EMDB-11810: Cryo-EM structure of respiratory complex I in the deactive state ...

基本情報

• 登録情報: データベース: EMDB / ID: EMD-11810
• タイトル: Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A

試料

• 複合体: Mouse mitochondrial complex I
  • タンパク質・ペプチド: x 44種
• リガンド: x 10種

• キーワード: deactive complex I / OXIDOREDUCTASE

機能・相同性

分子機能:

Mitochondrial protein import / response to injury involved in regulation of muscle adaptation / mesenchymal stem cell proliferation / reproductive system development / Protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation / Complex I biogenesis / RHOG GTPase cycle / Respiratory electron transport / protein insertion into mitochondrial inner membrane / mesenchymal stem cell differentiation / circulatory system development / blastocyst hatching / respiratory system process / psychomotor behavior / Mitochondrial protein degradation / cellular response to oxygen levels / stem cell division / respiratory chain complex / response to light intensity / mitochondrial [2Fe-2S] assembly complex / iron-sulfur cluster assembly complex / mitochondrial large ribosomal subunit binding / gliogenesis / negative regulation of non-canonical NF-kappaB signal transduction / cardiac muscle tissue development / neural precursor cell proliferation / positive regulation of mitochondrial membrane potential / [2Fe-2S] cluster assembly / adult walking behavior / cellular response to glucocorticoid stimulus / oxygen sensor activity / response to hydroperoxide / iron-sulfur cluster assembly / positive regulation of ATP biosynthetic process / dopamine metabolic process / adult behavior / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I assembly / positive regulation of execution phase of apoptosis / mitochondrial electron transport, NADH to ubiquinone / NADH dehydrogenase activity / proton motive force-driven mitochondrial ATP synthesis / respiratory chain complex I / NADH dehydrogenase (ubiquinone) activity / neuron development / quinone binding / cellular response to interferon-beta / ATP synthesis coupled electron transport / cellular response to retinoic acid / negative regulation of reactive oxygen species biosynthetic process / extrinsic apoptotic signaling pathway / neurogenesis / tricarboxylic acid cycle / muscle contraction / Neutrophil degranulation / visual perception / cerebellum development / reactive oxygen species metabolic process / aerobic respiration / DNA damage response, signal transduction by p53 class mediator / regulation of mitochondrial membrane potential / kidney development / fatty acid metabolic process / respiratory electron transport chain / mitochondrion organization / response to nicotine / sensory perception of sound / response to cocaine / monooxygenase activity / electron transport chain / mitochondrial membrane / : / brain development / multicellular organism growth / response to hydrogen peroxide / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / cognition / circadian rhythm / positive regulation of protein catabolic process / NAD binding / fatty acid biosynthetic process / cellular senescence / FMN binding / nervous system development / myelin sheath / 4 iron, 4 sulfur cluster binding / neuron apoptotic process / gene expression / response to oxidative stress / in utero embryonic development / response to ethanol / response to hypoxia / electron transfer activity / mitochondrial inner membrane / nuclear speck / nuclear body / mitochondrial matrix / inflammatory response

ドメイン・相同性:

NmrA-like domain / NmrA-like family / Complex1_LYR-like / NADH-ubiquinone oxidoreductase 1 subunit C1 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH:ubiquinone oxidoreductase, ESSS subunit / ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1, NDUB1 / MNLL subunit / NADH-ubiquinone oxidoreductase flavoprotein 3 / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / Tim17/Tim22/Tim23/Pmp24 family / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, metazoa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, animal type / NADH dehydrogenase 1 beta subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUC2 / NADH:ubiquinone oxidoreductase, subunit NDUFB4 / NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2) / NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4) / NADH dehydrogenase 1, beta subcomplex, subunit 6 / NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit / : / NADH:ubiquinone oxidoreductase chain 4, N-terminal / NADH:ubiquinone oxidoreductase, chain 2 / NADH dehydrogenase subunit 2, C-terminal / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / : / : / NADH-ubiquinone oxidoreductase chain 4, amino terminus / NADH dehydrogenase subunit 2 C-terminus / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / : / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase MWFE subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / NADH-ubiquinone oxidoreductase NDSU1/NuoG-like, 4Fe-4S domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 / NADH:ubiquinone oxidoreductase subunit B14.5a (Complex I-B14.5a) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase 1 alpha subcomplex subunit 3 / Zinc finger, CHCC-type / Zinc-finger domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase subunit 5, C-terminal / NADH dehydrogenase subunit 5 C-terminus / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NADH:ubiquinone oxidoreductase, NDUFS5-15kDa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / GRIM-19 / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NDUFB9, LYR domain / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / NDUFA6, LYR domain / Deoxynucleoside kinase domain / Deoxynucleoside kinase / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal / : / SLBB domain / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / : / : / NADH-quinone oxidoreductase subunit 3, ferredoxin-like domain / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ / NADH-quinone oxidoreductase, chain M/4 / NADH-ubiquinone oxidoreductase chain 4L/K / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus / NAD(P)H-quinone oxidoreductase subunit D/H / NADH-quinone oxidoreductase, chain 5-like / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / NADH-ubiquinone oxidoreductase chain 4L/Mnh complex subunit C1-like / NADH-ubiquinone/plastoquinone oxidoreductase chain 4L / Respiratory chain NADH dehydrogenase 49 Kd subunit signature. / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3.

構成要素:

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial / NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 subunit C2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / Acyl carrier protein, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

• 生物種: Mus musculus (ハツカネズミ)
• 手法: 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.17 Å
• データ登録者: Yin Z / Bridges HR

資金援助

英国, 2件

Organization	Grant number	国
Medical Research Council (MRC, United Kingdom)	MC_U105663141	英国
Medical Research Council (MRC, United Kingdom)	MC_UU_00015/2	英国

• 引用: ジャーナル: Nat Commun / 年: 2021; タイトル: Structural basis for a complex I mutation that blocks pathological ROS production.; 著者: Zhan Yin / Nils Burger / Duvaraka Kula-Alwar / Dunja Aksentijević / Hannah R Bridges / Hiran A Prag / Daniel N Grba / Carlo Viscomi / Andrew M James / Amin Mottahedin / Thomas Krieg / Michael P Murphy / Judy Hirst / ; 要旨: Mitochondrial complex I is central to the pathological reactive oxygen species (ROS) production that underlies cardiac ischemia-reperfusion (IR) injury. ND6-P25L mice are homoplasmic for a disease-causing mtDNA point mutation encoding the P25L substitution in the ND6 subunit of complex I. The cryo-EM structure of ND6-P25L complex I revealed subtle structural changes that facilitate rapid conversion to the "deactive" state, usually formed only after prolonged inactivity. Despite its tendency to adopt the "deactive" state, the mutant complex is fully active for NADH oxidation, but cannot generate ROS by reverse electron transfer (RET). ND6-P25L mitochondria function normally, except for their lack of RET ROS production, and ND6-P25L mice are protected against cardiac IR injury in vivo. Thus, this single point mutation in complex I, which does not affect oxidative phosphorylation but renders the complex unable to catalyse RET, demonstrates the pathological role of ROS production by RET during IR injury.

履歴

登録	2020年9月29日	-
ヘッダ(付随情報) 公開	2021年2月3日	-
マップ公開	2021年2月3日	-
更新	2025年4月9日	-
現状	2025年4月9日	処理サイト: PDBe / 状態: 公開

マップ

• ファイル: ダウンロード / ファイル: emd_11810.map.gz / 形式: CCP4 / 大きさ: 178 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• ボクセルのサイズ: X=Y=Z: 1.35 Å

密度

表面レベル	登録者による: 0.0355 / ムービー #1: 0.0355
最小 - 最大	-0.107197456 - 0.27112377
平均 (標準偏差)	-0.000023339951 (±0.007787233)

• 対称性: 空間群: 1

詳細

EMDB XML:

マップ形状	Axis order X Y Z Origin 0 0 0 サイズ 360 360 360 Spacing 360 360 360
セル	A=B=C: 486.0 Å α=β=γ: 90.0 °

CCP4マップ ヘッダ情報:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.35	1.35	1.35
M x/y/z	360	360	360
origin x/y/z	0.000	0.000	0.000
length x/y/z	486.000	486.000	486.000
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	360	360	360
D min/max/mean	-0.107	0.271	-0.000

添付データ

マスク #1

• ファイル: emd_11810_msk_1.map

追加マップ: Focussed refinement:Multibody3

• ファイル: emd_11810_additional_1.map
• 注釈: Focussed refinement:Multibody3

追加マップ: Focussed refinement:Multibody1

• ファイル: emd_11810_additional_2.map
• 注釈: Focussed refinement:Multibody1

追加マップ: Focussed refinement:Multibody2

• ファイル: emd_11810_additional_3.map
• 注釈: Focussed refinement:Multibody2

ハーフマップ: #2

• ファイル: emd_11810_half_map_1.map

ハーフマップ: #1

• ファイル: emd_11810_half_map_2.map

試料の構成要素

全体 : Mouse mitochondrial complex I

• 全体: 名称: Mouse mitochondrial complex I

要素

• 複合体: Mouse mitochondrial complex I
  • タンパク質・ペプチド: NADH-ubiquinone oxidoreductase chain 3
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
  • タンパク質・ペプチド: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
  • タンパク質・ペプチド: NADH-ubiquinone oxidoreductase chain 1
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
  • タンパク質・ペプチド: NADH-ubiquinone oxidoreductase chain 6
  • タンパク質・ペプチド: NADH-ubiquinone oxidoreductase chain 4L
  • タンパク質・ペプチド: NADH-ubiquinone oxidoreductase chain 5
  • タンパク質・ペプチド: NADH-ubiquinone oxidoreductase chain 4
  • タンパク質・ペプチド: NADH-ubiquinone oxidoreductase chain 2
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
  • タンパク質・ペプチド: Acyl carrier protein, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
  • タンパク質・ペプチド: MCG5603
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 subunit C2
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
  • タンパク質・ペプチド: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial
• リガンド: IRON/SULFUR CLUSTER
• リガンド: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
• リガンド: FE2/S2 (INORGANIC) CLUSTER
• リガンド: FLAVIN MONONUCLEOTIDE
• リガンド: 1,2-Distearoyl-sn-glycerophosphoethanolamine
• リガンド: CARDIOLIPIN
• リガンド: ADENOSINE-5'-TRIPHOSPHATE
• リガンド: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
• リガンド: ZINC ION
• リガンド: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate

超分子 #1: Mouse mitochondrial complex I

• 超分子: 名称: Mouse mitochondrial complex I / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#44 / 詳細: complex I in the deactive state
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 株: C57BL/6J / 器官: heart / Organelle: Mitochondrion
• 分子量: 理論値: 1 MDa

分子 #1: NADH-ubiquinone oxidoreductase chain 3

• 分子: 名称: NADH-ubiquinone oxidoreductase chain 3 / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 13.251785 KDa

配列

文字列:

(FME)NLYTVIFIN ILLSLTLILV AFWLPQMNLY SEKANPYECG FDPTSSARLP FSMKFFLVAI TFLLFDLEIA LLLPLP WAI QTIKTSTMMI MAFILVTILS LGLAYEWTQK GLEWTE

UniProtKB: NADH-ubiquinone oxidoreductase chain 3

分子 #2: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial; タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 24.715912 KDa

配列

文字列:

MAALAAPGLL SVRILGLRTA QVQLRRVHQS VATEGPSPSP SPSLSSTQSA VSKAGAGAVV PKLSHLPRSR AEYVVTKLDD LINWARRSS LWPMTFGLAC CAVEMMHMAA PRYDMDRFGV VFRASPRQAD VMIVAGTLTN KMAPALRKVY DQMPEPRYVV S MGSCANGG GYYHYSYSVV RGCDRIVPVD IYVPGCPPTA EALLYGILQL QRKIKREQKL KIWYRR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

分子 #3: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial; タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 30.191307 KDa

配列

文字列:

MAAAAARVWC RGLLGAASVG RGAGRPSVLW QHVRRESAAA DKRPTVRPRS DVTHKQLSAF GEYVAEILPK YVQQVQVSCL DELEICIHP DGVIPTLTFL RDHTNAQFKS LADLTAVDVP TRQNRFEIVY NLLSLRFNSR IRVKTYADEL TPIDSIVSVH I AANWYERE VWDMFGVFFF NHPDLRRILT DYGFEGHPFR KDFPLTGYVE LRYDDEVKRV VAEPVELAQE FRKFDLNSPW EA FPAYRQP PESLKLEAGD KKPETK

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

分子 #4: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial; タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 52.720602 KDa

配列

文字列:

MAALRALRCL RGVGAPVLRP GSGIRLPSQP SRGARQWQPD IEWAEQFSGA VMYPSKETAH WKPPPWNDVD ILKEKAVTNM TLNFGPQHP AAHGVLRLVL ELSGEMVRKC DPHIGLLH(2MR)G TEKLIEYKTY LQALPYFDRL DYVSMMCNEQ AYSIAVE KL LNIQPPPRAQ WIRVLFGEIT RILNHIMAVT THALDIGAMT PFFWMFEERE KMFEFYERVS GARMHAAYIR PGGVHQDL P LGLLDDIYEF SKNFSLRIDE VEEMLTNNRI WRNRTVDIGV VTAEDALNYG FSGVMLRGSG IQWDLRKTQP YDVYDQVEF DVPIGSRGDC YDRYLCRVEE MRQSLRIIEQ CLNKMPPGEI KVDDAKVSPP KRAEMKTSME SLIHHFKLYT EGYQVPPGAT YTAIEAPKG EFGVYLVSDG SSRPYRCKIK APGFAHLAGL DKMSKGHMLA DVVAIIGTQD IVFGEIDR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

分子 #5: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 27.077047 KDa

配列

文字列:

MFSLALRARA TGLAAQWGRH ARNLHKTAVH NGAGGALFVH RDTPENNPDT PFDFTPENYK RIEAIVKNYP EGHQAAAVLP VLDLAQRQN GWLPISAMNK VAEVLQVPPM RVYEVATFYT MYNRKPVGKY HIQVCTTTPC MLRDSDSILE TLQRKLGIKV G ETTPDKLF TLIEVECLGA CVNAPMVQIN DNYYEDLTPK DIEEIIDELK AGKVPKPGPR SGRFCCEPAG GLTSLTEPPK GP GFGVQ

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

分子 #6: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial; タイプ: protein_or_peptide / ID: 6 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 50.904152 KDa

配列

文字列:

MLAARHFLGG LVPVRVSVRF SSGTTAPKKT SFGSLKDEDR IFTNLYGRHD WRLKGALRRG DWYKTKEILL KGPDWILGEM KTSGLRGRG GAGFPTGLKW SFMNKPSDGR PKYLVVNADE GEPGTCKDRE IMRHDPHKLV EGCLVGGRAM GARAAYIYIR G EFYNEASN LQVAIREAYE AGLIGKNACG SDYDFDVFVV RGAGAYICGE ETALIESIEG KQGKPRLKPP FPADVGVFGC PT TVANVET VAVSPTICRR GGTWFAGFGR ERNSGTKLFN ISGHVNHPCT VEEEMSVPLK ELIEKHAGGV TGGWDNLLAV IPG GSSTPL IPKSVCETVL MDFDALVQAQ TGLGTAAVIV MDRSTDIVKA IARLIEFYKH ESCGQCTPCR EGVDWMNKVM ARFV KGDAR PAEIDSLWEI SKQIEGHTIC ALGDGAAWPV QGLIRHFRPE LEDRMQRFAQ QHRAWQAAS

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

分子 #7: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

• 分子: 名称: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; タイプ: protein_or_peptide / ID: 7 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 78.652367 KDa

配列

文字列:

MLRIPIKRAL IGLSNSPKGY VRTTGTAASN LIEVFVDGQS VMVEPGTTVL QACEKVGMQI PRFCYHERLS VAGNCRMCLV EIEKAPKVV AACAMPVMKG WNILTNSEKS KKAREGVMEF LLANHPLDCP ICDQGGECDL QDQSMMFGSD RSRFLEGKRA V EDKNIGPL VKTIMTRCIQ CTRCIRFASE IAGVDDLGTT GRGNDMQVGT YIEKMFMSEL SGNVIDICPV GALTSKPYAF TA RPWETRK TESIDVMDAV GSNIVVSTRT GEVMRILPRM HEDINEEWIS DKTRFAYDGL KRQRLTEPMV RNEKGLLTYT SWE DALSRV AGMLQNFEGN AVAAIAGGLV DAEALVALKD LLNKVDSDNL CTEEIFPTEG AGTDLRSNYL LNTTIAGVEE ADVV LLVGT NPRFEAPLFN ARIRKSWLHN DLKVALIGSP VDLTYRYDHL GDSPKILQDI ASGRHSFCEV LKDAKKPMVV LGSSA LQRD DGAAILVAVS NMVQKIRVTT GVAAEWKVMN ILHRIASQVA ALDLGYKPGV EAIRKNPPKM LFLLGADGGC ITRQDL PKD CFIVYQGHHG DVGAPMADVI LPGAAYTEKS ATYVNTEGRA QQTKVAVTPP GLAREDWKII RALSEIAGIT LPYDTLD QV RNRLEEVSPN LVRYDDIEET NYFQQASELA KLVNQEVLAD PLVPPQLTIK DFYMTDSISR ASQTMAKCVK AVT

UniProtKB: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

分子 #8: NADH-ubiquinone oxidoreductase chain 1

• 分子: 名称: NADH-ubiquinone oxidoreductase chain 1 / タイプ: protein_or_peptide / ID: 8 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 36.105027 KDa

配列

文字列:

(FME)FFINILTLL VPILIAMAFL TLVERKILGY MQLRKGPNIV GPYGILQPFA DAMKLFMKEP MRPLTTSMSL FIIAPT LSL TLALSLWVPL PMPHPLINLN LGILFILATS SLSVYSILWS GWASNSKYSL FGALRAVAQT ISYEVTMAII LLSVLLM NG SYSLQTLITT QEHMWLLLPA WPMAMMWFIS TLAETNRAPF DLTEGESELV SGFNVEYAAG PFALFFMAEY TNIILMNA L TTIIFLGPLY YINLPELYST NFMMEALLLS STFLWIRASY PRFRYDQLMH LLWKNFLPLT LALCMWHISL PIFTAGVPP YM

UniProtKB: NADH-ubiquinone oxidoreductase chain 1

分子 #9: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial; タイプ: protein_or_peptide / ID: 9 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 24.068355 KDa

配列

文字列:

MYRLSSSMLP RALAQAMRTG HLNGQSLHSS AVAATYKYVN KKEQESEVDM KSATDNAARI LMWTELIRGL GMTLSYLFRE PATINYPFE KGPLSPRFRG EHALRRYPSG EERCIACKLC EAICPAQAIT IEAEPRADGS RRTTRYDIDM TKCIYCGFCQ E ACPVDAIV EGPNFEFSTE THEELLYNKE KLLNNGDKWE AEIAANIQAD YLYR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

分子 #10: NADH-ubiquinone oxidoreductase chain 6

• 分子: 名称: NADH-ubiquinone oxidoreductase chain 6 / タイプ: protein_or_peptide / ID: 10 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 18.6561 KDa

配列

文字列:

(FME)NNYIFVLSS LFLVGCLGLA LKPSPIYGGL GLIVSGFVGC LMVLGFGGSF LGLMVFLIYL GGMLVVFGYT TAMATE EYP ETWGSNWLIL GFLVLGVIME VFLICVLNYY DEVGVINLDG LGDWLMYEVD DVGVMLEGGI GVAAMYSCAT WMMVVAG WS LFAGIFIIIE ITRD

UniProtKB: NADH-ubiquinone oxidoreductase chain 6

分子 #11: NADH-ubiquinone oxidoreductase chain 4L

• 分子: 名称: NADH-ubiquinone oxidoreductase chain 4L / タイプ: protein_or_peptide / ID: 11 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 10.637629 KDa

配列

文字列:

(FME)PSTFFNLTM AFSLSLLGTL MFRSHLMSTL LCLEGMVLSL FIMTSVTSLN SNSMSSMPIP ITILVFAACE AAVGLA LLV KVSNTYGTDY VQNLNLLQC

UniProtKB: NADH-ubiquinone oxidoreductase chain 4L

分子 #12: NADH-ubiquinone oxidoreductase chain 5

• 分子: 名称: NADH-ubiquinone oxidoreductase chain 5 / タイプ: protein_or_peptide / ID: 12 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 68.547297 KDa

配列

文字列:

(FME)NIFTTSILL IFILLLSPIL ISMSNLIKHI NFPLYTTTSI KFSFIISLLP LLMFFHNNME YMITTWHWVT MNSMEL KMS FKTDFFSILF TSVALFVTWS IMQFSSWYMH SDPNINRFIK YLTLFLITML ILTSANNMFQ LFIGWEGVGI MSFLLIG WW YGRTDANTAA LQAILYNRIG DIGFILAMVW FSLNMNSWEL QQIMFSNNND NLIPLMGLLI AATGKSAQFG LHPWLPSA M EGPTPVSALL HSSTMVVAGI FLLVRFHPLT TNNNFILTTM LCLGALTTLF TAICALTQND IKKIIAFSTS SQLGLMMVT LGMNQPHLAF LHICTHAFFK AMLFMCSGSI IHSLADEQDI RKMGNITKIM PFTSSCLVIG SLALTGMPFL TGFYSKDLII EAINTCNTN AWALLITLIA TSMTAMYSMR IIYFVTMTKP RFPPLISINE NDPDLMNPIK RLAFGSIFAG FVISYNIPPT S IPVLTMPW FLKTTALIIS VLGFLIALEL NNLTMKLSMN KANPYSSFST LLGFFPSIIH RITPMKSLNL SLKTSLTLLD LI WLEKTIP KSTSTLHTNM TTLTTNQKGL IKLYFMSFLI NIILIIILYS INLE

