- EMDB-14479: Cryo-EM structure of the ribosome-associated RAC complex on the 8... -
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基本情報
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データベース: EMDB / ID: EMD-14479
タイトル
Cryo-EM structure of the ribosome-associated RAC complex on the 80S ribosome - RAC-1 conformation
マップデータ
試料
複合体: Chaetomium thermophilum RAC on 80S ribosome
RNA: x 4種
タンパク質・ペプチド: x 81種
リガンド: x 3種
機能・相同性
機能・相同性情報
RNA polymerase I core factor complex / 'de novo' cotranslational protein folding / dolichyl-diphosphooligosaccharide-protein glycotransferase / dolichyl-diphosphooligosaccharide-protein glycotransferase activity / protein N-linked glycosylation via asparagine / RNA polymerase I core promoter sequence-specific DNA binding / protein kinase regulator activity / nucleolar large rRNA transcription by RNA polymerase I / 90S preribosome / regulation of translational fidelity ...RNA polymerase I core factor complex / 'de novo' cotranslational protein folding / dolichyl-diphosphooligosaccharide-protein glycotransferase / dolichyl-diphosphooligosaccharide-protein glycotransferase activity / protein N-linked glycosylation via asparagine / RNA polymerase I core promoter sequence-specific DNA binding / protein kinase regulator activity / nucleolar large rRNA transcription by RNA polymerase I / 90S preribosome / regulation of translational fidelity / translation regulator activity / Hsp70 protein binding / endomembrane system / post-translational protein modification / cellular response to amino acid starvation / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / methyltransferase activity / maturation of SSU-rRNA / small-subunit processome / ATP-dependent protein folding chaperone / rRNA processing / ribosome biogenesis / large ribosomal subunit / regulation of translation / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / small ribosomal subunit rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / methylation / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / mRNA binding / nucleolus / RNA binding / zinc ion binding / ATP binding / metal ion binding / nucleus / membrane / cytosol / cytoplasm 類似検索 - 分子機能
Ribosome-associated complex head domain / Ribosome-associated complex head domain superfamily / J-protein Zuotin/DnaJC2 / : / Ribosome-associated complex head domain / Zuotin-like, zuotin homology domain / Gcn1, N-terminal / : / : / Generalcontrol nonderepressible 1 (Gcn1) N-terminal ...Ribosome-associated complex head domain / Ribosome-associated complex head domain superfamily / J-protein Zuotin/DnaJC2 / : / Ribosome-associated complex head domain / Zuotin-like, zuotin homology domain / Gcn1, N-terminal / : / : / Generalcontrol nonderepressible 1 (Gcn1) N-terminal / Stalled ribosome sensor GCN1-like, HEAT repeats / Fungal GCN1-like, HEAT repeats / Stalled ribosome sensor GCN1 N-terminal / Transcription initiation factor Rrn7, Zinc-finger / RNA polymerase I transcription initiation factor TAF1B/Rrn7 / : / : / Hyaluronan / mRNA binding family / Zinc-finger of RNA-polymerase I-specific TFIIB, Rrn7 / Rrn7/TAF1B, N-terminal cyclin domain / Rrn7/TAF1B, C-terminal cyclin domain / E-Z type HEAT repeats / PBS lyase HEAT-like repeat / Elongation factor 3/GNC1 HEAT repeat / EF3 N-terminal HEAT repeat / Centrosomal protein CEP104-like, TOG domain / RNA binding protein HABP4/SERBP1 / : / STT3/PglB/AglB core domain / Stm1-like, N-terminal / Stm1 / Parkin co-regulated protein / TOG domain / Parkin co-regulated protein / TOG / : / Oligosaccharyl transferase, STT3 subunit / Oligosaccharyl transferase STT3, N-terminal / Hyaluronan/mRNA-binding protein / Hyaluronan / mRNA binding family / DnaJ domain / HEAT repeat profile. / HEAT, type 2 / DnaJ molecular chaperone homology domain / dnaJ domain profile. / Chaperone J-domain superfamily / DnaJ domain / 60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / Heat shock protein 70 family / Hsp70 protein / : / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L30e / Ribosomal protein L28e / Ribosomal protein L13e / 50S ribosomal protein L10, insertion domain superfamily / : / Ribosomal protein L30e signature 1. / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L30e signature 2. / Ribosomal protein L30e, conserved site / Ribosomal protein L13e / Ribosomal protein L30/YlxQ / : / Ribosomal protein S26e signature. / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein S5, eukaryotic/archaeal / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / S27a-like superfamily / Ribosomal protein L10e / 40S Ribosomal protein S10 / : / Ribosomal protein L44e signature. / Ribosomal protein S7e signature. / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19, eukaryotic / Ribosomal protein L19e signature. 類似検索 - ドメイン・相同性
