Journal: Nat Commun / Year: 2021 Title: Self-assembly and regulation of protein cages from pre-organised coiled-coil modules. Authors: Fabio Lapenta / Jana Aupič / Marco Vezzoli / Žiga Strmšek / Stefano Da Vela / Dmitri I Svergun / José María Carazo / Roberto Melero / Roman Jerala / Abstract: Coiled-coil protein origami (CCPO) is a modular strategy for the de novo design of polypeptide nanostructures. CCPO folds are defined by the sequential order of concatenated orthogonal coiled-coil ...Coiled-coil protein origami (CCPO) is a modular strategy for the de novo design of polypeptide nanostructures. CCPO folds are defined by the sequential order of concatenated orthogonal coiled-coil (CC) dimer-forming peptides, where a single-chain protein is programmed to fold into a polyhedral cage. Self-assembly of CC-based nanostructures from several chains, similarly as in DNA nanotechnology, could facilitate the design of more complex assemblies and the introduction of functionalities. Here, we show the design of a de novo triangular bipyramid fold comprising 18 CC-forming segments and define the strategy for the two-chain self-assembly of the bipyramidal cage from asymmetric and pseudo-symmetric pre-organised structural modules. In addition, by introducing a protease cleavage site and masking the interfacial CC-forming segments in the two-chain bipyramidal cage, we devise a proteolysis-mediated conformational switch. This strategy could be extended to other modular protein folds, facilitating the construction of dynamic multi-chain CC-based complexes.
History
Deposition
Oct 12, 2020
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Header (metadata) release
Dec 23, 2020
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Map release
Dec 23, 2020
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Update
Nov 29, 2023
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Current status
Nov 29, 2023
Processing site: PDBe / Status: Released
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Surface view with section colored by density value
Samples were applied to glow discharged carbon-coated copper grids, washed quickly with distilled water and negatively stained with 2% (w/v) uranyl acetate.
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Electron microscopy
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JEOL 1230
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Film or detector model: TVIPS TEMCAM-F415 (4k x 4k) / Average electron dose: 20.0 e/Å2
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Acceleration voltage: 120 kV / Electron source: TUNGSTEN HAIRPIN
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