+
データを開く
-
基本情報
登録情報 | データベース: EMDB / ID: EMD-12081 | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
タイトル | Yeast 80S ribosome bound to eEF3 and A/A- and P/P-tRNAs (PRE-1) | |||||||||||||||
![]() | full 80S Map Post-Process | |||||||||||||||
![]() |
| |||||||||||||||
![]() | eukaryotic Elongation Factor 3 / eEF3 / YEF3 / Translation / Elongation / ABCF | |||||||||||||||
機能・相同性 | ![]() cytoplasmic translational elongation / cytoplasmic translational termination / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / Negative regulators of DDX58/IFIH1 signaling / regulation of amino acid metabolic process / negative regulation of glucose mediated signaling pathway / positive regulation of translational fidelity / RMTs methylate histone arginines / Protein methylation / mTORC1-mediated signalling ...cytoplasmic translational elongation / cytoplasmic translational termination / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / Negative regulators of DDX58/IFIH1 signaling / regulation of amino acid metabolic process / negative regulation of glucose mediated signaling pathway / positive regulation of translational fidelity / RMTs methylate histone arginines / Protein methylation / mTORC1-mediated signalling / Protein hydroxylation / ribosome-associated ubiquitin-dependent protein catabolic process / GDP-dissociation inhibitor activity / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / pre-mRNA 5'-splice site binding / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / preribosome, small subunit precursor / nonfunctional rRNA decay / response to cycloheximide / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Major pathway of rRNA processing in the nucleolus and cytosol / mRNA destabilization / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / negative regulation of translational frameshifting / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / Formation of a pool of free 40S subunits / preribosome, large subunit precursor / L13a-mediated translational silencing of Ceruloplasmin expression / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translational elongation / ribosomal large subunit export from nucleus / 90S preribosome / positive regulation of protein kinase activity / G-protein alpha-subunit binding / Ub-specific processing proteases / translation elongation factor activity / ribosomal subunit export from nucleus / regulation of translational fidelity / protein-RNA complex assembly / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translational termination / maturation of LSU-rRNA / ribosomal small subunit export from nucleus / translation regulator activity / cytosolic ribosome / DNA-(apurinic or apyrimidinic site) endonuclease activity / rescue of stalled ribosome / cellular response to amino acid starvation / ribosome assembly / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / protein kinase C binding / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / translational initiation / small-subunit processome / macroautophagy / maintenance of translational fidelity / 加水分解酵素; 酸無水物に作用; 酸無水物に作用・細胞または細胞小器官の運動に関与 / modification-dependent protein catabolic process / protein tag activity / cytoplasmic stress granule / rRNA processing / ribosome biogenesis / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / protein ubiquitination / ribosome / structural constituent of ribosome / G protein-coupled receptor signaling pathway / translation / negative regulation of gene expression / response to antibiotic / mRNA binding / ubiquitin protein ligase binding / nucleolus / ATP hydrolysis activity / mitochondrion / RNA binding / zinc ion binding / nucleoplasm / ATP binding / nucleus / cytosol 類似検索 - 分子機能 | |||||||||||||||
生物種 | ![]() ![]() ![]() ![]() ![]() ![]() | |||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.3 Å | |||||||||||||||
![]() | Ranjan N / Pochopien AA / Wu CC / Beckert B / Blanchet S / Green R / Rodnina MV / Wilson DN | |||||||||||||||
資金援助 | ![]()
| |||||||||||||||
![]() | ![]() タイトル: eEF3 promotes late stages of tRNA translocation including E-tRNA release from the ribosome 著者: Ranjan N / Pochopien AA / Wu CC / Beckert B / Blanchet S / Green R / Rodnina MV / Wilson DN | |||||||||||||||
履歴 |
|
-
構造の表示
ムービー |
![]() |
---|---|
構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
-
ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 23.4 MB | ![]() | |
---|---|---|---|---|
ヘッダ (付随情報) | ![]() ![]() | 130.6 KB 130.6 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 14.9 KB | 表示 | ![]() |
画像 | ![]() | 93.1 KB | ||
Filedesc metadata | ![]() | 19.8 KB | ||
その他 | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | 264.8 MB 264.4 MB 17.1 MB 265 MB 264.8 MB 264.7 MB 224.8 MB 7.7 MB 4.7 MB 264.4 MB 225.3 MB 226 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 906.5 KB | 表示 | ![]() |
---|---|---|---|---|
文書・詳細版 | ![]() | 906.2 KB | 表示 | |
XML形式データ | ![]() | 22.6 KB | 表示 | |
CIF形式データ | ![]() | 29.7 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
-
リンク
EMDBのページ | ![]() ![]() |
---|---|
「今月の分子」の関連する項目 |
-
マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
注釈 | full 80S Map Post-Process | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.084 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
|
-添付データ
+追加マップ: full Map Post-Process lowpass filtered to 5 Angstrom
+追加マップ: Multibody Small subunit Head region Map Post-Process lowpass...
+追加マップ: Multibody Large subunit Post-process
+追加マップ: full Map Post-Process lowpass filtered to 4 Angstrom
+追加マップ: Multibody Large subunit Map Post-Process lowpass filtered to...
+追加マップ: Multibody Large subunit Map Post-Process lowpass filtered to...
