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- EMDB-13936: Cryo-EM structure of the ribosome from Encephalitozoon cuniculi -

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Basic information

Entry
Database: EMDB / ID: EMD-13936
TitleCryo-EM structure of the ribosome from Encephalitozoon cuniculi
Map dataconsensus map
Sample
  • Complex: Ribosome from Encephalitozoon cuniculi
    • Complex: large subunit
      • Protein or peptide: x 42 types
      • RNA: x 1 types
    • Complex: small subunit
      • RNA: x 2 types
      • Protein or peptide: x 31 types
    • Protein or peptide: x 1 types
  • Ligand: x 3 types
Function / homology
Function and homology information


rRNA processing / large ribosomal subunit / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / rRNA binding / ribosome / structural constituent of ribosome ...rRNA processing / large ribosomal subunit / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / rRNA binding / ribosome / structural constituent of ribosome / translation / nucleolus / RNA binding / nucleus / metal ion binding / cytoplasm
Similarity search - Function
CXXC motif containing zinc binding protein, eukaryotic / CXXC motif containing zinc binding protein, eukaryotic / Ribosomal protein L30e / Ribosomal L15/L27a, N-terminal / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S12e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S2, eukaryotic ...CXXC motif containing zinc binding protein, eukaryotic / CXXC motif containing zinc binding protein, eukaryotic / Ribosomal protein L30e / Ribosomal L15/L27a, N-terminal / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S12e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S2, eukaryotic / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein L29e / Ribosomal L29e protein family / S27a-like superfamily / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein L1 / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein L10e / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L19, eukaryotic / Ribosomal protein S19e / Ribosomal protein S19e / Ribosomal_S19e / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein S19A/S15e / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal L40e family / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein S6, eukaryotic / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S4e, N-terminal / RS4NT (NUC023) domain / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Eukaryotic Ribosomal Protein L27, KOW domain / 40S ribosomal protein S11, N-terminal / Ribosomal_S17 N-terminal / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal family S4e / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal protein L34Ae / Ribosomal protein L34e / Ribosomal protein S24e / Ribosomal protein S23, eukaryotic/archaeal / 60S ribosomal protein L19 / Ribosomal protein S24e / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S6e signature. / Ribosomal protein S27 / Ribosomal protein L30/YlxQ / Ribosomal protein S27 / Ribosomal Protein L6, KOW domain / Ribosomal protein S8e / Ribosomal protein L7A/L8 / Ribosomal protein L6e / Ribosomal protein S3Ae / Ribosomal S3Ae family / Ribosomal S3Ae family / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein S28e / Ribosomal protein S28e / 60S ribosomal protein L6E / Ribosomal protein S6e / Ribosomal protein L18e / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S6e / Ribosomal protein S6e / Ribosomal protein L37ae
Similarity search - Domain/homology
ECU06_1135 protein / 60S ribosomal protein L29 / ECU06_1215 protein / ECU11_0225 protein / Guanine nucleotide binding protein beta subunit / 60S ribosomal protein L27a / 60S ribosomal protein L3 / 40S RIBOSOMAL PROTEIN S15A (S22 in yeast) / 40S RIBOSOMAL PROTEIN S28 / 40S ribosomal protein S3 ...ECU06_1135 protein / 60S ribosomal protein L29 / ECU06_1215 protein / ECU11_0225 protein / Guanine nucleotide binding protein beta subunit / 60S ribosomal protein L27a / 60S ribosomal protein L3 / 40S RIBOSOMAL PROTEIN S15A (S22 in yeast) / 40S RIBOSOMAL PROTEIN S28 / 40S ribosomal protein S3 / 60S ribosomal protein L39 / 40S ribosomal protein S19 / Ribosomal protein L15 / 40S ribosomal protein S7 / 40S RIBOSOMAL PROTEIN S20 / 40S RIBOSOMAL PROTEIN S24 / 60S ribosomal protein L44 / 40S ribosomal protein S23 / 60S RIBOSOMAL PROTEIN L23A / 60S RIBOSOMAL PROTEIN L6 / 60S ribosomal protein L10 / 60S ribosomal protein L23 / 40S ribosomal protein S13 / 40S ribosomal protein S4 / 60S RIBOSOMAL PROTEIN L4 / 60S ribosomal protein L20 / 60S RIBOSOMAL PROTEIN L26 / RIBOSOMAL PROTEIN S15 / 40S RIBOSOMAL PROTEIN S2 / 60S ribosomal protein L37 / 60S RIBOSOMAL PROTEIN L17 / UBIQUITIN/ L40 RIBOSOMAL PROTEIN FUSION / 60S RIBOSOMAL PROTEIN L37A (L43) / 40S ribosomal protein S26 / 60S ribosomal protein L36 / 40S ribosomal protein S18 / 60S RIBOSOMAL PROTEIN L19 / 60S RIBOSOMAL PROTEIN L5 / 60S RIBOSOMAL PROTEIN L30 / 40S ribosomal protein S9 / 60S ribosomal protein L21 / 40S ribosomal protein S6 / 60S ribosomal protein L1 / 40S ribosomal protein S1 / 60S RIBOSOMAL PROTEIN L13A (L16) / 40S RIBOSOMAL PROTEIN S10 / 60S ribosomal protein L32 / 40S RIBOSOMAL PROTEIN S27 / 60S ribosomal protein L22 / 40S RIBOSOMAL PROTEIN S11 / 40S ribosomal protein S0 / 60S RIBOSOMAL PROTEIN L27 / 40S ribosomal protein S5 / 60S RIBOSOMAL PROTEIN L18 / 60S ribosomal protein L7 / 60S ribosomal protein L34 / 40S ribosomal protein S14 / 60S RIBOSOMAL PROTEIN L13 / 40S RIBOSOMAL PROTEIN S16 / 60S ribosomal protein L31 / 60S RIBOSOMAL PROTEIN L35A (L33) / 60S RIBOSOMAL PROTEIN L9 / 40S ribosomal protein S17 / 60S ribosomal protein L7a / 60S ribosomal protein L11 / 40S RIBOSOMAL PROTEIN S12 / 60S ribosomal protein L8 / 60S ribosomal protein L35-1 / 40S ribosomal protein S25 / Similarity to 60S RIBOSOMAL PROTEIN L24 / Similarity to monoubiquitin/carboxy-extension protein fusion / 40S ribosomal protein S8 / Uncharacterized protein ECU01_0250
Similarity search - Component
Biological speciesEncephalitozoon cuniculi (fungus) / Encephalitozoon cuniculi GB-M1 (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.7 Å
AuthorsNicholson D / Ranson NA / Melnikov SV
Funding support United Kingdom, 6 items
OrganizationGrant numberCountry
Wellcome Trust203743/Z/16/Z United Kingdom
Medical Research Council (MRC, United Kingdom)G0901526 United Kingdom
Medical Research Council (MRC, United Kingdom)MR/N009738/1 United Kingdom
H2020 Marie Curie Actions of the European Commission97617 United Kingdom
H2020 Marie Curie Actions of the European Commission895166 United Kingdom
Wellcome Trust108466/Z/15/Z United Kingdom
CitationJournal: Nat Commun / Year: 2022
Title: Adaptation to genome decay in the structure of the smallest eukaryotic ribosome.
Authors: David Nicholson / Marco Salamina / Johan Panek / Karla Helena-Bueno / Charlotte R Brown / Robert P Hirt / Neil A Ranson / Sergey V Melnikov /
Abstract: The evolution of microbial parasites involves the counterplay between natural selection forcing parasites to improve and genetic drifts forcing parasites to lose genes and accumulate deleterious ...The evolution of microbial parasites involves the counterplay between natural selection forcing parasites to improve and genetic drifts forcing parasites to lose genes and accumulate deleterious mutations. Here, to understand how this counterplay occurs at the scale of individual macromolecules, we describe cryo-EM structure of ribosomes from Encephalitozoon cuniculi, a eukaryote with one of the smallest genomes in nature. The extreme rRNA reduction in E. cuniculi ribosomes is accompanied with unparalleled structural changes, such as the evolution of previously unknown molten rRNA linkers and bulgeless rRNA. Furthermore, E. cuniculi ribosomes withstand the loss of rRNA and protein segments by evolving an ability to use small molecules as structural mimics of degenerated rRNA and protein segments. Overall, we show that the molecular structures long viewed as reduced, degenerated, and suffering from debilitating mutations possess an array of compensatory mechanisms that allow them to remain active despite the extreme molecular reduction.
History
DepositionDec 3, 2021-
Header (metadata) releaseFeb 9, 2022-
Map releaseFeb 9, 2022-
UpdateFeb 9, 2022-
Current statusFeb 9, 2022Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.05
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.05
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-7qep
  • Surface level: 0.05
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_13936.png

Downloads & links

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Map

FileDownload / File: emd_13936.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationconsensus map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.86 Å/pix.
x 512 pix.
= 440.832 Å		0.86 Å/pix.
x 512 pix.
= 440.832 Å		0.86 Å/pix.
x 512 pix.
= 440.832 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.861 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.05 / Movie #1: 0.05
Minimum - Maximum-0.41054884 - 0.92765087
Average (Standard dev.)0.00022292779 (±0.016516855)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 440.832 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.8610.8610.861
M x/y/z512512512
origin x/y/z0.0000.0000.000
length x/y/z440.832440.832440.832
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS512512512
D min/max/mean-0.4110.9280.000

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Supplemental data

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Mask #1

Fileemd_13936_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: small subunit body map

Fileemd_13936_additional_1.map
Annotationsmall subunit body map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: small subunit head map

Fileemd_13936_additional_2.map
Annotationsmall subunit head map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: consensus half map 1

Fileemd_13936_half_map_1.map
Annotationconsensus half map 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: consensus half map 2

Fileemd_13936_half_map_2.map
Annotationconsensus half map 2
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Ribosome from Encephalitozoon cuniculi

EntireName: Ribosome from Encephalitozoon cuniculi
Components
  • Complex: Ribosome from Encephalitozoon cuniculi
    • Complex: large subunit
      • Protein or peptide: Guanine nucleotide binding protein beta subunit
      • RNA: 5.8S-23S ribosomal RNA
      • Protein or peptide: Similarity to monoubiquitin/carboxy-extension protein fusion
      • Protein or peptide: 60S ribosomal protein L1
      • Protein or peptide: 60S ribosomal protein L8
      • Protein or peptide: 60S ribosomal protein L3
      • Protein or peptide: 60S RIBOSOMAL PROTEIN L4
      • Protein or peptide: 60S RIBOSOMAL PROTEIN L5
      • Protein or peptide: 60S RIBOSOMAL PROTEIN L6
      • Protein or peptide: 60S ribosomal protein L7
      • Protein or peptide: 60S ribosomal protein L7a
      • Protein or peptide: 60S RIBOSOMAL PROTEIN L9
      • Protein or peptide: 60S ribosomal protein L10
      • Protein or peptide: 60S ribosomal protein L11
      • Protein or peptide: 60S RIBOSOMAL PROTEIN L13
      • Protein or peptide: ECU06_1215 protein
      • Protein or peptide: Ribosomal protein L15
      • Protein or peptide: 60S RIBOSOMAL PROTEIN L13A (L16)
      • Protein or peptide: 60S RIBOSOMAL PROTEIN L17
      • Protein or peptide: 60S RIBOSOMAL PROTEIN L18
      • Protein or peptide: Uncharacterized protein ECU01_0250
      • Protein or peptide: ECU06_1135 protein
      • Protein or peptide: 60S ribosomal protein L20
      • Protein or peptide: 60S ribosomal protein L21
      • Protein or peptide: 60S ribosomal protein L22
      • Protein or peptide: 60S ribosomal protein L23
      • Protein or peptide: Similarity to 60S RIBOSOMAL PROTEIN L24
      • Protein or peptide: 60S RIBOSOMAL PROTEIN L23A
      • Protein or peptide: 60S RIBOSOMAL PROTEIN L26
      • Protein or peptide: 60S RIBOSOMAL PROTEIN L27
      • Protein or peptide: 60S ribosomal protein L27a
      • Protein or peptide: 60S ribosomal protein L29
      • Protein or peptide: 60S RIBOSOMAL PROTEIN L30
      • Protein or peptide: 60S ribosomal protein L31
      • Protein or peptide: 60S ribosomal protein L32
      • Protein or peptide: 60S RIBOSOMAL PROTEIN L35A (L33)
      • Protein or peptide: 60S ribosomal protein L34
      • Protein or peptide: 60S ribosomal protein L35-1
      • Protein or peptide: 60S ribosomal protein L36
      • Protein or peptide: 60S ribosomal protein L37
      • Protein or peptide: 60S ribosomal protein L39
      • Protein or peptide: UBIQUITIN/ L40 RIBOSOMAL PROTEIN FUSION
      • Protein or peptide: 60S ribosomal protein L44
    • Complex: small subunit
      • RNA: 5S ribosomal RNA
      • RNA: 18S ribosomal RNA
      • Protein or peptide: 40S RIBOSOMAL PROTEIN S10
      • Protein or peptide: 40S RIBOSOMAL PROTEIN S11
      • Protein or peptide: 40S RIBOSOMAL PROTEIN S12
      • Protein or peptide: 40S ribosomal protein S13
      • Protein or peptide: 40S ribosomal protein S14
      • Protein or peptide: RIBOSOMAL PROTEIN S15
      • Protein or peptide: 40S RIBOSOMAL PROTEIN S16
      • Protein or peptide: 40S ribosomal protein S17
      • Protein or peptide: 40S ribosomal protein S18
      • Protein or peptide: 40S ribosomal protein S19
      • Protein or peptide: 40S RIBOSOMAL PROTEIN S20
      • Protein or peptide: ECU11_0225 protein
      • Protein or peptide: 40S RIBOSOMAL PROTEIN S15A (S22 in yeast)
      • Protein or peptide: 40S ribosomal protein S23
      • Protein or peptide: 40S RIBOSOMAL PROTEIN S24
      • Protein or peptide: 40S ribosomal protein S25
      • Protein or peptide: 40S ribosomal protein S26
      • Protein or peptide: 40S RIBOSOMAL PROTEIN S27
      • Protein or peptide: 40S RIBOSOMAL PROTEIN S28
      • Protein or peptide: 40S ribosomal protein S29
      • Protein or peptide: 60S RIBOSOMAL PROTEIN L37A (L43)
      • Protein or peptide: 40S ribosomal protein S0
      • Protein or peptide: 40S ribosomal protein S1
      • Protein or peptide: 40S RIBOSOMAL PROTEIN S2
      • Protein or peptide: 40S ribosomal protein S3
      • Protein or peptide: 40S ribosomal protein S4
      • Protein or peptide: 40S ribosomal protein S5
      • Protein or peptide: 40S ribosomal protein S6
      • Protein or peptide: 40S ribosomal protein S7
      • Protein or peptide: 40S ribosomal protein S8
      • Protein or peptide: 40S ribosomal protein S9
    • Protein or peptide: 60S RIBOSOMAL PROTEIN L19
  • Ligand: ZINC ION
  • Ligand: ADENOSINE MONOPHOSPHATE
  • Ligand: SPERMIDINE

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Supramolecule #1: Ribosome from Encephalitozoon cuniculi

SupramoleculeName: Ribosome from Encephalitozoon cuniculi / type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: #1-#77
Source (natural)Organism: Encephalitozoon cuniculi (fungus) / Strain: strain II

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Supramolecule #2: large subunit

SupramoleculeName: large subunit / type: complex / Chimera: Yes / ID: 2 / Parent: 1 / Macromolecule list: #1-#2, #25-#42, #44-#66
Source (natural)Organism: Encephalitozoon cuniculi (fungus) / Strain: strain II

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Supramolecule #3: small subunit

SupramoleculeName: small subunit / type: complex / Chimera: Yes / ID: 3 / Parent: 1 / Macromolecule list: #3-#24, #67-#77
Source (natural)Organism: Encephalitozoon cuniculi (fungus) / Strain: strain II

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Macromolecule #1: Guanine nucleotide binding protein beta subunit

MacromoleculeName: Guanine nucleotide binding protein beta subunit / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 36.828836 KDa
SequenceString: MSSAELEVLE YLNGHKDEVK GLKIVRVSGR EILYSSSRDK KVFSWDLHSK IDSEFGKILK LYDGGHSKRI NGLDVSKDGN MMVTVGSDG IGRIWDTESK KSILLEGHSR DVLCVSINSN DTKIVTGSVD RTMNLYNTKG DLVLKMGRDM EMMHRGWINC V TFHPTEES ...String:
MSSAELEVLE YLNGHKDEVK GLKIVRVSGR EILYSSSRDK KVFSWDLHSK IDSEFGKILK LYDGGHSKRI NGLDVSKDGN MMVTVGSDG IGRIWDTESK KSILLEGHSR DVLCVSINSN DTKIVTGSVD RTMNLYNTKG DLVLKMGRDM EMMHRGWINC V TFHPTEES ILASGSADGT VKIWDLDTQE HLQTYLGGAY VDYEKAKEKK TSPVDYDESK SVTAMAFSKD GSILTYGEKS GK MYLVKVD SKECIQSFDA IVPVRSIAVG ETEPVIALGT DESVIIWETI SSRVIASYNL KEIGNGVRCL SLAFSGSTLY CGL STGAIV PLELRKSD

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Macromolecule #5: 40S RIBOSOMAL PROTEIN S10

MacromoleculeName: 40S RIBOSOMAL PROTEIN S10 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 11.167082 KDa
SequenceString:
MLVPTANKYK IKRRLLEKKA LLLEDTVLGN HRDLEIPNLH LKIFMKTMIS YGYVQRIHVW RHSYFLLTPL GESRLREELV FTDEGVASQ EAPAVSA

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Macromolecule #6: 40S RIBOSOMAL PROTEIN S11

MacromoleculeName: 40S RIBOSOMAL PROTEIN S11 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 17.78891 KDa
SequenceString:
MEEVRKLKVF PRQTGVCAKP YASHEEQPKR RVNAEVCGFK APETAYTRQY IDKKCPFTGD VNVRGRLFKG VVKKMKAEKT IVVVANYVH YSKKYKRYSR RHTNFSVHMS PCFEGMINVG DTVICGETRP LSKTKSSVVL GYIKKALPGS FKVLESI

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Macromolecule #7: 40S RIBOSOMAL PROTEIN S12

MacromoleculeName: 40S RIBOSOMAL PROTEIN S12 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 14.518135 KDa
SequenceString:
MSEMQEPMME PEMTLQEALS KVCKVSRTYC KLSRGAKETT KKMLADKMSF VMVAENAEPR ISKLVMALAK KKNIPVISIG SCLELGRIV GVENVSSSGK VRSKGCCVAG VQDYCEQTSE AGFVQAALLK GISSQ

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Macromolecule #8: 40S ribosomal protein S13

MacromoleculeName: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 16.858637 KDa
SequenceString:
MAKMHSSGKG RSGSVKPYAT AFPTWLTKSV DEIKSDVIQM GNKGVPAPDI GTRLRDEYGI GKASDVLGES ITRFLQRNGV VPKIPHDLE SLVHRANTLR SHLNIYRKDN SAKYRLILVS SRMYRVARYY KRKMRIPGNW KPKLVELNK

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Macromolecule #9: 40S ribosomal protein S14

MacromoleculeName: 40S ribosomal protein S14 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 14.423893 KDa
SequenceString:
MTEQIESMEL AAVAHIKATK NDTFVHITDM TGSETIAKIT GGMRVKAQRD EGSPYAAMLA AQDVATKILG RGVKVLHFKL RGAGGVKPM ALGPGAQTAI RTLIRAGLRV GRIEDVTPVA RDRVRKRGGH RGRRV

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Macromolecule #10: RIBOSOMAL PROTEIN S15

MacromoleculeName: RIBOSOMAL PROTEIN S15 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 16.383311 KDa
SequenceString:
MKGDNEVSKK KRTFRRYTYR GVPVEELEKM PFSEVAKLFP SRLRRRVRRG LSSREVELIK GCIAAKAACV SSSSKPEMVR THARSAIIW PAMIGALVGV HVGNGYLPVE IKPEMVGFVL SDFAPTRVTP KHGRPGIGAS SSSKFVPLK

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Macromolecule #11: 40S RIBOSOMAL PROTEIN S16

MacromoleculeName: 40S RIBOSOMAL PROTEIN S16 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 16.602551 KDa
SequenceString:
MSRSVKSVTT QGKKKRAVAT CVCTQTGEFS IRINKVPFKL ITNTLMIAKL KEIICIVEQA NIKDLSFDIT CKIGGDVSRV YAVRMAFAK AILAFYGTYF DEWKKQEIQK RLLDFDRFSL VADSRKREPK KFGGPGARAR YQKSYR

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Macromolecule #12: 40S ribosomal protein S17

MacromoleculeName: 40S ribosomal protein S17 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 14.057334 KDa
SequenceString:
MGCVRNKIVK RAARTIAEKY FQRLDSTFDH NLLVVQDVAV VQSKKLKNEI AGYLTSLYKK ILKGTYNKVY IKSHEEERER KENVIPKES MLDVDCVEVD DVTMEMIKRY GYEGNFKVYG M

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Macromolecule #13: 40S ribosomal protein S18

MacromoleculeName: 40S ribosomal protein S18 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 17.43135 KDa
SequenceString:
MNYTYDPAEV QYIVRIHNTN IDGTKRIPFA LTKIRGIGIR IATAICKRLG IDLRKRAGEM GEDEMKRISD AILDPASVGI PESYMNHQR DIIDGTTSHL IGTRLDADLR MMIERGKKNK RIRAYRLDVG LKVRGQRTKS NGRRGRSMGV SRKK

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Macromolecule #14: 40S ribosomal protein S19

MacromoleculeName: 40S ribosomal protein S19 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 15.806159 KDa
SequenceString:
MIEGIYEVMP EKFNEALKSY LKSTNEVVPL QDHDIMKTGE GREQAPIEED WYFTRMASIV RQISIKGAVT SEFLAKRYGS LKNRGCRPS KYVGAYPEIG ESVLENLKNM GWINEHPKDM LTEKGKTIVR EIIEKVRE

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Macromolecule #15: 40S RIBOSOMAL PROTEIN S20

MacromoleculeName: 40S RIBOSOMAL PROTEIN S20 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 14.236458 KDa
SequenceString:
MDVEMKKQDI DVASTMETMK VCIDMASLEE KQINKVCREF YEYGKTFIDD LQRETVLPKK VESITTLRNP SGQGTKKWSR YKMIVHLRN FYLEATHQQL EKVVQFLRNF PHVEINLSIQ N

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Macromolecule #16: ECU11_0225 protein

MacromoleculeName: ECU11_0225 protein / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 7.661769 KDa
SequenceString:
MTFERRICSL GKTPIASSDK ASVQIVLAVL DQYGQVTGET TIYDIGGTVR RAGMGDELLL EKIRMEEYGA

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Macromolecule #17: 40S RIBOSOMAL PROTEIN S15A (S22 in yeast)

MacromoleculeName: 40S RIBOSOMAL PROTEIN S15A (S22 in yeast) / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 14.582005 KDa
SequenceString:
MSAALANMCK AINNASRAGK RQLLLRFSTE EMRMFLLQML RHGYISGFSY IHDKRSGKSI IDLNGRLNRC GAISPNYIVK RDGIEDFRT RLLPARQFGH VLFNTSKGIL DHKECLTENV GGKILGYFY

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Macromolecule #18: 40S ribosomal protein S23

MacromoleculeName: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 15.345015 KDa
SequenceString:
MPGLFSANNL IKSRKAKRLA DTAYRKRALG TKYKHSVLGR APQAKAIVLE KIGVEAKQPN SAIRKAVRCQ LIATGKKITA FVPYDGSVT YIESNDEVTV EGFGKKGRSV GDIPGIRFKV CKVQNVSLHA IFTGKKEKPS R

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Macromolecule #19: 40S RIBOSOMAL PROTEIN S24

MacromoleculeName: 40S RIBOSOMAL PROTEIN S24 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 15.529335 KDa
SequenceString:
MSLELEIADL RDNRLLSRKE LDVVAYHPGM PIPSKEEIRE KISELYQTKK DNVVVMDLKN RYGTHRTTLK AKIYSSIEVL SNIEKKHIV AKLTGKEFHV TPRRVRKDER KKRYKIFGTL KRAMKKAERK NK

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Macromolecule #20: 40S ribosomal protein S25

MacromoleculeName: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 12.355396 KDa
SequenceString:
MVKKIQESKE KKALKAASGT RKDKKKWGDG RKKEEVRRAV TVSEELLAKV RKDVGRASVV TRYMIGSRYN LNLGVAENVL RHLSNEGVV QQVLGNRRMT IYAGCRAQEQ

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Macromolecule #21: 40S ribosomal protein S26

MacromoleculeName: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 11.902758 KDa
SequenceString:
MRRNHGRGKK NRGSAGSIQC DKCGSVTPKD KAIKRFRIQS LIEQASFDDL KQATIYDVFE VPRMGYKSQF CVSCACHAKI VRVRSSQAR KIRYGFNPNR VASAHN

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Macromolecule #22: 40S RIBOSOMAL PROTEIN S27

MacromoleculeName: 40S RIBOSOMAL PROTEIN S27 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 9.434813 KDa
SequenceString:
MASDLAFPTR EKVGSTYKKD RLYREGNGFF MYVRCGGCRH TTCCYSHSQT NISCPGCGDI ILTSSGGRAV VSPNCEFKKI IRNID

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Macromolecule #23: 40S RIBOSOMAL PROTEIN S28

MacromoleculeName: 40S RIBOSOMAL PROTEIN S28 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 7.459593 KDa
SequenceString:
MEEQQEFFGE VTHVLGRTGG SGLLTQVKME LMHNKRTIQR AVKGPVAVGD IIEILECERE HRRTR

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Macromolecule #24: 40S ribosomal protein S29

MacromoleculeName: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 7.637959 KDa
SequenceString:
MSFEVLFDVP VKKNEPSGPH SHRKPFGKGS RSCVSCYTFR GIIRKLMMCR RCFREYAGDI GFAIYD

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Macromolecule #25: Similarity to monoubiquitin/carboxy-extension protein fusion

MacromoleculeName: Similarity to monoubiquitin/carboxy-extension protein fusion
type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 17.141877 KDa
SequenceString:
MQIFVKTLTG KTITLEVEPG DTIDNVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGGAKK KKKDFSTPK KVKEPRKKRG LSWLDNFTVT DDKVKMNGVS CEVCGTEVFC GKIQSGFKCG RCYRVYDKDI ASH

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Macromolecule #26: 60S ribosomal protein L1

MacromoleculeName: 60S ribosomal protein L1 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 24.752602 KDa
SequenceString: MSKQAEEEQL AFLDSGRIIG IINQLKESED PEKIVTIQIQ VNLKGFDPRK DNKVSKDMVL PYRVRSLDKT IVIADEAHVK VCIDANLPY VPIDEISGDD KKDIRESVLK KNKFFILCPG YNKIYQLKNI LRCGKTPHIL RNGDDINAVF ETGKKSCKLR I QDDFSVTS ...String:
MSKQAEEEQL AFLDSGRIIG IINQLKESED PEKIVTIQIQ VNLKGFDPRK DNKVSKDMVL PYRVRSLDKT IVIADEAHVK VCIDANLPY VPIDEISGDD KKDIRESVLK KNKFFILCPG YNKIYQLKNI LRCGKTPHIL RNGDDINAVF ETGKKSCKLR I QDDFSVTS FTVGHTGMDS EHIYENIKVG MGLLVSYLKN GSQNLKGVMI KTDQSPPVTL Y

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Macromolecule #27: 60S ribosomal protein L8

MacromoleculeName: 60S ribosomal protein L8 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 25.911061 KDa
SequenceString: MGKITRRTRE AKKKQRQPIV KVCLSYPIIP QVEEGEVIDI VHERSRGAPV AIISFKEDDC MVPASEGMYT GQKILIGDDA PIDIGNITK IKNVPEGMAV NSVESVYGDG GTFAMVNGSY SLVVNHRKET NETVIKIPSG KKLTVSSECR CIVGVVAGGG I HDKPLLKA ...String:
MGKITRRTRE AKKKQRQPIV KVCLSYPIIP QVEEGEVIDI VHERSRGAPV AIISFKEDDC MVPASEGMYT GQKILIGDDA PIDIGNITK IKNVPEGMAV NSVESVYGDG GTFAMVNGSY SLVVNHRKET NETVIKIPSG KKLTVSSECR CIVGVVAGGG I HDKPLLKA SVAHYKAKAR GHVFPRVRGV AMNPVEHIHG GGNHQHVGKP TTVSKKDISQ EQKIGLVGAR RTGYRVGSRK L

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Macromolecule #28: 60S ribosomal protein L3

MacromoleculeName: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 42.555711 KDa
SequenceString: MSCRKFNAPR HGSLQFCPRK RSKTIRPAAG AFPADDRSQP VHLTGFMAYK AGMTHVVRTK TQVAKNKQLS REIMDAVTVL EAPPMVVYG IVGYEKTVTG LRRLPIVTAA YVSDGVLRRM FGNRYASKES AGQFCKGSVC ESRVEMIKER AHCVRVLVQT Q PTLIKGLG ...String:
MSCRKFNAPR HGSLQFCPRK RSKTIRPAAG AFPADDRSQP VHLTGFMAYK AGMTHVVRTK TQVAKNKQLS REIMDAVTVL EAPPMVVYG IVGYEKTVTG LRRLPIVTAA YVSDGVLRRM FGNRYASKES AGQFCKGSVC ESRVEMIKER AHCVRVLVQT Q PTLIKGLG LKKAHIAEIQ VNGGSISEKV EWALGRLEKE IAIGEVFGVN ENIDTIGVTK GKGFQGTVKR FGVRKQPRKS RK GIRKVAC IGAWHPSRVM YSIARAGQMG FHRRTEKNKR VYMIGNGSSN IKTEFDLTEK PISPMGGFPH YGEVRNDFIM VKG AVVGAR KRVVTLRKSL LRQRAGEELV IKFVDTSSKI GHGRFQTSAE KKAFYGARKA DIAAEIH

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Macromolecule #29: 60S RIBOSOMAL PROTEIN L4

MacromoleculeName: 60S RIBOSOMAL PROTEIN L4 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 37.767617 KDa
SequenceString: MKQQINCYEA DGVTVKKTLD LPEVFSVPIR QDLVLKTYSL YRMKTRQPYA VSKNAGMQHS AESWGTGRAI ARVPRVKGGG TRRAGQGAF ANFCRKGRMA HPTKTHRRWA RKVVENTKKI AEAMAVAASA QASLVEARGH RISDVKMLPI IVSDDIAKAR K TKDAVEIL ...String:
MKQQINCYEA DGVTVKKTLD LPEVFSVPIR QDLVLKTYSL YRMKTRQPYA VSKNAGMQHS AESWGTGRAI ARVPRVKGGG TRRAGQGAF ANFCRKGRMA HPTKTHRRWA RKVVENTKKI AEAMAVAASA QASLVEARGH RISDVKMLPI IVSDDIAKAR K TKDAVEIL RNLNMTEELK RVEKSRGVRC GKGKMRNRRY TQRKGILIVH GESSEMLGFR NIRGVDQMSI DSLDLLELAP GG VAGRLII WTESAFEKLD KIFGGVEREA ELTPGFKLPN RIVTHDDLEE LFYSDAVQAI LDEPLFVGRT SVKLDDEEME ERL RFIRKF DASAIRSTN

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Macromolecule #30: 60S RIBOSOMAL PROTEIN L5

MacromoleculeName: 60S RIBOSOMAL PROTEIN L5 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 32.77025 KDa
SequenceString: MSNAKGTRNY HKNFQVKRRR RREGKTDYKH RTNMIRQDSN SCGSVKHRLV VRITGSRVIC QIVAAYMDGD RVMVYADSRE LERYGINFG ATNYSAAYAT GFLIGRRALT AMSLDEVYRP KEIDGTYSVT KDIDGEKKAP RVFLDIGLAR STRGARVFGA L KGASDAGL ...String:
MSNAKGTRNY HKNFQVKRRR RREGKTDYKH RTNMIRQDSN SCGSVKHRLV VRITGSRVIC QIVAAYMDGD RVMVYADSRE LERYGINFG ATNYSAAYAT GFLIGRRALT AMSLDEVYRP KEIDGTYSVT KDIDGEKKAP RVFLDIGLAR STRGARVFGA L KGASDAGL NVPHSAAKFY GHKPDGSFDA KELHDRIFGY NISEYMKQLR DGDPEKYKTQ FSSYIKKGID PEEIPRIYKS AF DKIAEDP TRKKKETKDY SGFKKYKQAR LTYEERAKRV QAKLSAAEGE

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Macromolecule #31: 60S RIBOSOMAL PROTEIN L6

MacromoleculeName: 60S RIBOSOMAL PROTEIN L6 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 19.672766 KDa
SequenceString:
MLIKEVRVIP DDAGLYMPDD IPRFVEKYKA RTARKPRVRR TDLVKGMVVV VLEGVFASRR VVYLKGLEDN IALCAGPKSI NGIPLFRID ERYLLATSTV LDLNMDVDID GKDTVLTERD VYAVPMDVEM TDAERKIDEE IAKAARRVEY MKSYLSEPFE I DATRNFYS QKY

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Macromolecule #32: 60S ribosomal protein L7

MacromoleculeName: 60S ribosomal protein L7 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 27.987592 KDa
SequenceString: MEGVMSEAPQ SSIRKKEYEA RMSRIRSRQE KEFRERKSAN ARYAEETTEK LLNKYYEQER EILEKKSSMK NGFYVPKEAE FFAVILIRS KCNCPPKVRK VLELFRLKRI NTCVLVRNNK STRKMLQIIK DHVAFGTIGM ELLRKLVYTK GSGRNGHVRV K LTNEFIED ...String:
MEGVMSEAPQ SSIRKKEYEA RMSRIRSRQE KEFRERKSAN ARYAEETTEK LLNKYYEQER EILEKKSSMK NGFYVPKEAE FFAVILIRS KCNCPPKVRK VLELFRLKRI NTCVLVRNNK STRKMLQIIK DHVAFGTIGM ELLRKLVYTK GSGRNGHVRV K LTNEFIED MFDGKIRCIE ELVHHIYNGT EMFKKVNSFL YPFHLSPPRG GFKGQKSKSF NDGGSVGNHQ DLLSNLLERM I

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Macromolecule #33: 60S ribosomal protein L7a

MacromoleculeName: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 23.587896 KDa
SequenceString: MVLGKVKKIP VEEPRLTKEQ KIERDLLIKK KVSKLANSIR IPPAIYQFRT VLSEHDTKKF VELFMKYRPE NKQEKRIRLQ SEDPKKGPK PILVKFGLKH VTNLIETKKA KLVLISASVD PIEVVIFLPT LCRKMGVSYA IVENSTLLGK LVNLKTTSCV C LCDVRPED ...String:
MVLGKVKKIP VEEPRLTKEQ KIERDLLIKK KVSKLANSIR IPPAIYQFRT VLSEHDTKKF VELFMKYRPE NKQEKRIRLQ SEDPKKGPK PILVKFGLKH VTNLIETKKA KLVLISASVD PIEVVIFLPT LCRKMGVSYA IVENSTLLGK LVNLKTTSCV C LCDVRPED EGSFKEMLRT ADAIFLDNYE THLSTWGGLP QKEADEKQ

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Macromolecule #34: 60S RIBOSOMAL PROTEIN L9

MacromoleculeName: 60S RIBOSOMAL PROTEIN L9 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 20.649123 KDa
SequenceString:
MRILVTRIVK VPEDCTATQE GKIFTFQGPK GSIVEDCTRH PLTFDIHEGN IRIRLWNGKR KAMALAITVE SLLRNAIKAV TVGFAYTMK AVYNHFSINM EIKEDGKILL VKNFLGEKNV KRFRMRGAAR VRLDERKDTI VIEGPSLSDV SQSAGTITNE C KAKNRDSR VFLDGIFVTE RGIMA

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Macromolecule #35: 60S ribosomal protein L10

MacromoleculeName: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 25.047074 KDa
SequenceString: MGRRPGRCYR YLSKKAYPKS RFNRGVPDSK IQIFDLGRRK AGVLEFPLLV NCISNERENL SAEALEAARI CANKYMVKHA GKDNFHLRI RVYPFHVLRI NKMLSCAGAD RLQTGMRGSF GKPYGRAARV VFNQPILSIR TKESFRDAAV EALRRAKNKF P GHQKIQVS ...String:
MGRRPGRCYR YLSKKAYPKS RFNRGVPDSK IQIFDLGRRK AGVLEFPLLV NCISNERENL SAEALEAARI CANKYMVKHA GKDNFHLRI RVYPFHVLRI NKMLSCAGAD RLQTGMRGSF GKPYGRAARV VFNQPILSIR TKESFRDAAV EALRRAKNKF P GHQKIQVS SKFGFTNMFS DEFNKLNESG RIILRGGSFS VIREKGSIDK FKRDLEYAAN N

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Macromolecule #36: 60S ribosomal protein L11

MacromoleculeName: 60S ribosomal protein L11 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 19.733045 KDa
SequenceString:
MSSKKINPMR EISIKKLSIH ICARESGAKL DRAAKVLEQL TGQKPVTSKA RMTIRNFGIR RNEKIAVHVN VSGKKAYELL NTALRVKEY ELKESCFSNN GCFGFGIEEH IDLGLKYDPN IGIFGMDFFV ILARPGDRVS KRKRCKSRVG NKQRVYAEDA K KWFVENFE GVLVE

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Macromolecule #37: 60S RIBOSOMAL PROTEIN L13

MacromoleculeName: 60S RIBOSOMAL PROTEIN L13 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 18.900148 KDa
SequenceString:
MKGNHALPNN HFRKTSLKIR IHHDPETKAR VMAEKKLRKA KALFPMPLKK LRPIVRCPTI KYNRNERLGR GFTAAECEKA GLDYRHARR LGIAVDLRRR DTNQEAFDKN VERIKTYLGK ITIYESVKEA RESGAKPYTK EIMPFVKPKP VVGSISREEV A SYE

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Macromolecule #38: ECU06_1215 protein

MacromoleculeName: ECU06_1215 protein / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 12.275196 KDa
SequenceString:
MRTVRLGRIV TPALKERRHT YAIIVGIIDV TFVLLQRKDG EREICSVANL HLEDEAFDIK GLSAEEIGKL IPEDTYIEDT TNDFDRFKL KLRKRVEEEL LKEKGLA

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Macromolecule #39: Ribosomal protein L15

