- EMDB-1454: Human T-lymphotropic virus-1 visualized at the virological synaps... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-1454
タイトル
Human T-lymphotropic virus-1 visualized at the virological synapse by electron tomography.
マップデータ
The tomogram shows a virological synapse between a chronically HTVL-1 infected MS9 cell (cell line)(top) and a Jurkat cell (bottom). Numerous HTLV-1 particles can be seen within the synaptic cleft.
試料
試料: HTLV-1 virological snapse formed between a chronically HTLV-1 infected MS9 cell and a target cell
ジャーナル: PLoS One / 年: 2008 タイトル: Human T-lymphotropic virus-1 visualized at the virological synapse by electron tomography. 著者: Endre Majorovits / Mohamed Nejmeddine / Yuetsu Tanaka / Graham P Taylor / Stephen D Fuller / Charles R M Bangham / 要旨: Human T-lymphotropic virus 1 (HTLV-1) is transmitted directly between cells via an organized cell-cell contact called a virological synapse (VS). The VS has been studied by light microscopy, but the ...Human T-lymphotropic virus 1 (HTLV-1) is transmitted directly between cells via an organized cell-cell contact called a virological synapse (VS). The VS has been studied by light microscopy, but the ultrastructure of the VS and the nature of the transmitted viral particle have remained unknown. Cell-free enveloped virions of HTLV-1 are undetectable in the serum of individuals infected with the human T-lymphotropic virus 1 (HTLV-1) and during in vitro culture of naturally infected lymphocytes. However, the viral envelope protein is required for infectivity of HTLV-1, suggesting that complete, enveloped HTLV-1 virions are transferred across the synapse. Here, we use electron tomography combined with immunostaining of viral protein to demonstrate the presence of enveloped HTLV-1 particles within the VS formed between naturally infected lymphocytes. We show in 3D that HTLV-1 particles can be detected in multiple synaptic clefts at different locations simultaneously within the same VS. The synaptic clefts are surrounded by the tightly apposed plasma membranes of the two cells. HTLV-1 virions can contact the recipient cell membrane before detaching from the infected cell. The results show that the HTLV-1 virological synapse that forms spontaneously between lymphocytes of HTLV-1 infected individuals allows direct cell-cell transmission of the virus by triggered, directional release of enveloped HTLV-1 particles into confined intercellular spaces.
ダウンロード / ファイル: emd_1454.map.gz / 形式: CCP4 / 大きさ: 166.6 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
The tomogram shows a virological synapse between a chronically HTVL-1 infected MS9 cell (cell line)(top) and a Jurkat cell (bottom). Numerous HTLV-1 particles can be seen within the synaptic cleft.
ボクセルのサイズ
X=Y=Z: 31.4 Å
密度
最小 - 最大
-127.0 - 127.0
平均 (標準偏差)
58.237000000000002 (±9.14335)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
643
1312
212
Spacing
643
1312
212
セル
A: 20190.2 Å / B: 41196.8 Å / C: 6656.8 Å α=β=γ: 90 °
CCP4マップ ヘッダ情報:
mode
envelope stored as signed bytes (from -128 lowest to 127 highest)
Å/pix. X/Y/Z
31.39999844479
31.400000762195
31.4
M x/y/z
643
1312
212
origin x/y/z
0.000
0.000
0.000
length x/y/z
20190.199
41196.801
6656.800
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-60
-60
-59
NX/NY/NZ
120
120
120
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
1312
643
212
D min/max/mean
-127.000
127.000
58.237
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添付データ
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試料の構成要素
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全体 : HTLV-1 virological snapse formed between a chronically HTLV-1 inf...
全体
名称: HTLV-1 virological snapse formed between a chronically HTLV-1 infected MS9 cell and a target cell
要素
試料: HTLV-1 virological snapse formed between a chronically HTLV-1 infected MS9 cell and a target cell
細胞器官・細胞要素: virological synapse
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超分子 #1000: HTLV-1 virological snapse formed between a chronically HTLV-1 inf...
超分子
名称: HTLV-1 virological snapse formed between a chronically HTLV-1 infected MS9 cell and a target cell タイプ: sample / ID: 1000 / Number unique components: 1