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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Plasmodium falciparum Glutamine synthetase (PfGs) | |||||||||
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Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / negative staining / Resolution: 2.8 Å | |||||||||
![]() | Makumire S / Mlelu AE / Gqunu S / Weber WB / Sewell BT | |||||||||
Funding support | ![]()
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![]() | ![]() Title: The structure of glutamine synthetase from Plasmodium falciparum Authors: Makumire S / Gqunu S / Mlelu AE / Weber WB / Sewell BT | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 28.8 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14.3 KB 14.3 KB | Display Display | ![]() |
Images | ![]() | 113 KB | ||
Masks | ![]() | 30.5 MB | ![]() | |
Others | ![]() ![]() | 28.3 MB 28.3 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 606 KB | Display | ![]() |
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Full document | ![]() | 605.6 KB | Display | |
Data in XML | ![]() | 10.8 KB | Display | |
Data in CIF | ![]() | 12.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
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Links
EMDB pages | ![]() ![]() |
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Map
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Voxel size | X=Y=Z: 1.04 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_12589_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_12589_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : P falciparum Glutamine synthetase
Entire | Name: P falciparum Glutamine synthetase |
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Components |
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-Supramolecule #1: P falciparum Glutamine synthetase
Supramolecule | Name: P falciparum Glutamine synthetase / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Experimental: 756 KDa |
Recombinant expression | Organism: ![]() ![]() |
-Macromolecule #1: Glutamine synthetase
Macromolecule | Name: Glutamine synthetase / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MLIKLFFSNN AELYEYIKDK KNDVEIVACI ITNLLGTYFK CFFYVKEITL NKLESGFSFD ASSIKLCSD TEVSDFFIKV DHSTCYLEEC DGKNILNIMC DIKRYNGFDY YKCPRTILKK T CEFVKNEG IADKVCIGNE LEFFIFDKVN YSLDEYNTYL KVYDRESFSC ...String: MLIKLFFSNN AELYEYIKDK KNDVEIVACI ITNLLGTYFK CFFYVKEITL NKLESGFSFD ASSIKLCSD TEVSDFFIKV DHSTCYLEEC DGKNILNIMC DIKRYNGFDY YKCPRTILKK T CEFVKNEG IADKVCIGNE LEFFIFDKVN YSLDEYNTYL KVYDRESFSC KNDLSSIYGN HV VNKVEPH KDHFNNPNNE YLINDDSKKV KKKSGYFTTD PYDTSNIIKL RICRALNDMN INV QRYHHE VSTSQHEISL KYFDALTNAD FLLITKQIIK TTVSSFNRTA TFMPKPLVND NGNG LHCNI SLWKNNKNIF YHNDPSTFFL SKESFYFMYG IVKHAKALQA FCNATMNSYK RLVPG FETC QKLFYSFGSR SAVIRLSLIN YSNPSEKRIE FRLPDCANSP HLVMAAIILA GYDGIK SKE QPLVPFESKD NHFYISSIFS KYVQHPENFN ILTHALEGYE SLHTINESPE FKNFFKC EE PQGISFSLVE SLDALEKDHA FLTVNNIFTE EMIQEYIKFK REEIDAYNKY VNAYDYHL Y YEC |
-Experimental details
-Structure determination
Method | negative staining, cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 |
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Staining | Type: NEGATIVE / Material: uranyl acetate |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 55.34 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Refinement | Space: RECIPROCAL / Protocol: AB INITIO MODEL |
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