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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Cryo-EM of Aedes Aegypti Toll5A dimer bound to Spz1C | |||||||||
![]() | EM map | |||||||||
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Function / homology | ![]() toll-like receptor signaling pathway / transmembrane signaling receptor activity / inflammatory response / innate immune response / extracellular space / membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.23 Å | |||||||||
![]() | Gangloff M / Hardwick SW / Chirgadze DY | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure and dynamics of Toll immunoreceptor activation in the mosquito Aedes aegypti. Authors: Yoann Saucereau / Thomas H Wilson / Matthew C K Tang / Martin C Moncrieffe / Steven W Hardwick / Dimitri Y Chirgadze / Sandro G Soares / Maria Jose Marcaida / Nicholas J Gay / Monique Gangloff / ![]() ![]() Abstract: Aedes aegypti has evolved to become an efficient vector for arboviruses but the mechanisms of host-pathogen tolerance are unknown. Immunoreceptor Toll and its ligand Spaetzle have undergone ...Aedes aegypti has evolved to become an efficient vector for arboviruses but the mechanisms of host-pathogen tolerance are unknown. Immunoreceptor Toll and its ligand Spaetzle have undergone duplication which may allow neofunctionalization and adaptation. Here we present cryo-EM structures and biophysical characterisation of low affinity Toll5A complexes that display transient but specific interactions with Spaetzle1C, forming asymmetric complexes, with only one ligand clearly resolved. Loop structures of Spaetzle1C and Toll5A intercalate, temporarily bridging the receptor C-termini to promote signalling. By contrast unbound receptors form head-to-head homodimers that keep the juxtamembrane regions far apart in an inactive conformation. Interestingly the transcriptional signature of Spaetzle1C differs from other Spaetzle cytokines and controls genes involved in innate immunity, metabolism and tissue regeneration. Taken together our results explain how upregulation of Spaetzle1C in the midgut and Toll5A in the salivary gland shape the concomitant immune response. | |||||||||
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 103.9 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 20.9 KB 20.9 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 17.5 KB | Display | ![]() |
Images | ![]() | 117.3 KB | ||
Others | ![]() ![]() | 194.2 MB 194.2 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7b1bMC ![]() 7b1cC ![]() 7b1dC C: citing same article ( M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | EM map | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.83 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Half map
File | emd_11982_half_map_1.map | ||||||||||||
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Annotation | Half map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map
File | emd_11982_half_map_2.map | ||||||||||||
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Annotation | Half map | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Cryo-EM of Aedes Aegypti Toll5A dimer bound to Spz1C
Entire | Name: Cryo-EM of Aedes Aegypti Toll5A dimer bound to Spz1C |
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Components |
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-Supramolecule #1: Cryo-EM of Aedes Aegypti Toll5A dimer bound to Spz1C
Supramolecule | Name: Cryo-EM of Aedes Aegypti Toll5A dimer bound to Spz1C / type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: #1-#2 |
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Molecular weight | Theoretical: 230 KDa |
-Supramolecule #2: Toll-like receptor
Supramolecule | Name: Toll-like receptor / type: complex / ID: 2 / Chimera: Yes / Parent: 1 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
-Supramolecule #3: AAEL013433-PA
Supramolecule | Name: AAEL013433-PA / type: complex / ID: 3 / Chimera: Yes / Parent: 1 / Macromolecule list: #2 |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: Toll-like receptor
Macromolecule | Name: Toll-like receptor / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 87.675773 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: TSTKRFTCPE ESEASNCSCE EFPSKTHFYC PDFNPTLYVD VEDRMRVDFK CYDEPHDFKS LPNLAIGSVK LLTVVDCVLD DDRPILESF KFLEVADVRS FVYNNHENGI RYNAKYFEGM EQLENLTLAR GVVSIDRDTF SGFLNLKRLT IEHNKLNLQP G TFEALSNL ...String: TSTKRFTCPE ESEASNCSCE EFPSKTHFYC PDFNPTLYVD VEDRMRVDFK CYDEPHDFKS LPNLAIGSVK LLTVVDCVLD DDRPILESF KFLEVADVRS FVYNNHENGI RYNAKYFEGM EQLENLTLAR GVVSIDRDTF SGFLNLKRLT IEHNKLNLQP G TFEALSNL TYLGLVYNGL NEIQPGLFDG LESLEALSLS YNDIKSLSAG SFNGLSSLRM LNLRVNKIES FDANTFASLK EL SRLEITL NPFVSLPRGL FSENKKLKTL ILTNNRKLVT LPEELLANLK ELTVVNLSHN GVGNLPESLL SGSSGIIELN LGY NRLNSL PEELLSDQPQ LQVLNLDHNQ LESIPDYFLE RNVELQTLYL SHNRLRSLSE KAFTKLKNLK ELHLENNQLQ TIPQ FLFSG TPKLEEIYMQ NNQLALHANS FINEELSIAD NDNTPFQVLQ KLRILHLRNN SISTIFQDWY INNLEMQSLD LSFNK LPGL SYTQLQFQSN ITLNLSNNEI SQVLLIDDLD LQPYQRINVD LNHNPLNCNC NALKFIQLIQ SKAEHGLQFN VDQLRC SEP PNLLDATMDQ LQTKDLLCDF ESADDCPKDC QCAMRLLDHT VIVNCSGRGL TEFPDLPIPS QLHEDFNALE VHVENNR LT KLPNLTKHNE ITQLYARNNS IQNLLPHNIP SKLRIIDLSQ NLLKMIDDST LAQINRSSHL ETIRLSQNQW LCDCPASS F LIFVQQNSRL ISDMSAIRCH PSGKSLDSIT VNELCFEDYT TENLYFQ |
-Macromolecule #2: AAEL013433-PA
Macromolecule | Name: AAEL013433-PA / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 12.941044 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: SDTANAPFLC ESEQLLIHPK EELSRNNSMV WIVNTKDYKQ GVRIEKCLKR QLGKPCNFCD ADTECKQLFH YRTLVAVDKV TKKPYKEQV LLPSCCKCAK ILSTGWSHPQ FEK |
-Macromolecule #5: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 5 / Number of copies: 3 / Formula: NAG |
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Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ![]() ChemComp-NAG: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 5 mg/mL | |||||||||
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Buffer | pH: 7.5 Component:
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Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 12.0 nm / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 60 sec. | |||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK I / Details: Blotting force 0. |
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Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average exposure time: 2.0 sec. / Average electron dose: 51.1 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.7 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 105000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |