ジャーナル: Sci Adv / 年: 2021 タイトル: Trans-synaptic assemblies link synaptic vesicles and neuroreceptors. 著者: Antonio Martinez-Sanchez / Ulrike Laugks / Zdravko Kochovski / Christos Papantoniou / Luca Zinzula / Wolfgang Baumeister / Vladan Lučić / 要旨: Synaptic transmission is characterized by fast, tightly coupled processes and complex signaling pathways that require a precise protein organization, such as the previously reported nanodomain ...Synaptic transmission is characterized by fast, tightly coupled processes and complex signaling pathways that require a precise protein organization, such as the previously reported nanodomain colocalization of pre- and postsynaptic proteins. Here, we used cryo-electron tomography to visualize synaptic complexes together with their native environment comprising interacting proteins and lipids on a 2- to 4-nm scale. Using template-free detection and classification, we showed that tripartite trans-synaptic assemblies (subcolumns) link synaptic vesicles to postsynaptic receptors and established that a particular displacement between directly interacting complexes characterizes subcolumns. Furthermore, we obtained de novo average structures of ionotropic glutamate receptors in their physiological composition, embedded in plasma membrane. These data support the hypothesis that synaptic function is carried by precisely organized trans-synaptic units. It provides a framework for further exploration of synaptic and other large molecular assemblies that link different cells or cellular regions and may require weak or transient interactions to exert their function.
名称: Native putative NMDA-type ionotropic glutamate receptor imaged at excitatory neocortical rodent synapses タイプ: complex / ID: 1 / 親要素: 0 詳細: Postsynaptic complexes were detected by Morse density tracing and classified by Affinity propagation (both implemented in PySeg) to obtain putative NMDA receptor particles. By using PDB-6MMP ...詳細: Postsynaptic complexes were detected by Morse density tracing and classified by Affinity propagation (both implemented in PySeg) to obtain putative NMDA receptor particles. By using PDB-6MMP as reference model, the structure ware further refined and classified in 3D with Relion.