ジャーナル: Structure / 年: 2008 タイトル: The 3D structure of villin as an unusual F-Actin crosslinker. 著者: Cheri M Hampton / Jun Liu / Dianne W Taylor / David J DeRosier / Kenneth A Taylor / 要旨: Villin is an F-actin nucleating, crosslinking, severing, and capping protein within the gelsolin superfamily. We have used electron tomography of 2D arrays of villin-crosslinked F-actin to generate ...Villin is an F-actin nucleating, crosslinking, severing, and capping protein within the gelsolin superfamily. We have used electron tomography of 2D arrays of villin-crosslinked F-actin to generate 3D images revealing villin's crosslinking structure. In these polar arrays, neighboring filaments are spaced 125.9 +/- 7.1 A apart, offset axially by 17 A, with one villin crosslink per actin crossover. More than 6500 subvolumes containing a single villin crosslink and the neighboring actin filaments were aligned and classified to produce 3D subvolume averages. Placement of a complete villin homology model into the average density reveals that full-length villin binds to different sites on F-actin from those used by other actin-binding proteins and villin's close homolog gelsolin.
ダウンロード / ファイル: emd_1496.map.gz / 形式: CCP4 / 大きさ: 822.5 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Tomogram of F-actin crosslinked by villin
ボクセルのサイズ
X=Y=Z: 5.76 Å
密度
最小 - 最大
-8.51751 - 9.68876
平均 (標準偏差)
0.00000000632091 (±1.0)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
-1000
-920
-30
サイズ
1840
2000
60
Spacing
1840
2000
60
セル
A: 10598.4 Å / B: 11520 Å / C: 345.6 Å α=β=γ: 90 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
5.76
5.76
5.76
M x/y/z
1840
2000
60
origin x/y/z
0.000
0.000
0.000
length x/y/z
10598.400
11520.000
345.600
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-55
-55
-55
NX/NY/NZ
111
111
111
MAP C/R/S
1
2
3
start NC/NR/NS
-920
-1000
-30
NC/NR/NS
2000
1840
60
D min/max/mean
-8.518
9.689
0.000
-
添付データ
-
試料の構成要素
-
全体 : rabbit F-actin cross-linked with chicken villin
全体
名称: rabbit F-actin cross-linked with chicken villin
要素
試料: rabbit F-actin cross-linked with chicken villin
細胞器官・細胞要素: cytoskeleton
細胞器官・細胞要素: cytoskeleton
-
超分子 #1000: rabbit F-actin cross-linked with chicken villin
超分子
名称: rabbit F-actin cross-linked with chicken villin / タイプ: sample / ID: 1000 / 詳細: 2D rafts are formed on a lipid monolayer / Number unique components: 2