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- EMDB-14138: Bovine complex I in lipid nanodisc, Deactive-ligand (distal membr... -
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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Bovine complex I in lipid nanodisc, Deactive-ligand (distal membrane domain) | |||||||||
![]() | Globally sharpened focus-refined map generated using RELION Postprocess | |||||||||
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![]() | Mitochondrial complex I / Respiratory complex I / NADH:ubiquinone oxidoreductase / Nanodisc / ELECTRON TRANSPORT | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.31 Å | |||||||||
![]() | Chung I / Bridges HR / Hirst J | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM structures define ubiquinone-10 binding to mitochondrial complex I and conformational transitions accompanying Q-site occupancy Authors: Chung I / Wright JJ / Bridges HR / Ivanov BS / Biner O / Pereira CS / Arantes GM / Hirst J | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 920.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 16.5 KB 16.5 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 22.5 KB | Display | ![]() |
Images | ![]() | 48.3 KB | ||
Masks | ![]() | 1000 MB | ![]() | |
Filedesc metadata | ![]() | 4.7 KB | ||
Others | ![]() ![]() | 810.6 MB 810.1 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 854.5 KB | Display | ![]() |
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Full document | ![]() | 854.1 KB | Display | |
Data in XML | ![]() | 30.1 KB | Display | |
Data in CIF | ![]() | 40.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Globally sharpened focus-refined map generated using RELION Postprocess | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.7496 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: Half map 1 generated using RELION Postprocess (unsharpened...
File | emd_14138_half_map_1.map | ||||||||||||
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Annotation | Half map 1 generated using RELION Postprocess (unsharpened and unfiltered) | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map 2 generated using RELION Postprocess (unsharpened...
File | emd_14138_half_map_2.map | ||||||||||||
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Annotation | Half map 2 generated using RELION Postprocess (unsharpened and unfiltered) | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Focus-refinement of the distal membrane domain of mitochondrial c...
Entire | Name: Focus-refinement of the distal membrane domain of mitochondrial complex I in the deactive state with a ligand bound |
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Components |
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-Supramolecule #1: Focus-refinement of the distal membrane domain of mitochondrial c...
Supramolecule | Name: Focus-refinement of the distal membrane domain of mitochondrial complex I in the deactive state with a ligand bound type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 1 MDa |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 4.78 mg/mL | |||||||||
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Buffer | pH: 7.5 Component:
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Grid | Model: UltrAuFoil R0.6/1 / Material: GOLD / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 90 sec. Details: Following glow discharge for 90 s at 20 mA, the grid was treated for 48 hours in an anaerobic glovebox in ethanol containing 5 mM 11-mercaptoundecyl hexaethyleneglycol, washed three times in ...Details: Following glow discharge for 90 s at 20 mA, the grid was treated for 48 hours in an anaerobic glovebox in ethanol containing 5 mM 11-mercaptoundecyl hexaethyleneglycol, washed three times in ethanol and dried prior to blotting | |||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV / Details: blot for 10 seconds before plunging. |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Digitization - Dimensions - Width: 5760 pixel / Digitization - Dimensions - Height: 4092 pixel / Number grids imaged: 2 / Number real images: 6780 / Average exposure time: 2.4 sec. / Average electron dose: 40.5 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.4 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 81000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |