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データを開く
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基本情報
登録情報 | ![]() | |||||||||
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タイトル | Cryo-EM structure of the human mtLSU assembly intermediate upon MRM2 depletion - class 4 | |||||||||
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![]() | Mitochondria / Ribosome / Assembly / Methyltransferase / MRM2 / RNA modification | |||||||||
機能・相同性 | ![]() negative regulation of mitochondrial translation / mitochondrial large ribosomal subunit assembly / protein lipoylation / Complex I biogenesis / negative regulation of ribosome biogenesis / Mitochondrial Fatty Acid Beta-Oxidation / Protein lipoylation / positive regulation of mitochondrial translation / Respiratory electron transport / rRNA import into mitochondrion ...negative regulation of mitochondrial translation / mitochondrial large ribosomal subunit assembly / protein lipoylation / Complex I biogenesis / negative regulation of ribosome biogenesis / Mitochondrial Fatty Acid Beta-Oxidation / Protein lipoylation / positive regulation of mitochondrial translation / Respiratory electron transport / rRNA import into mitochondrion / mitochondrial translational elongation / Mitochondrial translation elongation / Mitochondrial translation termination / Mitochondrial translation initiation / mitochondrial [2Fe-2S] assembly complex / iron-sulfur cluster assembly complex / mitochondrial large ribosomal subunit / mitochondrial fission / mitochondrial large ribosomal subunit binding / mitochondrial ribosome / 加水分解酵素; エステル加水分解酵素; 5'-リン酸モノエステル産生エンドリボヌクレアーゼ / mitochondrial small ribosomal subunit / [2Fe-2S] cluster assembly / mitochondrial translation / iron-sulfur cluster assembly / acyl binding / acyl carrier activity / ribosomal large subunit binding / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / proton motive force-driven mitochondrial ATP synthesis / respiratory chain complex I / RNA processing / Mitochondrial protein degradation / cellular response to leukemia inhibitory factor / aerobic respiration / ribosomal large subunit biogenesis / fatty acid binding / mitochondrial membrane / fatty acid biosynthetic process / double-stranded RNA binding / 5S rRNA binding / small ribosomal subunit rRNA binding / endonuclease activity / mitochondrial inner membrane / negative regulation of translation / rRNA binding / nuclear body / ribosome / structural constituent of ribosome / mitochondrial matrix / protein domain specific binding / translation / ribonucleoprotein complex / nucleotide binding / mRNA binding / apoptotic process / calcium ion binding / mitochondrion / extracellular space / RNA binding / nucleoplasm / nucleus / plasma membrane / cytosol 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.89 Å | |||||||||
![]() | Rebelo-Guiomar P / Pellegrino S / Dent KC / Warren AJ / Minczuk M | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: A late-stage assembly checkpoint of the human mitochondrial ribosome large subunit. 著者: Pedro Rebelo-Guiomar / Simone Pellegrino / Kyle C Dent / Aldema Sas-Chen / Leonor Miller-Fleming / Caterina Garone / Lindsey Van Haute / Jack F Rogan / Adam Dinan / Andrew E Firth / Byron ...著者: Pedro Rebelo-Guiomar / Simone Pellegrino / Kyle C Dent / Aldema Sas-Chen / Leonor Miller-Fleming / Caterina Garone / Lindsey Van Haute / Jack F Rogan / Adam Dinan / Andrew E Firth / Byron Andrews / Alexander J Whitworth / Schraga Schwartz / Alan J Warren / Michal Minczuk / ![]() ![]() ![]() 要旨: Many cellular processes, including ribosome biogenesis, are regulated through post-transcriptional RNA modifications. Here, a genome-wide analysis of the human mitochondrial transcriptome shows that ...Many cellular processes, including ribosome biogenesis, are regulated through post-transcriptional RNA modifications. Here, a genome-wide analysis of the human mitochondrial transcriptome shows that 2'-O-methylation is limited to residues of the mitoribosomal large subunit (mtLSU) 16S mt-rRNA, introduced by MRM1, MRM2 and MRM3, with the modifications installed by the latter two proteins being interdependent. MRM2 controls mitochondrial respiration by regulating mitoribosome biogenesis. In its absence, mtLSU particles (visualized by cryo-EM at the resolution of 2.6 Å) present disordered RNA domains, partial occupancy of bL36m and bound MALSU1:L0R8F8:mtACP anti-association module, allowing five mtLSU biogenesis intermediates with different intersubunit interface configurations to be placed along the assembly pathway. However, mitoribosome biogenesis does not depend on the methyltransferase activity of MRM2. Disruption of the MRM2 Drosophila melanogaster orthologue leads to mitochondria-related developmental arrest. This work identifies a key checkpoint during mtLSU assembly, essential to maintain mitochondrial homeostasis. | |||||||||
履歴 |
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構造の表示
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 139.8 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 62.3 KB 62.3 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 12.7 KB | 表示 | ![]() |
画像 | ![]() | 77.6 KB | ||
Filedesc metadata | ![]() | 13.1 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 7qh7MC ![]() 7qh6C C: 同じ文献を引用 ( M: このマップから作成された原子モデル |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.06 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : Human mitochondrial ribosome large subunit
+超分子 #1: Human mitochondrial ribosome large subunit
+分子 #1: 39S ribosomal protein L2, mitochondrial
+分子 #2: 39S ribosomal protein L3, mitochondrial
+分子 #3: 39S ribosomal protein L4, mitochondrial
+分子 #4: 39S ribosomal protein L9, mitochondrial
+分子 #5: 39S ribosomal protein L10, mitochondrial
+分子 #6: 39S ribosomal protein L13, mitochondrial
+分子 #7: 39S ribosomal protein L14, mitochondrial
+分子 #8: 39S ribosomal protein L15, mitochondrial
+分子 #9: 39S ribosomal protein L16, mitochondrial
+分子 #10: 39S ribosomal protein L17, mitochondrial
+分子 #11: 39S ribosomal protein L18, mitochondrial
+分子 #12: 39S ribosomal protein L19, mitochondrial
+分子 #13: 39S ribosomal protein L20, mitochondrial
+分子 #14: 39S ribosomal protein L21, mitochondrial
+分子 #15: 39S ribosomal protein L22, mitochondrial
+分子 #16: 39S ribosomal protein L23, mitochondrial
+分子 #17: 39S ribosomal protein L24, mitochondrial
+分子 #18: 39S ribosomal protein L27, mitochondrial
+分子 #19: 39S ribosomal protein L28, mitochondrial
+分子 #20: 39S ribosomal protein L47, mitochondrial
+分子 #21: 39S ribosomal protein L30, mitochondrial
+分子 #22: 39S ribosomal protein L32, mitochondrial
+分子 #23: 39S ribosomal protein L33, mitochondrial
+分子 #24: 39S ribosomal protein L34, mitochondrial
+分子 #25: 39S ribosomal protein L35, mitochondrial
+分子 #26: 39S ribosomal protein L37, mitochondrial
+分子 #27: 39S ribosomal protein L38, mitochondrial
+分子 #28: 39S ribosomal protein L39, mitochondrial
+分子 #29: 39S ribosomal protein L41, mitochondrial
+分子 #30: 39S ribosomal protein L42, mitochondrial
+分子 #31: 39S ribosomal protein L43, mitochondrial
+分子 #32: 39S ribosomal protein L44, mitochondrial
+分子 #33: 39S ribosomal protein L45, mitochondrial
+分子 #34: 39S ribosomal protein L48, mitochondrial
+分子 #35: 39S ribosomal protein L49, mitochondrial
+分子 #36: 39S ribosomal protein L50, mitochondrial
+分子 #37: 39S ribosomal protein L51, mitochondrial
+分子 #38: 39S ribosomal protein L52, mitochondrial
+分子 #39: Ribosomal protein 63, mitochondrial
+分子 #40: Peptidyl-tRNA hydrolase ICT1, mitochondrial
+分子 #41: Growth arrest and DNA damage-inducible proteins-interacting protein 1
+分子 #42: 39S ribosomal protein S18a, mitochondrial
+分子 #43: 39S ribosomal protein S30, mitochondrial
+分子 #44: Mitochondrial assembly of ribosomal large subunit protein 1
+分子 #45: MIEF1 upstream open reading frame protein
+分子 #46: Acyl carrier protein, mitochondrial
+分子 #49: 39S ribosomal protein L36, mitochondrial
+分子 #47: 16S ribosomal RNA
+分子 #48: mitochondrial tRNAVal
+分子 #50: MAGNESIUM ION
+分子 #51: ZINC ION
+分子 #52: water
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.4 |
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グリッド | モデル: Quantifoil R2/2 / 材質: COPPER / メッシュ: 300 / 前処理 - タイプ: GLOW DISCHARGE |
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277.15 K / 装置: FEI VITROBOT MARK IV |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 検出モード: INTEGRATING / 平均電子線量: 52.5 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.6 µm / 最小 デフォーカス(公称値): 1.0 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |