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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-12662 | |||||||||
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Title | Cryo-EM structure of the MukBEF-MatP-DNA tetrad | |||||||||
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Function / homology | ![]() nucleoid / chromosome condensation / acyl carrier activity / chromosome segregation / DNA replication / sequence-specific DNA binding / cell division / calcium ion binding / regulation of DNA-templated transcription / DNA binding ...nucleoid / chromosome condensation / acyl carrier activity / chromosome segregation / DNA replication / sequence-specific DNA binding / cell division / calcium ion binding / regulation of DNA-templated transcription / DNA binding / ATP binding / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 11.0 Å | |||||||||
![]() | Buermann F / Lowe J | |||||||||
![]() | ![]() Title: Cryo-EM structure of MukBEF reveals DNA loop entrapment at chromosomal unloading sites. Authors: Frank Bürmann / Louise F H Funke / Jason W Chin / Jan Löwe / ![]() Abstract: The ring-like structural maintenance of chromosomes (SMC) complex MukBEF folds the genome of Escherichia coli and related bacteria into large loops, presumably by active DNA loop extrusion. MukBEF ...The ring-like structural maintenance of chromosomes (SMC) complex MukBEF folds the genome of Escherichia coli and related bacteria into large loops, presumably by active DNA loop extrusion. MukBEF activity within the replication terminus macrodomain is suppressed by the sequence-specific unloader MatP. Here, we present the complete atomic structure of MukBEF in complex with MatP and DNA as determined by electron cryomicroscopy (cryo-EM). The complex binds two distinct DNA double helices corresponding to the arms of a plectonemic loop. MatP-bound DNA threads through the MukBEF ring, while the second DNA is clamped by the kleisin MukF, MukE, and the MukB ATPase heads. Combinatorial cysteine cross-linking confirms this topology of DNA loop entrapment in vivo. Our findings illuminate how a class of near-ubiquitous DNA organizers with important roles in genome maintenance interacts with the bacterial chromosome. #1: ![]() Title: DNA entrapment revealed by the structure of bacterial condensin MukBEF Authors: Buermann F / Funke LFH / Chin JW / Lowe J | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 29.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 24.7 KB 24.7 KB | Display Display | ![]() |
Images | ![]() | 48.1 KB | ||
Masks | ![]() | 38.4 MB | ![]() | |
Others | ![]() ![]() | 29.7 MB 29.7 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7nz2MC ![]() 7nywC ![]() 7nyxC ![]() 7nyyC ![]() 7nyzC ![]() 7nz0C ![]() 7nz3C ![]() 7nz4C M: atomic model generated by this map C: citing same article ( |
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Similar structure data | |
EM raw data | ![]() Data #1: Unaligned multi-frame images of MukBEF(E1407Q)-MatP-DNA in the presence of ATP - Dataset 1 [micrographs - multiframe] Data #2: Unaligned multi-frame images of MukBEF(E1407Q)-MatP-DNA in the presence of ATP - Dataset 2 [micrographs - multiframe] Data #3: Unaligned multi-frame images of MukBEF(E1407Q)-MatP-DNA in the presence of ATP - Dataset 3, grid 1 [micrographs - multiframe] Data #4: Unaligned multi-frame images of MukBEF(E1407Q)-MatP-DNA in the presence of ATP - Dataset 3, grid 2 [micrographs - multiframe] Data #5: Unaligned multi-frame images of MukBEF(E1407Q)-MatP-DNA in the presence of ATP - Dataset 3, grid 3 [micrographs - multiframe]) |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 4.28 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: Half map 2
File | emd_12662_half_map_1.map | ||||||||||||
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Annotation | Half map 2 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map 1
File | emd_12662_half_map_2.map | ||||||||||||
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Annotation | Half map 1 | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
+Entire : Complex of MukBEF, AcpP, MatP and DNA
+Supramolecule #1: Complex of MukBEF, AcpP, MatP and DNA
+Supramolecule #2: Complex of MukBEFand MatP
+Supramolecule #3: Acyl carrier protein
+Supramolecule #4: DNA
+Macromolecule #1: Chromosome partition protein MukB
+Macromolecule #2: Chromosome partition protein MukF
+Macromolecule #3: Chromosome partition protein MukE
+Macromolecule #4: Acyl carrier protein
+Macromolecule #5: Macrodomain Ter protein
+Macromolecule #6: matS2 DNA 80 b, oligo FBA769
+Macromolecule #7: matS2 DNA 80 b, oligo FBA770
+Macromolecule #8: MAGNESIUM ION
+Macromolecule #9: ADENOSINE-5'-TRIPHOSPHATE
+Macromolecule #10: 4'-PHOSPHOPANTETHEINE
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.3 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 40.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 11.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 12010 |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |