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- EMDB-13967: Cryo-EM structure of the human mtLSU assembly intermediate upon M... -

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Basic information

Entry
Database: EMDB / ID: EMD-13967
TitleCryo-EM structure of the human mtLSU assembly intermediate upon MRM2 depletion - class 4
Map data
Sample
  • Complex: Human mitochondrial ribosome large subunit
    • Protein or peptide: x 47 types
    • RNA: x 2 types
  • Ligand: x 3 types
KeywordsMitochondria / Ribosome / Assembly / Methyltransferase / MRM2 / RNA modification
Function / homology
Function and homology information


negative regulation of mitochondrial translation / mitochondrial large ribosomal subunit assembly / protein lipoylation / Complex I biogenesis / negative regulation of ribosome biogenesis / Mitochondrial Fatty Acid Beta-Oxidation / Protein lipoylation / positive regulation of mitochondrial translation / Respiratory electron transport / rRNA import into mitochondrion ...negative regulation of mitochondrial translation / mitochondrial large ribosomal subunit assembly / protein lipoylation / Complex I biogenesis / negative regulation of ribosome biogenesis / Mitochondrial Fatty Acid Beta-Oxidation / Protein lipoylation / positive regulation of mitochondrial translation / Respiratory electron transport / rRNA import into mitochondrion / mitochondrial translational elongation / Mitochondrial translation elongation / Mitochondrial translation termination / Mitochondrial translation initiation / mitochondrial [2Fe-2S] assembly complex / iron-sulfur cluster assembly complex / mitochondrial large ribosomal subunit / mitochondrial fission / mitochondrial large ribosomal subunit binding / mitochondrial ribosome / Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters / mitochondrial small ribosomal subunit / [2Fe-2S] cluster assembly / mitochondrial translation / iron-sulfur cluster assembly / acyl binding / acyl carrier activity / ribosomal large subunit binding / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / proton motive force-driven mitochondrial ATP synthesis / respiratory chain complex I / RNA processing / Mitochondrial protein degradation / cellular response to leukemia inhibitory factor / aerobic respiration / ribosomal large subunit biogenesis / fatty acid binding / mitochondrial membrane / fatty acid biosynthetic process / double-stranded RNA binding / 5S rRNA binding / small ribosomal subunit rRNA binding / endonuclease activity / mitochondrial inner membrane / negative regulation of translation / rRNA binding / nuclear body / ribosome / structural constituent of ribosome / mitochondrial matrix / protein domain specific binding / translation / ribonucleoprotein complex / nucleotide binding / mRNA binding / apoptotic process / calcium ion binding / mitochondrion / extracellular space / RNA binding / nucleoplasm / nucleus / plasma membrane / cytosol
Similarity search - Function
MIEF1-MP, LYR domain / Protein Iojap/ribosomal silencing factor RsfS / Ribosomal silencing factor during starvation / : / Ribosomal protein S30, mitochondrial / Ribosomal protein 63, mitochondrial / Growth arrest/ DNA-damage-inducible protein-interacting protein 1 / Ribosomal protein L35, mitochondrial / Ribosomal protein L51, mitochondrial / Growth arrest and DNA-damage-inducible proteins-interacting protein 1 ...MIEF1-MP, LYR domain / Protein Iojap/ribosomal silencing factor RsfS / Ribosomal silencing factor during starvation / : / Ribosomal protein S30, mitochondrial / Ribosomal protein 63, mitochondrial / Growth arrest/ DNA-damage-inducible protein-interacting protein 1 / Ribosomal protein L35, mitochondrial / Ribosomal protein L51, mitochondrial / Growth arrest and DNA-damage-inducible proteins-interacting protein 1 / Mitochondrial ribosomal subunit / Mitochondrial ribosome protein 63 / Ribosomal protein L37/S30 / Growth arrest and DNA damage-inducible proteins-interacting protein 1 domain superfamily / : / : / Mitochondrial 28S ribosomal protein S30 (PDCD9) / 39S ribosomal protein L52, mitochondrial / Mitoribosomal protein mL52 / MRPL44, double-stranded RNA binding domain / : / MRPL44 dsRNA-binding domain / Large ribosomal subunit protein mL44, endonuclease domain / : / Large ribosomal subunit protein bL9m C-terminal domain / Tim44-like domain / Tim44-like domain / Tim44 / : / : / Ribosomal protein L50, mitochondria / Ribosomal subunit 39S / Ribosomal protein L49/IMG2 / Mitochondrial large subunit ribosomal protein (Img2) / Ribosomal protein L27/L41, mitochondrial / : / Mitochondrial ribosomal protein L27 / 39S ribosomal protein L43/54S ribosomal protein L51 / Ribosomal protein L47, mitochondrial / MRP-L47 superfamily, mitochondrial / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily / TGS-like / TGS domain profile. / TGS / Ribonuclease III, endonuclease domain superfamily / Double stranded RNA-binding domain (dsRBD) profile. / Double-stranded RNA-binding domain / Complex 1 LYR protein domain / Complex 1 protein (LYR family) / : / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Beta-grasp domain superfamily / Acyl carrier protein (ACP) / NTF2-like domain superfamily / Nucleotidyltransferase superfamily / Phosphopantetheine attachment site / Phosphopantetheine attachment site. / Ribosomal protein L10-like domain superfamily / Ribosomal protein L10P / Ribosomal protein L10 / Phosphopantetheine attachment site / ACP-like superfamily / Ribosomal protein L28/L24 superfamily / Carrier protein (CP) domain profile. / Phosphopantetheine binding ACP domain / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal L28 family / Ribosomal protein L33 superfamily / Ribosomal protein L16 / Ribosomal protein L28/L24 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L30, bacterial-type / : / L28p-like / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L20 / Ribosomal L32p protein family / Ribosomal protein L19 / Ribosomal protein L19 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L19 superfamily / Ribosomal protein L21
Similarity search - Domain/homology
Mitochondrial ribosome and complex I assembly factor AltMIEF1 / Acyl carrier protein, mitochondrial / Large ribosomal subunit protein bL33m / Large ribosomal subunit protein uL3m / Large ribosomal subunit protein bL19m / Large ribosomal subunit protein bL28m / Large ribosomal subunit protein mL49 / Large ribosomal subunit protein mL51 / Large ribosomal subunit protein uL2m / Large ribosomal subunit protein uL14m ...Mitochondrial ribosome and complex I assembly factor AltMIEF1 / Acyl carrier protein, mitochondrial / Large ribosomal subunit protein bL33m / Large ribosomal subunit protein uL3m / Large ribosomal subunit protein bL19m / Large ribosomal subunit protein bL28m / Large ribosomal subunit protein mL49 / Large ribosomal subunit protein mL51 / Large ribosomal subunit protein uL2m / Large ribosomal subunit protein uL14m / Large ribosomal subunit protein bL21m / Large ribosomal subunit protein uL10m / Large ribosomal subunit protein mL52 / Large ribosomal subunit protein mL41 / Large ribosomal subunit protein mL50 / Large ribosomal subunit protein mL43 / Large ribosomal subunit protein mL64 / Large ribosomal subunit protein uL30m / Large ribosomal subunit protein uL24m / Mitochondrial assembly of ribosomal large subunit protein 1 / Large ribosomal subunit protein bL34m / Large ribosomal subunit protein mL63 / Large ribosomal subunit protein mL45 / Large ribosomal subunit protein bL32m / Large ribosomal subunit protein bL20m / Large ribosomal subunit protein uL13m / Large ribosomal subunit protein bL9m / Large ribosomal subunit protein uL4m / Large ribosomal subunit protein mL37 / Large ribosomal subunit protein uL18m / Large ribosomal subunit protein mL44 / Large ribosomal subunit protein uL29m / Large ribosomal subunit protein mL65 / Large ribosomal subunit protein bL17m / Large ribosomal subunit protein mL66 / Large ribosomal subunit protein uL22m / Large ribosomal subunit protein uL16m / Large ribosomal subunit protein mL39 / Large ribosomal subunit protein bL35m / Large ribosomal subunit protein uL15m / Large ribosomal subunit protein bL36m / Large ribosomal subunit protein bL27m
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.89 Å
AuthorsRebelo-Guiomar P / Pellegrino S / Dent KC / Warren AJ / Minczuk M
Funding support United Kingdom, 1 items
OrganizationGrant numberCountry
Medical Research Council (MRC, United Kingdom)MC_UU_00015/4 United Kingdom
CitationJournal: Nat Commun / Year: 2022
Title: A late-stage assembly checkpoint of the human mitochondrial ribosome large subunit.
Authors: Pedro Rebelo-Guiomar / Simone Pellegrino / Kyle C Dent / Aldema Sas-Chen / Leonor Miller-Fleming / Caterina Garone / Lindsey Van Haute / Jack F Rogan / Adam Dinan / Andrew E Firth / Byron ...Authors: Pedro Rebelo-Guiomar / Simone Pellegrino / Kyle C Dent / Aldema Sas-Chen / Leonor Miller-Fleming / Caterina Garone / Lindsey Van Haute / Jack F Rogan / Adam Dinan / Andrew E Firth / Byron Andrews / Alexander J Whitworth / Schraga Schwartz / Alan J Warren / Michal Minczuk /
Abstract: Many cellular processes, including ribosome biogenesis, are regulated through post-transcriptional RNA modifications. Here, a genome-wide analysis of the human mitochondrial transcriptome shows that ...Many cellular processes, including ribosome biogenesis, are regulated through post-transcriptional RNA modifications. Here, a genome-wide analysis of the human mitochondrial transcriptome shows that 2'-O-methylation is limited to residues of the mitoribosomal large subunit (mtLSU) 16S mt-rRNA, introduced by MRM1, MRM2 and MRM3, with the modifications installed by the latter two proteins being interdependent. MRM2 controls mitochondrial respiration by regulating mitoribosome biogenesis. In its absence, mtLSU particles (visualized by cryo-EM at the resolution of 2.6 Å) present disordered RNA domains, partial occupancy of bL36m and bound MALSU1:L0R8F8:mtACP anti-association module, allowing five mtLSU biogenesis intermediates with different intersubunit interface configurations to be placed along the assembly pathway. However, mitoribosome biogenesis does not depend on the methyltransferase activity of MRM2. Disruption of the MRM2 Drosophila melanogaster orthologue leads to mitochondria-related developmental arrest. This work identifies a key checkpoint during mtLSU assembly, essential to maintain mitochondrial homeostasis.
History
DepositionDec 10, 2021-
Header (metadata) releaseMay 18, 2022-
Map releaseMay 18, 2022-
UpdateNov 13, 2024-
Current statusNov 13, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_13967.png

Downloads & links

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Map

FileDownload / File: emd_13967.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.06 Å/pix.
x 360 pix.
= 381.6 Å		1.06 Å/pix.
x 360 pix.
= 381.6 Å		1.06 Å/pix.
x 360 pix.
= 381.6 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.06 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.01
Minimum - Maximum-0.0165175 - 0.06569443
Average (Standard dev.)-0.000100456324 (±0.003870605)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 381.59998 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Human mitochondrial ribosome large subunit

EntireName: Human mitochondrial ribosome large subunit
Components
  • Complex: Human mitochondrial ribosome large subunit
    • Protein or peptide: 39S ribosomal protein L2, mitochondrial
    • Protein or peptide: 39S ribosomal protein L3, mitochondrial
    • Protein or peptide: 39S ribosomal protein L4, mitochondrial
    • Protein or peptide: 39S ribosomal protein L9, mitochondrial
    • Protein or peptide: 39S ribosomal protein L10, mitochondrial
    • Protein or peptide: 39S ribosomal protein L13, mitochondrial
    • Protein or peptide: 39S ribosomal protein L14, mitochondrial
    • Protein or peptide: 39S ribosomal protein L15, mitochondrial
    • Protein or peptide: 39S ribosomal protein L16, mitochondrial
    • Protein or peptide: 39S ribosomal protein L17, mitochondrial
    • Protein or peptide: 39S ribosomal protein L18, mitochondrial
    • Protein or peptide: 39S ribosomal protein L19, mitochondrial
    • Protein or peptide: 39S ribosomal protein L20, mitochondrial
    • Protein or peptide: 39S ribosomal protein L21, mitochondrial
    • Protein or peptide: 39S ribosomal protein L22, mitochondrial
    • Protein or peptide: 39S ribosomal protein L23, mitochondrial
    • Protein or peptide: 39S ribosomal protein L24, mitochondrial
    • Protein or peptide: 39S ribosomal protein L27, mitochondrial
    • Protein or peptide: 39S ribosomal protein L28, mitochondrial
    • Protein or peptide: 39S ribosomal protein L47, mitochondrial
    • Protein or peptide: 39S ribosomal protein L30, mitochondrial
    • Protein or peptide: 39S ribosomal protein L32, mitochondrial
    • Protein or peptide: 39S ribosomal protein L33, mitochondrial
    • Protein or peptide: 39S ribosomal protein L34, mitochondrial
    • Protein or peptide: 39S ribosomal protein L35, mitochondrial
    • Protein or peptide: 39S ribosomal protein L37, mitochondrial
    • Protein or peptide: 39S ribosomal protein L38, mitochondrial
    • Protein or peptide: 39S ribosomal protein L39, mitochondrial
    • Protein or peptide: 39S ribosomal protein L41, mitochondrial
    • Protein or peptide: 39S ribosomal protein L42, mitochondrial
    • Protein or peptide: 39S ribosomal protein L43, mitochondrial
    • Protein or peptide: 39S ribosomal protein L44, mitochondrial
    • Protein or peptide: 39S ribosomal protein L45, mitochondrial
    • Protein or peptide: 39S ribosomal protein L48, mitochondrial
    • Protein or peptide: 39S ribosomal protein L49, mitochondrial
    • Protein or peptide: 39S ribosomal protein L50, mitochondrial
    • Protein or peptide: 39S ribosomal protein L51, mitochondrial
    • Protein or peptide: 39S ribosomal protein L52, mitochondrial
    • Protein or peptide: Ribosomal protein 63, mitochondrial
    • Protein or peptide: Peptidyl-tRNA hydrolase ICT1, mitochondrial
    • Protein or peptide: Growth arrest and DNA damage-inducible proteins-interacting protein 1
    • Protein or peptide: 39S ribosomal protein S18a, mitochondrial
    • Protein or peptide: 39S ribosomal protein S30, mitochondrial
    • Protein or peptide: Mitochondrial assembly of ribosomal large subunit protein 1
    • Protein or peptide: MIEF1 upstream open reading frame protein
    • Protein or peptide: Acyl carrier protein, mitochondrial
    • RNA: 16S ribosomal RNA
    • RNA: mitochondrial tRNAVal
    • Protein or peptide: 39S ribosomal protein L36, mitochondrial
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: water

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Supramolecule #1: Human mitochondrial ribosome large subunit

SupramoleculeName: Human mitochondrial ribosome large subunit / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#49
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: 39S ribosomal protein L2, mitochondrial

MacromoleculeName: 39S ribosomal protein L2, mitochondrial / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 23.83448 KDa
SequenceString: ANFVSWKSRT KYTITPVKMR KSGGRDHTGR IRVHGIGGGH KQRYRMIDFL RFRPEETKSG PFEEKVIQVR YDPCRSADIA LVAGGSRKR WIIATENMQA GDTILNSNHI GRMAVAAREG DAHPLGALPV GTLINNVESE PGRGAQYIRA AGTCGVLLRK V NGTAIIQL ...String:
ANFVSWKSRT KYTITPVKMR KSGGRDHTGR IRVHGIGGGH KQRYRMIDFL RFRPEETKSG PFEEKVIQVR YDPCRSADIA LVAGGSRKR WIIATENMQA GDTILNSNHI GRMAVAAREG DAHPLGALPV GTLINNVESE PGRGAQYIRA AGTCGVLLRK V NGTAIIQL PSKRQMQVLE TCVATVGRVS NVDHNKRVIG KAGRNRWLGK RPNSGRW

UniProtKB: Large ribosomal subunit protein uL2m

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Macromolecule #2: 39S ribosomal protein L3, mitochondrial

MacromoleculeName: 39S ribosomal protein L3, mitochondrial / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 34.023105 KDa
SequenceString: SGTWWDEHLS EENVPFIKQL VSDEDKAQLA SKLCPLKDEP WPIHPWEPGS FRVGLIALKL GMMPLWTKDG QKHVVTLLQV QDCHVLKYT SKENCNGKMA TLSVGGKTVS RFRKATSILE FYRELGLPPK QTVKIFNITD NAAIKPGTPL YAAHFRPGQY V DVTAKTIG ...String:
SGTWWDEHLS EENVPFIKQL VSDEDKAQLA SKLCPLKDEP WPIHPWEPGS FRVGLIALKL GMMPLWTKDG QKHVVTLLQV QDCHVLKYT SKENCNGKMA TLSVGGKTVS RFRKATSILE FYRELGLPPK QTVKIFNITD NAAIKPGTPL YAAHFRPGQY V DVTAKTIG KGFQGVMKRW GFKGQPATHG QTKTHRRPGA VATGDIGRVW PGTKMPGKMG NIYRTEYGLK VWRINTKHNI IY VNGSVPG HKNCLVKVKD SKLPAYKDLG KNLPFPTYFP DGDEEELPED LYDENVCQPG APSITFA

UniProtKB: Large ribosomal subunit protein uL3m

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Macromolecule #3: 39S ribosomal protein L4, mitochondrial

MacromoleculeName: 39S ribosomal protein L4, mitochondrial / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 28.528051 KDa
SequenceString: EPVLRKVELP VPTHRRPVQA WVESLRGFEQ ERVGLADLHP DVFATAPRLD ILHQVAMWQK NFKRISYAKT KTRAEVRGGG RKPWPQKGT GRARHGSIRS PLWRGGGVAH GPRGPTSYYY MLPMKVRALG LKVALTVKLA QDDLHIMDSL ELPTGDPQYL T ELAHYRRW ...String:
EPVLRKVELP VPTHRRPVQA WVESLRGFEQ ERVGLADLHP DVFATAPRLD ILHQVAMWQK NFKRISYAKT KTRAEVRGGG RKPWPQKGT GRARHGSIRS PLWRGGGVAH GPRGPTSYYY MLPMKVRALG LKVALTVKLA QDDLHIMDSL ELPTGDPQYL T ELAHYRRW GDSVLLVDLT HEEMPQSIVE ATSRLKTFNL IPAVGLNVHS MLKHQTLVLT LPTVAFLEDK LLWQDSRYRP LY PFSLPYS DFP

UniProtKB: Large ribosomal subunit protein uL4m

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Macromolecule #4: 39S ribosomal protein L9, mitochondrial

MacromoleculeName: 39S ribosomal protein L9, mitochondrial / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 11.127014 KDa
SequenceString:
TVIVERWWKV PLAGEGRKPR LHRRHRVYKL VEDTKHRPKE NLELILTQSV ENVGVRGDLV SVKKSLGRNR LLPQGLAVYA SPENKKLFE EEKLLR

UniProtKB: Large ribosomal subunit protein bL9m

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Macromolecule #5: 39S ribosomal protein L10, mitochondrial

MacromoleculeName: 39S ribosomal protein L10, mitochondrial / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 3.552307 KDa
SequenceString:
RRVMHFQRQK LMAVTEYIPP KPAIHPSCLP

UniProtKB: Large ribosomal subunit protein uL10m

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Macromolecule #6: 39S ribosomal protein L13, mitochondrial

MacromoleculeName: 39S ribosomal protein L13, mitochondrial / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 20.591789 KDa
SequenceString:
SSFSRAPQQW ATFARIWYLL DGKMQPPGKL AAMASIRLQG LHKPVYHALS DCGDHVVIMN TRHIAFSGNK WEQKVYSSHT GYPGGFRQV TAAQLHLRDP VAIVKLAIYG MLPKNLHRRT MMERLHLFPD EYIPEDILKN LVEELPQPRK IPKRLDEYTQ E EIDAFPRL WTPPEDYRL

UniProtKB: Large ribosomal subunit protein uL13m

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Macromolecule #7: 39S ribosomal protein L14, mitochondrial

MacromoleculeName: 39S ribosomal protein L14, mitochondrial / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 12.710934 KDa
SequenceString:
AIQKMTRVRV VDNSALGNSP YHRAPRCIHV YKKNGVGKVG DQILLAIKGQ KKKALIVGHC MPGPRMTPRF DSNNVVLIED NGNPVGTRI KTPIPTSLRK REGEYSKVLA IAQNFV

UniProtKB: Large ribosomal subunit protein uL14m

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Macromolecule #8: 39S ribosomal protein L15, mitochondrial

MacromoleculeName: 39S ribosomal protein L15, mitochondrial / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 32.704938 KDa
SequenceString: ARALDLLRGL PRVSLANLKP NPGSKKPERR PRGRRRGRKC GRGHKGERQR GTRPRLGFEG GQTPFYIRIP KYGFNEGHSF RRQYKPLSL NRLQYLIDLG RVDPSQPIDL TQLVNGRGVT IQPLKRDYGV QLVEEGADTF TAKVNIEVQL ASELAIAAIE K NGGVVTTA ...String:
ARALDLLRGL PRVSLANLKP NPGSKKPERR PRGRRRGRKC GRGHKGERQR GTRPRLGFEG GQTPFYIRIP KYGFNEGHSF RRQYKPLSL NRLQYLIDLG RVDPSQPIDL TQLVNGRGVT IQPLKRDYGV QLVEEGADTF TAKVNIEVQL ASELAIAAIE K NGGVVTTA FYDPRSLDIV CKPVPFFLRG QPIPKRMLPP EELVPYYTDA KNRGYLADPA KFPEARLELA RKYGYILPDI TK DELFKML CTRKDPRQIF FGLAPGWVVN MADKKILKPT DENLLKYYTS

UniProtKB: Large ribosomal subunit protein uL15m

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Macromolecule #9: 39S ribosomal protein L16, mitochondrial

MacromoleculeName: 39S ribosomal protein L16, mitochondrial / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 23.06982 KDa
SequenceString: RFIERAPLVP KVRREPKNLS DIRGPSTEAT EFTEGNFAIL ALGGGYLHWG HFEMMRLTIN RSMDPKNMFA IWRVPAPFKP ITRKSVGHR MGGGKGAIDH YVTPVKAGRL VVEMGGRCEF EEVQGFLDQV AHKLPFAAKA VSRGTLEKMR KDQEERERNN Q NPWTFERI ...String:
RFIERAPLVP KVRREPKNLS DIRGPSTEAT EFTEGNFAIL ALGGGYLHWG HFEMMRLTIN RSMDPKNMFA IWRVPAPFKP ITRKSVGHR MGGGKGAIDH YVTPVKAGRL VVEMGGRCEF EEVQGFLDQV AHKLPFAAKA VSRGTLEKMR KDQEERERNN Q NPWTFERI ATANMLGIRK VLSPYDLTHK GKYWGKFYMP KRV

UniProtKB: Large ribosomal subunit protein uL16m

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Macromolecule #10: 39S ribosomal protein L17, mitochondrial

MacromoleculeName: 39S ribosomal protein L17, mitochondrial / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 17.761713 KDa
SequenceString:
ISHGRVFRRM GLGPESRIHL LRNLLTGLVR HERIEAPWAR VDEMRGYAEK LIDYGKLGDT NERAMRMADF WLTEKDLIPK LFQVLAPRY KDQTGGYTRM LQIPNRSLDR AKMAVIEYKG NCLPPLPLPR RDSHLTLLNQ LLQGLRQDLR QSQ

UniProtKB: Large ribosomal subunit protein bL17m

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Macromolecule #11: 39S ribosomal protein L18, mitochondrial

MacromoleculeName: 39S ribosomal protein L18, mitochondrial / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 16.335679 KDa
SequenceString:
VENEAVAPEF TNRNPRNLEL LSVARKERGW RTVFPSREFW HRLRVIRTQH HVEALVEHQN GKVVVSASTR EWAIKKHLYS TRNVVACES IGRVLAQRCL EAGINFMVYQ PTPWEAASDS MKRLQSAMTE GGVVLREPQR IY

UniProtKB: Large ribosomal subunit protein uL18m

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Macromolecule #12: 39S ribosomal protein L19, mitochondrial

MacromoleculeName: 39S ribosomal protein L19, mitochondrial / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 25.674828 KDa
SequenceString: RFLSPEFIPR RGRTDPLKFQ IERKDMLERR KVLHIPEFYV GSILRVTTAD PYASGKISQF LGICIQRSGR GLGATFILRN VIEGQGVEI CFELYNPRVQ EIQVVKLEKR LDDSLLYLRD ALPEYSTFDV NMKPVVQEPN QKVPVNELKV KMKPKPWSKR W ERPNFNIK ...String:
RFLSPEFIPR RGRTDPLKFQ IERKDMLERR KVLHIPEFYV GSILRVTTAD PYASGKISQF LGICIQRSGR GLGATFILRN VIEGQGVEI CFELYNPRVQ EIQVVKLEKR LDDSLLYLRD ALPEYSTFDV NMKPVVQEPN QKVPVNELKV KMKPKPWSKR W ERPNFNIK GIRFDLCLTE QQMKEAQKWN QPWLEFDMMR EYDTSKIEAA IWKEIEASK

UniProtKB: Large ribosomal subunit protein bL19m

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Macromolecule #13: 39S ribosomal protein L20, mitochondrial

MacromoleculeName: 39S ribosomal protein L20, mitochondrial / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 16.251168 KDa
SequenceString:
LRNRVTDRYF RIQEVLKHAR HFRGRKNRCY RLAVRTVIRA FVKCTKARYL KKKNMRTLWI NRITAASQEH GLKYPALIGN LVKCQVELN RKVLADLAIY EPKTFKSLAA LASRRRHEGF AAALGDGKEP EGIFSRVVQY

UniProtKB: Large ribosomal subunit protein bL20m

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Macromolecule #14: 39S ribosomal protein L21, mitochondrial