MacromoleculeName: Ribosomal protein L15 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 23.823924 KDa
SequenceString: MGAVNYLREI HRRKQSDVMR YLLRIRVCEY RQRGECFRVE KPTFLERART LGYKAKQGYV LYIARVKKGN MKRNYHNGNT RGKCVNAGI NQIKPSLRKQ ASAEMIAGKK CSNLGVLNSY WVGQDAAYKY YEVIMVDRDH QAIRNDPKIN WICKGTMKHR E CRGLTSAS ...String:
MGAVNYLREI HRRKQSDVMR YLLRIRVCEY RQRGECFRVE KPTFLERART LGYKAKQGYV LYIARVKKGN MKRNYHNGNT RGKCVNAGI NQIKPSLRKQ ASAEMIAGKK CSNLGVLNSY WVGQDAAYKY YEVIMVDRDH QAIRNDPKIN WICKGTMKHR E CRGLTSAS RKSRGLGKGV KFNKSKGGSI RSCWRRRNTL VLQKYR

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Macromolecule #40: 60S RIBOSOMAL PROTEIN L13A (L16)

MacromoleculeName: 60S RIBOSOMAL PROTEIN L13A (L16) / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 22.724789 KDa
SequenceString: MEKKEHVIID ATGQVAGKLA AKVAKLLLEG VRVTVVCAEE AVFVGSLERA MDKFKQYLNK RCLVNPRRGP FHFREPRMHL AKIIRRMIS YKKPRGKAAM SRLATYEGIP RELEGQPRYK VTCAFVKYTG NPNRYVTLGK LLSMFGWKHA EAAKSLTDEL K ERESLASL ...String:
MEKKEHVIID ATGQVAGKLA AKVAKLLLEG VRVTVVCAEE AVFVGSLERA MDKFKQYLNK RCLVNPRRGP FHFREPRMHL AKIIRRMIS YKKPRGKAAM SRLATYEGIP RELEGQPRYK VTCAFVKYTG NPNRYVTLGK LLSMFGWKHA EAAKSLTDEL K ERESLASL QKKDLDKKIE ELKTDKNFEN EVNRRLAMLA

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Macromolecule #41: 60S RIBOSOMAL PROTEIN L17

MacromoleculeName: 60S RIBOSOMAL PROTEIN L17 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 20.885357 KDa
SequenceString:
MGIKYSYEIH DPSKAVKAKI DNAKVSFKKT REASCTLRRR KVLDAIQYLK NVIAKTECVP FRRYNRGCGQ THQARKFSIP RREIVKANG EKKIKKGHVQ TRGRWPKKSA EFLISVLENI KENAETKGLD PKDLVITHVQ VSKAPKIFGR TFRAHGRVNP F NKSPCHIQ MVAEVLGSND VSIED

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Macromolecule #42: 60S RIBOSOMAL PROTEIN L18

MacromoleculeName: 60S RIBOSOMAL PROTEIN L18 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 22.655592 KDa
SequenceString: MYFTSAMKYT RLPPSATLSR KEPRSKNIYL RALSDFYTKV ASNSSVLAIK KISKRLKMSK SDRQPVKISK IVSEIGESKD KVAVVVAKV LDDDEVMVIP AVKVVALQWS KGVKEKIERY GGTIGTLDQL FELCPNMDDI CLVSTNKFSR RSAKFWGPAP G EKGSTTYP ...String:
MYFTSAMKYT RLPPSATLSR KEPRSKNIYL RALSDFYTKV ASNSSVLAIK KISKRLKMSK SDRQPVKISK IVSEIGESKD KVAVVVAKV LDDDEVMVIP AVKVVALQWS KGVKEKIERY GGTIGTLDQL FELCPNMDDI CLVSTNKFSR RSAKFWGPAP G EKGSTTYP RANQKCHNRE KRIMMRGRKS QHLKRQVVNK AT

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Macromolecule #43: 60S RIBOSOMAL PROTEIN L19

MacromoleculeName: 60S RIBOSOMAL PROTEIN L19 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 20.198928 KDa
SequenceString:
MSNEERVRRL ASDILNCGKK KLWLDNNEIE RLNGASTREQ IKQLIKDNVI IMKQDKHTSR GRCRKRLEAK KKGRHMGTGK RFGTANARM PQKKIWMKKI RAMRAMLKEM RANGEITKED FRVFYKQAKG HLFKHRFHMK DCIIKRKADE QRAKELAEQA K ALNLSNRV ISS

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Macromolecule #44: Uncharacterized protein ECU01_0250

MacromoleculeName: Uncharacterized protein ECU01_0250 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 19.453312 KDa
SequenceString:
MHFNIFVEGE FNNVKGVFID QSYPLRIQCT NCGSPHEKSV VLSEDSVGEG DFGEKVNLGI TCRCCRRIMT LKILKLKEGR EVKKHLLPT NFEDEFKEVW LSDMQKSRFL VSRIETNGAE VTSIESCILN LVSNQDVLFT NVNFEDRTVA KCNNLNMISS I IDFSLTVE LAK

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Macromolecule #45: ECU06_1135 protein

MacromoleculeName: ECU06_1135 protein / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 9.0199 KDa
SequenceString:
MSKTYLKSWK EKKEKMPNAA LSFKQRLRIK QQKRVERSAL LSKIKILKTR KRNFLRERQK QREMKKQENM AKS

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Macromolecule #46: 60S ribosomal protein L20

MacromoleculeName: 60S ribosomal protein L20 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 21.592184 KDa
SequenceString:
MKPETSRQAA FSKVNMTIPM TKEYRIHGSA MPTEDNPRPQ VFVGTIFTKN HVFAKAKFFK ILEKKYKIKA TKGLIIDCKE IPEPSFKEV QNYGIRFVYR SRSGVHNAYK ECRAISKCWA IDHVLRELAG RQKLKRSAVD IISVDVVPVE SLKRAKTIEF A DENVEFPI FTKIVNTKSL LIPSEYNPSD

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Macromolecule #47: 60S ribosomal protein L21

MacromoleculeName: 60S ribosomal protein L21 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 18.621852 KDa
SequenceString:
MRSKGYRRGT RYLFSQDHRK HGVAHVSKYL EKYEIGDMVD ILVNPAMMKG MPHKYYHGRT GRVYDVKPRS LNVALYKRVR GKYVIKKII VRIEHVRKSR CSEESQKRIL MAAEMARDAE SRGVVLAPSK RKIEGPRKAV EVSLDNNQPI EVGYEPHVVI F

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Macromolecule #48: 60S ribosomal protein L22

MacromoleculeName: 60S ribosomal protein L22 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 12.598611 KDa
SequenceString:
MTAASEKTTR RFTIDCTKPA SDSLISPSDL GAFLQQKIKC YTGKKEKLLH INANGNIVEV DVTGGFIGKQ GLKWQIGRFL HMKKLRAFI KIFAQGLDGF ELRYINVEEG KEE

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Macromolecule #49: 60S ribosomal protein L23

MacromoleculeName: 60S ribosomal protein L23 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 16.383464 KDa
SequenceString:
MAAEKKTEVL EKKISIKPRY KMTRGIQVET LMKCADNSGA KILRCIGVKR YRGRLNRLPA AAPGDICVVS VKKGKPELRK KVHYAILIR QKKIWRRTDG SHIMFEDNAA VLINNKGELR GAQIAGPVPR EVADMWPKIS SQASSIN

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Macromolecule #50: Similarity to 60S RIBOSOMAL PROTEIN L24

MacromoleculeName: Similarity to 60S RIBOSOMAL PROTEIN L24 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 11.350204 KDa
SequenceString:
MIQGVSMFSG RNIPKGSGIY EVMSDGRAVL TASNKERRLI ARKINPRDIR WTTSSREFFG KESQYVVEKM NVQKVKVVRG FRHISASSL ERAKEAGLPK K

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Macromolecule #51: 60S RIBOSOMAL PROTEIN L23A

MacromoleculeName: 60S RIBOSOMAL PROTEIN L23A / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 11.839871 KDa
SequenceString:
MESPRKNFKH PRNLVTKMPR NTPNDIILHA LTTEKAARCM EQFNTLVFYV EKTSTKPEIK KAITELYGSK VKKVNTLINK HGKKAYVSF ADEGAALEIA AKIGTI

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Macromolecule #52: 60S RIBOSOMAL PROTEIN L26

MacromoleculeName: 60S RIBOSOMAL PROTEIN L26 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 16.811482 KDa
SequenceString:
MKNLRKQKTS SRRKQRKAHF ACNDGEKRIR SSARLSRELR KEYGFRTFPL RTGDTVVVKG GKFDGKEGVI SKINYRDYKV YIEGCFVTK NDGTNALVPI YPSKLTITKF FLENGRKEQL ERKKRAREHG MEILRKRQEE VVSN

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Macromolecule #53: 60S RIBOSOMAL PROTEIN L27

MacromoleculeName: 60S RIBOSOMAL PROTEIN L27 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 14.508162 KDa
SequenceString:
MFFVPNMIVV VTSGKNAGKK AVVIKQIDER YVLTACVVRI PKEPEDHQPK WIKRRNAKFY IILKKYNIAH LIATRYKADL GLATIDYSG IEDNAETKKV LTNKTKDAMM KAWKENKAKF LFSSLSF

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Macromolecule #54: 60S ribosomal protein L27a

MacromoleculeName: 60S ribosomal protein L27a / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 17.066785 KDa
SequenceString:
MTDRVKKTRK LRGHVSHGYG RVGKHRKHSG GRGLAGGFSH MKTFFTRFHP DYHGKRGMRV YHRKENSDYA RPISSARLWG MIPKEQRYD FLDNPEKVPV IDVREFGYHV VVGGKLSLER PIVVKARYFT PSAKEEITKV GGKWIITY

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Macromolecule #55: 60S ribosomal protein L29

MacromoleculeName: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 6.565641 KDa
SequenceString:
MAKRKNHTNH NQNRKDHRGG IKKPKMPKFS EFLGLHPATL ARLEEAGKLW NAATSRK

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Macromolecule #56: 60S RIBOSOMAL PROTEIN L30

MacromoleculeName: 60S RIBOSOMAL PROTEIN L30 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 11.785833 KDa
SequenceString:
MRGKSNKGDN VTSSLLLAMR TGKYIVGFKR AIKSVIMKKA KCLVISSNFP STKRKLLEYY SVLAGGLPII FHSANNEELG SITERGHRL GCISIIDQGE AELIPAKGE

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Macromolecule #57: 60S ribosomal protein L31

MacromoleculeName: 60S ribosomal protein L31 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 13.035338 KDa
SequenceString:
MWSKIEENQT VEMTVNLRRI CSRLPWTKKA SKVVRMLKRE IQKHFREEIG VVVTNDLNNF LYSRGIKKIP NKVRVRVTKE TSLKNAEEN VLKTDLVVVG SFKNLKEVIV DQ

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Macromolecule #58: 60S ribosomal protein L32

MacromoleculeName: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 16.376375 KDa
SequenceString:
MSSELFDPKP LVEIKEAYRK NKKFIRHHSD RYKRVKPSWR RPHGIDSKVR KRCKGEREMP SIKYKKPKEI RHLLPNGLRK VRIFNINDL TPLTSLNRFY CGEIAHAVGA RKRIAIVNRA KELGICLLNG NARLIPEIEE

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Macromolecule #59: 60S RIBOSOMAL PROTEIN L35A (L33)

MacromoleculeName: 60S RIBOSOMAL PROTEIN L35A (L33) / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 12.832846 KDa
SequenceString:
MTGVMMNEEV LSRTTIPATF VSFKRGQRRL HPRHVLLKIR NVTSKDEASS YVGNGVVCYK KNNEGERYPV HGVITRIHGN SGAVRARFT RNLDPKLLGT HVFVKLYKVE ADEI

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Macromolecule #60: 60S ribosomal protein L34

MacromoleculeName: 60S ribosomal protein L34 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 12.463718 KDa
SequenceString:
MKNVLIHKGA TYKTRSNRRR KVRTPSGKLV NRRVKKHSKK HRCHECNAIL GSIARMRPAE FSRQKVSARR VNRPYGATTC GRCVREKII SAFLGNEERI VMEKTGAAAG G

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Macromolecule #61: 60S ribosomal protein L35-1

MacromoleculeName: 60S ribosomal protein L35-1 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 14.128682 KDa
SequenceString:
MKIEASALRQ LGIKQIEERA AEIKADLAAL RQKKNSGDVG ANDIKTAKKN LARALTVRRE KILEELVEAY RGTPVSKLPK ELRPKLNRS KRRALTKTQL RRKTRRQRAR MSKFPRVIFA YNE

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Macromolecule #62: 60S ribosomal protein L36

MacromoleculeName: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 10.776858 KDa
SequenceString:
MKNAYKKVRV RYPVKRPDVK RKQRGPRAET QESRFLAAAV ADEISGLSPL EKKAISLLEA KNNNKAQKLL RKRLGSHKRA VAKVEKLAR MLLEK

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Macromolecule #63: 60S ribosomal protein L37

MacromoleculeName: 60S ribosomal protein L37 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 10.416252 KDa
SequenceString:
MSKGTASFGK KNKRNTEMCR RCGRQSYHKQ KNSCSSCGYP NPKMRNPGSI KARRRRTIGT GRMRYMKREL RAAKNGHKGD PILRTLWAK N

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Macromolecule #64: 60S ribosomal protein L39

MacromoleculeName: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 6.517931 KDa
SequenceString:
MGSRKTALVK TRLMRALKRN REIPAWKRMM KEHKGEYNRA RRHWRSKKLK IY

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Macromolecule #65: UBIQUITIN/ L40 RIBOSOMAL PROTEIN FUSION

MacromoleculeName: UBIQUITIN/ L40 RIBOSOMAL PROTEIN FUSION / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 14.312952 KDa
SequenceString:
MQIGVRFCGK TSFVQAEPSQ SVLSLKALVG RACGLDSSVM VLQHNSRILE DSGTMAGYGM RTLDTITAYP KLLGGGGNMS DNDAAMAMK EKNDCLICRR CYARSGKAAE KCRKCFSKDL RLKKPLKAMK KK

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Macromolecule #66: 60S ribosomal protein L44

MacromoleculeName: 60S ribosomal protein L44 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 12.035392 KDa
SequenceString:
MVNIPKTRNT FCKKCKGYSA HKVSQAKKSK DNPRAQGNRR YARKQRGYGG QTKPILRRKA KVTKKLVLNL ECTKCKFSHQ KPLKRAKHV IFGGERKRKG EALVY

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Macromolecule #67: 60S RIBOSOMAL PROTEIN L37A (L43)

MacromoleculeName: 60S RIBOSOMAL PROTEIN L37A (L43) / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 9.849786 KDa
SequenceString:
MGKGTKKVGI AGSFGARYGS TLRKRMKAVL ETQRAKYICE ACGKKTVKRE AVGIWKCRSC RRVFAGGSYA KTTPAGKIFI NMVKQYNTK

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Macromolecule #68: 40S ribosomal protein S0

MacromoleculeName: 40S ribosomal protein S0 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 27.899887 KDa
SequenceString: MPQDNTRISD SIKIPDEFVK LLIVSQSHLG GTSTNKSFAR YLYGTRPRDR INIIDINATW EKLIIAARAF CGIKHPSSIA VVSTKTFGR KPVVKFCEAV GATPITGRFI PGSFTNSEVK RVYDPRVLIV SDTYADKQAI LESQYCNLPT IAFVNTDNSL V GVDIAIPM ...String:
MPQDNTRISD SIKIPDEFVK LLIVSQSHLG GTSTNKSFAR YLYGTRPRDR INIIDINATW EKLIIAARAF CGIKHPSSIA VVSTKTFGR KPVVKFCEAV GATPITGRFI PGSFTNSEVK RVYDPRVLIV SDTYADKQAI LESQYCNLPT IAFVNTDNSL V GVDIAIPM NNRSPSAIAA GFFILSRLIN YMKTGAELVR DMKEVELFLF RDSVELEQLV EEQLLETTDS ILNVGKEGIL SG IGTGNAD EWNSF

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Macromolecule #69: 40S ribosomal protein S1

MacromoleculeName: 40S ribosomal protein S1 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 26.98535 KDa
SequenceString: MAIQPPGSYP QGNKKGKAKK KSGQNAFARK EWYTLRSPSI FPNNINGKTL MTKSAGKNSY MNMIGRRYDV NQADLTGNND VGHRKFIFK IGDIKGSECV GFFDGMELTS DKHKAMIKKW HTLIEAQKDI VAKDGSVFRV FVMGVTRRHP GYVKKTCYVK H SDEKKIRK ...String:
MAIQPPGSYP QGNKKGKAKK KSGQNAFARK EWYTLRSPSI FPNNINGKTL MTKSAGKNSY MNMIGRRYDV NQADLTGNND VGHRKFIFK IGDIKGSECV GFFDGMELTS DKHKAMIKKW HTLIEAQKDI VAKDGSVFRV FVMGVTRRHP GYVKKTCYVK H SDEKKIRK IMFDVIEEEL SGCDVQKIMK KLANETVGKR IEELGSEIFP LQNCCVRKVK TIKSHQTASV GQQEAIDAIT N

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Macromolecule #70: 40S RIBOSOMAL PROTEIN S2

MacromoleculeName: 40S RIBOSOMAL PROTEIN S2 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 26.270578 KDa
SequenceString: MEGTERRNPG KPFPRKEESE WIPKTAVGRL VKMLKITSLE QIFKFSHVIR EPEIVDLLCG NSIKEEVLSI KSVQKQTRAG QKTRIKAVV VVGDRKGHVG IGTKASKEAA SAIRGAIARA KCAIRPVRLG LWDGAQGKPH TVSCKGSGKC GTSVVQVIPA P RGTGIISG ...String:
MEGTERRNPG KPFPRKEESE WIPKTAVGRL VKMLKITSLE QIFKFSHVIR EPEIVDLLCG NSIKEEVLSI KSVQKQTRAG QKTRIKAVV VVGDRKGHVG IGTKASKEAA SAIRGAIARA KCAIRPVRLG LWDGAQGKPH TVSCKGSGKC GTSVVQVIPA P RGTGIISG SVNKKIFELA GIKDLYTLSF GATSTTENFA KASIAALEAT SSMILPDQWE EKPRTLNPLL EFADILNQLD KS RV

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Macromolecule #71: 40S ribosomal protein S3

MacromoleculeName: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 24.318387 KDa
SequenceString: MSSDQVLERF MKNGLMNAEL KEFFEKALVN EGFSTMELRM QETPIKIILK VAKPHEAIGE KKFRLRQFQH LAAQRLEVPD ESVEIVVEK VHEKGLCALI QANFIREKIL GGVQYRRAVN MALKTARHAK AQGCQIIVSG KLKGQRAKSV KFQDGVLIHS G DAVKDYIN ...String:
MSSDQVLERF MKNGLMNAEL KEFFEKALVN EGFSTMELRM QETPIKIILK VAKPHEAIGE KKFRLRQFQH LAAQRLEVPD ESVEIVVEK VHEKGLCALI QANFIREKIL GGVQYRRAVN MALKTARHAK AQGCQIIVSG KLKGQRAKSV KFQDGVLIHS G DAVKDYIN TGYATVETKQ GVIGIQVRIM LPYDPEGVLG PNYPLPDRIT ILEPSEIQ

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Macromolecule #72: 40S ribosomal protein S4

MacromoleculeName: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 30.090691 KDa
SequenceString: MPTGHRDHLK RKTAPKSWML DKQGGTFALM PASGPHKKTE CIPLGYLISR FLRYASNFKE LSIILAEKNI KVNGRVRTNS HFPVGLFDV VSIEKTGEHF RVLYNVHKKF HLHRISSEEA SYRLTKVTKK YENQGIPYIV SSCGLSIRFC DPAIDLGYTI K ISNETSKV ...String:
MPTGHRDHLK RKTAPKSWML DKQGGTFALM PASGPHKKTE CIPLGYLISR FLRYASNFKE LSIILAEKNI KVNGRVRTNS HFPVGLFDV VSIEKTGEHF RVLYNVHKKF HLHRISSEEA SYRLTKVTKK YENQGIPYIV SSCGLSIRFC DPAIDLGYTI K ISNETSKV IEYIVPGPDK VIFVSRGKAR GRVGVINTIS TQGKETVYGM TDFAGNTFSC TSKSCILIGE SSENIWITLP KE RGIRQSE LEKINAQLGE MIDTEVAEVS E

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Macromolecule #73: 40S ribosomal protein S5

MacromoleculeName: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 20.987223 KDa
SequenceString:
MAEINLFEKY SYEEVKVNNV SLRPYINLSR RGIVPHAATT ITKGTTGKAR IPIAERFVCS LMRHGRNSGK KRLAINIFED ACFIIHSMT KKNPLQVLVD AIVNSGPRED TARIGRAGSM RRTSVDVSPL KRISIAISNL SAGIRNASFR NRITLAEAIA N ELIAASTN SQNSYAVNKK KEIERIAQSN R

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Macromolecule #74: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 25.518744 KDa
SequenceString: MKLNIAYPTN GTQKMFEIER RNEVRLYDKK IGDQFDGGIL GEEFEGTIME ITGGDDYQGF PMVSGHLTKK RVRPLLSKGD AGYRCRRKG VRRRKSVRGS IVSEEICVLN LIILRPGEKE IDGLTNAVND VSHLPRKEKK LRKMFGVPDE EKNVVGYIRN I LKSECDDP ...String:
MKLNIAYPTN GTQKMFEIER RNEVRLYDKK IGDQFDGGIL GEEFEGTIME ITGGDDYQGF PMVSGHLTKK RVRPLLSKGD AGYRCRRKG VRRRKSVRGS IVSEEICVLN LIILRPGEKE IDGLTNAVND VSHLPRKEKK LRKMFGVPDE EKNVVGYIRN I LKSECDDP KKIPKIRHNG KRMKREQERR EQIMKIRLER KRILEEERKE YLEKYFNKA

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Macromolecule #75: 40S ribosomal protein S7

MacromoleculeName: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 19.032094 KDa
SequenceString:
MSTQTEAEMS IAGIIRKHAK DISAPPEVFD KISIQIIICD GGEKIMVVKV PRSILHGVQM NYSNIIKAAK QQFHDYYIMF VRNFEAEGG GKTMTKRKAK EVEEVWLANA CFPFLLTGTR TDVRGVDDMV VNVLLERRTS LSRAEMDAIG AALHGLLGKN Y IVDVNHHT KN

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Macromolecule #76: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 19.615764 KDa
SequenceString:
MGINHRGDHK RRKTGAKAVI SRKKRNNRAG SQPSSTKIGE RKVTPVRVRG GNRKYKALRL DMGHFKFITT GKFRMAKLLQ VVYHPSSNE LVRTNTLTKS SVVKISAEPF KNDIKDVARD VDPSLHESFE KGHLYAIITS RPGQVGMAQG HVLQGDELKF Y ADKFNKKS KEVRK

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Macromolecule #77: 40S ribosomal protein S9

MacromoleculeName: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 20.954518 KDa
SequenceString:
MAGKKGSKRA STPRDPFEKE RLIREAQLVG VYGLKNKHEL WVMEKIFAKD KERARILLTS TNPEDIPING RSLLRRLMKY GILGGIDLC DKQEVVNGLN KVLDLTINHY LERRLQFRVF AAGLAKSVHH ARLLIGGRCI SIKDQIVDVP GFMVRADKEP L IEYNPYSR YGDKGKKKAE AKVGEE

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Macromolecule #2: 5.8S-23S ribosomal RNA

MacromoleculeName: 5.8S-23S ribosomal RNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 807.085 KDa
SequenceString: GUUUCUCUGU UCACGGGAUU GAUUGGCAUU AGCGGCGAUG AAGGACGUGC AGGAGGACGA UAUGCGUUGU UGUUGUGACG GUGUCUGAA UUGUGUGCGG UGUGCACGGG GACCCCUUGG ACUUAAGCAU AUCAGUAAAG GGAGGAGAAG AAACCAAAUG G GAUUGCCU ...String:
GUUUCUCUGU UCACGGGAUU GAUUGGCAUU AGCGGCGAUG AAGGACGUGC AGGAGGACGA UAUGCGUUGU UGUUGUGACG GUGUCUGAA UUGUGUGCGG UGUGCACGGG GACCCCUUGG ACUUAAGCAU AUCAGUAAAG GGAGGAGAAG AAACCAAAUG G GAUUGCCU GAGUAGCGGC GAGUGAAAUG GCAUGAGCCU GUGUGGUAAC UGCGUUUGGC GGGUUGUGUU UGUGGCAGCA GC GGCGAGU GCGAUUGAAU UCGGAUGCGG AGAGGGCGAU GUGCCUGUAG GCGCGGCGGC UUUUUUUGUG AGUAGUGUUG CUU GGUAGU GCAGCGCGAA UGCGGUGGUG GAUGCACCGA AGGCUAAAUA UGGCUGAUGA CCGAUAGCGG AUAAGUAGAG UGAU UGAAG UGUGAAAAGG AGGAUGAUGA GAAAUGAAAG UGAAUGGAGU GAAUGGGGGA UGUUCCCGCC CGUCUUGAAA CACGG ACCA AGGAGACGAC GGCAGGAUUG UUUUCCGAGA GGGGAUGGAU GCUGCGGUCG GACCCGAAAG AUGGUGAGCU AUGUGU GUG UUGAUUGAAG GGAUGCGAAA GUGUUCUGGA GGAUCUAAGC CGUUCUGACG UGCAACUCGA UGGCAUGACA UGUGCAU AG GGGCGAAAGA CCAAACGAAC CAUCUGGUAG CUGGUUCCCU CUGAAAUGUC UCUCAGGACA GCGGCGACGG GGAGUGUA A GAUGUUGUAA AGCACUAAUG CGCUUGCGUU AAACUUUGAA GAUGUCUUUU AAGUGUGUUG CCAGUGGGCA UACUGUGGU AAGCAUGGUG UGCGAGAAGG GAUGAACCUU UAAGCGGGCU AAGGAGUCUU AUGCAAGGUG AGUGAAUCCG GCGGCCUGAU UUUGACAGU UGGACGGUGG CCAUGGAGGU UGGCAUCCGC UAAGAAACGU GUUGCAACGU ACCUACCGAA UUGGGCGGCG G AGAAAAUG GAAGGGAUUG AAUUGCAUUC CGAUGCCUGC GGGGGAGUAU CUGAGUAAGG GGGCGGUAUC GUUCUGCUGA AG GUCCUGC GUGAGCGGGG GUGGAGGGGC GAUAUGUGCA GAUCUUGGUA GCAGUAACAA GGAAAUGAUU UGCGCUGUGG ACG AGGGUU CCCUGUGUGG CAGGGGUGAA UCGACUGUGG AUACGGAGGU GCAUUGAUAG UUGUGUGUAU UGCGGCUGGG AGGC GACUU GGCUGUGACG GAUGGGGGCG GCCCUGUCCA GGAUAGCAGA ACUGGAUGCU GUCUGCUGCG GAUUGUUAUG AUGCG AAGC AGCGAUUGUG GGCGAGCCUG UUCCGAUGAA AAAGAGGCUG CUUUGUCGUA CCUAGACCGC AUCUGGUGUC CACUGU UUG CCGCGGAAGU GUUUUGUGCA AGGGCCGUAA GGGAAGUCGG CAAGAUUGAU GUGUAACUUC GGGAGAAGGA UUGGCUC UA GCGUGCUAGA ACUGUUUGUU GUGGGGAAUC UGACUGUUUA CUAAAAACAU AGCUUUGUGU GUGGACGCAA AGUGAAUU C UGCCCAGUGC ACUUUUAGUU ACGGCCUGUG UGGGCUAAGC GAGUGUAAAC GGCGGGAGUA ACUAUGACUC UCUUAAGGU AGCCAAAUGC CUCGUCAUGU AAUUAGUGAC GCGCAUGAAU GGAGCAACGA GAUUCCCACU GUCCCUACGG CCUGUCUUGU GAACCUACA GCCAAGGGAA CGGGCUUGGG CUGAUCAGCG GGGAAAGAAG ACCCUGUUGA GCUUGACUCU AGCAUGUGUG A AGUGCUGG GUGUGGGCAU GCAGCGAGGU GGGGAUGAGU GCGAUACCAC UAUGUCUUCC GCCCAGUGCU UUGUGGAUGG AU GUGGGGA GUUUGGCUGG GGCGGCACGA CUGCUAAAGA CUAACGCAGA CGUCCUAAGG CGGGCUCAGC GGGGAAGGAA ACC ACGCGU GGAGCACAAG GGCACAAGCC UGCUUGAGAC UGUUCCUAUU GCGGGACUGU UUGGGGGAAA CCCUGGCCUA GAGA UCCCA UGUCCUAUUG UGCGUGGGAC AUGGGUGACA GAAAAGUUAC CACAGGGAUA ACUGGCUUGU AGCAGCCGAG CGACC AUAG CGACGCUGCU UUUUGAUUCU UCGAUGUCGG CUCUUCCUAU CAUCGCGAAG CAGAGAUUGC GAAGUGUUGG AUUGUU CAC CCACCAACAG GGAACGUGAG CUGGGUUUAG ACCGUCGUGA GACAGGUUAG UUUUACCCUA CUGUGUGGAC UGUGCAG CA GAUCUUGAUA GUACGAGAGG AACUCUUGAU UGCGACCUCU GGUGGAGCGG CGUGCCUGCU GGCUGCUGCG GAGCUACG U CGGGAGUAUA AUGGCUGAAG GCCUCUAAGC CUGAAGACCG ACUGCGCGGU GUGUGUGGCG GAAGACGACC GCUGUGUGU GCAAGCUGUG CGGAUGGAGG AGGAUUCCGA CAUUUUGAGG CUGUGCAUUU GCGCGAGUGU GUGUGGAGAG CUCUUAUUGC ACUGUG