+追加マップ: Relion final refinement round
+追加マップ: Multibody Small subunit body region Map Post-Process
+追加マップ: Multibody Small subunit Head region Map Post-Process
+追加マップ: Multibody Small subunit Head region Map Post-Process lowpass...
+ハーフマップ: Half map
+ハーフマップ: Half map
-
試料の構成要素
+全体 : Yeast 80S ribosome bound to eEF3 and A/A- and P/P-tRNAs
+超分子 #1: Yeast 80S ribosome bound to eEF3 and A/A- and P/P-tRNAs
+分子 #1: 18S rRNA
+分子 #2: mRNA
+分子 #36: 25S rRNA
+分子 #37: 5S rRNA
+分子 #38: 5.8S rRNA
+分子 #80: tRNA
+分子 #3: 40S ribosomal protein S0-A
+分子 #4: 40S ribosomal protein S1-A
+分子 #5: 40S ribosomal protein S15
+分子 #6: 40S ribosomal protein S2
+分子 #7: 40S ribosomal protein S3
+分子 #8: 40S ribosomal protein S4-A
+分子 #9: 40S ribosomal protein S5
+分子 #10: 40S ribosomal protein S6-A
+分子 #11: 40S ribosomal protein S7-A
+分子 #12: 40S ribosomal protein S8-A
+分子 #13: 40S ribosomal protein S9-A
+分子 #14: 40S ribosomal protein S10-A
+分子 #15: 40S ribosomal protein S11-A
+分子 #16: 40S ribosomal protein S12
+分子 #17: 40S ribosomal protein S13
+分子 #18: 40S ribosomal protein S14-B
+分子 #19: 40S ribosomal protein S16-A
+分子 #20: 40S ribosomal protein S17-B
+分子 #21: 40S ribosomal protein S18-A
+分子 #22: 40S ribosomal protein S19-A
+分子 #23: 40S ribosomal protein S20
+分子 #24: 40S ribosomal protein S21-A
+分子 #25: 40S ribosomal protein S22-A
+分子 #26: 40S ribosomal protein S23-A
+分子 #27: 40S ribosomal protein S24-A
+分子 #28: 40S ribosomal protein S25-A
+分子 #29: 40S ribosomal protein S26-B
+分子 #30: 40S ribosomal protein S27-A
+分子 #31: 40S ribosomal protein S29-A
+分子 #32: 40S ribosomal protein S30-A
+分子 #33: Ubiquitin-40S ribosomal protein S31
+分子 #34: Guanine nucleotide-binding protein subunit beta-like protein
+分子 #35: 40S ribosomal protein S28-A
+分子 #39: 60S ribosomal protein L2-A
+分子 #40: 60S ribosomal protein L3
+分子 #41: 60S ribosomal protein L4-A
+分子 #42: 60S ribosomal protein L5
+分子 #43: 60S ribosomal protein L6-B
+分子 #44: 60S ribosomal protein L7-A
+分子 #45: 60S ribosomal protein L8-A
+分子 #46: 60S ribosomal protein L9-A
+分子 #47: 60S ribosomal protein L10
+分子 #48: 60S ribosomal protein L11-B
+分子 #49: 60S ribosomal protein L13-A
+分子 #50: 60S ribosomal protein L14-A
+分子 #51: 60S ribosomal protein L15-A
+分子 #52: 60S ribosomal protein L16-A
+分子 #53: 60S ribosomal protein L17-A
+分子 #54: 60S ribosomal protein L18-A
+分子 #55: 60S ribosomal protein L19-A
+分子 #56: 60S ribosomal protein L20-A
+分子 #57: 60S ribosomal protein L21-A
+分子 #58: 60S ribosomal protein L22-A
+分子 #59: 60S ribosomal protein L23-A
+分子 #60: 60S ribosomal protein L24-A
+分子 #61: 60S ribosomal protein L25
+分子 #62: 60S ribosomal protein L26-A
+分子 #63: 60S ribosomal protein L27-A
+分子 #64: 60S ribosomal protein L28
+分子 #65: 60S ribosomal protein L29
+分子 #66: 60S ribosomal protein L30
+分子 #67: 60S ribosomal protein L31-A
+分子 #68: 60S ribosomal protein L32
+分子 #69: 60S ribosomal protein L33-A
+分子 #70: 60S ribosomal protein L34-A
+分子 #71: 60S ribosomal protein L35-A
+分子 #72: 60S ribosomal protein L36-A
+分子 #73: 60S ribosomal protein L37-A
+分子 #74: 60S ribosomal protein L38
+分子 #75: 60S ribosomal protein L39
+分子 #76: Ubiquitin-60S ribosomal protein L40
+分子 #77: 60S ribosomal protein L41-B
+分子 #78: 60S ribosomal protein L42-A
+分子 #79: 60S ribosomal protein L43-A
+分子 #81: Elongation factor 3A
+分子 #82: ADENOSINE-5'-TRIPHOSPHATE
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
---|---|
![]() | 単粒子再構成法 |
試料の集合状態 | particle |
-
試料調製
緩衝液 | pH: 6.5 |
---|---|
凍結 | 凍結剤: ETHANE-PROPANE |
-
電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
---|---|
撮影 | フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 平均電子線量: 25.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: SPOT SCAN / 撮影モード: BRIGHT FIELD |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |