m

• Macromolecule: Name: bL19m / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 19.849031 KDa

Sequence

String:

EVIIPTIPKY ANPYKLTPGV SQRLRTPKAL YEPWCPSRLL LKKRNVRARM QFLLDVLETE KEVEFQKQRN YPDFKVGDVL DVTMVAAEN ERKTYVHRGL CIQKRDRGYG SSFKLYCVFS DSGGFIQHLP LCMPDILDIK IVGQVDYKDE EDLKELVESE S RECKFLDK VL

Macromolecule #23: bL20m

• Macromolecule: Name: bL20m / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 13.695711 KDa

Sequence

String:

TFSREQVFTF AKNFTGRARN VYKVALRRVE RGLQHSYVGR KEKKRTIRSL WIQRINAGCR EHGFAYSALI NGLNQENIQL NRKVLSELA MHEPDSFKAV LDQVRFMKGQ EIYANVEP

Macromolecule #24: bL21m

• Macromolecule: Name: bL21m / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 22.296104 KDa

Sequence

String:

VRTKLPSKRP FLQTASHLAV NSLVPSVRTN RWADLIKGPA VRSCIPEIPE VPKPSEYTTI GTIEGPYTLN LERVFGVVEL AGSQYKVTP DDVIFTNLLH GIEVNEVVML ERVMLLGSRS NTIIGRPYIP GACVLAAVEE HFLDGTVHVF KRKKRKRYRR Y KPTRPMMT TLRILKVLGI DPAPGDELGI PSGPVIKITN TE

Macromolecule #25: uL22m

• Macromolecule: Name: uL22m / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 21.768037 KDa

Sequence

String:

SRPPLYARSG KFRESEEVQP YRTWYWYDAE TESEERRKEL RQQLYRDSGM SYYLNIPQSM KKLERVVRVI RNLAYDEAWK QACVLPLKA AGFVKEGLEH ARKDAEEKGL DPKKLVVSTV YTAKGKYLRG FQPMGKGFAG RKTTRKTHLR IVLRENASVL P FAAQVVSP SRVGGASQKH LRDISVRYTV

Macromolecule #26: uL23m

• Macromolecule: Name: uL23m / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 18.235152 KDa

Sequence

String:

DYLLDDDING DPSNPLNKPM HFGSFQKQRQ PNSFRRLPIC FPDIKLTLMK LEEHQLEQIK QTGWLREAAF KTTPNTTKLE IKAFLESVY GMEVERVNTA NYLGRKRVVY TGKAKELYRE DDYKKAYVIF KKPEGLSLPE TRPLLQKLVD IKLKRK

Macromolecule #27: bL24m

• Macromolecule: Name: bL24m / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 13.088164 KDa

Sequence

String:

PLDIQVFRED TVRILNGPDK GLTGKVLEVF QDPKNPKIFV EGRNMRKKKI RMFVVISMEA PLDYKDVQLV DPATQEPGLL GPKDTSVEL AQKVTYSDPS HNRFPLSVRD LFAMFP

Macromolecule #28: bL25m

• Macromolecule: Name: bL25m / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 28.55024 KDa

Sequence

String:

QVVPSEIIRP LESINSFLEV KSNISGRIRY ELYSSELKFS FPKHDIEEGL LELTAYARTK TKKSRAKYLR EDGNIPTVIH GFDKATCGF HNLNLCIPEK EANAMVRKYG RNGCAARVVK VKVVDHANPE KVFGYLRTRP CSLAITATSL KLELMHLQYC P QDRLVDVR VPIRLINDDV APGVKKGGWI SVKQRLVGYQ CRGNSIPPHI EVDAKHLRLD DKVLKRDLPI PKGTRLLGCD FD AVVLHCT TDVGKE

Macromolecule #29: bL27m

• Macromolecule: Name: bL27m / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 16.266565 KDa

Sequence

String:

QGGKSHAKNW GPKFGDGEIV FPGMIIMRQK QGIYSPGYNV GRGRDYTIFA KTVGYVEYSK LKTERGKTRR YIHVVPINDD WSPEYQKLA DELVVRRAEV KRQILGHHVH TEPAFHFSLP QRNGNMSWLL NSVHKFPGTT TLA

Macromolecule #30: bL28m

• Macromolecule: Name: bL28m / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 15.075389 KDa

Sequence

String:

QQKNVEIARK LLPLAKKQLK GLLDRQDNVL WGKTDIVIRK RTFKKNDEEL YSLVQVQWLD VKLWSNILQE RVPLTLSQET LTHIESIGG LDDYLLKTPD SQMKSNFSSD LRWRVVCEAM KQGEAESKQK

Macromolecule #31: uL29m

• Macromolecule: Name: uL29m / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 14.509567 KDa

Sequence

String:

DLREFVDWSS REGASEAFGR AWNREELRLK SWDDLHKLWY VTIKERNLLL TELAWKEIPK TDEEQKARGI PRGSRQVETD VHKLRFEQA VKTLENIKVV LKERASSDPS KPKRKELLAI VNAK

Macromolecule #32: uL30m

• Macromolecule: Name: uL30m / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 20.863262 KDa

Sequence

String:

EPVRSLFVTL RRSYIGKAWF HREILQNFRL KKIRQTVEVA NTPSARNSLA KIPHLVTIET DKMYYLRNMK KFYEQLLRPP VQVSHEHAA LSSEIKPVHN LHSKHVQERL QEHAYPSKME GYVLHEKKPV VPRALRSFAF KSQDEHKSFV LARLGIFSEE A KKIHLNEL RTFAKRDR

Macromolecule #33: bL31m

• Macromolecule: Name: bL31m / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 8.388767 KDa

Sequence

String:

LVRPLTKAMT VVLSNGATLR LPTVYARAKP WFPVMDLHSH NVWKHKIKTD FQLESEKNIT PDFSNFYNKF GK

Macromolecule #34: bL31m

• Macromolecule: Name: bL31m / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 6.69001 KDa

Sequence

String:

AVPKKKLSVF RRRNRRIWYW KKASPIIGQC SHCGFVGPSG VLGLSPIAKC YPTEYKPPC

Macromolecule #35: bL33m

• Macromolecule: Name: bL33m / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 5.921157 KDa

Sequence

String:

RLLVKMVSLA KTGYFYVTTK NPRNTPWKLK LMKFDPVVGR HVLFEESKLK

Macromolecule #36: bL34m

• Macromolecule: Name: bL34m / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 6.149369 KDa

Sequence

String:

MKRGMTYQPS RKKRINKHGM EKRLGTEDGR LTILRRLEKG RWRLTVDMFR

Macromolecule #37: bL35m

• Macromolecule: Name: bL35m / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 16.67357 KDa

Sequence

String:

VFAEVKPRQN PQNHTHEKYK IIAPQPKYDW LVGRFIVDRN NVVWHRQANR NRNRHKKTAG ALTRLKRWKP LHKAYAKKLL KLGFKRRFW TDPDPQMVPG FFDPSKYKPR ERLNGKPNLR PDIGCPALRQ SQRPLKKLPR

Macromolecule #38: bL36m

• Macromolecule: Name: bL36m / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 5.49574 KDa

Sequence

String:

MKVRGKVKLF CDGCVRTIVR LAKEKHIVLV ECSKNPRHKQ RSKFAR(UNK)

Macromolecule #39: mL40

• Macromolecule: Name: mL40 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 10.89544 KDa

Sequence

String:

EGNTRLQKVV SFFVPEVEKK EEEEKLATQY KRWKVAQVHA WNHDIAVKHR LQTEAIASLP QRLKEQALKP DYSPIPLNRK LLFHTPPES YRD

Macromolecule #40: mL41

• Macromolecule: Name: mL41 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 10.819591 KDa

Sequence

String:

VRSKVYQIFL KNAPTREEVL KKVYEHAQQQ QGLRKGWQVK AASWVKKIHV DRGDVKVGLR GRDGQFHVID DLLPKYVVPD LKNFELKPY VALS

Macromolecule #41: mL43

• Macromolecule: Name: mL43 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 14.180325 KDa

Sequence

String:

AKYGTHMLES LVFKYCDIGG SSRGMRLFLK DYMDPFKQTN PQLRIEEVQN RRRHPMLVAL YRNGQCKPVC VRNLSPEEIA KHIFWLRNS HGRDDDYKVP RSHKVVRNES IQGTWAPQGP TL

Macromolecule #42: mL46

• Macromolecule: Name: mL46 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 20.078188 KDa

Sequence

String:

RAYVSCVLER LPIIFQPEPP KELLGLEKHL YETGQIKEYP TVTAADKSGN NKTMKRMLNE RLFLLLKIKG ASGKDIWSFP TLKNTETES LRDTCERSLY TAIGKQYPIF FVGNSPMGHL SKPGGKMFFL AAQVLEDPWE VRLTPESGAE DYAWVTKSEL K EFISDNRA LELFSKML

Macromolecule #43: mL63

• Macromolecule: Name: mL63 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 7.181428 KDa

Sequence

String:

VVFKTTGGKA WNPPGGLKPL TNTQKRSRKE NLQILLRNLS VLKLAAENQP EVTVNLFSPL KFMH

Macromolecule #44: mL64

• Macromolecule: Name: mL64 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 14.227712 KDa

Sequence

String:

AHYLQRFGEA ALPPLVPFSE ALKIREEAYK LGQVWPFEHV VPGVPKAPNA TAYLERKKQK EEKRTKRAKE INDALAKMPQ LIADYKAAR KIDWAEVSII DKLTLSKKQI REKYVKRRLM KQN

Macromolecule #45: mL87

• Macromolecule: Name: mL87 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 16.437799 KDa

Sequence

String:

RPIMHKNWDW EFVVGAKAGR KPAIQRPKPH QWYYCNPKYS AEDPLPTKIF PPHAPPTAES LDDWAKFRKL CPKDPVEAKK FRKHFVRFL NQRNYDWRTA FERGLAKEVA VAKAAQRAED ETKRQEAWHA YRTAVFESAL

Macromolecule #46: mL116

• Macromolecule: Name: mL116 / type: protein_or_peptide / ID: 46 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 46.617367 KDa

Sequence

String:

GTIFNTGVPG PRPEVAQKLS TEYQGHILRM ISLAESASEL DEVLWSSKKH LRPVHIARSC LKLEYLRTKE KGREVSEPIK NLASELENY VELYSTKFTI GQVSQLVRGL SSIRRNIQPD LLLKLAAVVV ADDGRQVQLA NEMDCRDLFF GFFSQGFDNE L FWKRLSES VLPRLPYFNA DVVSTVLRVV SGLRFLHNTE FAHATMTALV PKVGDLSPAR LADAFFSASL LDPTDVSGLN AK LEERFLR EFTSFPIKDT VTMFQTVTVR RHSTPELAAQ VAPLVAAQAH QLPVRHLRRA LEGMVTAGWK DTAEIPLYAI LAK QAARLV LGKQSAATSA ILGKHVDNQG YQRTPVQLLR QLARIFANTG LKAGPGANQP LAPYFAALQR ELEGRLAELD EQVT DDFAE SFKKVGIAEG ARVQI

Macromolecule #47: mL118

• Macromolecule: Name: mL118 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 40.870789 KDa

Sequence

String:

