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Yorodumi- EMDB-14180: Human Oxytocin receptor (OTR) oxytocin Gq chimera (mGoqi) complex -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-14180 | |||||||||
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Title | Human Oxytocin receptor (OTR) oxytocin Gq chimera (mGoqi) complex | |||||||||
Map data | primary map | |||||||||
Sample |
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Function / homology | Function and homology information oxytocin receptor activity / response to anoxia / oxytocin receptor binding / neurohypophyseal hormone activity / V1A vasopressin receptor binding / guanyl nucleotide binding / Vasopressin-like receptors / regulation of systemic arterial blood pressure by vasopressin / vasopressin receptor activity / maternal process involved in parturition ...oxytocin receptor activity / response to anoxia / oxytocin receptor binding / neurohypophyseal hormone activity / V1A vasopressin receptor binding / guanyl nucleotide binding / Vasopressin-like receptors / regulation of systemic arterial blood pressure by vasopressin / vasopressin receptor activity / maternal process involved in parturition / positive regulation of penile erection / positive regulation of uterine smooth muscle contraction / positive regulation of norepinephrine secretion / sperm ejaculation / telencephalon development / mu-type opioid receptor binding / corticotropin-releasing hormone receptor 1 binding / maternal behavior / positive regulation of blood pressure / neuropeptide hormone activity / digestive tract development / positive regulation of synapse assembly / dopamine receptor signaling pathway / eating behavior / suckling behavior / social behavior / microvillus / peptide hormone binding / estrous cycle / G protein-coupled serotonin receptor binding / positive regulation of vasoconstriction / cellular response to hormone stimulus / ERK1 and ERK2 cascade / response to amphetamine / lactation / positive regulation of synaptic transmission, glutamatergic / response to cocaine / response to progesterone / muscle contraction / secretory granule / response to cytokine / female pregnancy / positive regulation of synaptic transmission, GABAergic / adherens junction / peptide binding / G-protein beta/gamma-subunit complex binding / Olfactory Signaling Pathway / Activation of the phototransduction cascade / G beta:gamma signalling through PLC beta / Presynaptic function of Kainate receptors / Thromboxane signalling through TP receptor / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / G-protein activation / G protein-coupled acetylcholine receptor signaling pathway / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / Prostacyclin signalling through prostacyclin receptor / Glucagon signaling in metabolic regulation / G beta:gamma signalling through CDC42 / ADP signalling through P2Y purinoceptor 12 / G beta:gamma signalling through BTK / Sensory perception of sweet, bitter, and umami (glutamate) taste / memory / response to peptide hormone / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / photoreceptor disc membrane / Adrenaline,noradrenaline inhibits insulin secretion / Glucagon-type ligand receptors / Vasopressin regulates renal water homeostasis via Aquaporins / G alpha (z) signalling events / cellular response to catecholamine stimulus / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / ADORA2B mediated anti-inflammatory cytokines production / adenylate cyclase-activating dopamine receptor signaling pathway / ADP signalling through P2Y purinoceptor 1 / G beta:gamma signalling through PI3Kgamma / cellular response to prostaglandin E stimulus / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / sensory perception of taste / GPER1 signaling / G-protein beta-subunit binding / heterotrimeric G-protein complex / Inactivation, recovery and regulation of the phototransduction cascade / extracellular vesicle / G alpha (12/13) signalling events / signaling receptor complex adaptor activity / Thrombin signalling through proteinase activated receptors (PARs) / response to estradiol / retina development in camera-type eye / GTPase binding / Ca2+ pathway / phospholipase C-activating G protein-coupled receptor signaling pathway / positive regulation of cold-induced thermogenesis / heart development / positive regulation of cytosolic calcium ion concentration / G alpha (i) signalling events / fibroblast proliferation / G alpha (s) signalling events / G alpha (q) signalling events / Ras protein signal transduction Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) / Mus musculus (house mouse) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.25 Å | |||||||||
Authors | Waltenspuhl Y / Ehrenmann J / Vacca S / Thom C / Medalia O / Pluckthun A | |||||||||
Funding support | Switzerland, 1 items
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Citation | Journal: Nat Commun / Year: 2022 Title: Structural basis for the activation and ligand recognition of the human oxytocin receptor. Authors: Yann Waltenspühl / Janosch Ehrenmann / Santiago Vacca / Cristian Thom / Ohad Medalia / Andreas Plückthun / Abstract: The small cyclic neuropeptide hormone oxytocin (OT) and its cognate receptor play a central role in the regulation of social behaviour and sexual reproduction. Here we report the single-particle cryo- ...The small cyclic neuropeptide hormone oxytocin (OT) and its cognate receptor play a central role in the regulation of social behaviour and sexual reproduction. Here we report the single-particle cryo-electron microscopy structure of the active oxytocin receptor (OTR) in complex with its cognate ligand oxytocin. Our structure provides high-resolution insights into the OT binding mode, the OTR activation mechanism as well as the subtype specificity within the oxytocin/vasopressin receptor family. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_14180.map.gz | 135.6 MB | EMDB map data format | |
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Header (meta data) | emd-14180-v30.xml emd-14180.xml | 20.1 KB 20.1 KB | Display Display | EMDB header |
Images | emd_14180.png | 52.9 KB | ||
Others | emd_14180_additional_1.map.gz | 129.4 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-14180 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-14180 | HTTPS FTP |
-Related structure data
Related structure data | 7qvmMC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_14180.map.gz / Format: CCP4 / Size: 266.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | primary map | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.651 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: raw map
File | emd_14180_additional_1.map | ||||||||||||
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Annotation | raw map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
+Entire : OTR in complex with Oxytocin, heterotrimeric mini-Gq chimera (Goq...
+Supramolecule #1: OTR in complex with Oxytocin, heterotrimeric mini-Gq chimera (Goq...
+Supramolecule #2: Heterotrimeric mini-Gq chimera (Goqi) and Oxytocin receptor
+Supramolecule #3: Oxytocin
+Supramolecule #4: Antibody fragment scFv16
+Macromolecule #1: Guanine nucleotide-binding protein G(o) subunit alpha,cDNA FLJ314...
+Macromolecule #2: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
+Macromolecule #3: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
+Macromolecule #4: Oxytocin
+Macromolecule #5: Oxytocin receptor
+Macromolecule #6: Antibody fragment scFv16
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: OTHER |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.8 µm |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 63.78 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
CTF correction | Software - Name: Gctf |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.25 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 392369 |