Journal: Nature / Year: 2022 Title: Structure of pathological TDP-43 filaments from ALS with FTLD. Authors: Diana Arseni / Masato Hasegawa / Alexey G Murzin / Fuyuki Kametani / Makoto Arai / Mari Yoshida / Benjamin Ryskeldi-Falcon / Abstract: The abnormal aggregation of TAR DNA-binding protein 43 kDa (TDP-43) in neurons and glia is the defining pathological hallmark of the neurodegenerative disease amyotrophic lateral sclerosis (ALS) ...The abnormal aggregation of TAR DNA-binding protein 43 kDa (TDP-43) in neurons and glia is the defining pathological hallmark of the neurodegenerative disease amyotrophic lateral sclerosis (ALS) and multiple forms of frontotemporal lobar degeneration (FTLD). It is also common in other diseases, including Alzheimer's and Parkinson's. No disease-modifying therapies exist for these conditions and early diagnosis is not possible. The structures of pathological TDP-43 aggregates are unknown. Here we used cryo-electron microscopy to determine the structures of aggregated TDP-43 in the frontal and motor cortices of an individual who had ALS with FTLD and from the frontal cortex of a second individual with the same diagnosis. An identical amyloid-like filament structure comprising a single protofilament was found in both brain regions and individuals. The ordered filament core spans residues 282-360 in the TDP-43 low-complexity domain and adopts a previously undescribed double-spiral-shaped fold, which shows no similarity to those of TDP-43 filaments formed in vitro. An abundance of glycine and neutral polar residues facilitates numerous turns and restricts β-strand length, which results in an absence of β-sheet stacking that is associated with cross-β amyloid structure. An uneven distribution of residues gives rise to structurally and chemically distinct surfaces that face external densities and suggest possible ligand-binding sites. This work enhances our understanding of the molecular pathogenesis of ALS and FTLD and informs the development of diagnostic and therapeutic agents that target aggregated TDP-43.
History
Deposition
Oct 8, 2021
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Header (metadata) release
Dec 15, 2021
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Map release
Dec 15, 2021
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Update
Jan 19, 2022
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Current status
Jan 19, 2022
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
EMPIAR-10831 (Title: Structure of pathological TDP-43 filaments from ALS with FTLD (Individual 1, motor cortex) Data size: 2.0 TB Data #1: Unaligned multiframe movies [micrographs - multiframe]) EMPIAR-10832 (Title: Structure of pathological TDP-43 filaments from ALS with FTLD (Individual 2, frontal cortex) Data size: 2.9 TB Data #1: Unaligned multiframe movies [micrographs - multiframe])
Entire : Pathological TDP-43 filaments extracted from the motor cortex of ...
Entire
Name: Pathological TDP-43 filaments extracted from the motor cortex of an individual that succumbed to ALS with FTLD.
Components
Tissue: Pathological TDP-43 filaments extracted from the motor cortex of an individual that succumbed to ALS with FTLD.
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Supramolecule #1: Pathological TDP-43 filaments extracted from the motor cortex of ...
Supramolecule
Name: Pathological TDP-43 filaments extracted from the motor cortex of an individual that succumbed to ALS with FTLD. type: tissue / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)
Organism: Homo sapiens (human) / Tissue: Brain
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Experimental details
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Structure determination
Method
cryo EM
Processing
helical reconstruction
Aggregation state
filament
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Sample preparation
Buffer
pH: 7.4
Vitrification
Cryogen name: ETHANE
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Electron microscopy
Microscope
FEI TITAN KRIOS
Image recording
Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 39.7 e/Å2
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron optics
Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
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