YTWHFLSRQR VEAVNKATDI LELEDIMRLE GNKYDYIAIR AFLKRVCILL QERADALGLP PSNEGLLVRF DEPERARYEA LVSQVCDVV SARAKWFDPS NAAAVAYCLT RWLGRAEAPL IEQLLRRVVA RLPEAKSKDV QYALDATLES AAAPHLEHLR E PMLRAAGA FLGAKLPTGR VPPEVVAKIT RLLVNHWDQP DEELLEAIVT DIAVRLEIYS PTALGRTLLA LSKVPALTGA AF KRSRSSF LPEGVNVPSG ADVAVPLADA CLAHVAAHAA EHANEHDLIK FLGAISKLAS PGRAATAGAD AGAEATESGA AWA KRNSAS LAWFALEQRL APSTRGSFEG NQFPFVIKLV SAAARPPPAV TKFISSTVAK E

Macromolecule #48: mL120

• Macromolecule: Name: mL120 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 22.283516 KDa

Sequence

String:

IEEYYVVPPA CPPPPHNPKT LKYVPKLNRT QIIARVYEAK TPTELENSAL GKKFFSEFAA VAKLVRLSQL RRANVYNSRD DAMCVSIYN TSVRVADKLL HLSSDEELCG LIWALSQLPY PEYENLVDRS LQILLEEDKP LKTGSSLAVS RAAAGLASLG R WDASTWEV LVPLLRKNVQ EGKEVELSNL ALGLYDARET V

Macromolecule #49: mL121

• Macromolecule: Name: mL121 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 27.805775 KDa

Sequence

String:

ITTLEFYEQN KEKLSFLVGN DVYSEFEEKW KPKPKVFDPE IPIAEAMWPE GRSEKLQSVI VKKLSFSTES AGSVVAGMKH GYCPDSVDV IVSRVEQLKA FEGYFPGFKT EEFVSVNPRL LNFSTDRIYW AMLTFQDMFS SAEVGPLMAN IGHFIIENPI R CAKNLFCL ATELESQLSM KLDVTAVKQD SWFTLSSEET IRERVAALAT IFGSKTAGDI LLRDINYLRL DSKDVNRDAV KI REHF

Macromolecule #50: mL122

• Macromolecule: Name: mL122 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 7.019003 KDa

Sequence

String:

RKNIVWPEQL EEQKRNKQYE YQWVISNGKK FIARRESTST KWEIWKSIEK VTPGQKP

Macromolecule #51: mL123

• Macromolecule: Name: mL123 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 45.953566 KDa

Sequence

String:

YRNKVTIEYI KLKEPENDDY ATRDPTNYAQ LLGAISISRH LDRTTYLYET FKDKFDTIHY VTALTKLPGL VHYRGADLVM RDGVQWSEG VKPFWQKPNA QPRKHLLPKA QGLLSKLEEQ FPPHLNNLFP RQTANLIWAY GQLKRKQVVA ACPFLGDFLL S LRRDNFLA LDKHATGADY AQIVKGLANL QTAGSPADED TRALIEDFVD QLTQEMLLRR GHARLLDARE AQSILWGLGK LN RRKNTAI IDVLCDVVLA GVNSLTPTAL AGAFSALAKL GHSSRTDVFE AMAKGYHLQT TLMSPQDVSL TVCACADLGF RDD NLLKIC GLKAADMLGE FSNASLAWLM AGFGRLGYNH EAFFSAVNKS VLAEPVVEVE PGFAWRVLSA YAGSGRKDSE SLKV CGRIT EAFLAKLY

Macromolecule #52: mL124

• Macromolecule: Name: mL124 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 52.21218 KDa

Sequence

String:

FHTGVNLVQP IDTSKLTRQI KKLTLLHEAA LTVLQYSNYC NPEQATEILR RLPFLMRHEE SRVLKGQTLD PKLPPMFHGL LHVMGDRFV QVFSDCNLRQ IERGAWALAA ARHQHDGVAL ALSEKLKQLT QELLDLNAKP FNTRVTKPTP EQLNSGIFAS R VLVPESVN QLPVKAVLPE FNALAGIAWA LATVAGEHSA AAAKAALEQL AEKFGALQVD PKPLPDADSL CRLAWAFAKA GV HNPAAVD KLFHLAEERL KSQLQAHDPA SGPLRPRCTY RYKTVRGWVD QHFPRKPRDS SYLGDTAPKI IPRDFEIDSL GSL LSAAAL LRDQVPVERL QTILNLAAQH TAASSVAGGA LQPLMVTYEE VTRVLAACEQ LGFRSSTLVT PLLHGLPMAA LSAE ALSQL AAAATLHHVR SRTVYLRIVR AFNAKLSVSP TLVAGAGIGA EGKKEGEAAA ALGAQLLLAV TKAGLPANAS VSRIA SLV

Macromolecule #53: mL125

• Macromolecule: Name: mL125 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 22.855125 KDa

Sequence

String:

RPALTPSFSR VSDPWTGEKE AKYAAPYRIP EEVWKNSGAP KILFQDPWNS PDYDEVRKKH AVLVHDYLKQ QSQPINVQTI LEGVNKTHG LVLGTIEYVT SLLENMLWHD MAYVVKPVFS SPRKAKLSKI PLLYGANKYQ QVFRGTPKEV AERYEAARAK H IKVAFTRL RTSKTPQPFR RRTDEYSHVQ ASQSALGLAA AAA

Macromolecule #54: mL126

• Macromolecule: Name: mL126 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 44.063215 KDa

Sequence

String:

SVKYIPNHAA TPNKYKDAQQ KVLWDRAKKL GKKPEYKVPN IKDTQTVFEI GKLTKLCLEH WKPMHFAAAL GHVINVWTTQ ALKSGRYGG KSFTVRELLG FRSLPYGVNS ITAVLPLQSP EDFLSQPLAK QPFSFKPVSV REEVKKIIAS NPGLLIHNWS L KIEGQPNH PITDEDRAAA VIAICTSSFR ARFNEAGDVA VALVLSRLAR CGYWLPPLYE LIAPFAAFQG ARIDHSSPAV IA NVLLVLA RAKGQAEMGQ PTALQIRAIA PALEQKCLQR LGELLPSLEA LVISDTLAAT ALLSSPEARA LLAQIKAEVL ARN FLGFES RDIIACFKEL VANVYQPLQL SADLPAPGEL RDELPGGEKV LDEQLLAALS GAVVEGGAL(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #55: mL127

• Macromolecule: Name: mL127 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 16.291125 KDa

Sequence

String:

HYPSNPENVI RPLTPRELGN LQQPHNEKLV KHQYKLLRMC KLVPLEERKQ LPLTKEQAKK LWDRAEELAH LDALPEVIVG KFGLTANLI RNVDFALKGR KLVRVKIGST APSLSPKFVA FVLENTLDCA TVRTKGYTVT IFRG

Macromolecule #56: mL128

• Macromolecule: Name: mL128 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 2.949474 KDa

Sequence

String:

KFQSRAEKKY RIMDEKVGKP RFQA

Macromolecule #57: mL129

• Macromolecule: Name: mL129 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 8.260157 KDa

Sequence

String:

AKRLLREAPP FTEQVDEKNF DDKDYLGAGA MSNEQLRKEL EKVEPGEAKW DEESPLIPPP QPRQYRNKGH Q

Macromolecule #62: bS1m

• Macromolecule: Name: bS1m / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 27.631986 KDa

Sequence

String:

VSLPMTKAEL RASLLYKMRK DTLTKVIDSS LSTSVLTIEE KEKIDRTLYA HIETENNHID PGLHCLIRAL RRSNLTMPIL KGQQIQAKV IQKTDEVMLL DPGFYNLSEV PVNYLTTAHI VRKVDDSPRE NLYDVRPGDV VKVLVDDVYT PYGDMQLDVP Q QDPRLILN QVWDELHLKM KKKELVRGRI LNECKSGYAV GVAGFVALLP YANTSREVAN RVGEAQSFQI KSMSEPHRRR IV LQ

Macromolecule #63: uS2m

• Macromolecule: Name: uS2m / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 26.62576 KDa

Sequence

String:

GASLNISELM KVLLQPLPLG KVRNSSLNDV VVEVENFWYI NPEKTLASLA RSLYIIKDVL EQDGHILIVN SNPKLKPLVR ESAYCCLNS NVWFYHDEWI PGLLTKPELY PTLFNKQRPL LDILDKHRLL AKVRGPKGRE SLDILKSVLN AEAESARLPV P NSATGALS KLALVICLDP TYNAEVLREA YFKSLLTISL VNSHSDLSSV TYPVYASDTH IGFQHFFLEW ILRVSNLD

Macromolecule #64: uS3m

• Macromolecule: Name: uS3m / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 32.378928 KDa

Sequence

String:

NNPNPPQIRL LDLVVQRERL RPKNPRDIEL LSAEQTDLAK TLITPPTEEG AEPPAAPQLA GLKQVGLPLN QRDVVSVLHQ SLSNAVGQN VHFRPFFFSN LFQSAPAVAQ YVAHALETGS AWNRVERFFV SSVEGDPNLL GMQVQVKGRL GTKAGKGMKK H WKYGDLDI FTIHDYVDYG RATAFTRMGA IGVRVWLKYK PEAVKDVYFQ RQTNFTMPLS KLLSMPRPPL PLSVDGATSS CW WTRPAPL QPPENLTEQS FASGCAGYDP ATRKLRDPQE IKALLEELDR RE

Macromolecule #65: uS4m

• Macromolecule: Name: uS4m / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 25.534645 KDa

Sequence

String:

VYRNSHTKRF SCRNSRLKYP FEQIWPTLQL SEDDAAMFRR NSRQFVVDLS QNITLKNKFA LNKATKIPHQ DARALIYESN LHLDAVGEH PRYLKIRRVT SQYGSKLMNV RKLRLLLGFK RRNFVQKLYE HTLLQQGSNK SWKVLCALEA KMNLAVTRMG V AEDVLEAT HDLRQNNVTL NGSPAQMKHA RYLEPADVLA VSAVSLIKQR ICKGLFEVAP NTL

Macromolecule #66: uS5m

• Macromolecule: Name: uS5m / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 25.956705 KDa

Sequence

String:

INMDQYLRMD NQDTLELLVQ AKDNFLKKDE RTIPIPQSVR EVMYRHRWDK VIVDVRRLVY RTKNNKREQY TAMVASGNQK GLLGLGMGS AETAQLAVAR AYLDSFSNLA AVPLYRGHTV YHHVEHHFQR VKVTLSPRTS GWGLRCSNLF YELCSLAGIR D LTVDVSGA TRNKFFVAKA FFDALQKQTA PHDGVEGTGV YVKEVVRRPV VNLGTYRNNG TLPPKEYLAT

Macromolecule #67: uS6m

• Macromolecule: Name: uS6m / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 13.776003 KDa

Sequence

String:

PLYELLCLAK PLGKAEIAGA MKIVGQTVLK RGGFITEVQS YGEQQLAYDI RRPFEIYPSA HIFKMEFAAP TDIVRLVTHE MRVNENVLR WVVVRRPRFK HTLQELLEEV KAQENDDFKA

Macromolecule #68: uS7m

• Macromolecule: Name: uS7m / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 12.740046 KDa

Sequence

String:

KLREDNSGKD IKDVIDIVLQ AVDNVKPLMQ VDSNKTGTKV MHVPRPLKPR NQTQFAVKWI IEAAIKRCMA SKRPMHECLA VEILLAYQK KGAARTKRDE LHKLAMDNRA NIA

Macromolecule #69: uS8m

• Macromolecule: Name: uS8m / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 43.012633 KDa

Sequence

String:

AAQAYFDLRY HVKKQGLLTV NRAASIINSI FPEFSHESHR NQLAVPLPRK EIPTYIMQNA KVQPWALLPT KAAAYAQYPN FFRSSSLFF GSLNREIVNR RPYSLLPADK LSMDLAQVCT NLGILNGWDI VQKREKLKDL DFVWPANELP RDHHEVKLFK H LHLRLALK WEQHKPLWED GSMVKDQREY RDQQQVQQQQ PLPHLPLAPL FGPLPLTVRN LSKASQPVLL YPLQLRELAQ RM PSGLFLL YHHELGVITD AQAFLFDVPV VALAHVGLPV SMAAAVNGAV NRTFRAELGK PLREVTKLKD WSLSATIAAQ VRE RRQQLL ERAEQTKRER KQIQDLVTVR VGKFKAEVDK EDSSLALQDE LLAWQLKE

Macromolecule #70: uS9m

• Macromolecule: Name: uS9m / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 32.274049 KDa

Sequence

String:

SWQEHTVENF SIKPSYLSPL FATYQTETEK DDRRRLLLQL LKAHKLLSHE VEGLDSEQIQ RGTRYRIAQM KELASDLRLL LKGDNQPIN FTTCTDKSLM YGFYNLLESE TSVWEHWASH FVEKYKGTPG LEPLEVIFSR QDPMLILEQA GACMTLDGVA E TIGKRKSA LAKVLMRRGT GKIIVNGLPY DAYFRDVEVR ALMLQPFFAC DGLGRFDVEV EVEGSGPSAQ AQAIRTGIAR AL KLHYPDV PNADLEKLSI WDGRKVERKK PGRHKARRGF QWVKR

Macromolecule #71: uS10m

• Macromolecule: Name: uS10m / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 45.092219 KDa

Sequence

String:

NPTDSFYEIE LTVKAYEERY VDMAVNALRD LLMISFTPKK FSPMGQGRYA KDIEPNNPID LYIPTTMERV KVDWKKTRFT LIRGPFVDK RGMEQFERRE YHSKIKASTT SLTELQWLLD ALKLYEFTGV QIEAEVTSPG FVAAHEHQAV LKTSRPTHGE A GDFVDSLF LDDQSSILDA GHLRHIKDFV PSGFGSEMQT ALAALRNVMH QGLEERRRAL GMNSGYDAWL RQQQRVGSAT VT KLFPASG LASSSSLLDE AATPADLSTL LLKSQIDSAA AVRDRKVAAF LAAVDAVFLN LRFDALEGHA RFPFHFATAV PGQ MKVPVA MWMQAVSKMA EYQRQVSEAS QAADLLKAYT SYSAFSQALL YKLMQLWFET ASSDAKEYLA LPSWEEYEAM VQAK R

Macromolecule #72: uS11m

• Macromolecule: Name: uS11m / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 12.426539 KDa

Sequence

String:

VLQLEGVVHI LNTSNNIVLT LTDKEGRVKA WTTPSSVGYK NAKKRLPVAA ETAAGELAKR ALALGYSTVA VKIKGIGTMT QYAVQMLSS SGIKITQLVD VTPVPYGGCR PSRKRRV

Macromolecule #73: uS12m

• Macromolecule: Name: uS12m / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 13.729266 KDa

Sequence

String:

LTVGQLLRGG RTKKVRRSKS PALEQCPYKK GICLRVYTTA PKKPNSANRK VCKVQLSNGY KVLAYIPGEG HNLQEHSIVL VRGGRTKDL PGVKYKVIRG KYDCAGVKGR KTSRSKYGVK RPKE

Macromolecule #74: uS13m

• Macromolecule: Name: uS13m / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 12.807903 KDa

Sequence

String:

VQIQRVTLPP HQCVYMALKK VFGLGGPTSL AVVEACGISK GVRVRDLKEN HVQQITQFIQ DNFVTEDNLR RKVREDIVKL VNIKSRDGL RHDWGVSIKG HTSCNGKTAK RLRH

Macromolecule #75: uS14m

• Macromolecule: Name: uS14m / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 13.921226 KDa

Sequence

String:

TTKNFRLRFR IPQYPDIVGS RRIDKDQTRR VLYDQHEVDR RLYKAMTRDE SLTLPVRLQV QRLFETEMPR DSAPSRIVDR CVLTGRVRG LVKFAGLSRI MLRQLIHYGH IGGVAKASW

Macromolecule #76: uS15m

• Macromolecule: Name: uS15m / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 18.048543 KDa

Sequence

String:

QNAKAHLVEK FLGPEFRPRI KQLKAKRLEA VNDIKRHEAD VSSPEVRVSL MTYRIRDSIP LFDECKKELT GMRKLEIMFN KRRRILRGL RKHNFPIYSM IINKLGMRDI YSDPG(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

Macromolecule #77: bS16m

• Macromolecule: Name: bS16m / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 14.298446 KDa

Sequence

String:

MNNLLTQLPK HDLLKIKLVR WGFNKKPFYV LSVMKRRQAR NSGNRYEDIG WWDPSPHQDG NSHFGIKFDR VKYWLSQGAV PTEKVYGLF GQAGIIPQKP APPKYKPADG NADTKWRPEL QKNL

Macromolecule #78: uS17m

• Macromolecule: Name: uS17m / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 15.002346 KDa

Sequence

String:

GGFEFVGKVV SNRMQKGVVV AVNYVVWIPK YKVYERRTAR HMAHDEGNSC VIGDIVRIQQ HRKLSKNKAY VVVSTLKHAD VYNAKQAAV LNTERSQNKL GRVEYAESRL SEVQQRLLKL REMYQKELAL N

Macromolecule #79: bS18m

• Macromolecule: Name: bS18m / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 10.606415 KDa

Sequence

String:

FRPRSTHQEV TEMADFKNVS FLTQFLSPAG RIQPRRVTKL TEPLQRRIAK SVKLARNMAL MAGEARLDKL HLTRVRQEEL HRYQLKKSV Q

Macromolecule #80: bS19m

• Macromolecule: Name: bS19m / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 10.38481 KDa

Sequence

String:

GRAVWRGPYV SVSLLEQVIT FAKRNPEWWN IGRFQGSKAP EAINTFCRSS TVLPDFLGLK FGVHNGREFV GVEVQEAMVG HKLGEFSQT TFR

Macromolecule #81: bS21m

• Macromolecule: Name: bS21m / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 8.829331 KDa

Sequence

String:

AFVAEVRDGN VDAALAQVKK YRKTEGVNDS VRKKEYFLRP SLQKFRDSEL TYAKNMGKLI HERVNWVVKR KKIKL

Macromolecule #82: mS23

• Macromolecule: Name: mS23 / type: protein_or_peptide / ID: 82 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 19.393281 KDa

Sequence

String:

AFSRYFVQKF KQSYTRKYMR DMESGAFSFP KCHDILGKYR PDVLFAAAAA PLKLELPEQA VYKKLYRDFP ELRKDAVDLS SLEAPLAKQ FALKHLVLSA EIAANSPRTR HILRRDLEAD PAYERLKEEF MPRIAELRKQ QEQTASLQQL QADEEEHLKL A LTYVAAQ

Macromolecule #83: mS26

• Macromolecule: Name: mS26 / type: protein_or_peptide / ID: 83 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 19.292008 KDa

Sequence

String:

RVFTSNKMDE ERAKRITEQR DFIQARVEWK KATISLYKEW RRTFLQKDIE EQHTKEVAAE QAKVAFAEQK AAAEHVRRQA ALEELLRKT EIECQLAMVR VGRAQKAEET GRLIKKAENL RYAMLLEESR NWIDSEEELE KRINQSLENP EAFGFIVDLP I AKGL

Macromolecule #84: mS29

• Macromolecule: Name: mS29 / type: protein_or_peptide / ID: 84 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 39.111852 KDa

Sequence

String:

SKILVEKRSP ELTQEHIGNY YKVTTERVPE GFMPFHQAFY AKPDAGQERK GGCRGIQHEF DISGHHNVML RSSTLELFDL IKEGDKNRI LLSGPTGTGK SVALFSLVEW ARQQDWIVLY IPSAFTLTRG GFFYRRPGTD LFDTLTSAQH LLKGLLDCHQ A QLAKLPLS SDDSKLLELV QKGLLNDDAH TAVDCCLEVV KELSLAAATQ PVLFAIDGYN ALFQHTDYGV TEGDIQVARR RL LKVEELT LANSMRLLER ADLGKARVVV APSWSIRSSL QVGKPVETTE FVMPRFDFAE TANALYYYQC CGLAPDVPTE KQA KLMQHI TNGNAFEIRS LAIKMSMLKL NKL

Macromolecule #85: mS31

• Macromolecule: Name: mS31 / type: protein_or_peptide / ID: 85 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 66.912 KDa

Sequence

String:

SSAARWRAAI AQRLGVEAAA AAQALAALLG QGDLALTVLA AASEADVLNI TELLENNSVD EAVTNARKVA IVSGHGLFLA TATSEDLAA LSDVEAGELA ALMGKVHVVG LPLADALLGS DSLTHDQLLT LTRSEKQALL WRLASVGKLR EGRAKAVAAL R KAALDRAA AAAEASEGLL SAAAMMKLEH DIAEFDLVRE RYLPGPGLPE GVQEAFAPSG LPSAFSRDEQ ALYDAYFGLR SH AASAQPE PLEGPSAAQL HSSFLDGFQC REEDSQMEEL PESFGQWVAN IKGLIVKAPV PLLGLLAKFV TAKIDGADAR DAS ETQSRL RLLAAEIATD IARRREARLA VSPWWQRASA PIDALAISSI DHPSSDPLVQ LLEVLLGHSG ADEFGSWISA VAMR PVSPY EILADEHRLM DLERYLSMTS ASELHLELAA TPLPWASPAV HVPPAAFLEE MRAKFNNYLL ATGLSPLSAA EWSAY KDWA LEEFAEKRAL GEEALLQEGH SGFFNPKADE IYLRALLEAT IPPEAPLREQ AVRYLETVNM NKTWTFLKKK HMVQRL AEL SRHLTEHPPV EEQGSPFAAL FAVGPGAKPT PLVPKLSKRL PAHGPESLDL PELPEIFR

Macromolecule #86: mS33

• Macromolecule: Name: mS33 / type: protein_or_peptide / ID: 86 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 9.192518 KDa

Sequence

String:

SYIPPSEVPE EHLKNIYEGI FGSPVAEFER TGRCALAKPL IGTKLSSYYF LPPNRQPGFH NEEHEYAKVR LMSKRQKK (UNK) (UNK)

Macromolecule #87: mS34

• Macromolecule: Name: mS34 / type: protein_or_peptide / ID: 87 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 14.053026 KDa

Sequence

String:

SYLAQLVPRD KRTLSNVILN LPNHGIGGIF ARDTWHPESK RYWEILEVVT AKDSSAKVDA YGYLYYQGEK THPFPKRVHS VWRYGWYWK PKPEQEKLLQ PLKERYNTLV DMGIIDPSQL

Macromolecule #88: mS35

• Macromolecule: Name: mS35 / type: protein_or_peptide / ID: 88 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 19.588945 KDa

Sequence

String:

QASATQWKAG VEALRQANPD VLATWIGTTG SEACMEAATA RPPYAGARPL LRWRQTTILH AGSAFMVHPR NAPVQLDVRV SDLKQELGL SDDSAALLTR LAGPERLDEK TKRRVSPCWY NSEEDTLSIA ADRYNDRESN RRLALEQLQA LVKECDTRYP G ETTPAKQY LSGFGFFQ

Macromolecule #89: mS37

• Macromolecule: Name: mS37 / type: protein_or_peptide / ID: 89 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 9.493043 KDa

Sequence

String:

PWQLKEPDNS RVVKNLVKPS KPCGKELLAL IDCTRRNFSG DADGTCMRER AALARCSSQR KKTSKGEILN HLKNLAASWK RFGF

Macromolecule #90: mS38

• Macromolecule: Name: mS38 / type: protein_or_peptide / ID: 90 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 4.004099 KDa

