ジャーナル: Front Microbiol / 年: 2020 タイトル: Cryo-Electron Tomography Reveals the Complex Ultrastructural Organization of Multicellular Filamentous () Bacteria. 著者: Vasil A Gaisin / Romain Kooger / Denis S Grouzdev / Vladimir M Gorlenko / Martin Pilhofer / 要旨: The cell biology of is poorly studied. We applied cryo-focused ion beam milling and cryo-electron tomography to study the ultrastructural organization of thermophilic and , and mesophilic ". ...The cell biology of is poorly studied. We applied cryo-focused ion beam milling and cryo-electron tomography to study the ultrastructural organization of thermophilic and , and mesophilic ". Viridilinea mediisalina." These species represent the three main lineages within a group of multicellular filamentous anoxygenic phototrophic bacteria belonging to the order. We found surprising structural complexity in the . As with filamentous cyanobacteria, cells of and ". Viridilinea mediisalina" share the outer membrane-like layers of their intricate multilayer cell envelope. Additionally, cells of and ". Viridilinea mediisalina" are connected by septal channels that resemble cyanobacterial septal junctions. All three strains possess long pili anchored close to cell-to-cell junctions, a morphological feature comparable to that observed in cyanobacteria. The cytoplasm of the bacteria is crowded with intracellular organelles such as different types of storage granules, membrane vesicles, chlorosomes, gas vesicles, chemoreceptor-like arrays, and cytoplasmic filaments. We observed a higher level of complexity in the mesophilic strain compared to the thermophilic strains with regards to the composition of intracellular bodies and the organization of the cell envelope. The ultrastructural details that we describe in these bacteria will motivate further cell biological studies, given that the function and evolution of the many discovered morphological traits remain enigmatic in this diverse and widespread bacterial group.
集束イオンビーム - 装置: OTHER / 集束イオンビーム - イオン: OTHER / 集束イオンビーム - 電圧: 5 kV / 集束イオンビーム - 電流: 0.035 nA / 集束イオンビーム - 時間: 300 sec. / 集束イオンビーム - 温度: 125 K / 集束イオンビーム - Initial thickness: 1000 nm / 集束イオンビーム - 最終 厚さ: 300 nm 集束イオンビーム - 詳細: The value given for _emd_sectioning_focused_ion_beam.instrument is Helios. This is not in a list of allowed values set(['DB235', 'OTHER']) so OTHER is written into the XML file.