MacromoleculeName: 39S ribosomal protein L21, mitochondrial / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 17.794934 KDa
SequenceString:
GYVPKTSLSS PPWPEVVLPD PVEETRHHAE VVKKVNEMIV TGQYGRLFAV VHFASRQWKV TSEDLILIGN ELDLACGERI RLEKVLLVG ADNFTLLGKP LLGKDLVRVE ATVIEKTESW PRIIMRFRKR KNFKKKRIVT TPQTVLRINS IEIAPCL

UniProtKB: Large ribosomal subunit protein bL21m

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Macromolecule #15: 39S ribosomal protein L22, mitochondrial

MacromoleculeName: 39S ribosomal protein L22, mitochondrial / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 19.466799 KDa
SequenceString:
ISRKWEKKNK IVYPPQLPGE PRRPAEIYHC RRQIKYSKDK MWYLAKLIRG MSIDQALAQL EFNDKKGAKI IKEVLLEAQD MAVRDHNVE FRSNLYIAES TSGRGQCLKR IRYHGRGRFG IMEKVYCHYF VKLVEGPPPP PEPPKTAVAH AKEYIQQLRS R TIVHTL

UniProtKB: Large ribosomal subunit protein uL22m

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Macromolecule #16: 39S ribosomal protein L23, mitochondrial

MacromoleculeName: 39S ribosomal protein L23, mitochondrial / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 14.562766 KDa
SequenceString:
ARNVVYPLYR LGGPQLRVFR TNFFIQLVRP GVAQPEDTVQ FRIPMEMTRV DLRNYLEGIY NVPVAAVRTR VQHGSNKRRD HRNVRIKKP DYKVAYVQLA HGQTFTFPDL FPSDPRRGGV PSWFGL

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Macromolecule #17: 39S ribosomal protein L24, mitochondrial

MacromoleculeName: 39S ribosomal protein L24, mitochondrial / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 5.751602 KDa
SequenceString:
TWIDGPKDTS VEDALERTYV PCLKTLQEEV MEAMGIKETR KYKKVYWY

UniProtKB: Large ribosomal subunit protein uL24m

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Macromolecule #18: 39S ribosomal protein L27, mitochondrial

MacromoleculeName: 39S ribosomal protein L27, mitochondrial / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 11.364354 KDa
SequenceString:
RRQGIKKMEG HYVHAGNIIA TQRHFRWHPG AHVGVGKNKC LYALEEGIVR YTKEVYVPHP RNTEAVDLIT RLPKGAVLYK TFVHVVPAK PEGTFKLVAM L

UniProtKB: Large ribosomal subunit protein bL27m

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Macromolecule #19: 39S ribosomal protein L28, mitochondrial

MacromoleculeName: 39S ribosomal protein L28, mitochondrial / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 28.81826 KDa
SequenceString: PLHKYPVWLW KRLQLREGIC SRLPGHYLRS LEEERTPTPV HYRPHGAKFK INPKNGQRER VEDVPIPIYF PPESQRGLWG GEGWILGQI YANNDKLSKR LKKVWKPQLF EREFYSEILD KKFTVTVTMR TLDLIDEAYG LDFYILKTPK EDLCSKFGMD L KRGMLLRL ...String:
PLHKYPVWLW KRLQLREGIC SRLPGHYLRS LEEERTPTPV HYRPHGAKFK INPKNGQRER VEDVPIPIYF PPESQRGLWG GEGWILGQI YANNDKLSKR LKKVWKPQLF EREFYSEILD KKFTVTVTMR TLDLIDEAYG LDFYILKTPK EDLCSKFGMD L KRGMLLRL ARQDPQLHPE DPERRAAIYD KYKEFAIPEE EAEWVGLTLE EAIEKQRLLE EKDPVPLFKI YVAELIQQLQ QQ ALS

UniProtKB: Large ribosomal subunit protein bL28m

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Macromolecule #20: 39S ribosomal protein L47, mitochondrial

MacromoleculeName: 39S ribosomal protein L47, mitochondrial / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 21.360918 KDa
SequenceString:
GLEEFFDDPK NWGQEKVKSG AAWTCQQLRN KSNEDLHKLW YVLLKERNML LTLEQEAKRQ RLPMPSPERL DKVVDSMDAL DKVVQERED ALRLLQTGQE RARPGAWRRD IFGRIIWHKF KQWVIPWHLN KRYNRKRFFA LPYVDHFLRL EREKRARIKA R KENLERKK AKILLKK

UniProtKB: Large ribosomal subunit protein uL29m

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Macromolecule #21: 39S ribosomal protein L30, mitochondrial

MacromoleculeName: 39S ribosomal protein L30, mitochondrial / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 13.332625 KDa
SequenceString:
KFTRSRIPEK VFQASPEDHE KYGGDPQNPH KLHIVTRIKS TRRRPYWEKD IIKMLGLEKA HTPQVHKNIP SVNAKLKVVK HLIRIKPLK LPQGLPAEEN MSNTCLKSTG ELVVQW

UniProtKB: Large ribosomal subunit protein uL30m

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Macromolecule #22: 39S ribosomal protein L32, mitochondrial

MacromoleculeName: 39S ribosomal protein L32, mitochondrial / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 12.606611 KDa
SequenceString:
AAPKNRRTIE VNRCRRRNPQ KLIKVKNNID VCPECGHLKQ KHVLCAYCYE KVCKETAEIR RQIGKQEGGP FKAPTIETVV LYTGETPSE QDQGKRIIER DRKRPSWFT

UniProtKB: Large ribosomal subunit protein bL32m

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Macromolecule #23: 39S ribosomal protein L33, mitochondrial

MacromoleculeName: 39S ribosomal protein L33, mitochondrial / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 5.831021 KDa
SequenceString:
KNILVRMVSE AGTGFCFNTK RNRLREKLTL LHYDPVVKQR VLFVEKKKI

UniProtKB: Large ribosomal subunit protein bL33m

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Macromolecule #24: 39S ribosomal protein L34, mitochondrial

MacromoleculeName: 39S ribosomal protein L34, mitochondrial / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 5.239168 KDa
SequenceString:
ARGNEYQPSN IKRKNKHGWV RRLSTPAGVQ VILRRMLKGR KSLSH

UniProtKB: Large ribosomal subunit protein bL34m

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Macromolecule #25: 39S ribosomal protein L35, mitochondrial

MacromoleculeName: 39S ribosomal protein L35, mitochondrial / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 11.788066 KDa
SequenceString:
LTYFSARKGK RKTVKAVIDR FLRLHCGLWV RRKAGYKKKL WKKTPARKKR LREFVFCNKT QSKLLDKMTT SFWKRRNWYV DDPYQKYHD RTNLKV

UniProtKB: Large ribosomal subunit protein bL35m

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Macromolecule #26: 39S ribosomal protein L37, mitochondrial

MacromoleculeName: 39S ribosomal protein L37, mitochondrial / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 45.302047 KDa
SequenceString: YEWGVRSTRK SEPPPLDRVY EIPGLEPITF AGKMHFVPWL ARPIFPPWDR GYKDPRFYRS PPLHEHPLYK DQACYIFHHR CRLLEGVKQ ALWLTKTKLI EGLPEKVLSL VDDPRNHIEN QDECVLNVIS HARLWQTTEE IPKRETYCPV IVDNLIQLCK S QILKHPSL ...String:
YEWGVRSTRK SEPPPLDRVY EIPGLEPITF AGKMHFVPWL ARPIFPPWDR GYKDPRFYRS PPLHEHPLYK DQACYIFHHR CRLLEGVKQ ALWLTKTKLI EGLPEKVLSL VDDPRNHIEN QDECVLNVIS HARLWQTTEE IPKRETYCPV IVDNLIQLCK S QILKHPSL ARRICVQNST FSATWNRESL LLQVRGSGGA RLSTKDPLPT IASREEIEAT KNHVLETFYP ISPIIDLHEC NI YDVKNDT GFQEGYPYPY PHTLYLLDKA NLRPHRLQPD QLRAKMILFA FGSALAQARL LYGNDAKVLE QPVVVQSVGT DGR VFHFLV FQLNTTDLDC NEGVKNLAWV DSDQLLYQHF WCLPVIKKRV VVEPVGPVGF KPETFRKFLA LYLHGA

UniProtKB: Large ribosomal subunit protein mL37

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Macromolecule #27: 39S ribosomal protein L38, mitochondrial

MacromoleculeName: 39S ribosomal protein L38, mitochondrial / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 34.730168 KDa
SequenceString: RRTPPLGPMP NSDIDLSNLE RLEKYRSFDR YRRRAEQEAQ APHWWRTYRE YFGPLDAVRA EWERTCGPYH KQRLAEYYGL YRDLFHGAT FVPRVPLHVA YAVGEDDLMP VYCGNEVTPT EAAQAPEVTY EAELWTLLLT SLDGHLLEPD AEYLHWLLTN I PGNRVAEG ...String:
RRTPPLGPMP NSDIDLSNLE RLEKYRSFDR YRRRAEQEAQ APHWWRTYRE YFGPLDAVRA EWERTCGPYH KQRLAEYYGL YRDLFHGAT FVPRVPLHVA YAVGEDDLMP VYCGNEVTPT EAAQAPEVTY EAELWTLLLT SLDGHLLEPD AEYLHWLLTN I PGNRVAEG QVTCPYLPPF PARGSGIHRL AFLLFKQDQP IDFSYQLAQR TFRTFDFYKK HQETMTPAGL SFFQCRWDDS VT YIFHQLL DMREPVFEFV RPPPYHPKQK RFPHRQPLRY LDRYRDSHEP TYGIY

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Macromolecule #28: 39S ribosomal protein L39, mitochondrial

MacromoleculeName: 39S ribosomal protein L39, mitochondrial / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 33.289535 KDa
SequenceString: SPTELTEMRN DLFNKEKARQ LSLTPRTEKI EVKHVGKTDP GTVFVMNKNI STPYSCAMHL SEWYCRKSIL ALVDGQPWDM YKPLTKSCE IKFLTFKDCD PGEVNKAYWR SCAMMMGCVI ERAFKDEYMV NLVRAPEVPV ISGAFCYDVV LDSKLDEWMP T KENLRSFT ...String:
SPTELTEMRN DLFNKEKARQ LSLTPRTEKI EVKHVGKTDP GTVFVMNKNI STPYSCAMHL SEWYCRKSIL ALVDGQPWDM YKPLTKSCE IKFLTFKDCD PGEVNKAYWR SCAMMMGCVI ERAFKDEYMV NLVRAPEVPV ISGAFCYDVV LDSKLDEWMP T KENLRSFT KDAHALIYKD LPFETLEVEA KVALEIFQHS KYKVDFIEEK ASQNPERIVK LHRIGDFIDV SEGPLIPRTS IC FQYEVSA VHNLQPTQPS LIRRFQGVSL PVHLRAHFTI WDKLLERSRK

UniProtKB: Large ribosomal subunit protein mL39

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Macromolecule #29: 39S ribosomal protein L41, mitochondrial

MacromoleculeName: 39S ribosomal protein L41, mitochondrial / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 14.032986 KDa
SequenceString:
ADRMSKWTSK RGPRSFRGRK GRGAKGIGFL TSGWRFVQIK EMVPEFVVPD LTGFKLKPYV SYLAPESEET PLTAAQLFSE AVAPAIEKD FKDGTFDPDN LEKYGFEPTQ EGKLFQLYPR NFLR

UniProtKB: Large ribosomal subunit protein mL41

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Macromolecule #30: 39S ribosomal protein L42, mitochondrial

MacromoleculeName: 39S ribosomal protein L42, mitochondrial / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 9.578935 KDa
SequenceString:
LPDDYNCNVE LALTSDGRTI VCYHPSVDIP YEHTKPIPRH LEEGPMIEQL SKMFFTTKHR WYPHGRYHRC RKNLNPPKDR

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Macromolecule #31: 39S ribosomal protein L43, mitochondrial

MacromoleculeName: 39S ribosomal protein L43, mitochondrial / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 16.741986 KDa
SequenceString:
TARGTPSRFL ASVLHNGLGR YVQQLQRLSF SVSRDGASSR GAREFVEREV IDFARRNPGV VIYVNSRPCC VPRVVAEYLN GAVREESIH CKSVEEISTL VQKLADQSGL DVIRIRKPFH TDNPSIQGQW HPFTNKPTTF RGLRPREVQ

UniProtKB: Large ribosomal subunit protein mL43

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Macromolecule #32: 39S ribosomal protein L44, mitochondrial

MacromoleculeName: 39S ribosomal protein L44, mitochondrial / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
EC number: Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 32.77352 KDa
SequenceString: VKKGFRAAFR FQKELERQRL LRCPPPPVRR SEKPNWDYHA EIQAFGHRLQ ENFSLDLLKT AFVNSCYIKS EEAKRQQLGI EKEAVLLNL KSNQELSEQG TSFSQTCLTQ FLEDEYPDMP TEGIKNLVDF LTGEEVVCHV ARNLAVEQLT LSEEFPVPPA V LQQTFFAV ...String:
VKKGFRAAFR FQKELERQRL LRCPPPPVRR SEKPNWDYHA EIQAFGHRLQ ENFSLDLLKT AFVNSCYIKS EEAKRQQLGI EKEAVLLNL KSNQELSEQG TSFSQTCLTQ FLEDEYPDMP TEGIKNLVDF LTGEEVVCHV ARNLAVEQLT LSEEFPVPPA V LQQTFFAV IGALLQSSGP ERTALFIRDF LITQMTGKEL FEMWKIINPM GLLVEELKKR NVSAPESRLT RQSGGTTALP LY FVGLYCD KKLIAEGPGE TVLVAEEEAA RVALRKLYGF TENRRPWNYS

UniProtKB: Large ribosomal subunit protein mL44

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Macromolecule #33: 39S ribosomal protein L45, mitochondrial

MacromoleculeName: 39S ribosomal protein L45, mitochondrial / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 19.852926 KDa
SequenceString:
ASQVSIRRIK DYDANFKIKD FPEKAKDIFI EAHLCLNNSD HDRLHTLVTE HCFPDMTWDI KYKTVRWSFV ESLEPSHVVQ VRCSSMMNQ GNVYGQITVR MHTRQTLAIY DRFGRLMYGQ EDVPKDVLEY VVFEKQLTNP YGSWRMHTKI VPPWAPPKQP I LKTVMIPG P

UniProtKB: Large ribosomal subunit protein mL45

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Macromolecule #34: 39S ribosomal protein L48, mitochondrial

MacromoleculeName: 39S ribosomal protein L48, mitochondrial / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 2.019455 KDa
SequenceString:
YKTKPTHGIG KYKHLIK

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Macromolecule #35: 39S ribosomal protein L49, mitochondrial

MacromoleculeName: 39S ribosomal protein L49, mitochondrial / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.087213 KDa
SequenceString:
FVESVDEYQF VERLLPATRI PDPPKHEHYP TPSGWQPPRD PPPNLPYFVR RSRMHNIPVY KDITHGNRQM TVIRKVEGDI WALQKDVED FLSPLLGKTP VTQVNEVTGT LRIKGYFDQE LKAWLLEKGF

UniProtKB: Large ribosomal subunit protein mL49

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Macromolecule #36: 39S ribosomal protein L50, mitochondrial

MacromoleculeName: 39S ribosomal protein L50, mitochondrial / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 12.10761 KDa
SequenceString:
PPLRSRAYTP PEDLQSRLES YVKEVFGSSL PSNWQDISLE DSRLKFNLLA HLADDLGHVV PNSRLHQMCR VRDVLDFYNV PIQDRSKFD ELSASNLPPN LKITWS

UniProtKB: Large ribosomal subunit protein mL50

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Macromolecule #37: 39S ribosomal protein L51, mitochondrial

MacromoleculeName: 39S ribosomal protein L51, mitochondrial / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 11.73991 KDa
SequenceString:
IGIRLTLPPP KVVDRWNEKR AMFGVYDNIG ILGNFEKHPK ELIRGPIWLR GWKGNELQRC IRKRKMVGSR MFADDLHNLN KRIRYLYKH FNRHGKFR

UniProtKB: Large ribosomal subunit protein mL51

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Macromolecule #38: 39S ribosomal protein L52, mitochondrial

MacromoleculeName: 39S ribosomal protein L52, mitochondrial / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 9.801988 KDa
SequenceString:
AGGQWRLQQG LAANPSGYGP LTELPDWSYA DGRPAPPMKG QLRRKAERET FARRVVLLSQ EMDAGLQAWQ LRQQKLQEEQ RKQENA

UniProtKB: Large ribosomal subunit protein mL52

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Macromolecule #39: Ribosomal protein 63, mitochondrial

MacromoleculeName: Ribosomal protein 63, mitochondrial / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 9.768266 KDa
SequenceString:
RRPRFVSLRA KQNMIRRLEI EAENHYWLSM PYMTREQERG HAAVRRREAF EAIKAAATSK FPPHRFIADQ LDHLNVTKKW S

UniProtKB: Large ribosomal subunit protein mL63

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Macromolecule #40: Peptidyl-tRNA hydrolase ICT1, mitochondrial

MacromoleculeName: Peptidyl-tRNA hydrolase ICT1, mitochondrial / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 14.901154 KDa
SequenceString:
EFKSIYSLDK LYPESQGSDT AWRVDIPLDR LTISYCRNSK AEVRFHLATA EWIAEPVRQK IAITHKNKIN RLGELILTSE SSRYQFRNL ADCLQKIRDM ITEASQKLHR IRIENMNRER LRQKRI

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Macromolecule #41: Growth arrest and DNA damage-inducible proteins-interacting protein 1

MacromoleculeName: Growth arrest and DNA damage-inducible proteins-interacting protein 1
type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 11.942498 KDa
SequenceString:
YRARPPPRRR PGPRWPDPED LLTPRWQLGP RYAAKQFARY GAASGVVPGS LWPSPEQLRE LEAEEREWYP SLATMQESLR VKQLAEEQK RREREQHIAE CM

UniProtKB: Large ribosomal subunit protein mL64

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Macromolecule #42: 39S ribosomal protein S18a, mitochondrial

MacromoleculeName: 39S ribosomal protein S18a, mitochondrial / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 16.351198 KDa
SequenceString:
PKESPNPPNP SGQCPICRWN LKHKYNYDDV LLLSQFIRPH GGMLPRKITG LCQEEHRKIE ECVKMAHRAG LLPNHRPRLP EGVVPKSKP QLNRYLTRWA PGSVKPIYKK GPRWNRVRMP VGSPLLRDNV CYSRTPWKLY H

UniProtKB: Large ribosomal subunit protein mL66

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Macromolecule #43: 39S ribosomal protein S30, mitochondrial

MacromoleculeName: 39S ribosomal protein S30, mitochondrial / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 45.204699 KDa
SequenceString: VARYPPIVAS MTADSKAARL RRIERWQATV HAAESVDEKL RILTKMQFMK YMVYPQTFAL NADRWYQYFT KTVFLSGLPP PPAEPEPEP EPEPEPALDL AALRAVACDC LLQEHFYLRR RRRVHRYEES EVISLPFLDQ LVSTLVGLLS PHNPALAAAA L DYRCPVHF ...String:
VARYPPIVAS MTADSKAARL RRIERWQATV HAAESVDEKL RILTKMQFMK YMVYPQTFAL NADRWYQYFT KTVFLSGLPP PPAEPEPEP EPEPEPALDL AALRAVACDC LLQEHFYLRR RRRVHRYEES EVISLPFLDQ LVSTLVGLLS PHNPALAAAA L DYRCPVHF YWVRGEEIIP RGHRRGRIDD LRYQIDDKPN NQIRISKQLA EFVPLDYSVP IEIPTIKCKP DKLPLFKRQY EN HIFVGSK TADPCCYGHT QFHLLPDKLR RERLLRQNCA DQIEVVFRAN AIASLFAWTG AQAMYQGFWS EADVTRPFVS QAV ITDGKY FSFFCYQLNT LALTTQADQN NPRKNICWGT QSKPLYETIE DNDVKGFNDD VLLQIVHFLL NRPK

UniProtKB: Large ribosomal subunit protein mL65

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Macromolecule #44: Mitochondrial assembly of ribosomal large subunit protein 1

MacromoleculeName: Mitochondrial assembly of ribosomal large subunit protein 1
type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 13.280472 KDa
SequenceString:
KFDIDMMVSL LRQENARDIC VIQVPPEMRY TDYFVIVSGT STRHLHAMAF YVVKMYKHLK CKRDPHVKIE GKDTDDWLCV DFGSMVIHL MLPETREIYE LEKLWTLRSY DD

UniProtKB: Mitochondrial assembly of ribosomal large subunit protein 1

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Macromolecule #45: MIEF1 upstream open reading frame protein

MacromoleculeName: MIEF1 upstream open reading frame protein / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 8.329592 KDa
SequenceString:
APWSREAVLS LYRALLRQGR QLRYTDRDFY FASIRREFRK NQKLEDAEAR ERQLEKGLVF LNGKLGRII

UniProtKB: Mitochondrial ribosome and complex I assembly factor AltMIEF1

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Macromolecule #46: Acyl carrier protein, mitochondrial

MacromoleculeName: Acyl carrier protein, mitochondrial / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 9.144511 KDa
SequenceString:
LTLEGIQDRV LYVLKLYDKI DPEKLSVNSH FMKDLGLDSL DQVEIIMAME DEFGFEIPDI DAEKLMCPQE IVDYIADKK

UniProtKB: Acyl carrier protein, mitochondrial

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Macromolecule #49: 39S ribosomal protein L36, mitochondrial

MacromoleculeName: 39S ribosomal protein L36, mitochondrial / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 4.768773 KDa
SequenceString:
FKNKTVLKKR CKDCYLVKRR GRWYVYCKTH PRHKQRQ

UniProtKB: Large ribosomal subunit protein bL36m

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Macromolecule #47: 16S ribosomal RNA

MacromoleculeName: 16S ribosomal RNA / type: rna / ID: 47 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 403.061188 KDa
SequenceString: GCUAAACCUA GCCCCAAACC CCCACCUUAC UACCACAACU UAGCCAAACC AUUUACAUAA AGUAUAGGCG AUAGAAAUUG AUGGCGCAA UAGAUAUAGU ACCGCAAGGG AAAGACAAGC AUAAUAUAGC AAGGACUAAC CCCUAUACCU UCUGCAUAAU G AAUUAACU ...String:
GCUAAACCUA GCCCCAAACC CCCACCUUAC UACCACAACU UAGCCAAACC AUUUACAUAA AGUAUAGGCG AUAGAAAUUG AUGGCGCAA UAGAUAUAGU ACCGCAAGGG AAAGACAAGC AUAAUAUAGC AAGGACUAAC CCCUAUACCU UCUGCAUAAU G AAUUAACU AGAAAUAACU UUGCAAGGAG AGCCAAAGCU AAGACCCCCG AAACCAGACG AGCUACCUAA GAACAGCUAA AA GAGCACA CCCGUCUAUG UAGCAAAAUA GUGGGAAGAU UUAUAGGUAG AGGCGACAAA CCUACCGAGC CUGGUGAUAG CUG GUUGUC CAAGAUAGAA UCUUAGUUCA ACUUUAAAUU UGCCCACAGA ACCAAAUCCC CUUGUAAAUU UAACUGUUAG UCCA AAGAG GAACAGCUCU UUGGACACUA GGAAAAAACC UUGUAGAGAG AGUAAAAAAU GAUCCCAAAC AUAUAACUGA ACUCC UCAC ACCCAAUUGG ACCAAUCUAU CAUAUAGAAG AACUAAUGUU AGUAUAAGUA ACAUGAAAAC AUUCUCCUCC GCAUAA GCC UGCGUCAGAU CUGACAAUUA ACAGCCCAAU AUCUACAAUC AACCAACAAG UUAUUACCCU CACUGUCAAC CCAACCA GG CAUGCUCAUA AGGAAAGGUU AAAAAAAGUA AAAGGAACUC GGCAAAUCUU ACCCCGCCUG UUUACCAAAA ACAUCACC U CUAGCAUCAC CAGUAUUAGA GGCACCGCCU CCUUAAAUAG GCUCCACGAG GGUUCAGCUG UCUCUUACUU UUAACCAGU GAAAUUGACC UGCCCGUGAG GCGGGCAUAA CACAGCAAGA CGAAGACCCU AUGGAGCUUU AAUUUAUUAA UGCAAAACCU GCAUUAAAA AUUUCGGUUG GGGCGACCUC GGAGCAGAAC CCAACCUCCG AGGCUAAGAC UUCACCAGUC AAAGCGAAGA U CCAAUAAC UUGACCAACG GAACAAGUUA CCCUAGGGCA AUCCUAUUCU AGAGUCCAUA UCAACAAUAG GGUUUACUGG AU CAGGACA UCCCGAUGGU GCAGCCGCUA UUAAAGGUUC GUUUGUUCAA CGAUUAAAGU CCUCGUGAUC UGAGUUCAGA CCG GAGUAA UCCAGGUCGG UUUCUAUCUA CUUUAUUCCU CCCUGUACGA AAGGACAAGA GAAAUAAGGC CUACUUCACA AAGC GCCUU CUAAAUGAUA UCAUCUCAAC UUAAUACCCA CACCCACCCA AGAACAGGGU U

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Macromolecule #48: mitochondrial tRNAVal

MacromoleculeName: mitochondrial tRNAVal / type: rna / ID: 48 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 19.530658 KDa
SequenceString:
AGAGUGUAGC UUAACACAAA GCACCCAACU UACACUUAGG AGAUUUCAAU UGACGCUCUG A

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Macromolecule #50: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 50 / Number of copies: 50 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #51: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 51 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #52: water

MacromoleculeName: water / type: ligand / ID: 52 / Number of copies: 14 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Average electron dose: 52.5 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.6 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 2709521
Startup modelType of model: OTHER / Details: Ab initio
Final reconstructionNumber classes used: 1 / Resolution.type: BY AUTHOR / Resolution: 2.89 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 224933
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final 3D classificationNumber classes: 5 / Software - Name: RELION (ver. 3.1)
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

5ool


Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-7qh7:
Cryo-EM structure of the human mtLSU assembly intermediate upon MRM2 depletion - class 4

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