Sequence

String:

ITTMQWRKLK IKKHKLRKRR KANRMRVVKV R

Macromolecule #91: mS45

• Macromolecule: Name: mS45 / type: protein_or_peptide / ID: 91 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 30.779939 KDa

Sequence

String:

PSVNDLASLL SLSEQYRGAD VLAEGAALPG TGFANARGTF LPHELPTAIE YLKELDPEAE MKLEQMEAMY KLLYSRNESE REVGRQMMY DLLKLSGHPF RELELCNWDY MAAFLDARVA GRVFHRGSGE RLVHRTATFP AFEGYPLAEV DQTTEGEVSK L NREESKRQ DNAMFQDFRK KLLFNLGMVG EQLWEPVQGV LSANLRSALD RPLVVYDITA ATGETVYPPK FVAEVDGTRR AL NEQERAY QAKRKPGPRL PYYMRRIARK EEL

Macromolecule #92: mS106

• Macromolecule: Name: mS106 / type: protein_or_peptide / ID: 92 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 18.551984 KDa

Sequence

String:

IAPSQLDKLE KFVHVRPPKT DYEEDIKQAI SSVTDNEGLK KCLDLFLTNH AAQTWVGKHE YANAGQVSDF IKVCEKVGSS APLVTLWQS AYRYGVDPTV PLLRSSAAAC AALGEGADAA LVLLYGSCYI VNVPEDLAAA VRTALEAHEK AQEGNAEALA K VQKYREAL DRL

Macromolecule #93: mS107

• Macromolecule: Name: mS107 / type: protein_or_peptide / ID: 93 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 42.316926 KDa

Sequence

String:

MATIPKGLDI DPESPMLYHY FKSIHPHQVS FRIKKRKQLQ HLWELCKLYE NKMDTLASAA MLGQLFRLQK RNNPDYSVEL ANQIFEHCV KRLSFTIRFA TYQEIVPVLF TLARMNVSIV PSDTLLLDPT HRVSREFVHL FLKRAVRNHV HIRVVNPRQM A RVLWATAK LFPEDQRMDP RVQDAVDKLA RSSVKRLSEL HPGSLSIYAS AFAKLSPAPT SQEGPLKDVD VSSWDATITG VK SSLLDLD SKELAFVARA RTLKVFQGIS REILLRVGDL NHEQFTVRNV FHVLGAYIRA QIQDPLVAKV LAENITGRIQ DVY AEELIA LVRAAERLDG FKNPDLTAAV LRRAREVDLP EETQKDYAKR LQSA

Macromolecule #94: uS4m-2 (fragment)

• Macromolecule: Name: uS4m-2 (fragment) / type: protein_or_peptide / ID: 94 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 20.395135 KDa

Sequence

String:

ADLVRHLQSS GSKLQKLANL TSASCSYRDI SVSLFGLQAR QLGCTKPFVY TFSASEQAQS KSKPFDGKLR LPDVSQLSDT ITVSGDAAP ATLNLDQKYM DKISCWTQTI PSHLEMSYQN LTSVKLFPPV DASYPTYVDF EHATRLLDHF TSRKRLNYQR K MIKKRDKF QIKSWDHHAG EA

Macromolecule #95: mS108

• Macromolecule: Name: mS108 / type: protein_or_peptide / ID: 95 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 5.864713 KDa

Sequence

String:

HRFRNNKFLR LEPDLDPKVY GQTETLQKQV DDNFSLLLAK HRLDMKAAAA

Macromolecule #96: mS109

• Macromolecule: Name: mS109 / type: protein_or_peptide / ID: 96 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 18.394961 KDa

Sequence

String:

NNPEDNIAIR NAPQKVFQLI GDPRSGVADD VTHNPFGEIT VEMVWHQLKN EVYWYRYEFA GQTTEQSHRD SRLSDYTKNL IYLLRAKNP RKWTIEALAE KFHIRKQRVL AILALKEMEA HKIESGKQLQ GPLSPYGLKV RVEDLKLLSL DPTGCGWVEP

Macromolecule #97: mS110

• Macromolecule: Name: mS110 / type: protein_or_peptide / ID: 97 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 11.167972 KDa

Sequence

String:

TTRRRKLLGS RYGARLAKKN RQQFERARVI LDCYSDEELR PDSPPVAVKE VTINTLQTMR FLFPQTSKEH LDIKQNIASY KIFSLNREL LSLLPK

Macromolecule #98: uS3m-2 (fragment)

• Macromolecule: Name: uS3m-2 (fragment) / type: protein_or_peptide / ID: 98 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 12.220075 KDa

Sequence

String:

GRHLLPATAI RVRLNRGFES AWYTDVSYRE MIKKDFLLAK LASSFVNRSS RASLRQIFPG GKDFPNFRTS RIFMQHLPYK SYASTFSYV APKDGPQAKY GLFQSKL

Macromolecule #99: mS111

• Macromolecule: Name: mS111 / type: protein_or_peptide / ID: 99 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 17.507816 KDa

Sequence

String:

PFNVSDANPK DVEFLQVLLS KFLPDADKAT VYRTGQEPRR LRLGDLPATS QFMESFVSEK LPKEPLYDMP SWLANNMPQY DAQPKSPHY HWSSWMRQHL SLDLQRLYAA FAEYMASEPH RLGIVRQANF ELARLWDWQH RRVAAGLSPD L

Macromolecule #100: mS112

• Macromolecule: Name: mS112 / type: protein_or_peptide / ID: 100 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 7.917274 KDa

Sequence

String:

SADVYKEFFK MARVAVRTMK EPTKSIMKDL QRNARRSENI QRDNNLDKGV YMRFLRQRAG LSVPPIK

Macromolecule #101: mS113

• Macromolecule: Name: mS113 / type: protein_or_peptide / ID: 101 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 7.505579 KDa