UniProtKB: NADH-ubiquinone oxidoreductase chain 5

分子 #13: NADH-ubiquinone oxidoreductase chain 4

• 分子: 名称: NADH-ubiquinone oxidoreductase chain 4 / タイプ: protein_or_peptide / ID: 13 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 51.943547 KDa

配列

文字列:

(FME)LKIILPSLM LLPLTWLSSP KKTWTNVTSY SFLISLTSLT LLWQTDENYK NFSNMFSSDP LSTPLIILTA WLLPLM LMA SQNHLKKDNN VLQKLYISML ISLQILLIMT FSATELIMFY ILFEATLIPT LIIITRWGNQ TERLNAGIYF LFYTLIG SI PLLIALILIQ NHVGTLNLMI LSFTTHTLDA SWSNNLLWLA CMMAFLIKMP LYGVHLWLPK AHVEAPIAGS MILAAILL K LGSYGMIRIS IILDPLTKYM AYPFILLSLW GMIMTSSICL RQTDLKSLIA YSSVSHMALV IASIMIQTPW SFMGATMLM IAHGLTSSLL FCLANSNYER IHSRTMIMAR GLQMVFPLMA TWWLMASLAN LALPPSINLM GELFITMSLF SWSNFTIILM GINIIITGM YSMYMIITTQ RGKLTNHMIN LQPSHTRELT LMALHMIPLI LLTTSPKLIT GLTM

UniProtKB: NADH-ubiquinone oxidoreductase chain 4

分子 #14: NADH-ubiquinone oxidoreductase chain 2

• 分子: 名称: NADH-ubiquinone oxidoreductase chain 2 / タイプ: protein_or_peptide / ID: 14 / コピー数: 1 / 光学異性体: LEVO / EC番号: NADH:ubiquinone reductase (H+-translocating)
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 38.80023 KDa

配列

文字列:

(FME)NPITLAIIY FTIFLGPVIT MSSTNLMLMW VGLEFSLLAI IPMLINKKNP RSTEAATKYF VTQATASMII LLAIVL NYK QLGTWMFQQQ TNGLILNMTL MALSMKLGLA PFHFWLPEVT QGIPLHMGLI LLTWQKIAPL SILIQIYPLL NSTIILM LA ITSIFMGAWG GLNQTQMRKI MAYSSIAHMG WMLAILPYNP SLTLLNLMIY IILTAPMFMA LMLNNSMTIN SISLLWNK T PAMLTMISLM LLSLGGLPPL TGFLPKWIII TELMKNNCLI MATLMAMMAL LNLFFYTRLI YSTSLTMFPT NNNSKMMTH QTKTKPNLMF STLAIMSTMT LPLAPQLIT

UniProtKB: NADH-ubiquinone oxidoreductase chain 2

分子 #15: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mi...

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; タイプ: protein_or_peptide / ID: 15 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 40.657375 KDa

配列

文字列:

MALRLLRLVP ASAPARGLAA GAQRVGRIHT SVHCKLRYGL LAAILGDKTT KKLHEYSRVI TVDGNICSGK NKLAKEIAQQ LGMKHYPEA GIQYSSTTTG DGRPLDIEFS GSCSLEKFYD DPKSNDGNSY RLQSWLYASR LLQYADALEH LLSTGQGVVL E RSIYSDFV FLEAMYNQGY IRKQCVDHYN EIKRLTLPEY LPPHAVIYID VPVPEVQSRI QKKGDPHEMK VTSAYLQDIE NA YKKTFLP KMSEMCEVLV YDSWEAEDPT KVVEDIEYLK YNKGPWLKQD DWTFHYLRML VQDKTEVLNY TTIPVYLPEI TIG AHQGSR IYNSFRELPG RKYAPGYNAE VGDKWIWLK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial

分子 #16: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mit...

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial; タイプ: protein_or_peptide / ID: 16 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 42.588129 KDa

配列

文字列:

MAAAVRFRVV RALPMSRPAI TAAATSVFCG SSHRQLHHAV IPHGKGGRSS VSGVVATVFG ATGFLGRYVV NHLGRMGSQV IIPYRCDVY DIMHLRLMGD LGQLTFLEWD ARDKDSIRKA VQHSNVVINL IGREWETRNF DFEDVFVNIP RAIAQASKEA G VERFIHVS HLNASMKSSS KSLRSKAVGE KEVRSVFPEA IIIRPSDIFG REDRFLNHFA NYRWFLAVPL VSLGFKTVKQ PV YVADVSK GIVNATKDPD AVGKTFAFTG PNRYLLFHLV KYIFGMTHRT FIPYPLPLFV YSWIGKLFGL SPFEPWTTKD KVE RIHISD VMPTDLPGLE DLGVQPTPLE LKSIEVLRRH RTYRWLSSEI EETKPAKTVN Y

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial

分子 #17: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial; タイプ: protein_or_peptide / ID: 17 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 19.814725 KDa

配列

文字列:

