EMDB-10537: Yeast 80S ribosome in complex with eIF5A and decoding A-site and ...

Basic information

• Entry: Database: EMDB / ID: EMD-10537
• Title: Yeast 80S ribosome in complex with eIF5A and decoding A-site and P-site tRNAs.
• Map data: Cryo-EM density of the yeast 80S ribosome in complex with eIF5A and A-site and P-site tRNAs. Filtered according to local resolution.

Sample

• Complex: Yeast 80S ribosome in complex with eIF5A programed by A-site and P-site tRNA.
  • RNA: x 7 types
  • Protein or peptide: x 76 types
• Ligand: x 3 types

• Keywords: eIF5A / translation control / TRANSLATION

Function / homology

Function:

cytoplasmic translational elongation through polyproline stretches / positive regulation of cytoplasmic translational elongation through polyproline stretches / Hypusine synthesis from eIF5A-lysine / CAT tailing / translational frameshifting / cytoplasmic translational elongation / positive regulation of translational termination / cytoplasmic translational termination / positive regulation of translational elongation / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / regulation of amino acid metabolic process / negative regulation of glucose mediated signaling pathway / positive regulation of translational fidelity / RMTs methylate histone arginines / Protein methylation / mTORC1-mediated signalling / Protein hydroxylation / ribosome-associated ubiquitin-dependent protein catabolic process / GDP-dissociation inhibitor activity / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / pre-mRNA 5'-splice site binding / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / preribosome, small subunit precursor / nonfunctional rRNA decay / response to cycloheximide / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Major pathway of rRNA processing in the nucleolus and cytosol / mRNA destabilization / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / negative regulation of translational frameshifting / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / Formation of a pool of free 40S subunits / preribosome, large subunit precursor / positive regulation of protein kinase activity / L13a-mediated translational silencing of Ceruloplasmin expression / positive regulation of translational initiation / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit export from nucleus / translational elongation / G-protein alpha-subunit binding / 90S preribosome / translation elongation factor activity / ribosomal subunit export from nucleus / regulation of translational fidelity / protein-RNA complex assembly / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translational termination / maturation of LSU-rRNA / ribosomal small subunit export from nucleus / translation regulator activity / DNA-(apurinic or apyrimidinic site) endonuclease activity / translation initiation factor activity / rescue of stalled ribosome / cellular response to amino acid starvation / protein kinase C binding / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosome assembly / maturation of SSU-rRNA / small-subunit processome / translational initiation / macroautophagy / maintenance of translational fidelity / modification-dependent protein catabolic process / protein tag activity / cytoplasmic stress granule / rRNA processing / ribosome biogenesis / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / small ribosomal subunit rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / protein ubiquitination / structural constituent of ribosome / ribosome / translation / G protein-coupled receptor signaling pathway / ribonucleoprotein complex / negative regulation of gene expression / response to antibiotic / mRNA binding / ubiquitin protein ligase binding / nucleolus / perinuclear region of cytoplasm / mitochondrion

Domain/homology:

Translation elongation factor, IF5A, hypusine site / Eukaryotic initiation factor 5A hypusine signature. / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold / : / Translation initiation factor 5A-like, N-terminal / Translation elongation factor, IF5A C-terminal / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold / Translation elongation factor IF5A-like / : / : / Ribosomal protein S26e signature. / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / : / Ribosomal protein S12e signature. / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S12e / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein S5, eukaryotic/archaeal / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / S27a-like superfamily / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / 40S Ribosomal protein S10 / : / Ribosomal protein S7e signature. / Ribosomal protein L19, eukaryotic / Ribosomal protein L10e / Ribosomal protein L24e, conserved site / : / Ribosomal protein L24e signature. / Ribosomal protein L44e signature. / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L6e signature. / Ribosomal protein L13e / Ribosomal protein L13e / Plectin/S10, N-terminal / Plectin/S10 domain / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S30 / Ribosomal protein S17e, conserved site / Ribosomal protein S30 / Ribosomal protein S17e signature. / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein S3Ae, conserved site / Ribosomal protein S3Ae signature. / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / : / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L30e signature 1. / Ribosomal L40e family / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein 60S L18 and 50S L18e / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein L36e signature. / Ribosomal protein L35Ae, conserved site / : / Ribosomal protein L35Ae signature. / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein S27e signature. / Ribosomal protein S3, eukaryotic/archaeal

Component:

40S ribosomal protein S8 / Rps5p / Small ribosomal subunit protein uS2A / Small ribosomal subunit protein eS1A / Small ribosomal subunit protein uS4A / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL24A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein eL6B / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL15A / Large ribosomal subunit protein eL22A / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS15 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein eS19A / Small ribosomal subunit protein eS21A / Small ribosomal subunit protein uS8A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL31A / Ubiquitin-ribosomal protein eL40A fusion protein / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL42A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS30A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Large ribosomal subunit protein uL14A / Large ribosomal subunit protein uL2A / Small ribosomal subunit protein uS17A / Large ribosomal subunit protein eL18A / Small ribosomal subunit protein uS9A / Small ribosomal subunit protein uS13A / Large ribosomal subunit protein eL19A / Large ribosomal subunit protein uL29A / Large ribosomal subunit protein uL4A / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein eS17B / Large ribosomal subunit protein eL8A / Eukaryotic translation initiation factor 5A-1 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL13A / Small ribosomal subunit protein eS7A / Small ribosomal subunit protein eS27A / Large ribosomal subunit protein eL14A / Small ribosomal subunit protein RACK1 / Large ribosomal subunit protein eL32 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS11B / Small ribosomal subunit protein eS26B / Small ribosomal subunit protein uS14A / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein eS12 / Large ribosomal subunit protein eL37A / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein eL34A / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein eL21A / Small ribosomal subunit protein eS10A / Large ribosomal subunit protein eL13A / Large ribosomal subunit protein uL5B / Small ribosomal subunit protein eS25A / Small ribosomal subunit protein eS28A

• Biological species: Saccharomyces cerevisiae (brewer's yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 3.1 Å
• Authors: Buschauer R / Cheng J

Funding support

Germany, 1 items

Organization	Grant number	Country
German Research Foundation (DFG)	BE1814/15-1	Germany

• Citation: Journal: Science / Year: 2020; Title: The Ccr4-Not complex monitors the translating ribosome for codon optimality.; Authors: Robert Buschauer / Yoshitaka Matsuo / Takato Sugiyama / Ying-Hsin Chen / Najwa Alhusaini / Thomas Sweet / Ken Ikeuchi / Jingdong Cheng / Yasuko Matsuki / Risa Nobuta / Andrea Gilmozzi / Otto Berninghausen / Petr Tesina / Thomas Becker / Jeff Coller / Toshifumi Inada / Roland Beckmann / ; Abstract: Control of messenger RNA (mRNA) decay rate is intimately connected to translation elongation, but the spatial coordination of these events is poorly understood. The Ccr4-Not complex initiates mRNA decay through deadenylation and activation of decapping. We used a combination of cryo-electron microscopy, ribosome profiling, and mRNA stability assays to examine the recruitment of Ccr4-Not to the ribosome via specific interaction of the Not5 subunit with the ribosomal E-site in This interaction occurred when the ribosome lacked accommodated A-site transfer RNA, indicative of low codon optimality. Loss of the interaction resulted in the inability of the mRNA degradation machinery to sense codon optimality. Our findings elucidate a physical link between the Ccr4-Not complex and the ribosome and provide mechanistic insight into the coupling of decoding efficiency with mRNA stability.

History

Deposition	Dec 10, 2019	-
Header (metadata) release	Apr 22, 2020	-
Map release	Apr 22, 2020	-
Update	Nov 6, 2024	-
Current status	Nov 6, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_10537.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Cryo-EM density of the yeast 80S ribosome in complex with eIF5A and A-site and P-site tRNAs. Filtered according to local resolution.
• Voxel size: X=Y=Z: 1.084 Å

Density

Contour Level	By AUTHOR: 0.035 / Movie #1: 0.035
Minimum - Maximum	-0.15684655 - 0.37438583
Average (Standard dev.)	0.000019606867 (±0.0101471385)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 480 480 480 Spacing 480 480 480
Cell	A=B=C: 520.32 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.084	1.084	1.084
M x/y/z	480	480	480
origin x/y/z	0.000	0.000	0.000
length x/y/z	520.320	520.320	520.320
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	480	480	480
D min/max/mean	-0.157	0.374	0.000

Supplemental data

Sample components

Entire : Yeast 80S ribosome in complex with eIF5A programed by A-site and ...

• Entire: Name: Yeast 80S ribosome in complex with eIF5A programed by A-site and P-site tRNA.

Components

• Complex: Yeast 80S ribosome in complex with eIF5A programed by A-site and P-site tRNA.
  • RNA: 18S rRNA
  • RNA: mRNA
  • Protein or peptide: 40S ribosomal protein S0-A
  • Protein or peptide: 40S ribosomal protein S1-A
  • Protein or peptide: 40S ribosomal protein S15
  • Protein or peptide: 40S ribosomal protein S2
  • Protein or peptide: 40S ribosomal protein S3
  • Protein or peptide: 40S ribosomal protein S4-A
  • Protein or peptide: Rps5p
  • Protein or peptide: 40S ribosomal protein S6-A
  • Protein or peptide: 40S ribosomal protein S7-A
  • Protein or peptide: 40S ribosomal protein S8
  • Protein or peptide: 40S ribosomal protein S9-A
  • Protein or peptide: 40S ribosomal protein S10-A
  • Protein or peptide: 40S ribosomal protein S11-A
  • Protein or peptide: 40S ribosomal protein S12
  • Protein or peptide: 40S ribosomal protein S13
  • Protein or peptide: 40S ribosomal protein S14-B
  • Protein or peptide: 40S ribosomal protein S16-A
  • Protein or peptide: 40S ribosomal protein S17-B
  • Protein or peptide: 40S ribosomal protein S18-A
  • Protein or peptide: 40S ribosomal protein S19-A
  • Protein or peptide: 40S ribosomal protein S20
  • RNA: P-site tRNA
  • Protein or peptide: 40S ribosomal protein S21-A
  • Protein or peptide: 40S ribosomal protein S22-A
  • Protein or peptide: 40S ribosomal protein S23-A
  • Protein or peptide: 40S ribosomal protein S24-A
  • Protein or peptide: 40S ribosomal protein S25-A
  • Protein or peptide: 40S ribosomal protein S26-B
  • Protein or peptide: 40S ribosomal protein S27-A
  • Protein or peptide: 40S ribosomal protein S29-A
  • Protein or peptide: 40S ribosomal protein S30-A
  • Protein or peptide: Ubiquitin-40S ribosomal protein S31
  • Protein or peptide: Guanine nucleotide-binding protein subunit beta-like protein
  • Protein or peptide: 40S ribosomal protein S28-A
  • RNA: A-site tRNA
  • RNA: 25S rRNA
  • RNA: 5S rRNA
  • RNA: 5.8S rRNA
  • Protein or peptide: 60S ribosomal protein L2-A
  • Protein or peptide: 60S ribosomal protein L3
  • Protein or peptide: 60S ribosomal protein L4-A
  • Protein or peptide: 60S ribosomal protein L5
  • Protein or peptide: 60S ribosomal protein L6-B
  • Protein or peptide: 60S ribosomal protein L7-A
  • Protein or peptide: 60S ribosomal protein L8-A
  • Protein or peptide: 60S ribosomal protein L9-A
  • Protein or peptide: 60S ribosomal protein L10
  • Protein or peptide: 60S ribosomal protein L11-B
  • Protein or peptide: 60S ribosomal protein L13-A
  • Protein or peptide: 60S ribosomal protein L14-A
  • Protein or peptide: 60S ribosomal protein L15-A
  • Protein or peptide: 60S ribosomal protein L16-A
  • Protein or peptide: 60S ribosomal protein L17-A
  • Protein or peptide: 60S ribosomal protein L18-A
  • Protein or peptide: 60S ribosomal protein L19-A
  • Protein or peptide: 60S ribosomal protein L20-A
  • Protein or peptide: 60S ribosomal protein L21-A
  • Protein or peptide: 60S ribosomal protein L22-A
  • Protein or peptide: 60S ribosomal protein L23-A
  • Protein or peptide: 60S ribosomal protein L24-A
  • Protein or peptide: 60S ribosomal protein L25
  • Protein or peptide: 60S ribosomal protein L26-A
  • Protein or peptide: 60S ribosomal protein L27-A
  • Protein or peptide: 60S ribosomal protein L28
  • Protein or peptide: 60S ribosomal protein L29
  • Protein or peptide: 60S ribosomal protein L30
  • Protein or peptide: 60S ribosomal protein L31-A
  • Protein or peptide: 60S ribosomal protein L32
  • Protein or peptide: 60S ribosomal protein L33-A
  • Protein or peptide: 60S ribosomal protein L34-A
  • Protein or peptide: 60S ribosomal protein L35-A
  • Protein or peptide: 60S ribosomal protein L36-A
  • Protein or peptide: 60S ribosomal protein L37-A
  • Protein or peptide: 60S ribosomal protein L38
  • Protein or peptide: 60S ribosomal protein L39
  • Protein or peptide: Ubiquitin-60S ribosomal protein L40
  • Protein or peptide: 60S ribosomal protein L41-A
  • Protein or peptide: 60S ribosomal protein L42-A
  • Protein or peptide: 60S ribosomal protein L43-A
  • Protein or peptide: Eukaryotic translation initiation factor 5A-1
  • Protein or peptide: 60S ribosomal protein L1
• Ligand: ZINC ION
• Ligand: SPERMIDINE
• Ligand: 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione

Supramolecule #1: Yeast 80S ribosome in complex with eIF5A programed by A-site and ...

• Supramolecule: Name: Yeast 80S ribosome in complex with eIF5A programed by A-site and P-site tRNA.; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#83
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)

Macromolecule #1: 18S rRNA

• Macromolecule: Name: 18S rRNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 579.126562 KDa

Sequence

String:

UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGUAUAAC UGUGGUAAUU C UAGAGCUA AUACAUGCUU AAAAUCUCGA CCCUUUGGAA GAGAUGUAUU UAUUAGAUAA AAAAUCAAUG UCUUCGGACU CU UUGAUGA UUCAUAAUAA CUUUUCGAAU CGCAUGGCCU UGUGCUGGCG AUGGUUCAUU CAAAUUUCUG CCCUAUCAAC UUU CGAUGG UAGGAUAGUG GCCUACCAUG GUUUCAACGG GUAACGGGGA AUAAGGGUUC GAUUCCGGAG AGGGAGCCUG AGAA ACGGC UACCACAUCC AAGGAAGGCA GCAGGCGCGC AAAUUACCCA AUCCUAAUUC AGGGAGGUAG UGACAAUAAA UAACG AUAC AGGGCCCAUU CGGGUCUUGU AAUUGGAAUG AGUACAAUGU AAAUACCUUA ACGAGGAACA AUUGGAGGGC AAGUCU GGU GCCAGCAGCC GCGGUAAUUC CAGCUCCAAU AGCGUAUAUU AAAGUUGUUG CAGUUAAAAA GCUCGUAGUU GAACUUU GG GCCCGGUUGG CCGGUCCGAU UUUUCGUGUA CUGGAUUUCC AACGGGGCCU UUCCUUCUGG CUAACCUUGA GUCCUUGU G GCUCUUGGCG AACCAGGACU UUUACUUUGA AAAAAUUAGA GUGUUCAAAG CAGGCGUAUU GCUCGAAUAU AUUAGCAUG GAAUAAUAGA AUAGGACGUU UGGUUCUAUU UUGUUGGUUU CUAGGACCAU CGUAAUGAUU AAUAGGGACG GUCGGGGGCA UCAGUAUUC AAUUGUCAGA GGUGAAAUUC UUGGAUUUAU UGAAGACUAA CUACUGCGAA AGCAUUUGCC AAGGACGUUU U CAUUAAUC AAGAACGAAA GUUAGGGGAU CGAAGAUGAU CAGAUACCGU CGUAGUCUUA ACCAUAAACU AUGCCGACUA GG GAUCGGG UGGUGUUUUU UUAAUGACCC ACUCGGCACC UUACGAGAAA UCAAAGUCUU UGGGUUCUGG GGGGAGUAUG GUC GCAAGG CUGAAACUUA AAGGAAUUGA CGGAAGGGCA CCACCAGGAG UGGAGCCUGC GGCUUAAUUU GACUCAACAC GGGG AAACU CACCAGGUCC AGACACAAUA AGGAUUGACA GAUUGAGAGC UCUUUCUUGA UUUUGUGGGU GGUGGUGCAU GGCCG UUCU UAGUUGGUGG AGUGAUUUGU CUGCUUAAUU GCGAUAACGA ACGAGACCUU AACCUACUAA AUAGUGGUGC UAGCAU UUG CUGGUUAUCC ACUUCUUAGA GGGACUAUCG GUUUCAAGCC GAUGGAAGUU UGAGGCAAUA ACAGGUCUGU GAUGCCC UU AGACGUUCUG GGCCGCACGC GCGCUACACU GACGGAGCCA GCGAGUCUAA CCUUGGCCGA GAGGUCUUGG UAAUCUUG U GAAACUCCGU CGUGCUGGGG AUAGAGCAUU GUAAUUAUUG CUCUUCAACG AGGAAUUCCU AGUAAGCGCA AGUCAUCAG CUUGCGUUGA UUACGUCCCU GCCCUUUGUA CACACCGCCC GUCGCUAGUA CCGAUUGAAU GGCUUAGUGA GGCCUCAGGA UCUGCUUAG AGAAGGGGGC AACUCCAUCU CAGAGCGGAG AAUUUGGACA AACUUGGUCA UUUAGAGGAA CUAAAAGUCG U AACAAGGU UUCCGUAGGU GAACCUGCGG AAGGAUCAUU

GENBANK: GENBANK: CP022977.1

Macromolecule #2: mRNA

• Macromolecule: Name: mRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 1.923237 KDa

Sequence

String:

AUGAAA

Macromolecule #24: P-site tRNA

• Macromolecule: Name: P-site tRNA / type: rna / ID: 24 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 24.482807 KDa

Sequence

String:

AGCGCCGU(1MG)(2MG) CGCAGUGGAA GCGC(M2G)CAGGG CUCAU(T6A)ACCC UGAU(G7M)U(5MC)CUC GGAUCG (1MA)AA CCGAGCGGCG CUACCA

Macromolecule #37: A-site tRNA

• Macromolecule: Name: A-site tRNA / type: rna / ID: 37 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 24.378408 KDa

Sequence

String:

GGGUCGUUAG CUCAGUUGGU AGAGCAGUUG ACUUUUAAUC AAUUGGUCGC AGGUUCGAAU CCUGCACGAC CCACCA

GENBANK: GENBANK: CP044069.1

Macromolecule #38: 25S rRNA

• Macromolecule: Name: 25S rRNA / type: rna / ID: 38 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 1.029020625 MDa

Sequence

String:

UUGACCUCAA AUCAGGUAGG AGUACCCGCU GAACUUAAGC AUAUCAAUAA GCGGAGGAAA AGAAACCAAC CGGGAUUGCC UUAGUAACG GCGAGUGAAG CGGCAAAAGC UCAAAUUUGA AAUCUGGUAC CUUCGGUGCC CGAGUUGUAA UUUGGAGAGG G CAACUUUG GGGCCGUUCC UUGUCUAUGU UCCUUGGAAC AGGACGUCAU AGAGGGUGAG AAUCCCGUGU GGCGAGGAGU GC GGUUCUU UGUAAAGUGC CUUCGAAGAG UCGAGUUGUU UGGGAAUGCA GCUCUAAGUG GGUGGUAAAU UCCAUCUAAA GCU AAAUAU UGGCGAGAGA CCGAUAGCGA ACAAGUACAG UGAUGGAAAG AUGAAAAGAA CUUUGAAAAG AGAGUGAAAA AGUA CGUGA AAUUGUUGAA AGGGAAGGGC AUUUGAUCAG ACAUGGUGUU UUGUAUUUCA CUGGCCAGCA UCAGUUUUGG UGGCA GGAU AAAUCCAUAG GAAUGUAGCU UGCCUCGGUA AGUAUUAUAG CCUGUGGGAA UACUGCCAGC UGGGACUGAG GACUGC GAC GUAAGUCAAG GAUGCUGGCA UAAUGGUUAU AUGCCGCCCG UCUUGAAACA CGGACCAAGG AGUCUAACGU CUAUGCG AG UGUUUGGGUG UAAAACCCAU ACGCGUAAUG AAAGUGAACG UAGGUUGGGG CCUCGCAAGA GGUGCACAAU CGACCGAU C CUGAUGUCUU CGGAUGGAUU UGAGUAAGAG CAUAGCUGUU GGGACCCGAA AGAUGGUGAA CUAUGCCUGA AUAGGGUGA AGCCAGAGGA AACUCUGGUG GAGGCUCGUA GCGGUUCUGA CGUGCAAAUC GAUCGUCGAA UUUGGGUAUA GGGGCGAAAG ACUAAUCGA ACCAUCUAGU AGCUGGUUCC UGCCGAAGUU UCCCUCAGGA UAGCAGAAGC UCGUAUCAGU UUUAUGAGGU A AAGCGAAU GAUUAGAGGU UCCGGGGUCG AAAUGACCUU GACCUAUUCU CAAACUUUAA AUAUGUAAGA AGUCCUUGUU AC UUAAUUG AACGUGGACA UUUGAAUGAA GAGCUUUUAG UGGGCCAUUU UUGGUAAGCA GAACUGGCGA UGCGGGAUGA ACC GAACGU AGAGUUAAGG UGCCGGAAUA CACGCUCAUC AGACACCACA AAAGGUGUUA GUUCAUCUAG ACAGCCGGAC GGUG GCCAU GGAAGUCGGA AUCCGCUAAG GAGUGUGUAA CAACUCACCG GCCGAAUGAA CUAGCCCUGA AAAUGGAUGG CGCUC AAGC GUGUUACCUA UACUCUACCG UCAGGGUUGA UAUGAUGCCC UGACGAGUAG GCAGGCGUGG AGGUCAGUGA CGAAGC CUA GACCGUAAGG UCGGGUCGAA CGGCCUCUAG UGCAGAUCUU GGUGGUAGUA GCAAAUAUUC AAAUGAGAAC UUUGAAG AC UGAAGUGGGG AAAGGUUCCA CGUCAACAGC AGUUGGACGU GGGUUAGUCG AUCCUAAGAG AUGGGGAAGC UCCGUUUC A AAGGCCUGAU UUUAUGCAGG CCACCAUCGA AAGGGAAUCC GGUUAAGAUU CCGGAACCUG GAUAUGGAUU CUUCACGGU AACGUAACUG AAUGUGGAGA CGUCGGCGCG AGCCCUGGGA GGAGUUAUCU UUUCUUCUUA ACAGCUUAUC ACCCCGGAAU UGGUUUAUC CGGAGAUGGG GUCUUAUGGC UGGAAGAGGC CAGCACCUUU GCUGGCUCCG GUGCGCUUGU GACGGCCCGU G AAAAUCCA CAGGAAGGAA UAGUUUUCAU GCCAGGUCGU ACUGAUAACC GCAGCAGGUC UCCAAGGUGA ACAGCCUCUA GU UGAUAGA AUAAUGUAGA UAAGGGAAGU CGGCAAAAUA GAUCCGUAAC UUCGGGAUAA GGAUUGGCUC UAAGGGUCGG GUA CGAUCA ACUUAGAACU GGUACGGACA AGGGGAAUCU GACUGUCUAA UUAAAACAUA GCAUUGCGAU GGUCAGAAAG UGAU GUUGA CGCAAUGUGA UUUCUGCCCA GUGCUCUGAA UGUCAAAGUG AAGAAAUUCA ACCAAGCGCG GGUAAACGGC GGGAG UAAC UAUGACUCUC UUAAGGUAGC CAAAUGCCUC GUCAUCUAAU UAGUGACGCG CAUGAAUGGA UUAACGAGAU UCCCAC UGU CCCUAUCUAC UAUCUAGCGA AACCACAGCC AAGGGAACGG GCUUGGCAGA AUCAGCGGGG AAAGAAGACC CUGUUGA GC UUGACUCUAG UUUGACAUUG UGAAGAGACA UAGAGGGCUA CCUUUAUAGU UUCUUUACUU AUUCAAUGAA GCGGAGCU G GAAUUCAUUU UCCACGUUCU AGCAUUCAAG GUCCCAUUCG GGGCUGAUCC GGGUUGAAGA CAUUGUCAGG UGGGGAGUU UGGCUGGGGC GGCACAUCUG UUAAACGAUA ACGCAGAUGU CCUAAGGGGG GCUCAUGGAG AACAGAAAUC UCCAGUAGAA CAAAAGGGU AAAAGCCCCC UUGAUUUUGA UUUUCAGUGU GAAUACAAAC CAUGAAAGUG UGGCCUAUCG AUCCUUUAGU C CCUCGGAA UUUGAGGCUA GAGGUGCCAG AAAAGUUACC ACAGGGAUAA CUGGCUUGUG GCAGUCAAGC GUUCAUAGCG AC AUUGCUU UUUGAUUCUU CGAUGUCGGC UCUUCCUAUC AUACCGAAGC AGAAUUCGGU AAGCGUUGGA UUGUUCACCC ACU AAUAGG GAACGUGAGC UGGGUUUAGA CCGUCGUGAG ACAGGUUAGU UUUACCCUAC UGAUGAAUGU UACCGCAAUA GUAA UUGAA CUUAGUACGA GAGGAACAGU UCAUUCGGAU AAUUGGUUUU UGCGGCUGUC UGAUCAGGCA UUGCCGCGAA GCUAC CAUC CGCUGGAUUA UGGCUGAACG CCUCUAAGUC AGAAUCCAUG CUAGAACGCG GUGAUUUCUU UGCUCCACAC AAUAUA GAU GGAUACGAAU AAGGCGUCCU UGUGGCGUCG CUGAACCAUA GCAGGCUAGC AACGGUGCAC UUGGCGGAAA GGCCUUG GG UGCUUGCUGG CGAAUUGCAA UGUCAUUUUG CGUGGGGAUA AAUCAUUUGU AUACGACUUA GAUGUACAAC GGGGUAUU G UAAGCAGUAG AGUAGCCUUG UUGUUACGAU CUGCUGAGAU UAAGCCUUUG UUGUCUGAUU UGU

Macromolecule #39: 5S rRNA

• Macromolecule: Name: 5S rRNA / type: rna / ID: 39 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 38.951105 KDa

Sequence

String:

GGUUGCGGCC AUAUCUACCA GAAAGCACCG UUUCCCGUCC GAUCAACUGU AGUUAAGCUG GUAAGAGCCU GACCGAGUAG UGUAGUGGG UGACCAUACG CGAAACUCAG GUGCUGCAAU CU

GENBANK: GENBANK: CP022977.1

Macromolecule #40: 5.8S rRNA

• Macromolecule: Name: 5.8S rRNA / type: rna / ID: 40 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 50.682922 KDa

Sequence

String:

AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAAUGCGAU ACGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC CUUGGUAUUC CAGGGGGCAU GCCUGUUUGA GCGUCAUUU

GENBANK: GENBANK: MG817620.1

Macromolecule #3: 40S ribosomal protein S0-A

• Macromolecule: Name: 40S ribosomal protein S0-A / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 22.811074 KDa

Sequence

String:

SLPATFDLTP EDAQLLLAAN THLGARNVQV HQEPYVFNAR PDGVHVINVG KTWEKLVLAA RIIAAIPNPE DVVAISSRTF GQRAVLKFA AHTGATPIAG RFTPGSFTNY ITRSFKEPRL VIVTDPRSDA QAIKEASYVN IPVIALTDLD SPSEFVDVAI P CNNRGKHS IGLIWYLLAR EVLRLRGALV DRTQPWSIMP DLYFYRDP

UniProtKB: Small ribosomal subunit protein uS2A

Macromolecule #4: 40S ribosomal protein S1-A

• Macromolecule: Name: 40S ribosomal protein S1-A / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 26.314684 KDa

Sequence

String:

AVGKNKRLSK GKKGQKKRVV DPFTRKEWFD IKAPSTFENR NVGKTLVNKS TGLKSASDAL KGRVVEVCLA DLQGSEDHSF RKIKLRVDE VQGKNLLTNF HGMDFTTDKL RSMVRKWQTL IEANVTVKTS DDYVLRIFAI AFTRKQANQV KRHSYAQSSH I RAIRKVIS EILTKEVQGS TLAQLTSKLI PEVINKEIEN ATKDIFPLQN IHVRKVKLLK QPKFDVGALM ALHG

UniProtKB: Small ribosomal subunit protein eS1A

Macromolecule #5: 40S ribosomal protein S15

• Macromolecule: Name: 40S ribosomal protein S15 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.265591 KDa

Sequence

String:

KTHSYRGVDL EKLLEMSTED FVKLAPARVR RRFARGMTSK PAGFMKKLRA AKLAAPENEK PAPVRTHMRN MIIVPEMIGS VVGIYNGKA FNQVEIRPEM LGHYLGEFSI TYTPVRHG

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #6: 40S ribosomal protein S2

• Macromolecule: Name: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 23.08485 KDa

Sequence

String:

GWVPVTKLGR LVKAGKITTI EEIFLHSLPV KEFQIIDTLL PGLQDEVMNI KPVQKQTRAG QRTRFKAVVV VGDSNGHVGL GIKTAKEVA GAIRAGIIIA KLSVIPIRRG YWGTNLGQPH SLATKTTGKC GSVTVRLIPA PRGSGIVASP AVKKLLQLAG V EDVYTQSN GKTRTLENTL KAAFVAIGNT YGFLTPNLWA EQPLPVSPLD IYSDEASA

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #7: 40S ribosomal protein S3

• Macromolecule: Name: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 24.631713 KDa

Sequence

String:

LISKKRKLVA DGVFYAELNE FFTRELAEEG YSGVEVRVTP TKTEVIIRAT RTQDVLGENG RRINELTLLV QKRFKYAPGT IVLYAERVQ DRGLSAVAQA ESMKFKLLNG LAIRRAAYGV VRYVMESGAK GCEVVVSGKL RAARAKAMKF ADGFLIHSGQ P VNDFIDTA TRHVLMRQGV LGIKVKIMRD PAKSRTGPKA LPDAVTIIEP KEEEPILAPS VKDY

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #8: 40S ribosomal protein S4-A

• Macromolecule: Name: 40S ribosomal protein S4-A / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 29.167926 KDa

Sequence

String:

ARGPKKHLKR LAAPHHWLLD KLSGCYAPRP SAGPHKLRES LPLIVFLRNR LKYALNGREV KAILMQRHVK VDGKVRTDTT YPAGFMDVI TLDATNENFR LVYDVKGRFA VHRITDEEAS YKLGKVKKVQ LGKKGVPYVV THDGRTIRYP DPNIKVNDTV K IDLASGKI TDFIKFDAGK LVYVTGGRNL GRIGTIVHKE RHDGGFDLVH IKDSLDNTFV TRLNNVFVIG EQGKPYISLP KG KGIKLSI AEERDRRRAQ Q

UniProtKB: Small ribosomal subunit protein eS4A

Macromolecule #9: Rps5p

• Macromolecule: Name: Rps5p / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 22.908338 KDa

Sequence

String:

FTPVVLATPI PEEVQQAQTE IKLFNKWSFE EVEVKDASLV DYVQVRQPIF VAHTAGRYAN KRFRKAQCPI IERLTNSLMM NGRNNGKKL KAVRIIKHTL DIINVLTDQN PIQVVVDAIT NTGPREDTTR VGGGGAARRQ AVDVSPLRRV NQAIALLTIG A REAAFRNI KTIAETLAEE LINAAKGSST SYAIKKKDEL ERVAKSNR

UniProtKB: Rps5p

Macromolecule #10: 40S ribosomal protein S6-A

• Macromolecule: Name: 40S ribosomal protein S6-A / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 26.181445 KDa

Sequence

String:

MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT YTKAPKIQRL VTPQRLQRKR HQRALKVRNA QAQREAAAEY AQLLAKRLSE RKAEKAEIRK

UniProtKB: Small ribosomal subunit protein eS6A

Macromolecule #11: 40S ribosomal protein S7-A

• Macromolecule: Name: 40S ribosomal protein S7-A / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.000492 KDa

Sequence

String:

PQAKILSQAP TELELQVAQA FVELENSSPE LKAELRPLQF KSIREIDVAG GKKALAIFVP VPSLAGFHKV QTKLTRELEK KFQDRHVIF LAERRILPKP SRTSRQVQKR PRSRTLTAVH DKILEDLVFP TEIVGKRVRY LVGGNKIQKV LLDSKDVQQI D YKLESFQA VYNKLTGKQI VFEIPS

UniProtKB: Small ribosomal subunit protein eS7A

Macromolecule #12: 40S ribosomal protein S8

• Macromolecule: Name: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.018 KDa

Sequence

String:

GISRDSRHKR SATGAKRAQF RKKRKFELGR QPANTKIGAK RIHSVRTRGG NKKYRALRIE TGNFSWASEG ISKKTRIAGV VYHPSNNEL VRTNTLTKAA IVQIDATPFR QWFEAHYGQT LGKKSKNAER KWAARAASAK IESSVESQFS AGRLYACISS R PGQSGRCD GYILEGEELA FYLRRLTAK

UniProtKB: 40S ribosomal protein S8

Macromolecule #13: 40S ribosomal protein S9-A

• Macromolecule: Name: 40S ribosomal protein S9-A / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.081549 KDa

Sequence

String:

PRAPRTYSKT YSTPKRPYES SRLDAELKLA GEFGLKNKKE IYRISFQLSK IRRAARDLLT RDEKDPKRLF EGNALIRRLV RVGVLSEDK KKLDYVLALK VEDFLERRLQ TQVYKLGLAK SVHHARVLIT QRHIAVGKQI VNIPSFMVRL DSEKHIDFAP T SPFGGARP GRVARRNAAR KAEASG

UniProtKB: Small ribosomal subunit protein uS4A

Macromolecule #14: 40S ribosomal protein S10-A

• Macromolecule: Name: 40S ribosomal protein S10-A / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 11.042562 KDa

Sequence

String:

MLMPKEDRNK IHQYLFQEGV VVAKKDFNQA KHEEIDTKNL YVIKALQSLT SKGYVKTQFS WQYYYYTLTE EGVEYLREYL NLPEHIVPG TYI

UniProtKB: Small ribosomal subunit protein eS10A

Macromolecule #15: 40S ribosomal protein S11-A

• Macromolecule: Name: 40S ribosomal protein S11-A / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 16.272149 KDa

Sequence

String:

ELTVQSERAF QKQPHIFNNP KVKTSKRTKR WYKNAGLGFK TPKTAIEGSY IDKKCPFTGL VSIRGKILTG TVVSTKMHRT IVIRRAYLH YIPKYNRYEK RHKNVPVHVS PAFRVQVGDI VTVGQCRPIS KTVRFNVVKV SAA

UniProtKB: Small ribosomal subunit protein uS17A

Macromolecule #16: 40S ribosomal protein S12

• Macromolecule: Name: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.028008 KDa

Sequence

String:

TIEDALKVVL RTALVHDGLA RGLRESTKAL TRGEALLVVL VSSVTEANII KLVEGLANDP ENKVPLIKVA DAKQLGEWAG LGKIDREGN ARKVVGASVV VVKNWGAETD ELSMIMEHFS QQ

UniProtKB: Small ribosomal subunit protein eS12

Macromolecule #17: 40S ribosomal protein S13

• Macromolecule: Name: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 16.928748 KDa

Sequence

String:

GRMHSAGKGI SSSAIPYSRN APAWFKLSSE SVIEQIVKYA RKGLTPSQIG VLLRDAHGVT QARVITGNKI MRILKSNGLA PEIPEDLYY LIKKAVSVRK HLERNRKDKD AKFRLILIES RIHRLARYYR TVAVLPPNWK YESATASALV N

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #18: 40S ribosomal protein S14-B

• Macromolecule: Name: 40S ribosomal protein S14-B / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.446426 KDa

Sequence

String:

SQVFGVARIY ASFNDTFVHV TDLSGKETIA RVTGGMKVKA DRDESSPYAA MLAAQDVAAK CKEVGITAVH VKIRATGGTR TKTPGPGGQ AALRALARSG LRIGRIEDVT PVPSDSTRKK GGRRGRRL

UniProtKB: Small ribosomal subunit protein uS11B

Macromolecule #19: 40S ribosomal protein S16-A

• Macromolecule: Name: 40S ribosomal protein S16-A / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 15.659216 KDa

Sequence

String:

AVPSVQTFGK KKSATAVAHV KAGKGLIKVN GSPITLVEPE ILRFKVYEPL LLVGLDKFSN IDIRVRVTGG GHVSQVYAIR QAIAKGLVA YHQKYVDEQS KNELKKAFTS YDRTLLIADS RRPEPKKFGG KGARSRFQKS YR

UniProtKB: Small ribosomal subunit protein uS9A

Macromolecule #20: 40S ribosomal protein S17-B

• Macromolecule: Name: 40S ribosomal protein S17-B / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.268545 KDa

Sequence

String:

GRVRTKTVKR ASKALIERYY PKLTLDFQTN KRLCDEIATI QSKRLRNKIA GYTTHLMKRI QKGPVRGISF KLQEEERERK DQYVPEVSA LDLSRSNGVL NVDNQTSDLV KSLGLKLPLS VINVSA

UniProtKB: Small ribosomal subunit protein eS17B

Macromolecule #21: 40S ribosomal protein S18-A

• Macromolecule: Name: 40S ribosomal protein S18-A / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 16.940443 KDa

Sequence

String:

SLVVQEQGSF QHILRLLNTN VDGNIKIVYA LTTIKGVGRR YSNLVCKKAD VDLHKRAGEL TQEELERIVQ IMQNPTHYKI PAWFLNRQN DITDGKDYHT LANNVESKLR DDLERLKKIR AHRGIRHFWG LRVRGQHTKT TGRRRA

UniProtKB: Small ribosomal subunit protein uS13A

Macromolecule #22: 40S ribosomal protein S19-A

• Macromolecule: Name: 40S ribosomal protein S19-A / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 15.81093 KDa

Sequence

String:

PGVSVRDVAA QDFINAYASF LQRQGKLEVP GYVDIVKTSS GNEMPPQDAE GWFYKRAASV ARHIYMRKQV GVGKLNKLYG GAKSRGVRP YKHIDASGSI NRKVLQALEK IGIVEISPKG GRRISENGQR DLDRIAAQTL EEDE

UniProtKB: Small ribosomal subunit protein eS19A

Macromolecule #23: 40S ribosomal protein S20

• Macromolecule: Name: 40S ribosomal protein S20 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 11.363321 KDa

Sequence

String:

IKIRITLTST KVKQLENVSS NIVKNAEQHN LVKKGPVRLP TKVLKISTRK TPNGEGSKTW ETYEMRIHKR YIDLEAPVQI VKRITQITI EPGVDVEVVV A

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #25: 40S ribosomal protein S21-A

• Macromolecule: Name: 40S ribosomal protein S21-A / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 9.758829 KDa

Sequence

String:

MENDKGQLVE LYVPRKCSAT NRIIKADDHA SVQINVAKVD EEGRAIPGEY VTYALSGYVR SRGESDDSLN RLAQNDGLLK NVWSYSR

UniProtKB: Small ribosomal subunit protein eS21A

Macromolecule #26: 40S ribosomal protein S22-A

• Macromolecule: Name: 40S ribosomal protein S22-A / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.518867 KDa

Sequence

String:

TRSSVLADAL NAINNAEKTG KRQVLIRPSS KVIIKFLQVM QKHGYIGEFE YIDDHRSGKI VVQLNGRLNK CGVISPRFNV KIGDIEKWT ANLLPARQFG YVILTTSAGI MDHEEARRKH VSGKILGFVY

UniProtKB: Small ribosomal subunit protein uS8A

Macromolecule #27: 40S ribosomal protein S23-A

• Macromolecule: Name: 40S ribosomal protein S23-A / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 15.942699 KDa

Sequence

String:

GKGKPRGLNS ARKLRVHRRN NRWAENNYKK RLLGTAFKSS PFGGSSHAKG IVLEKLGIES KQPNSAIRKC VRVQLIKNGK KVTAFVPND GCLNFVDEND EVLLAGFGRK GKAKGDIPGV RFKVVKVSGV SLLALWKEKK EKPRS

UniProtKB: Small ribosomal subunit protein uS12A

Macromolecule #28: 40S ribosomal protein S24-A

• Macromolecule: Name: 40S ribosomal protein S24-A / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 15.23165 KDa

Sequence

String:

SDAVTIRTRK VISNPLLARK QFVVDVLHPN RANVSKDELR EKLAEVYKAE KDAVSVFGFR TQFGGGKSVG FGLVYNSVAE AKKFEPTYR LVRYGLAEKV EKASRQQRKQ KKNRDKKIFG TGKRLAKKVA RRNAD

UniProtKB: Small ribosomal subunit protein eS24A

Macromolecule #29: 40S ribosomal protein S25-A

• Macromolecule: Name: 40S ribosomal protein S25-A / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 9.540301 KDa

Sequence

String:

KKKWSKKSMK DRAQHAVILD QEKYDRILKE VPTYRYVSVS VLVDRLKIGG SLARIALRHL EKEGIIKPIS KHSKQAIYTR AT

UniProtKB: Small ribosomal subunit protein eS25A

Macromolecule #30: 40S ribosomal protein S26-B

• Macromolecule: Name: 40S ribosomal protein S26-B / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 11.022989 KDa

Sequence

String:

PKKRASNGRN KKGRGHVKPV RCVNCSKSIP KDKAIKRMAI RNIVEAAAVR DLSEASVYPE YALPKTYNKL HYCVSCAIHA RIVRVRSRE DRKNRAPP

UniProtKB: Small ribosomal subunit protein eS26B

Macromolecule #31: 40S ribosomal protein S27-A

• Macromolecule: Name: 40S ribosomal protein S27-A / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 8.762195 KDa

Sequence

String:

VLVQDLLHPT AASEARKHKL KTLVQGPRSY FLDVKCPGCL NITTVFSHAQ TAVTCESCST ILCTPTGGKA KLSEGTSFRR K

UniProtKB: Small ribosomal subunit protein eS27A

Macromolecule #32: 40S ribosomal protein S29-A

• Macromolecule: Name: 40S ribosomal protein S29-A / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 6.335303 KDa

Sequence

String:

ENVWFSHPRR YGKGSRQCRV CSSHTGLIRK YGLNICRQCF REKANDIGFN KFR

UniProtKB: Small ribosomal subunit protein uS14A

Macromolecule #33: 40S ribosomal protein S30-A

• Macromolecule: Name: 40S ribosomal protein S30-A / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 6.779086 KDa

Sequence

String:

AKVHGSLARA GKVKSQTPKV EKTEKPKKPK GRAYKRLLYT RRFVNVTLVN GKRRMNPGPS

UniProtKB: Small ribosomal subunit protein eS30A

Macromolecule #34: Ubiquitin-40S ribosomal protein S31

• Macromolecule: Name: Ubiquitin-40S ribosomal protein S31 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 8.329946 KDa

Sequence

String:

RKKKVYTTPK KIKHKHKAVK LAVLSYYKVD AEGKVTKLRR ECSNPTCGAG VFLANHKDRL YCGKCHSVYK VNA

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

Macromolecule #35: Guanine nucleotide-binding protein subunit beta-like protein

• Macromolecule: Name: Guanine nucleotide-binding protein subunit beta-like protein; type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 34.151484 KDa

Sequence

String:

EVLVLRGTLE GHNGWVTSLA TSAGQPNLLL SASRDKTLIS WKLTGDDQKF GVPVRSFKGH SHIVQDCTLT ADGAYALSAS WDKTLRLWD VATGETYQRF VGHKSDVMSV DIDKKASMII SGSRDKTIKV WTIKGQCLAT LLGHNDWVSQ VRVVPNEKAD D DSVTIISA GNDKMVKAWN LNQFQIEADF IGHNSNINTL TASPDGTLIA SAGKDGEIML WNLAAKKAMY TLSAQDEVFS LA FSPNRYW LAAATATGIK VFSLDPQYLV DDLRPEFAGY SKAAEPHAVS LAWSADGQTL FAGYTDNVIR VWQVM

UniProtKB: Small ribosomal subunit protein RACK1

Macromolecule #36: 40S ribosomal protein S28-A

• Macromolecule: Name: 40S ribosomal protein S28-A / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 7.116281 KDa

Sequence

String:

TPVTLAKVIK VLGRTGSRGG VTQVRVEFLE DTSRTIVRNV KGPVRENDIL VLMESEREAR RLR

UniProtKB: Small ribosomal subunit protein eS28A

Macromolecule #41: 60S ribosomal protein L2-A

• Macromolecule: Name: 60S ribosomal protein L2-A / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 27.089158 KDa

Sequence

String:

GRVIRNQRKG AGSIFTSHTR LRQGAAKLRT LDYAERHGYI RGIVKQIVHD SGRGAPLAKV VFRDPYKYRL REEIFIANEG VHTGQFIYA GKKASLNVGN VLPLGSVPEG TIVSNVEEKP GDRGALARAS GNYVIIIGHN PDENKTRVRL PSGAKKVISS D ARGVIGVI AGGGRVDKPL LKAGRAFHKY RLKRNSWPKT RGVAMNPVDH PHGGGNHQHI GKASTISRGA VSGQKAGLIA AR RTGLLRG SQKT

UniProtKB: Large ribosomal subunit protein uL2A

Macromolecule #42: 60S ribosomal protein L3

• Macromolecule: Name: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 43.719602 KDa

Sequence

String:

SHRKYEAPRH GHLGFLPRKR AASIRARVKA FPKDDRSKPV ALTSFLGYKA GMTTIVRDLD RPGSKFHKRE VVEAVTVVDT PPVVVVGVV GYVETPRGLR SLTTVWAEHL SDEVKRRFYK NWYKSKKKAF TKYSAKYAQD GAGIERELAR IKKYASVVRV L VHTQIRKT PLAQKKAHLA EIQLNGGSIS EKVDWAREHF EKTVAVDSVF EQNEMIDAIA VTKGHGFEGV THRWGTKKLP RK THRGLRK VACIGAWHPA HVMWSVARAG QRGYHSRTSI NHKIYRVGKG DDEANGATSF DRTKKTITPM GGFVHYGEIK NDF IMVKGC IPGNRKRIVT LRKSLYTNTS RKALEEVSLK WIDTASKFGK GRFQTPAEKH AFMGTLKKDL

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #43: 60S ribosomal protein L4-A

• Macromolecule: Name: 60S ribosomal protein L4-A / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 39.027926 KDa

Sequence

String:

SRPQVTVHSL TGEATANALP LPAVFSAPIR PDIVHTVFTS VNKNKRQAYA VSEKAGHQTS AESWGTGRAV ARIPRVGGGG TGRSGQGAF GNMCRGGRMF APTKTWRKWN VKVNHNEKRY ATASAIAATA VASLVLARGH RVEKIPEIPL VVSTDLESIQ K TKEAVAAL KAVGAHSDLL KVLKSKKLRA GKGKYRNRRW TQRRGPLVVY AEDNGIVKAL RNVPGVETAN VASLNLLQLA PG AHLGRFV IWTEAAFTKL DQVWGSETVA SSKVGYTLPS HIISTSDVTR IINSSEIQSA IRPAGQATQK RTHVLKKNPL KNK QVLLRL NPYAKVFAAE KLGSKKAEKT GTKPAAVFTE TLKHD

UniProtKB: Large ribosomal subunit protein uL4A

Macromolecule #44: 60S ribosomal protein L5

• Macromolecule: Name: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 33.415387 KDa

Sequence

String:

QKDAKSSAYS SRFQTPFRRR REGKTDYYQR KRLVTQHKAK YNTPKYRLVV RFTNKDIICQ IISSTITGDV VLAAAYSHEL PRYGITHGL TNWAAAYATG LLIARRTLQK LGLDETYKGV EEVEGEYELT EAVEDGPRPF KVFLDIGLQR TTTGARVFGA L KGASDGGL YVPHSENRFP GWDFETEEID PELLRSYIFG GHVSQYMEEL ADDDEERFSE LFKGYLADDI DADSLEDIYT SA HEAIRAD PAFKPTEKKF TKEQYAAESK KYRQTKLSKE ERAARVAAKI AALAGQQ

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #45: 60S ribosomal protein L6-B

• Macromolecule: Name: 60S ribosomal protein L6-B / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 19.893346 KDa

Sequence

String:

TAQQAPKWYP SEDVAAPKKT RKAVRPQKLR ASLVPGTVLI LLAGRFRGKR VVYLKHLEDN TLLVTGPFKV NGVPLRRVNA RYVIATSTK VSVEGVNVEK FNVEYFAKEK LTKKEKKEAN LFPEQQTKEI KTERVEDQKV VDKALLAEIK KTPLLKQYLS A SFSLKNGD KPHLLKF

UniProtKB: Large ribosomal subunit protein eL6B

Macromolecule #46: 60S ribosomal protein L7-A

• Macromolecule: Name: 60S ribosomal protein L7-A / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 25.240377 KDa

Sequence

String:

AEQVAAERAA RKAANKEKRA IILERNAAYQ KEYETAERNI IQAKRDAKAA GSYYVEAQHK LVFVVRIKGI NKIPPKPRKV LQLLRLTRI NSGTFVKVTK ATLELLKLIE PYVAYGYPSY STIRQLVYKR GFGKINKQRV PLSDNAIIEA NLGKYGILSI D DLIHEIIT VGPHFKQANN FLWPFKLSNP SGGWGVPRKF KHFIQGGSFG NREEFINKLV KSMN

UniProtKB: Large ribosomal subunit protein uL30A

Macromolecule #47: 60S ribosomal protein L8-A

• Macromolecule: Name: 60S ribosomal protein L8-A / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 25.814998 KDa

Sequence

String:

NPLTHSTPKN FGIGQAVQPK RNLSRYVKWP EYVRVQRQKK ILSIRLKVPP TIAQFQYTLD RNTAAETFKL FNKYRPETAA EKKERLTKE AAAVAEGKSK QDASPKPYAV KYGLNHVVAL IENKKAKLVL IANDVDPIEL VVFLPALCKK MGVPYAIVKG K ARLGTLVN QKTSAVAALT EVRAEDEAAL AKLVSTIDAN FADKYDEVKK HWGGGILGNK AQAKMDKRAK NSDSA

UniProtKB: Large ribosomal subunit protein eL8A

Macromolecule #48: 60S ribosomal protein L9-A

• Macromolecule: Name: 60S ribosomal protein L9-A / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.605061 KDa

Sequence

String:

MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYK MRYVYAHFPI NVNIVEKDGA KFIEVRNFLG DKKIRNVPVR DGVTIEFSTN VKDEIVLSGN SVEDVSQNAA D LQQICRVR NKDIRKFLDG IYVSHKGFIT EDL

UniProtKB: Large ribosomal subunit protein uL6A

Macromolecule #49: 60S ribosomal protein L10

• Macromolecule: Name: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 25.080062 KDa

Sequence

String:

ARRPARCYRY QKNKPYPKSR YNRAVPDSKI RIYDLGKKKA TVDEFPLCVH LVSNELEQLS SEALEAARIC ANKYMTTVSG RDAFHLRVR VHPFHVLRIN KMLSCAGADR LQQGMRGAWG KPHGLAARVD IGQIIFSVRT KDSNKDVVVE GLRRARYKFP G QQKIILSK KWGFTNLDRP EYLKKREAGE VKDDGAFVKF LSKKGSLENN IREFPEYFAA

UniProtKB: Large ribosomal subunit protein uL16

Macromolecule #50: 60S ribosomal protein L11-B

• Macromolecule: Name: 60S ribosomal protein L11-B / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 19.266082 KDa

Sequence

String:

QNPMRDLKIE KLVLNISVGE SGDRLTRASK VLEQLSGQTP VQSKARYTVR TFGIRRNEKI AVHVTVRGPK AEEILERGLK VKEYQLRDR NFSATGNFGF GIDEHIDLGI KYDPSIGIFG MDFYVVMNRP GARVTRRKRC KGTVGNSHKT TKEDTVSWFK Q KYDADVLD K

UniProtKB: Large ribosomal subunit protein uL5B

Macromolecule #51: 60S ribosomal protein L13-A

• Macromolecule: Name: 60S ribosomal protein L13-A / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.885234 KDa

Sequence

String:

AISKNLPILK NHFRKHWQER VKVHFDQAGK KVSRRNARAT RAAKIAPRPL DLLRPVVRAP TVKYNRKVRA GRGFTLAEVK AAGLTAAYA RTIGIAVDHR RQNRNQEIFD ANVQRLKEYQ SKIIVFPRNG KAPEAEQVLS AAATFPIAQP ATDVEARAVQ D NGESAFRT LRLARSEKKF RGIREKRARE KAEAE

UniProtKB: Large ribosomal subunit protein eL13A

Macromolecule #52: 60S ribosomal protein L14-A

• Macromolecule: Name: 60S ribosomal protein L14-A / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.976794 KDa

Sequence

String:

TDSIVKASNW RLVEVGRVVL IKKGQSAGKL AAIVEIIDQK KVLIDGPKAG VPRQAINLGQ VVLTPLTFAL PRGARTATVS KKWAAAAVC EKWAASSWAK KIAQRERRAA LTDFERFQVM VLRKQKRYTV KKALAKA

UniProtKB: Large ribosomal subunit protein eL14A

Macromolecule #53: 60S ribosomal protein L15-A

• Macromolecule: Name: 60S ribosomal protein L15-A / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 24.351162 KDa

Sequence

String:

GAYKYLEELQ RKKQSDVLRF LQRVRVWEYR QKNVIHRAAR PTRPDKARRL GYKAKQGFVI YRVRVRRGNR KRPVPKGATY GKPTNQGVN ELKYQRSLRA TAEERVGRRA ANLRVLNSYW VNQDSTYKYF EVILVDPQHK AIRRDARYNW ICDPVHKHRE A RGLTATGK KSRGINKGHK FNNTKAGRRK TWKRQNTLSL WRYRK

UniProtKB: Large ribosomal subunit protein eL15A

Macromolecule #54: 60S ribosomal protein L16-A

• Macromolecule: Name: 60S ribosomal protein L16-A / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 22.028951 KDa

Sequence

String:

VEPVVVIDGK GHLVGRLASV VAKQLLNGQK IVVVRAEELN ISGEFFRNKL KYHDFLRKAT AFNKTRGPFH FRAPSRIFYK ALRGMVSHK TARGKAALER LKVFEGIPPP YDKKKRVVVP QALRVLRLKP GRKYTTLGKL STSVGWKYED VVAKLEAKRK V SSAEYYAK KRAFTKKVAS ANATAAESDV AKQLAALGY

UniProtKB: Large ribosomal subunit protein uL13A

Macromolecule #55: 60S ribosomal protein L17-A

• Macromolecule: Name: 60S ribosomal protein L17-A / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 20.458322 KDa

Sequence

String:

ARYGATSTNP AKSASARGSY LRVSFKNTRE TAQAINGWEL TKAQKYLEQV LDHQRAIPFR RFNSSIGRTA QGKEFGVTKA RWPAKSVKF VQGLLQNAAA NAEAKGLDAT KLYVSHIQVN QAPKQRRRTY RAHGRINKYE SSPSHIELVV TEKEEAVAKA A EKKVVRLT SRQRGRIAAQ KRIAA

UniProtKB: Large ribosomal subunit protein uL22A

Macromolecule #56: 60S ribosomal protein L18-A

• Macromolecule: Name: 60S ribosomal protein L18-A / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 20.478059 KDa

Sequence

String:

GIDHTSKQHK RSGHRTAPKS DNVYLKLLVK LYTFLARRTD APFNKVVLKA LFLSKINRPP VSVSRIARAL KQEGAANKTV VVVGTVTDD ARIFEFPKTT VAALRFTAGA RAKIVKAGGE CITLDQLAVR APKGQNTLIL RGPRNSREAV RHFGMGPHKG K APRILSTG RKFERARGRR RSKGFKV

UniProtKB: Large ribosomal subunit protein eL18A

Macromolecule #57: 60S ribosomal protein L19-A

• Macromolecule: Name: 60S ribosomal protein L19-A / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.631121 KDa

Sequence

String:

ANLRTQKRLA ASVVGVGKRK VWLDPNETSE IAQANSRNAI RKLVKNGTIV KKAVTVHSKS RTRAHAQSKR EGRHSGYGKR KGTREARLP SQVVWIRRLR VLRRLLAKYR DAGKIDKHLY HVLYKESKGN AFKHKRALVE HIIQAKADAQ REKALNEEAE A RRLKNRAA RDRRAQRVAE KRDALLKEDA

UniProtKB: Large ribosomal subunit protein eL19A

Macromolecule #58: 60S ribosomal protein L20-A

• Macromolecule: Name: 60S ribosomal protein L20-A / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 20.347654 KDa

Sequence

String:

AHFKEYQVIG RRLPTESVPE PKLFRMRIFA SNEVIAKSRY WYFLQKLHKV KKASGEIVSI NQINEAHPTK VKNFGVWVRY DSRSGTHNM YKEIRDVSRV AAVETLYQDM AARHRARFRS IHILKVAEIE KTADVKRQYV KQFLTKDLKF PLPHRVQKST K TFSYKRPS TFY

UniProtKB: Large ribosomal subunit protein eL20A

Macromolecule #59: 60S ribosomal protein L21-A

• Macromolecule: Name: 60S ribosomal protein L21-A / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 18.148066 KDa

Sequence

String:

GKSHGYRSRT RYMFQRDFRK HGAVHLSTYL KVYKVGDIVD IKANGSIQKG MPHKFYQGKT GVVYNVTKSS VGVIINKMVG NRYLEKRLN LRVEHIKHSK CRQEFLERVK ANAAKRAEAK AQGVAVQLKR QPAQPRESRI VSTEGNVPQT LAPVPYETFI

UniProtKB: Large ribosomal subunit protein eL21A

Macromolecule #60: 60S ribosomal protein L22-A

• Macromolecule: Name: 60S ribosomal protein L22-A / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 11.263863 KDa

Sequence

String:

QKIAKTFTVD VSSPTENGVF DPASYAKYLI DHIKVEGAVG NLGNAVTVTE DGTVVTVVST AKFSGKYLKY LTKKYLKKNQ LRDWIRFVS TKTNEYRLAF Y

UniProtKB: Large ribosomal subunit protein eL22A

Macromolecule #61: 60S ribosomal protein L23-A

• Macromolecule: Name: 60S ribosomal protein L23-A / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.362755 KDa

Sequence

String:

SGNGAQGTKF RISLGLPVGA IMNCADNSGA RNLYIIAVKG SGSRLNRLPA ASLGDMVMAT VKKGKPELRK KVMPAIVVRQ AKSWRRRDG VFLYFEDNAG VIANPKGEMK GSAITGPVGK ECADLWPRVA SNSGVVV

UniProtKB: Large ribosomal subunit protein uL14A

Macromolecule #62: 60S ribosomal protein L24-A

• Macromolecule: Name: 60S ribosomal protein L24-A / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.64527 KDa

Sequence

String:

MKVEIDSFSG AKIYPGRGTL FVRGDSKIFR FQNSKSASLF KQRKNPRRIA WTVLFRKHHK KGITEEVAKK RSRKTVKAQR PITGASLDL IKERRSLKPE VRKANREEKL KANKEKKKAE KAARKAE

UniProtKB: Large ribosomal subunit protein eL24A

Macromolecule #63: 60S ribosomal protein L25

• Macromolecule: Name: 60S ribosomal protein L25 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.771176 KDa

Sequence

String:

KALKVRTSAT FRLPKTLKLA RAPKYASKAV PHYNRLDSYK VIEQPITSET AMKKVEDGNI LVFQVSMKAN KYQIKKAVKE LYEVDVLKV NTLVRPNGTK KAYVRLTADY DALDIANRIG YI

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #64: 60S ribosomal protein L26-A

• Macromolecule: Name: 60S ribosomal protein L26-A / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.005475 KDa

Sequence

String:

AKQSLDVSSD RRKARKAYFT APSSQRRVLL SAPLSKELRA QYGIKALPIR RDDEVLVVRG SKKGQEGKIS SVYRLKFAVQ VDKVTKEKV NGASVPINLH PSKLVITKLH LDKDRKALIQ RKGGKL

UniProtKB: Large ribosomal subunit protein uL24A

Macromolecule #65: 60S ribosomal protein L27-A

• Macromolecule: Name: 60S ribosomal protein L27-A / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 15.437166 KDa

Sequence

String:

AKFLKAGKVA VVVRGRYAGK KVVIVKPHDE GSKSHPFGHA LVAGIERYPL KVTKKHGAKK VAKRTKIKPF IKVVNYNHLL PTRYTLDVE AFKSVVSTET FEQPSQREEA KKVVKKAFEE RHQAGKNQWF FSKLRF

UniProtKB: Large ribosomal subunit protein eL27A

Macromolecule #66: 60S ribosomal protein L28

• Macromolecule: Name: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 16.630471 KDa

Sequence

String:

PSRFTKTRKH RGHVSAGKGR IGKHRKHPGG RGMAGGQHHH RINMDKYHPG YFGKVGMRYF HKQQAHFWKP VLNLDKLWTL IPEDKRDQY LKSASKETAP VIDTLAAGYG KILGKGRIPN VPVIVKARFV SKLAEEKIRA AGGVVELIA

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #67: 60S ribosomal protein L29

• Macromolecule: Name: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 6.560688 KDa

Sequence

String:

AKSKNHTAHN QTRKAHRNGI KKPKTYKYPS LKGVDPKFRR NHKHALHGTA KALAAAKK

UniProtKB: Large ribosomal subunit protein eL29

Macromolecule #68: 60S ribosomal protein L30

• Macromolecule: Name: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 10.487266 KDa

Sequence

String:

SINQKLALVI KSGKYTLGYK STVKSLRQGK SKLIIIAANT PVLRKSELEY YAMLSKTKVY YFQGGNNELG TAVGKLFRVG VVSILEAGD SDILTTL

UniProtKB: Large ribosomal subunit protein eL30

Macromolecule #69: 60S ribosomal protein L31-A

• Macromolecule: Name: 60S ribosomal protein L31-A / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 12.649754 KDa

Sequence

String:

LKDVVTREYT INLHKRLHGV SFKKRAPRAV KEIKKFAKLH MGTDDVRLAP ELNQAIWKRG VKGVEYRLRL RISRKRNEEE DAKNPLFSY VEPVLVASAK GLQTVVVEED

UniProtKB: Large ribosomal subunit protein eL31A

Macromolecule #70: 60S ribosomal protein L32

• Macromolecule: Name: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.478054 KDa

Sequence

String:

ASLPHPKIVK KHTKKFKRHH SDRYHRVAEN WRKQKGIDSV VRRRFRGNIS QPKIGYGSNK KTKFLSPSGH KTFLVANVKD LETLTMHTK TYAAEIAHNI SAKNRVVILA RAKALGIKVT NPKGRLAL

UniProtKB: Large ribosomal subunit protein eL32

Macromolecule #71: 60S ribosomal protein L33-A

• Macromolecule: Name: 60S ribosomal protein L33-A / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 12.045935 KDa

Sequence

String:

AESHRLYVKG KHLSYQRSKR VNNPNVSLIK IEGVATPQDA QFYLGKRIAY VYRASKEVRG SKIRVMWGKV TRTHGNSGVV RATFRNNLP AKTFGASVRI FLYPSNI

UniProtKB: Large ribosomal subunit protein eL33A

Macromolecule #72: 60S ribosomal protein L34-A

• Macromolecule: Name: 60S ribosomal protein L34-A / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 12.608831 KDa

Sequence

String:

AQRVTFRRRN PYNTRSNKIK VVKTPGGILR AQHVKKLATR PKCGDCGSAL QGISTLRPRQ YATVSKTHKT VSRAYGGSRC ANCVKERII RAFLIEEQKI VKKVVKEQTE AAK

UniProtKB: Large ribosomal subunit protein eL34A

Macromolecule #73: 60S ribosomal protein L35-A

• Macromolecule: Name: 60S ribosomal protein L35-A / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.811444 KDa

Sequence

String:

AGVKAYELRT KSKEQLASQL VDLKKELAEL KVQKLSRPSL PKIKTVRKSI ACVLTVINEQ QREAVRQLYK GKKYQPKDLR AKKTRALRR ALTKFEASQV TEKQRKKQIA FPQRKYAIKA

UniProtKB: Large ribosomal subunit protein uL29A

Macromolecule #74: 60S ribosomal protein L36-A

• Macromolecule: Name: 60S ribosomal protein L36-A / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 11.020063 KDa

Sequence

String:

TVKTGIAIGL NKGKKVTSMT PAPKISYKKG AASNRTKFVR SLVREIAGLS PYERRLIDLI RNSGEKRARK VAKKRLGSFT RAKAKVEEM NNIIAASRRH

UniProtKB: Large ribosomal subunit protein eL36A

Macromolecule #75: 60S ribosomal protein L37-A

• Macromolecule: Name: 60S ribosomal protein L37-A / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 9.229632 KDa

Sequence

String:

GKGTPSFGKR HNKSHTLCNR CGRRSFHVQK KTCSSCGYPA AKTRSYNWGA KAKRRHTTGT GRMRYLKHVS RRFKNGFQTG S

UniProtKB: Large ribosomal subunit protein eL37A

Macromolecule #76: 60S ribosomal protein L38

• Macromolecule: Name: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 8.714363 KDa

Sequence

String:

AREITDIKQF LELTRRADVK TATVKINKKL NKAGKPFRQT KFKVRGSSSL YTLVINDAGK AKKLIQSLPP TLKVNRL

UniProtKB: Large ribosomal subunit protein eL38

Macromolecule #77: 60S ribosomal protein L39

• Macromolecule: Name: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 6.227444 KDa

Sequence

String:

AAQKSFRIKQ KMAKAKKQNR PLPQWIRLRT NNTIRYNAKR RNWRRTKMNI

UniProtKB: Large ribosomal subunit protein eL39

Macromolecule #78: Ubiquitin-60S ribosomal protein L40

• Macromolecule: Name: Ubiquitin-60S ribosomal protein L40 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 6.032321 KDa

Sequence

String:

IIEPSLKALA SKYNCDKSVC RKCYARLPPR ATNCRKRKCG HTNQLRPKKK LK

UniProtKB: Ubiquitin-ribosomal protein eL40A fusion protein

Macromolecule #79: 60S ribosomal protein L41-A

• Macromolecule: Name: 60S ribosomal protein L41-A / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 3.354243 KDa

Sequence

String:

MRAKWRKKRT RRLKRKRRKV RARSK

Macromolecule #80: 60S ribosomal protein L42-A

• Macromolecule: Name: 60S ribosomal protein L42-A / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 11.840159 KDa

Sequence

String:

VNVPKTRKTY CKGKTCRKHT QHKVTQYKAG KASLFAQGKR RYDRKQSGFG GQTKPVFHKK AKTTKKVVLR LECVKCKTRA QLTLKRCKH FELGGEKKQK GQAL

UniProtKB: Large ribosomal subunit protein eL42A

Macromolecule #81: 60S ribosomal protein L43-A

• Macromolecule: Name: 60S ribosomal protein L43-A / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 9.981756 KDa

Sequence

String:

AKRTKKVGIT GKYGVRYGSS LRRQVKKLEI QQHARYDCSF CGKKTVKRGA AGIWTCSCCK KTVAGGAYTV STAAAATVRS TIRRLREMV EA

UniProtKB: Large ribosomal subunit protein eL43A

Macromolecule #82: Eukaryotic translation initiation factor 5A-1

• Macromolecule: Name: Eukaryotic translation initiation factor 5A-1 / type: protein_or_peptide / ID: 82 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 16.889045 KDa

Sequence

String:

EEHTFETADA GSSATYPMQC SALRKNGFVV IKSRPCKIVD MSTSKTG(5CT)HG HAKVHLVAID IFTGKKLEDL SPSTHN MEV PVVKRNEYQL LDIDDGFLSL MNMDGDTKDD VKAPEGELGD SLQTAFDEGK DLMVTIISAM GEEAAISFKE AARTD

UniProtKB: Eukaryotic translation initiation factor 5A-1

Macromolecule #83: 60S ribosomal protein L1

• Macromolecule: Name: 60S ribosomal protein L1 / type: protein_or_peptide / ID: 83 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.944343 KDa

Sequence

String:

AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA A AAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AA

Macromolecule #84: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 84 / Number of copies: 7 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #85: SPERMIDINE

• Macromolecule: Name: SPERMIDINE / type: ligand / ID: 85 / Number of copies: 1 / Formula: SPD
• Molecular weight: Theoretical: 145.246 Da

Chemical component information

ChemComp-SPD:
SPERMIDINE

Macromolecule #86: 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethy...

• Macromolecule: Name: 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione; type: ligand / ID: 86 / Number of copies: 1 / Formula: 3HE
• Molecular weight: Theoretical: 281.347 Da

Chemical component information

ChemComp-3HE:
4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 28.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: EMDB MAP
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 50989
• Initial angle assignment: Type: PROJECTION MATCHING
• Final angle assignment: Type: PROJECTION MATCHING