+
Macromolecule #3: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 38.478938 KDa
SequenceString:
AGUUGCGGCC AUAUCCACGG AAAAGCACCG GAACUCGUCA GAUCUCCGAA GUUAAGCCUG UGCGAGCCUG GGUAGUACUG AGAAGGGAG ACCACUCGGG AAUACCGGGU GCUGCAACUU

+
Macromolecule #4: 18S ribosomal RNA

MacromoleculeName: 18S ribosomal RNA / type: rna / ID: 4 / Number of copies: 1
Source (natural)Organism: Encephalitozoon cuniculi GB-M1 (fungus) / Strain: GB-M1
Molecular weightTheoretical: 422.123406 KDa
SequenceString: CAUCAGGUUG AUUCUGCCUG ACGUGGAUGC UAUUCUCUGG GGCUAAGCCA UGCAUGCUUG UGAACUCUUU GUGGGGGAUU AGCGGACGG CUCAGUGAUA GCACGAUGAU UUGUUUGCGG GAUGAGCAGU AGCUGCGGGA AACUGCAGAU AGUGGUCUGC C CCUGUGGG ...String:
CAUCAGGUUG AUUCUGCCUG ACGUGGAUGC UAUUCUCUGG GGCUAAGCCA UGCAUGCUUG UGAACUCUUU GUGGGGGAUU AGCGGACGG CUCAGUGAUA GCACGAUGAU UUGUUUGCGG GAUGAGCAGU AGCUGCGGGA AACUGCAGAU AGUGGUCUGC C CCUGUGGG GGUUGGCAAG UAAGUUGUGG GCCUAUCAGC UGGUAGUUAG GGUAAUGGCC UAACUAGGCG CAGACGGGAU AC GGGGGAU CAGGGUUUGG UUCCGGAGAG GGAGCCUGAG AGAUGGCUAC UACGUCCAAG GAUGGCAGCA GGCGCGAAAC UUG CCUAAU CCUUUGGGGA GGCGGUUAUG AGAAGUGAUG UGUGUGCGAG UGCAAAGGGG UCGCAUGUGA UUGGAGGGCA AGUC GGGUG CCAGCAGCCG CGGUAAUACC UGCUCCAAUA GUGUCUAUGG UGGAUGCUGC AGUUAAAAUG UCCGUAGUCU GUUGU GUAU GUCUUUGUGU GUGAUGUUUG UGGUUGUGUG UGGAUGUAGU GAUGUGUGUG GCAGAGGACG AGGGGCACUG GAUAGU UGG GCGAGAGGUG AAAUGCGAAG ACCCUGACUG GACGAGCGGA AGCGAAGGCU GUGCUCUUGG ACUAAUGUUG CGAUGAA GG ACGAAGGCUA GAGGAUCGAA AUCGAUUAGA UACCGUUUUA GUUCUAGCAG UAAACGAUGC CGACUGGACG GGACAGUG U GUGUUGUCCA UGAGAAAUCU UGAGUAUGCG GGUUCUGGGG AUAGUAUGCU CGCAAGAGUG AAACUUGAAG AGAUUGACG GAAGGACACC ACAAGGAGUG GAGUGUGCGG CUUAAUUUGA CUCAACGCGG GGCAACUUAC CGGCUCUGAA GGAUGCCUGU GAGUGCAUG GCAUGAGGCA UGCGGCGGUG GUGCAUGGCC GUUUUAAAUG GAUGGCGUGA GCUUUGUCUU AAGUUGCGUA A GAUGUGAG ACCCUUUGAC GGUGUUCUAC GGAGCAAGGA GGGGAUGGAA GAGAACAGGU CCGUUAUGCC CUGAGAUGAG GC GGGCUGC ACGCGCACUA CGAUAGAUGG CGCUUCUGCC UGCUGUGAGG GAUGAAGCUG UGUAAGGGGC UUCUGAACGU GGA AUUCCU AGUAAUAGCG GCUGACGAAG CUGCUUUGAA UGUGUCCCUG UCCUUUGUAC ACACCGCCCG UCGCUAUCUA AGAU GACGC ACUGGACGAA GAUCGGAAGG UCUGAGUCCU GAGUGUUAGA UAAGAUAUAA GUCGUAACAU GGCUGCUGUU GGAGA ACCA GCAGCAGGAU CAGUAU

+
Macromolecule #78: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 78 / Number of copies: 8 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #79: ADENOSINE MONOPHOSPHATE

MacromoleculeName: ADENOSINE MONOPHOSPHATE / type: ligand / ID: 79 / Number of copies: 1 / Formula: AMP
Molecular weightTheoretical: 347.221 Da
Chemical component information

ChemComp-AMP:
ADENOSINE MONOPHOSPHATE / AMP*YM

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Macromolecule #80: SPERMIDINE

MacromoleculeName: SPERMIDINE / type: ligand / ID: 80 / Number of copies: 1 / Formula: SPD
Molecular weightTheoretical: 145.246 Da
Chemical component information

ChemComp-SPD:
SPERMIDINE

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
Component:
ConcentrationNameFormula
50.0 mMHEPES-KOH
25.0 mMmagnesium chlorideMgCl2
130.0 mMpotassium chlorideKCl
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 400 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR / Details: Quorum GloQube, 10 mA
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV
Details: Quantifoil grids (R1.2/1.3, 400 mesh, copper) were glow discharged (10 mA, 30s, Quorum GloQube), and 3 microlitres of the crude sample of E. cuniculi ribosomes (300 nM) was pipetted onto a ...Details: Quantifoil grids (R1.2/1.3, 400 mesh, copper) were glow discharged (10 mA, 30s, Quorum GloQube), and 3 microlitres of the crude sample of E. cuniculi ribosomes (300 nM) was pipetted onto a grid. Excess sample was immediately blotted off and vitrification was performed by plunging the grid into liquid nitrogen-cooled liquid ethane at 100% humidity and 4 degrees celsius using an FEI Vitrobot Mark IV (Thermo Fisher).

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number real images: 2210 / Average exposure time: 1.35 sec. / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.6 µm / Nominal defocus min: 0.8 µm / Nominal magnification: 96000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

DetailsDrift-corrected and dose-corrected averages of each movie were created using RELION 3.1s own implementation of motion correction and the contrast transfer functions estimated using CTFFIND-4.1
Particle selectionNumber selected: 260895
Details: Particles were picked using Laplacian-of-Gaussian autopicking and reference-free 2D classification used to generate templates for further autopicking. The resulting particles were extracted ...Details: Particles were picked using Laplacian-of-Gaussian autopicking and reference-free 2D classification used to generate templates for further autopicking. The resulting particles were extracted with binning-by-4, and 2D and 3D classification performed to remove junk images
CTF correctionSoftware: (Name: RELION (ver. 3.1), CTFFIND (ver. 4.1))
Startup modelType of model: INSILICO MODEL
In silico model: The 3D classification step was carried out using a 60 Angstroms low-passed filtered ab initio starting model made using a stochastic gradient descent procedure.
Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.7 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1)
Details: The remaining particles were re-extracted without binning and aligned and refined in 3D, again using a 60 Angstrom low-passed filtered ab initio starting model. Rounds of CTF refinement and ...Details: The remaining particles were re-extracted without binning and aligned and refined in 3D, again using a 60 Angstrom low-passed filtered ab initio starting model. Rounds of CTF refinement and Bayesian polishing were performed until the map resolution stopped improving. 108,005 particles fed into the final 3D reconstruction of estimated resolution 2.7 Angstrom.
Number images used: 108005
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final 3D classificationNumber classes: 4 / Avg.num./class: 40493 / Software - Name: RELION (ver. 3.1)
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial model(PDB ID:

4v88

,

6rm3

)
DetailsThe model was built using fragments of S. cerevisiae (pdb id 4v88) and V. necatrix ribosomes (pdb id 6rm3) as starting models that were edited using Coot using genomic sequences of the E. cuniculi strain GB-M1 to model rRNA and ribosomal proteins. For ribosomal proteins that are encoded by two alternative genes (with one gene coding for a zinc-coordinating protein and another gene coding for a zinc-free ribosomal protein), we used zinc-coordinating isoforms, because the cryo-EM map revealed the presence of these isoforms and not their zinc-free paralogs in the ribosome structure. The identity of protein msL2 in the ribosome structure was determined using the genomic sequence of the E. cuniculi strain GB-M1 and the cryo-EM map that revealed a unique combination of aromatic and bulky amino acids in its structure: the cryo-EM map showed that msL2 has a tyrosine residue at position 5, a tryptophan residue at position 9, and lysine or arginine residues at positions 10, 12 and 13. The only protein with this sequence was the hypothetical protein ECU06_1135, whose sequence and length were fully consistent with the cryo-EM map. The structure of E. cuniculi ribosomes was refined using Phenix real space refine and validated using MolProbity within Phenix and PDB OneDep. The parts of the model corresponding to the 60S, 40S body and 40S head were built and refined using the consensus map, 40S body multibody map and 40S head multibody map, respectively.
RefinementSpace: REAL / Protocol: OTHER / Target criteria: correlation coefficient
Output model

PDB-7qep:
Cryo-EM structure of the ribosome from Encephalitozoon cuniculi

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