MAAVSISVSL RQAMLGRRAM ATAAVSVCRV PSRLLSTSTW KLADNQTRDT QLITVDEKLD ITTLTGVPEE HIKTRKVRIF VPARNNMQS GVNNTKKWKM EFDTRERWEN PLMGWASTAD PLSNMVLTFS AKEDAIAFAE KNGWSYDVEE KKVPKPKSKS Y GANFSWNK RTRVSTK

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

分子 #18: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial; タイプ: protein_or_peptide / ID: 18 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 12.765534 KDa

配列

文字列:

MAAVLTFRRL LTLPRAARGF GVQVSPSGEK ITHTGQVYDE KDYRRVRFVD RQKEVNENFA IDLIAQQPVN EVEHRIIACD GGGGALGHP KVYINLDKET KTGTCGYCGL QFKQH

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

分子 #19: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; タイプ: protein_or_peptide / ID: 19 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 10.932675 KDa

配列

文字列:

MAAAAASRAV GAKLGLREIR VHLCQRSPGS QGVRDFIVQR YVELKKAHPN LPILIRECSE VQPKLWARYA FGQEKTVSLN NLSADEVTR AMQNVLSGKA

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

分子 #20: Acyl carrier protein, mitochondrial

• 分子: 名称: Acyl carrier protein, mitochondrial / タイプ: protein_or_peptide / ID: 20 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 17.390289 KDa

配列

文字列:

MASRVLCACV RRLPAAFAPL PRLPTLALAR PLSTTLCPEG IRRRPGALQS ALALAQVPGT VTHLCRQYSD APPLTLDGIK DRVLYVLKL YDKIDPEKLS VNSHFMKDLG LDSLDQVEII MAMEDEFGFE IPDIDAEKLM CPQEIVDYIA DKKDVYE

UniProtKB: Acyl carrier protein, mitochondrial

分子 #21: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5; タイプ: protein_or_peptide / ID: 21 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 13.380719 KDa

配列

文字列:

MAGLLKKTTG LVGLAVCDTP HERLTILYTK TLDILKHFPK HAAYRKYTEQ ITNEKLDMVK AEPDVKKLEA LLQGGEVEEV ILQAEKELS LARKMLKWKP WEPLVEEPPA NQWKWPI

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

分子 #22: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6; タイプ: protein_or_peptide / ID: 22 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 15.311858 KDa

配列

文字列:

MAAAATGLRQ AAAAAASTSV KPIFSRDLNE AKRRVRELYR AWYREVPNTV HLMQLDITVK QGRDKVREMF MKNAHVTDPR VVDLLVIKG KMELQETIKV WKQRTHVMRF FHETETPRPK DFLSKFYMGH DP

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

分子 #23: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; タイプ: protein_or_peptide / ID: 23 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 20.025127 KDa

配列

文字列:

MPGIVELPTL EELKVEEVKV SSAVLKAAAH HYGAQCDKTN KEFMLCRWEE KDPRRCLKEG KLVNGCALNF FRQIKSHCAE PFTEYWTCL DYSNMQLFRH CRQQQAKFDQ CVLDKLGWVR PDLGQLSKVT KVKTDRPLPE NPYHSRARPE PNPVIEGDLK P AKHGTRFF FWTV

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

分子 #24: MCG5603

• 分子: 名称: MCG5603 / タイプ: protein_or_peptide / ID: 24 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 15.130416 KDa

配列

文字列:

MAMVKRFFES YHEVPDGTQC HRKTYITTAL GGICGIIGSA YSVSLNPADS TLEAVARVGR YTFTAAAIGA MFGLTTCVSA QVREKPDDP LNYFIGGCAG GLTLGARTHS YGTAAMGCVY MGTAAALFKI GKLEGWELFP TPKV

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11

分子 #25: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13; タイプ: protein_or_peptide / ID: 25 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 16.881588 KDa

配列

文字列:

MAASKVKQDM PPPGGYGPID YKRNLPRRGL SGYSMFAVGI GALIFGYWRM MRWNQERRRL LIEDLEARIA LMPLFQAEKD RRTLQILRE NLEEEAIIMK DVPNWKVGES VFHTTRWVPP LIGEMYGLRT KEEMSNANFG FTWYT

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

分子 #26: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; タイプ: protein_or_peptide / ID: 26 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 7.921279 KDa

配列

文字列:

MWFEILPGLA IMGVCLVIPG VSTAYIHKFT NGGKEKRVAR VQYQWYLMER DRRISGVNRY YVSKGLEN

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

分子 #27: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3; タイプ: protein_or_peptide / ID: 27 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 9.249709 KDa

配列

文字列:

(AYA)GRISAFLKN AWAKEPVLVV SFSVWGLAII MPMISPYTKY ASMINKATPY NYPVPVRDDG NMPDVPSHPQ DPLGPS LDW LKNL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

分子 #28: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial; タイプ: protein_or_peptide / ID: 28 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 8.636023 KDa

配列

文字列:

MAPSVVLRSF SRLLAPARLP SCSSTRSKFY VREPVNAKPN WLAVGLSVGA SVFMWIYLIQ THNEDVLEYK RRNGLE

UniProtKB: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial

分子 #29: NADH dehydrogenase [ubiquinone] 1 subunit C2

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 subunit C2 / タイプ: protein_or_peptide / ID: 29 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 14.185692 KDa

配列

文字列:

MMNGRPGHEP LKFLPDEARS LPPPKLNDPR LVYMGLLGYC TGLMDNMLRM RPVMRAGLHR QLLFVTSFVF AGYFYLKRQN YLYAVKDHD MFGYIKLHPE DFPEKEKKTY AEILEPFHPV R

UniProtKB: NADH dehydrogenase [ubiquinone] 1 subunit C2

分子 #30: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

• 分子: 名称: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5; タイプ: protein_or_peptide / ID: 30 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 12.675772 KDa

配列

文字列:

MPFLDIQKKL GISLDRHFMF LSAEQPYKNA ARCHAFEKEW IECAHGIGGT RAKKECKIEF DDFEECLLRY KTMRRMHDIK KQREKLMKE GKYTPPPHHS GREEPRP

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

分子 #31: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1; タイプ: protein_or_peptide / ID: 31 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 6.965109 KDa

配列

文字列:

MTLFQLLREH WVHILVPAGF VFGCYLDRKD DEKLTAFRNK SMLFQRELRP NEEVTWK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1

分子 #32: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mit...

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial; タイプ: protein_or_peptide / ID: 32 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 17.463727 KDa

配列

文字列:

MAARLLSLYG RCLSAAGAMR GLPAARVRWE SSRAVIAPSG VEKKRQREPT MQWQEDPEPE DENVYAKNPD FHGYDSDPVV DVWNMRAVF FFGFSIVLVF GTTFVAYVPD YRMQEWARRE AERLVKYREV NGLPIMESNY FDPSKIQLPE DD

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial

分子 #33: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mito...

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial; タイプ: protein_or_peptide / ID: 33 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 21.742197 KDa

配列

文字列:

MAAMSLLQRA SVSALTALSC RRAGPRLGVG SFLTRSFPKT VAPVRHSGDH GKRLFVVKPS LYYDARFLRL MKFYLMLTGI PVIIGITLV NIFIGEAELA EIPEGYIPEH WEYYKHPISR WIARNFYDGP EKNYEKTLAI LQIESEKAEL RLKEQEVRRL M RARGDGPW YQFPTPEKEF IDHSPKATPD N

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial

分子 #34: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6; タイプ: protein_or_peptide / ID: 34 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 15.450925 KDa

配列

文字列:

(SAC)GYTPDEKLR LQQLRELRRR WLKDQELSPR EPVLPPRRMW PLERFWDNFL RDGAVWKNMV FKAYRSSLFA VSHVLI PMW FVHYYVKYHM ATKPYTIVSS KPRIFPGDTI LETGEVIPPM RDFPDQHH

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6

分子 #35: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mito...

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial; タイプ: protein_or_peptide / ID: 35 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 11.982437 KDa

配列

文字列:

MSALTRLVPF GRVGGRLLRG CRARAAGDSG VRHAGGGVHI QPRYREFPQL TRSQVIQGEF LSSLMWFWIL WRFWHDSDAV LGHFSYPDP SQWTDEELGI PPDDED

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial

分子 #36: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3; タイプ: protein_or_peptide / ID: 36 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 11.71424 KDa

配列

文字列:

MAAGHGHEHG HEHGHGHGKM ELPDYRQWKI EGTPLETVQK KLAARGLRDP WARNEAWRYM GGFAGNITFP SVILKGFKWG FAAFVVALG AEYFLDSQNG DKKHH

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3

分子 #37: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mito...

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; タイプ: protein_or_peptide / ID: 37 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 21.903828 KDa

配列

文字列:

MAAARAAALG VRWLQRTTRG VVPLEARRAF HMTKDMLPGS YPRTPEERAA AAKKYNMRVE DYEPYPDDGM GYGDYPMLPN RSQHERDPW YQWDHSELRM NWGEPIHWDL DMYIRNRVDT SPTPVSWDVM CKHLFGFVAF MVFMFWVGHV FPSYQPVGPK Q YPYNNLYL ERGGDPTKEP EPVVHYDI

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial

分子 #38: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4; タイプ: protein_or_peptide / ID: 38 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 15.105287 KDa

配列

文字列:

MSGSKYKPAP LATLPSTLDP AEYDVSPETR RAQVERLSIR ARLKREYLLQ YNDPKRVSHI EDPALIRWTY ARSANIYPNF RPTPKNSLL GAVAGFGPLI FWYYVFKTDR DRKERLIQEG KLDRKFNISY

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

分子 #39: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; タイプ: protein_or_peptide / ID: 39 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 22.020123 KDa

配列

文字列:

MAFCAPPAYL THQQKVLRLY KRALRHLESW CIHRDKYRYF ACLMRARFEE HKNEKDMMRA TQLLREAEEE FWQNQHPQPY IFPDSPGGT SFERYECYKV PEWCLDYWHP SEKAMYPDYF SKREQWKKLR MESWDREVKQ LQEETSPDGI MTEALPPARR E GDLPPLWW HIVTRPRERP T

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

分子 #40: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7; タイプ: protein_or_peptide / ID: 40 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 16.360804 KDa

配列

文字列:

MGAHLTRRYL WDASVEPDPE KIPSFPPDLG FPERKERVMV ATQQEMMDAQ LTLQQRDYCA HYLIRLLKCK RDSFPNFLAC KHEQHDWDY CEHLDYVKRM KEFERERRLL QRKKRRALKE ARVAQGQGEG EVGPEVAL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

分子 #41: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10; タイプ: protein_or_peptide / ID: 41 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 21.054832 KDa

配列

文字列:

MPDSWDKDVY PEPPSRTPAP SPQTSLPNPI TYLTKAYDLV VDWPVTLVRE FIERQHAKNR TYYYHRQYRR VPDITECKEG DVLCIYEAE MQWRRDFKVD QEIMNIIQER LKACQQREGE NYQQNCAKEL EQFTKVTKAY QDRYLDLGAY YSARKCLAKQ K QRMLEERK AARQEAAA

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

分子 #42: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12; タイプ: protein_or_peptide / ID: 42 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 17.112416 KDa

配列

文字列:

MELVEVLKRG VQQVTGHGGL RGLLRVFFRA NDIRIGTLVG EDKYGNKYYE DNKQFFGRHR WVIYTTEMNG KNTFWDVDGS MVPPEWHRW LHCMTDDPPT TNPPTARKFI WTNHKFNVSA TPEQYVPYST TRKKIHEWVP PSTPYK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

分子 #43: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

• 分子: 名称: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7; タイプ: protein_or_peptide / ID: 43 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 12.506432 KDa

配列

文字列:

(AYA)SATRVIQKL RNWASGQDLQ AKLQLRYQEI AKRTQPPPKL PVGPSHKLSN NYYCTRDGRR EVVPPSIIMS SQKALV SGK AAESSAMAAT EKKAVTPAPP MKRWELSKDQ PYL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

分子 #44: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial

• 分子: 名称: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial; タイプ: protein_or_peptide / ID: 44 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Mus musculus (ハツカネズミ) / 組織: heart
• 分子量: 理論値: 11.833504 KDa

配列

文字列:

MAVSLLLRGG RIRALKAVLL EARVFPGELV SVVRLSTESE KSAKEKELHP KTQSVLKEPE PTDTTTYKNL QHHDYNTYTF LDLNLDLSK FRLPQPSSGR ESPRH

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial

分子 #45: IRON/SULFUR CLUSTER

• 分子: 名称: IRON/SULFUR CLUSTER / タイプ: ligand / ID: 45 / コピー数: 6 / 式: SF4
• 分子量: 理論値: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / 一硫化鉄

分子 #46: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE

• 分子: 名称: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / タイプ: ligand / ID: 46 / コピー数: 2 / 式: PC1
• 分子量: 理論値: 790.145 Da

Chemical component information

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質*YM

分子 #47: FE2/S2 (INORGANIC) CLUSTER

• 分子: 名称: FE2/S2 (INORGANIC) CLUSTER / タイプ: ligand / ID: 47 / コピー数: 2 / 式: FES
• 分子量: 理論値: 175.82 Da

Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

分子 #48: FLAVIN MONONUCLEOTIDE

• 分子: 名称: FLAVIN MONONUCLEOTIDE / タイプ: ligand / ID: 48 / コピー数: 1 / 式: FMN
• 分子量: 理論値: 456.344 Da

Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE / FMN

分子 #49: 1,2-Distearoyl-sn-glycerophosphoethanolamine

• 分子: 名称: 1,2-Distearoyl-sn-glycerophosphoethanolamine / タイプ: ligand / ID: 49 / コピー数: 8 / 式: 3PE
• 分子量: 理論値: 748.065 Da

Chemical component information

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / DSPE / リン脂質*YM

分子 #50: CARDIOLIPIN

• 分子: 名称: CARDIOLIPIN / タイプ: ligand / ID: 50 / コピー数: 4 / 式: CDL
• 分子量: 理論値: 1.464043 KDa

Chemical component information

ChemComp-CDL:
CARDIOLIPIN / リン脂質*YM

分子 #51: ADENOSINE-5'-TRIPHOSPHATE

• 分子: 名称: ADENOSINE-5'-TRIPHOSPHATE / タイプ: ligand / ID: 51 / コピー数: 1 / 式: ATP
• 分子量: 理論値: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子*YM

分子 #52: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

• 分子: 名称: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE; タイプ: ligand / ID: 52 / コピー数: 1 / 式: NDP
• 分子量: 理論値: 745.421 Da

Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / NADPH

分子 #53: ZINC ION

• 分子: 名称: ZINC ION / タイプ: ligand / ID: 53 / コピー数: 1 / 式: ZN
• 分子量: 理論値: 65.409 Da

分子 #54: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butan...

• 分子: 名称: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate; タイプ: ligand / ID: 54 / コピー数: 2 / 式: EHZ
• 分子量: 理論値: 584.703 Da

Chemical component information

ChemComp-EHZ:
~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate

実験情報

構造解析

• 手法: クライオ電子顕微鏡法
• 解析: 単粒子再構成法
• 試料の集合状態: particle

試料調製

• 緩衝液: pH: 7.4
• グリッド: モデル: UltrAuFoil R0.6/1 / 材質: GOLD
• 凍結: 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277.15 K / 装置: FEI VITROBOT MARK IV / 詳細: 8-16s blotting.

電子顕微鏡法

• 顕微鏡: FEI TITAN KRIOS
• 撮影: フィルム・検出器のモデル: GATAN K2 QUANTUM (4k x 4k); 検出モード: COUNTING / 平均電子線量: 50.0 e/Ų
• 電子線: 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN
• 電子光学系: 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm
• 実験機器: モデル: Titan Krios / 画像提供: FEI Company

画像解析

初期モデル

モデルのタイプ: PDB ENTRY
PDBモデル - PDB ID:

6g72

• 最終 再構成: 解像度のタイプ: BY AUTHOR / 解像度: 3.17 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 50184
• 初期 角度割当: タイプ: MAXIMUM LIKELIHOOD
• 最終 角度割当: タイプ: MAXIMUM LIKELIHOOD