Large ribosomal subunit protein uL3 / 40S ribosomal protein s23-like protein / 40S ribosomal protein S6 / 40S ribosomal protein S8 / Putative ribosome associated protein / Small ribosomal subunit protein uS2 / 60S ribosomal protein L26-like protein / Ribosomal protein L15 / 40S ribosomal protein s10-like protein / 60S ribosomal protein L27 ...Large ribosomal subunit protein uL3 / 40S ribosomal protein s23-like protein / 40S ribosomal protein S6 / 40S ribosomal protein S8 / Putative ribosome associated protein / Small ribosomal subunit protein uS2 / 60S ribosomal protein L26-like protein / Ribosomal protein L15 / 40S ribosomal protein s10-like protein / 60S ribosomal protein L27 / 40S ribosomal protein S13-like protein / Putative heat shock protein / 40S ribosomal protein s15-like protein / 60S ribosomal protein L6 / Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 / 60S ribosomal protein l21-like protein / 40S ribosomal protein s9-like protein / Ribosomal protein L37 / 40S ribosomal protein S4 / 60S ribosomal protein L14-like protein / Ribosomal protein s27-like protein / 40S ribosomal protein S25 / 40S ribosomal protein s5-like protein / 40S ribosomal protein S26 / 40S ribosomal protein S27a-like protein / 40S ribosomal protein S20-like protein / Hyaluronan/mRNA-binding protein domain-containing protein / 60S ribosomal protein L2-like protein / 60S ribosomal protein L25-like protein / 60S ribosomal protein L43-like protein / 40S ribosomal protein S3 / Putative ribosomal protein / 60S ribosomal protein L32-like protein / Small ribosomal subunit protein eS1 / 40S ribosomal protein S17-like protein / 40S ribosomal protein S7 / Putative ribosomal protein / 60S ribosomal protein L13 / Ribosomal protein L18-like protein / 40S ribosomal protein S28-like protein / Ribosomal protein L19 / Putative guanine nucleotide-binding protein / 60S ribosomal protein L8 / Small ribosomal subunit protein uS5 / 60S ribosomal protein L36 / 40S ribosomal protein S16-like protein / 60S ribosomal protein L44-like protein / 60S ribosomal protein L20 / 60S ribosomal protein l33-like protein / Putative 60S ribosomal protein / 60S ribosomal protein L22-like protein / Large ribosomal subunit protein uL18 / 60S ribosomal protein l30-like protein / 60S ribosomal protein L10-like protein / 40S ribosomal protein S11-like protein / 60S ribosomal protein L4-like protein / 40S ribosomal protein S19-like protein / 60S ribosomal protein l7-like protein / 40S ribosomal protein S14-like protein / Ribosomal protein l34-like protein / 60S ribosomal protein L12-like protein / 60S acidic ribosomal protein P0 / Large ribosomal subunit protein uL22 / 60S ribosomal protein L16-like protein / 40S ribosomal protein S12 / 40S ribosomal protein S22-like protein / Putative 60S ribosomal protein / Large ribosomal subunit protein uL5 / 40S ribosomal protein S21-like protein / 60S ribosomal protein l23-like protein / Small ribosomal subunit protein eS24 類似検索 - 構成要素
ジャーナル: Nat Struct Mol Biol / 年: 2023 タイトル: Structural inventory of cotranslational protein folding by the eukaryotic RAC complex. 著者: Miglė Kišonaitė / Klemens Wild / Karine Lapouge / Genís Valentín Gesé / Nikola Kellner / Ed Hurt / Irmgard Sinning / 要旨: The challenge of nascent chain folding at the ribosome is met by the conserved ribosome-associated complex (RAC), which forms a chaperone triad with the Hsp70 protein Ssb in fungi, and consists of ...The challenge of nascent chain folding at the ribosome is met by the conserved ribosome-associated complex (RAC), which forms a chaperone triad with the Hsp70 protein Ssb in fungi, and consists of the non-canonical Hsp70 Ssz1 and the J domain protein Zuotin (Zuo1). Here we determine cryo-EM structures of Chaetomium thermophilum RAC bound to 80S ribosomes. RAC adopts two distinct conformations accommodating continuous ribosomal rotation by a flexible lever arm. It is held together by a tight interaction between the Ssz1 substrate-binding domain and the Zuo1 N terminus, and additional contacts between the Ssz1 nucleotide-binding domain and Zuo1 J- and Zuo1 homology domains, which form a rigid unit. The Zuo1 HPD motif conserved in J-proteins is masked in a non-canonical interaction by the Ssz1 nucleotide-binding domain, and allows the positioning of Ssb for activation by Zuo1. Overall, we provide the basis for understanding how RAC cooperates with Ssb in a dynamic nascent chain interaction and protein folding.