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- EMDB-13588: Gelsolin-free CCT -

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Basic information

Entry
Database: EMDB / ID: EMD-13588
TitleGelsolin-free CCT
Map data
Sample
  • Complex: Gelsolin-free CCT
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.5 Å
AuthorsCuellar J / Vallin J / Svanstrom A / Maestro-Lopez M / Bueno-Carrasco MT / Ludlam WG / Willardson BM / Valpuesta JM / Grantham J
Funding support Spain, 1 items
OrganizationGrant numberCountry
Other governmentPID2019-105872GB-I00/AEI/10.13039/501100011033 Spain
CitationJournal: J Mol Biol / Year: 2022
Title: The Molecular Chaperone CCT Sequesters Gelsolin and Protects it from Cleavage by Caspase-3.
Authors: Jorge Cuéllar / Josefine Vallin / Andreas Svanström / Moisés Maestro-López / María Teresa Bueno-Carrasco / W Grant Ludlam / Barry M Willardson / José M Valpuesta / Julie Grantham /
Abstract: The actin filament severing and capping protein gelsolin plays an important role in modulation of actin filament dynamics by influencing the number of actin filament ends. During apoptosis, gelsolin ...The actin filament severing and capping protein gelsolin plays an important role in modulation of actin filament dynamics by influencing the number of actin filament ends. During apoptosis, gelsolin becomes constitutively active due to cleavage by caspase-3. In non-apoptotic cells gelsolin is activated by the binding of Ca. This activated form of gelsolin binds to, but is not a folding substrate of the molecular chaperone CCT/TRiC. Here we demonstrate that in vitro, gelsolin is protected from cleavage by caspase-3 in the presence of CCT. Cryoelectron microscopy and single particle 3D reconstruction of the CCT:gelsolin complex reveals that gelsolin is located in the interior of the chaperonin cavity, with a placement distinct from that of the obligate CCT folding substrates actin and tubulin. In cultured mouse melanoma B16F1 cells, gelsolin co-localises with CCT upon stimulation of actin dynamics at peripheral regions during lamellipodia formation. These data indicate that localised sequestration of gelsolin by CCT may provide spatial control of actin filament dynamics.
History
DepositionSep 16, 2021-
Header (metadata) releaseApr 27, 2022-
Map releaseApr 27, 2022-
UpdateApr 27, 2022-
Current statusApr 27, 2022Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_13588.png

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Map

FileDownload / File: emd_13588.map.gz / Format: CCP4 / Size: 30.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

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AxesZ (Sec.)Y (Row.)X (Col.)
1.37 Å/pix.
x 200 pix.
= 274. Å		1.37 Å/pix.
x 200 pix.
= 274. Å		1.37 Å/pix.
x 200 pix.
= 274. Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.37 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0057
Minimum - Maximum-0.014934688 - 0.040040158
Average (Standard dev.)-2.3540357e-05 (±0.0025643415)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions200200200
Spacing200200200
CellA=B=C: 274.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_13588_half_map_1.map
Projections & Slices
AxesZYX

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Half map: #1

Fileemd_13588_half_map_2.map
Projections & Slices
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Sample components

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Entire : Gelsolin-free CCT

EntireName: Gelsolin-free CCT
Components
  • Complex: Gelsolin-free CCT

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Supramolecule #1: Gelsolin-free CCT

SupramoleculeName: Gelsolin-free CCT / type: complex / ID: 1 / Parent: 0 / Details: Apo-CCT
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Homo sapiens (human) / Recombinant strain: (HEK)-293T

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TALOS ARCTICA
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 1.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

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Image processing

Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 42261
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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