- EMDB-13056: Tomogram of a nuclease treated shVim HO H222P MEF nuclear lamina -
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Database: EMDB / ID: EMD-13056
Title
Tomogram of a nuclease treated shVim HO H222P MEF nuclear lamina
Map data
A tomogram of nuclease treated shvim HO H222P MEF nucleus. The tomogram is dose-weighted, CTF-corrected and reconstructed in IMOD. Fudicial markers are replaced by noise.
Journal: J Cell Sci / Year: 2021 Title: A lamin A/C variant causing striated muscle disease provides insights into filament organization. Authors: Rafael Kronenberg-Tenga / Meltem Tatli / Matthias Eibauer / Wei Wu / Ji-Yeon Shin / Gisèle Bonne / Howard J Worman / Ohad Medalia / Abstract: The gene encodes the A-type lamins, which polymerize into ∼3.5-nm-thick filaments and, together with B-type lamins and associated proteins, form the nuclear lamina. Mutations in cause a wide ...The gene encodes the A-type lamins, which polymerize into ∼3.5-nm-thick filaments and, together with B-type lamins and associated proteins, form the nuclear lamina. Mutations in cause a wide variety of pathologies. In this study, we analyzed the nuclear lamina of embryonic fibroblasts from mice, which develop cardiomyopathy and muscular dystrophy. Although the organization of the lamina appeared unaltered, there were changes in chromatin and B-type lamin expression. An increase in nuclear size and consequently a relative reduction in heterochromatin near the lamina allowed for a higher resolution structural analysis of lamin filaments using cryo-electron tomography. This was most apparent when visualizing lamin filaments and using a nuclear extraction protocol. Averaging of individual segments of filaments in mouse fibroblasts resolved two polymers that constitute the mature filaments. Our findings provide better views of the organization of lamin filaments and the effect of a striated muscle disease-causing mutation on nuclear structure.
EMPIAR-10601 (Title: A lamin A/C variant causing striated muscle disease provides insights into filament organization Data size: 2.7 Data #1: Tilt-series of LmnaH222P/H222P revealing lamin meshwork in nuclease treated and FIB-milled nucleus [tilt series])
Download / File: emd_13056.map.gz / Format: CCP4 / Size: 165.7 MB / Type: IMAGE STORED AS SIGNED BYTE
Annotation
A tomogram of nuclease treated shvim HO H222P MEF nucleus. The tomogram is dose-weighted, CTF-corrected and reconstructed in IMOD. Fudicial markers are replaced by noise.
Voxel size
X=Y=Z: 8.82603 Å
Density
Minimum - Maximum
-128.0 - 127.0
Average (Standard dev.)
22.722765 (±17.823496)
Symmetry
Space group: 1
Details
EMDB XML:
Map geometry
Axis order
X
Y
Z
Origin
16
-16
-98
Dimensions
928
960
195
Spacing
960
928
195
Cell
A: 8472.988 Å / B: 8190.5557 Å / C: 1721.0758 Å α=β=γ: 90.0 °
CCP4 map header:
mode
envelope stored as signed bytes (from -128 lowest to 127 highest)
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