ジャーナル: Sci Adv / 年: 2021 タイトル: A dynein-associated photoreceptor protein prevents ciliary acclimation to blue light. 著者: Osamu Kutomi / Ryosuke Yamamoto / Keiko Hirose / Katsutoshi Mizuno / Yuuhei Nakagiri / Hiroshi Imai / Akira Noga / Jagan Mohan Obbineni / Noemi Zimmermann / Masako Nakajima / Daisuke Shibata ...著者: Osamu Kutomi / Ryosuke Yamamoto / Keiko Hirose / Katsutoshi Mizuno / Yuuhei Nakagiri / Hiroshi Imai / Akira Noga / Jagan Mohan Obbineni / Noemi Zimmermann / Masako Nakajima / Daisuke Shibata / Misa Shibata / Kogiku Shiba / Masaki Kita / Hideo Kigoshi / Yui Tanaka / Yuya Yamasaki / Yuma Asahina / Chihong Song / Mami Nomura / Mamoru Nomura / Ayako Nakajima / Mia Nakachi / Lixy Yamada / Shiori Nakazawa / Hitoshi Sawada / Kazuyoshi Murata / Kaoru Mitsuoka / Takashi Ishikawa / Ken-Ichi Wakabayashi / Takahide Kon / Kazuo Inaba / 要旨: Light-responsive regulation of ciliary motility is known to be conducted through modulation of dyneins, but the mechanism is not fully understood. Here, we report a novel subunit of the two-headed ...Light-responsive regulation of ciliary motility is known to be conducted through modulation of dyneins, but the mechanism is not fully understood. Here, we report a novel subunit of the two-headed f/I1 inner arm dynein, named DYBLUP, in animal spermatozoa and a unicellular green alga. This subunit contains a BLUF (sensors of blue light using FAD) domain that appears to directly modulate dynein activity in response to light. DYBLUP (dynein-associated BLUF protein) mediates the connection between the f/I1 motor domain and the tether complex that links the motor to the doublet microtubule. lacking the DYBLUP ortholog shows both positive and negative phototaxis but becomes acclimated and attracted to high-intensity blue light. These results suggest a mechanism to avoid toxic strong light via direct photoregulation of dyneins.
ダウンロード / ファイル: emd_12161.map.gz / 形式: CCP4 / 大きさ: 16.8 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
cilia from Chlamydomonas with MOT7 proteins tagged with BCCP and labeled
ボクセルのサイズ
X=Y=Z: 8.4 Å
密度
表面レベル
登録者による: 3900.0 / ムービー #1: 3900
最小 - 最大
0.0 - 10000.0
平均 (標準偏差)
3702.3315 (±641.7208)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
-58
0
0
サイズ
164
164
164
Spacing
164
164
164
セル
A=B=C: 1377.6 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
8.4
8.4
8.4
M x/y/z
164
164
164
origin x/y/z
0.000
0.000
0.000
length x/y/z
1377.600
1377.600
1377.600
α/β/γ
90.000
90.000
90.000
MAP C/R/S
1
2
3
start NC/NR/NS
0
-58
0
NC/NR/NS
164
164
164
D min/max/mean
0.000
10000.000
3702.332
-
添付データ
-
試料の構成要素
-
全体 : doublet microtubules from cilia
全体
名称: doublet microtubules from cilia
要素
細胞器官・細胞要素: doublet microtubules from cilia
-
超分子 #1: doublet microtubules from cilia
超分子
名称: doublet microtubules from cilia / タイプ: organelle_or_cellular_component / ID: 1 / 親要素: 0 詳細: Average of subtomograms extracted from microtubule doublet parts of cilia