Sequence

String:

GADGVLRRHN EVRGALRFFL DSWYKNKTSG TIADKNQVMF DYLKYKGVTE IAQHLHAPAP QVFRK

Macromolecule #102: mS114

• Macromolecule: Name: mS114 / type: protein_or_peptide / ID: 102 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 15.270999 KDa

Sequence

String:

AHYLQRFGEA ALKTRSVGGK ILPPLVPFSE ALKIREEAYK LGQVWPFEHV VPGVPKAPNA TAYLERKKQK EEKRTKRAKE INDALAKMP QLIADYKAAR KIDWAEVSII DKLTLSKKQI REKYVKRRLM KQN

Macromolecule #103: mS115

• Macromolecule: Name: mS115 / type: protein_or_peptide / ID: 103 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 40.611492 KDa

Sequence

String:

EYNGQGYVFS LLQRPPAPTL ELLAEYLTVK YQDVIAQRDF VTHILGRMSV LERGGELPAA DAAASGTWTG GAKRRLSPQE IRDINGELN RLFDADLNEY VSLAQRLATE NVLSPADLAT CLQAARSKAQ TSSFASLAAP GSSNVDRNIL AQVLQGKQDV S ALAAAAAA AAASGPEGAR VAWDEALQVG KYGAWATKAK AWAADDIAAR REKGQQISPE QEAALVCLWD NPLSYDAAAG LW HQYAEKA GAVSAPSLAD VISADQAIQA AKAAAAADPA SLPAVKATAE KAAQVQEAVK KLYLGFAARQ GSTSGAVTVD GVP LPFADV VKANAELDVA SPAALAAAFQ PLELGELLAC HWEAVSRTFM WEDMYQLMLE TAKEIEVNGA

Macromolecule #104: mS116

• Macromolecule: Name: mS116 / type: protein_or_peptide / ID: 104 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 10.018448 KDa

Sequence

String:

GPLPEDVFLV APKVAAAVQQ TQAQLIDLLA PYGYSFDAFS EAVLEDLSKT KELCVKARFV LWEARVLEAL EAVRPFVSGP VFRTESEAA ALT

Macromolecule #105: uS7m-2 (fragment)

• Macromolecule: Name: uS7m-2 (fragment) / type: protein_or_peptide / ID: 105 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 9.622189 KDa

Sequence

String:

TVVLAPSKYD SQLKIPLKPT EMDEFEELRS FVDISIEKEA DYVMNKFVGR LIKGGEKATA QQVLLRTLLH TRRLMQEGNI TSLK

Macromolecule #106: Unknown

• Macromolecule: Name: Unknown / type: protein_or_peptide / ID: 106 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 2.813459 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)R(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

Macromolecule #107: mL105

• Macromolecule: Name: mL105 / type: protein_or_peptide / ID: 107 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 15.669843 KDa

Sequence

String:

RDEIIKLLES RKDMDVNGYV MYCREELGKL TVPRPRAPPV SPKHEDYKTF VDEERVTYMR MKQHEKISLF LTEEEKNTVT TKGKDILDD KRFIQTIASR TGFYIAEEVR DCLSEFFNFR DSSRRLLTYY AD

Macromolecule #108: Unknown

• Macromolecule: Name: Unknown / type: protein_or_peptide / ID: 108 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 3.677524 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #109: Unknown

• Macromolecule: Name: Unknown / type: protein_or_peptide / ID: 109 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 4.017944 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #110: Unknown

• Macromolecule: Name: Unknown / type: protein_or_peptide / ID: 110 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 6.911511 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)

Macromolecule #111: Unknown

• Macromolecule: Name: Unknown / type: protein_or_peptide / ID: 111 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 6.145567 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #112: Unknown

• Macromolecule: Name: Unknown / type: protein_or_peptide / ID: 112 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 4.103049 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #113: Unknown

• Macromolecule: Name: Unknown / type: protein_or_peptide / ID: 113 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 2.230741 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #114: Unknown

• Macromolecule: Name: Unknown / type: protein_or_peptide / ID: 114 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 783.958 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #115: Unknown

• Macromolecule: Name: Unknown / type: protein_or_peptide / ID: 115 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 1.975426 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

Macromolecule #116: Unknown

• Macromolecule: Name: Unknown / type: protein_or_peptide / ID: 116 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 1.379692 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #117: Unknown

• Macromolecule: Name: Unknown / type: protein_or_peptide / ID: 117 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Polytomella magna (plant)
• Molecular weight: Theoretical: 954.168 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

Macromolecule #118: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 118 / Number of copies: 112 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #119: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 119 / Number of copies: 31 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Macromolecule #120: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 120 / Number of copies: 1 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #121: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 121 / Number of copies: 1 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

Macromolecule #122: water

• Macromolecule: Name: water / type: ligand / ID: 122 / Number of copies: 504 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER / Water

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

Buffer

pH: 7.45
Component:

Concentration	Formula	Name
25.0 mM	C8H18N2O4S	HEPES
5.0 mM	MgCl2	Magnesium Chloride
50.0 mM	KCl	Potassium Chloride

• Grid: Model: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2.0 nm / Pretreatment - Type: GLOW DISCHARGE
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 298 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: C2 aperture diameter: 150.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.4 µm / Nominal magnification: 130000
• Specialist optics: Energy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 45850 / Average exposure time: 1.5 sec. / Average electron dose: 30.0 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 2999000
• CTF correction: Software - Name: Gctf (ver. 1.06)

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

6z1p

• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0.1)
• Final 3D classification: Number classes: 3 / Software - Name: RELION (ver. 3.0.1)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0.1)
• Final reconstruction: Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 2.91 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0.1) / Number images used: 184300

Atomic model buiding 1

• Refinement: Protocol: OTHER

Output model

PDB-8a22:
Structure of the mitochondrial ribosome from Polytomella magna